Adoxophyes orana granulovirus (AoGV)
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 119 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q7T9Y1|Q7T9Y1_GVAO ADOR34 OS=Adoxophyes orana granulovirus OX=170617 GN=ORF_34 PE=4 SV=1
MM1 pKa = 7.72 IPVLDD6 pKa = 4.3 FNYY9 pKa = 10.08 VPADD13 pKa = 4.4 DD14 pKa = 5.85 IMDD17 pKa = 3.92 ISNNTVSGDD26 pKa = 3.55 NEE28 pKa = 4.36 INLEE32 pKa = 4.18 DD33 pKa = 4.83 ADD35 pKa = 3.71 IQKK38 pKa = 10.88 FIDD41 pKa = 3.08 QTRR44 pKa = 11.84 NVNSYY49 pKa = 9.83 CANSKK54 pKa = 10.2 HH55 pKa = 6.11 FNLFVDD61 pKa = 6.62 FILNDD66 pKa = 3.73 NITYY70 pKa = 10.86 SNFFII75 pKa = 5.63
Molecular weight: 8.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.738
IPC2_protein 3.706
IPC_protein 3.668
Toseland 3.439
ProMoST 3.884
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.694
Rodwell 3.503
Grimsley 3.363
Solomon 3.668
Lehninger 3.617
Nozaki 3.834
DTASelect 4.113
Thurlkill 3.541
EMBOSS 3.706
Sillero 3.795
Patrickios 1.888
IPC_peptide 3.656
IPC2_peptide 3.757
IPC2.peptide.svr19 3.739
Protein with the highest isoelectric point:
>tr|Q7T9U9|Q7T9U9_GVAO ORF_66 OS=Adoxophyes orana granulovirus OX=170617 GN=ORF_66 PE=4 SV=1
MM1 pKa = 7.41 VYY3 pKa = 10.06 RR4 pKa = 11.84 RR5 pKa = 11.84 RR6 pKa = 11.84 PGRR9 pKa = 11.84 PRR11 pKa = 11.84 SRR13 pKa = 11.84 SRR15 pKa = 11.84 SRR17 pKa = 11.84 SRR19 pKa = 11.84 SRR21 pKa = 11.84 SRR23 pKa = 11.84 SRR25 pKa = 11.84 SYY27 pKa = 11.14 SRR29 pKa = 11.84 QQTHH33 pKa = 5.05 ARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 SRR39 pKa = 11.84 SRR41 pKa = 11.84 SPYY44 pKa = 8.71 RR45 pKa = 11.84 RR46 pKa = 11.84 RR47 pKa = 11.84 SRR49 pKa = 11.84 HH50 pKa = 4.39 INQYY54 pKa = 8.27 VV55 pKa = 2.79
Molecular weight: 6.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.36
IPC2_protein 11.155
IPC_protein 12.384
Toseland 12.53
ProMoST 13.027
Dawson 12.53
Bjellqvist 12.53
Wikipedia 13.013
Rodwell 12.032
Grimsley 12.574
Solomon 13.027
Lehninger 12.925
Nozaki 12.53
DTASelect 12.53
Thurlkill 12.53
EMBOSS 13.027
Sillero 12.53
Patrickios 11.754
IPC_peptide 13.042
IPC2_peptide 12.018
IPC2.peptide.svr19 9.115
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
118
1
119
30941
48
1138
260.0
30.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.652 ± 0.12
2.611 ± 0.154
6.477 ± 0.223
5.553 ± 0.256
4.667 ± 0.169
3.022 ± 0.187
2.098 ± 0.081
7.889 ± 0.143
7.734 ± 0.319
9.476 ± 0.203
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.553 ± 0.113
9.45 ± 0.18
3.032 ± 0.234
3.436 ± 0.157
3.949 ± 0.164
6.183 ± 0.154
5.543 ± 0.213
6.593 ± 0.193
0.84 ± 0.066
5.242 ± 0.151
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here