Mongoose feces-associated gemycircularvirus a
Average proteome isoelectric point is 7.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0E3M076|A0A0E3M076_9VIRU Capsid OS=Mongoose feces-associated gemycircularvirus a OX=1634485 PE=4 SV=1
MM1 pKa = 7.32 TFSFNARR8 pKa = 11.84 YY9 pKa = 9.72 ALLTYY14 pKa = 8.83 AQCGTLDD21 pKa = 3.4 PWSVVHH27 pKa = 7.01 HH28 pKa = 5.91 IAEE31 pKa = 4.27 LRR33 pKa = 11.84 GEE35 pKa = 4.3 CIIARR40 pKa = 11.84 EE41 pKa = 4.03 AHH43 pKa = 6.55 ADD45 pKa = 3.57 GGTHH49 pKa = 5.97 LHH51 pKa = 6.23 SFIDD55 pKa = 3.87 FGRR58 pKa = 11.84 KK59 pKa = 7.99 FRR61 pKa = 11.84 SRR63 pKa = 11.84 RR64 pKa = 11.84 ADD66 pKa = 2.96 IFDD69 pKa = 3.92 VDD71 pKa = 3.78 GCHH74 pKa = 6.5 PNVSPTHH81 pKa = 5.44 STPQAGFDD89 pKa = 3.9 YY90 pKa = 10.68 ACKK93 pKa = 10.71 DD94 pKa = 3.18 GDD96 pKa = 3.8 IVAGGLSRR104 pKa = 11.84 PDD106 pKa = 3.78 AGPIPAKK113 pKa = 9.83 HH114 pKa = 6.47 DD115 pKa = 3.18 RR116 pKa = 11.84 WHH118 pKa = 7.68 DD119 pKa = 3.08 ITAAKK124 pKa = 10.16 SRR126 pKa = 11.84 EE127 pKa = 4.08 EE128 pKa = 3.95 FFQLLLEE135 pKa = 4.32 HH136 pKa = 7.27 APRR139 pKa = 11.84 DD140 pKa = 3.47 LCTSFNSLSKK150 pKa = 10.61 YY151 pKa = 9.77 ADD153 pKa = 2.51 WMYY156 pKa = 11.4 RR157 pKa = 11.84 DD158 pKa = 4.69 DD159 pKa = 4.36 PAPYY163 pKa = 10.13 KK164 pKa = 10.57 HH165 pKa = 7.38 DD166 pKa = 4.14 DD167 pKa = 3.58 QLEE170 pKa = 4.43 GSLGPFPEE178 pKa = 4.39 LSAWVQGSIDD188 pKa = 3.86 EE189 pKa = 4.58 LNSLVLYY196 pKa = 10.41 GPSRR200 pKa = 11.84 MGKK203 pKa = 6.35 TLWARR208 pKa = 11.84 SHH210 pKa = 6.62 GSHH213 pKa = 7.02 AYY215 pKa = 10.06 FGGLFSLDD223 pKa = 3.79 EE224 pKa = 4.41 PLQGANYY231 pKa = 10.76 AVFDD235 pKa = 5.42 DD236 pKa = 4.4 INGGIQFFPQYY247 pKa = 10.22 KK248 pKa = 8.34 WWLGHH253 pKa = 5.19 QAQFYY258 pKa = 9.15 ATDD261 pKa = 3.41 KK262 pKa = 10.98 YY263 pKa = 10.53 KK264 pKa = 10.88 GKK266 pKa = 10.54 RR267 pKa = 11.84 LIHH270 pKa = 5.94 WGKK273 pKa = 8.31 PAIWVANDD281 pKa = 3.79 DD282 pKa = 3.54 PRR284 pKa = 11.84 EE285 pKa = 3.83 QHH287 pKa = 5.94 GADD290 pKa = 4.42 RR291 pKa = 11.84 EE292 pKa = 4.27 WLEE295 pKa = 4.12 ANCLFVYY302 pKa = 9.25 VHH304 pKa = 6.6 SPLYY308 pKa = 10.85 SNLVV312 pKa = 3.3
Molecular weight: 35.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.609
IPC2_protein 5.537
IPC_protein 5.601
Toseland 6.109
ProMoST 5.995
Dawson 5.906
Bjellqvist 5.906
Wikipedia 5.931
Rodwell 5.893
Grimsley 6.3
Solomon 5.906
Lehninger 5.893
Nozaki 6.186
DTASelect 6.376
Thurlkill 6.389
EMBOSS 6.351
Sillero 6.275
Patrickios 3.719
IPC_peptide 5.919
IPC2_peptide 6.3
IPC2.peptide.svr19 6.173
Protein with the highest isoelectric point:
>tr|A0A0E3M076|A0A0E3M076_9VIRU Capsid OS=Mongoose feces-associated gemycircularvirus a OX=1634485 PE=4 SV=1
MM1 pKa = 7.82 AFRR4 pKa = 11.84 SRR6 pKa = 11.84 YY7 pKa = 7.78 SSRR10 pKa = 11.84 SRR12 pKa = 11.84 RR13 pKa = 11.84 RR14 pKa = 11.84 PLRR17 pKa = 11.84 RR18 pKa = 11.84 RR19 pKa = 11.84 YY20 pKa = 9.6 RR21 pKa = 11.84 KK22 pKa = 8.76 IRR24 pKa = 11.84 RR25 pKa = 11.84 SFRR28 pKa = 11.84 RR29 pKa = 11.84 GARR32 pKa = 11.84 RR33 pKa = 11.84 YY34 pKa = 7.33 MRR36 pKa = 11.84 RR37 pKa = 11.84 SMTRR41 pKa = 11.84 RR42 pKa = 11.84 KK43 pKa = 9.89 VADD46 pKa = 3.27 IASTKK51 pKa = 10.74 CKK53 pKa = 10.89 DD54 pKa = 3.45 NMLALPLTGDD64 pKa = 3.7 LGTPGVAGEE73 pKa = 3.9 AAIVQGAFISGYY85 pKa = 9.0 LFCPTARR92 pKa = 11.84 SSEE95 pKa = 3.92 WTEE98 pKa = 3.93 GEE100 pKa = 4.25 RR101 pKa = 11.84 EE102 pKa = 4.15 VTRR105 pKa = 11.84 QQSRR109 pKa = 11.84 VFARR113 pKa = 11.84 GYY115 pKa = 9.95 KK116 pKa = 9.44 EE117 pKa = 4.25 RR118 pKa = 11.84 INIVTSDD125 pKa = 3.38 STNWIWRR132 pKa = 11.84 RR133 pKa = 11.84 IVFSTHH139 pKa = 3.59 NRR141 pKa = 11.84 LWEE144 pKa = 4.22 AFPPGTVEE152 pKa = 4.27 KK153 pKa = 9.93 YY154 pKa = 9.28 YY155 pKa = 10.87 EE156 pKa = 4.57 GGTGVFQPGQTRR168 pKa = 11.84 AMWNFAPSLGGAPAAAVNYY187 pKa = 10.36 AVFEE191 pKa = 4.34 GEE193 pKa = 5.8 RR194 pKa = 11.84 GNDD197 pKa = 2.88 WLNFMNATTNKK208 pKa = 9.93 KK209 pKa = 9.63 FIKK212 pKa = 10.51 VMSDD216 pKa = 2.89 VTQKK220 pKa = 11.4 LEE222 pKa = 4.09 GTNDD226 pKa = 3.23 RR227 pKa = 11.84 PRR229 pKa = 11.84 QYY231 pKa = 10.84 HH232 pKa = 5.31 FNRR235 pKa = 11.84 WYY237 pKa = 10.12 PFNKK241 pKa = 9.67 NFTYY245 pKa = 10.62 NEE247 pKa = 4.16 KK248 pKa = 10.5 EE249 pKa = 4.08 RR250 pKa = 11.84 GDD252 pKa = 3.79 TKK254 pKa = 10.68 PSQDD258 pKa = 3.72 YY259 pKa = 10.36 QSKK262 pKa = 10.22 FSSNEE267 pKa = 3.46 IGSMGDD273 pKa = 3.4 VYY275 pKa = 11.64 VLDD278 pKa = 5.11 LFNSANGEE286 pKa = 4.07 VGNQLGFQCHH296 pKa = 4.73 GTYY299 pKa = 9.9 YY300 pKa = 9.42 WHH302 pKa = 7.23 EE303 pKa = 4.14 KK304 pKa = 9.62
Molecular weight: 35.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.301
IPC2_protein 9.633
IPC_protein 10.262
Toseland 10.204
ProMoST 10.028
Dawson 10.423
Bjellqvist 10.16
Wikipedia 10.643
Rodwell 10.643
Grimsley 10.511
Solomon 10.467
Lehninger 10.423
Nozaki 10.204
DTASelect 10.145
Thurlkill 10.277
EMBOSS 10.628
Sillero 10.35
Patrickios 10.131
IPC_peptide 10.467
IPC2_peptide 9.063
IPC2.peptide.svr19 8.645
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
616
304
312
308.0
35.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.117 ± 0.815
1.461 ± 0.32
6.006 ± 1.611
4.87 ± 0.487
5.844 ± 0.052
8.442 ± 0.075
3.409 ± 1.412
3.734 ± 0.3
4.383 ± 0.372
6.494 ± 1.496
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.786 ± 0.571
4.545 ± 1.15
4.87 ± 0.622
3.571 ± 0.032
8.442 ± 2.072
6.981 ± 0.173
4.87 ± 1.153
4.545 ± 0.484
2.922 ± 0.196
4.708 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here