Pseudoruegeria sp. SK021

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Pseudoruegeria; unclassified Pseudoruegeria

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3609 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X4IUN9|A0A1X4IUN9_9RHOB Peptide ABC transporter permease OS=Pseudoruegeria sp. SK021 OX=1933035 GN=BV911_14265 PE=3 SV=1
MM1 pKa = 7.72SDD3 pKa = 3.26ILVKK7 pKa = 10.71SSSEE11 pKa = 3.74LVQALRR17 pKa = 11.84QATGGDD23 pKa = 4.03TIFLAPGDD31 pKa = 4.15YY32 pKa = 7.67EE33 pKa = 4.35TLFIKK38 pKa = 10.65DD39 pKa = 3.36LAYY42 pKa = 10.53ASDD45 pKa = 3.77VTLTSLDD52 pKa = 3.68AEE54 pKa = 4.37NPAVFTAPVTFVRR67 pKa = 11.84ANGLVLDD74 pKa = 4.72NIIFQSDD81 pKa = 4.16SIEE84 pKa = 4.27GTSGSAPLLFLHH96 pKa = 6.66SSEE99 pKa = 4.26NVTMTDD105 pKa = 3.69LVFTGYY111 pKa = 10.52LPSEE115 pKa = 4.4EE116 pKa = 4.68EE117 pKa = 4.27GVDD120 pKa = 3.31AYY122 pKa = 10.88AATTTRR128 pKa = 11.84GDD130 pKa = 3.85AITGYY135 pKa = 10.75GYY137 pKa = 8.36QTGLRR142 pKa = 11.84IHH144 pKa = 6.63NSDD147 pKa = 3.45NVSLEE152 pKa = 3.88DD153 pKa = 3.25ATFRR157 pKa = 11.84DD158 pKa = 3.86LRR160 pKa = 11.84YY161 pKa = 9.97AIYY164 pKa = 9.48TGEE167 pKa = 4.09STEE170 pKa = 3.92VDD172 pKa = 3.56LTGMSIEE179 pKa = 4.03QVRR182 pKa = 11.84EE183 pKa = 4.01GIMFYY188 pKa = 10.18EE189 pKa = 4.28VQGMVISDD197 pKa = 3.62SVFQNFKK204 pKa = 9.82PWLGMDD210 pKa = 3.79DD211 pKa = 3.85PAYY214 pKa = 8.93DD215 pKa = 4.22TNTHH219 pKa = 6.28DD220 pKa = 4.49HH221 pKa = 7.45PDD223 pKa = 3.75MIQYY227 pKa = 9.42WGDD230 pKa = 3.16NSTLGVHH237 pKa = 7.73DD238 pKa = 3.5ITITGNTFIAEE249 pKa = 4.43PGGSTQTIFGHH260 pKa = 7.11LGTGPAGVTASNFTVTDD277 pKa = 3.27NTIINGHH284 pKa = 5.69PHH286 pKa = 7.42AISLGDD292 pKa = 3.21VDD294 pKa = 4.47GAVISGNVLLPNSDD308 pKa = 3.77GLLKK312 pKa = 10.58YY313 pKa = 7.73WAPSIGAPGGQNLEE327 pKa = 4.06IYY329 pKa = 10.68DD330 pKa = 3.83NVVVYY335 pKa = 9.74WNGYY339 pKa = 7.2GVWALDD345 pKa = 3.62EE346 pKa = 4.22QEE348 pKa = 4.02RR349 pKa = 11.84AAANVSIGDD358 pKa = 3.76NTLLSDD364 pKa = 5.38DD365 pKa = 5.14PDD367 pKa = 5.05DD368 pKa = 3.9EE369 pKa = 5.0NYY371 pKa = 9.89WGFWSPASDD380 pKa = 3.77YY381 pKa = 11.39PLVQWAEE388 pKa = 3.92EE389 pKa = 4.07AGLGTVLGTEE399 pKa = 4.04TGEE402 pKa = 4.23VLWASSHH409 pKa = 5.64GSFIEE414 pKa = 4.11GLGGDD419 pKa = 3.87DD420 pKa = 3.92TLSGRR425 pKa = 11.84AGTDD429 pKa = 2.57ILTGGGGADD438 pKa = 3.01RR439 pKa = 11.84FLYY442 pKa = 10.5DD443 pKa = 3.12MRR445 pKa = 11.84GATVQGDD452 pKa = 4.27DD453 pKa = 5.66LILDD457 pKa = 4.33LDD459 pKa = 4.15FSEE462 pKa = 5.57GDD464 pKa = 3.24SLYY467 pKa = 10.56ILTGTMGLFSDD478 pKa = 4.68SAISGNDD485 pKa = 3.13LRR487 pKa = 11.84VLGDD491 pKa = 3.6GSSAIVDD498 pKa = 3.62SFADD502 pKa = 3.11IGEE505 pKa = 4.37LVASGAMIYY514 pKa = 10.73DD515 pKa = 3.43SDD517 pKa = 4.14GLGGGVLSLTSGDD530 pKa = 3.21GHH532 pKa = 7.46ALYY535 pKa = 10.23IAGLDD540 pKa = 3.6AASLPEE546 pKa = 4.17DD547 pKa = 4.43TIDD550 pKa = 3.93PTPADD555 pKa = 4.51PEE557 pKa = 4.15PDD559 pKa = 3.26ILGTAAGEE567 pKa = 4.28TLWASSRR574 pKa = 11.84GSFIDD579 pKa = 3.75ARR581 pKa = 11.84EE582 pKa = 4.06GDD584 pKa = 3.66DD585 pKa = 3.45TLFGRR590 pKa = 11.84TGADD594 pKa = 2.46ILMGGDD600 pKa = 3.21GADD603 pKa = 2.68RR604 pKa = 11.84FVFDD608 pKa = 5.09LRR610 pKa = 11.84SSSAAEE616 pKa = 4.29DD617 pKa = 4.53DD618 pKa = 5.64LILDD622 pKa = 4.56LDD624 pKa = 4.11FSEE627 pKa = 5.21GDD629 pKa = 3.49EE630 pKa = 4.9IYY632 pKa = 10.54ILTNTAGQFTDD643 pKa = 3.18SHH645 pKa = 7.23DD646 pKa = 4.03PGNDD650 pKa = 3.13LRR652 pKa = 11.84ILSDD656 pKa = 3.49GASVMITSLADD667 pKa = 3.56LQEE670 pKa = 4.46VVASGAMTYY679 pKa = 10.94DD680 pKa = 3.14SDD682 pKa = 4.07GDD684 pKa = 3.67SGGFLRR690 pKa = 11.84LADD693 pKa = 3.55GTGYY697 pKa = 11.17GLQIEE702 pKa = 4.74DD703 pKa = 5.41LYY705 pKa = 10.97PLLL708 pKa = 5.35

Molecular weight: 74.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X4J1T7|A0A1X4J1T7_9RHOB DUF4329 domain-containing protein OS=Pseudoruegeria sp. SK021 OX=1933035 GN=BV911_01190 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.82IINSRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.39GRR39 pKa = 11.84AKK41 pKa = 10.69LSAA44 pKa = 3.92

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3609

0

3609

1135785

28

4002

314.7

34.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.259 ± 0.055

0.902 ± 0.013

6.215 ± 0.044

5.044 ± 0.032

3.756 ± 0.023

8.591 ± 0.038

2.006 ± 0.02

5.293 ± 0.033

2.849 ± 0.032

10.358 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.706 ± 0.02

2.544 ± 0.023

5.266 ± 0.027

3.298 ± 0.025

6.57 ± 0.046

5.483 ± 0.028

5.843 ± 0.039

7.432 ± 0.035

1.396 ± 0.017

2.189 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski