Amino acid dipepetide frequency for Azoarcus indigens

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.367AlaAla: 20.367 ± 0.206	1.378AlaCys: 1.378 ± 0.033	7.135AlaAsp: 7.135 ± 0.068	8.31AlaGlu: 8.31 ± 0.092	4.187AlaPhe: 4.187 ± 0.056	12.482AlaGly: 12.482 ± 0.131	2.622AlaHis: 2.622 ± 0.045	5.2AlaIle: 5.2 ± 0.059	2.948AlaLys: 2.948 ± 0.056	16.023AlaLeu: 16.023 ± 0.127	3.269AlaMet: 3.269 ± 0.056	2.761AlaAsn: 2.761 ± 0.051	6.178AlaPro: 6.178 ± 0.08	4.741AlaGln: 4.741 ± 0.06	9.945AlaArg: 9.945 ± 0.09	6.613AlaSer: 6.613 ± 0.074	5.855AlaThr: 5.855 ± 0.071	9.098AlaVal: 9.098 ± 0.077	1.897AlaTrp: 1.897 ± 0.037	2.696AlaTyr: 2.696 ± 0.043	0.0AlaXaa: 0.0 ± 0.0
Cys	1.193CysAla: 1.193 ± 0.03	0.142CysCys: 0.142 ± 0.01	0.483CysAsp: 0.483 ± 0.014	0.495CysGlu: 0.495 ± 0.02	0.336CysPhe: 0.336 ± 0.015	1.034CysGly: 1.034 ± 0.026	0.293CysHis: 0.293 ± 0.012	0.36CysIle: 0.36 ± 0.015	0.212CysLys: 0.212 ± 0.012	0.949CysLeu: 0.949 ± 0.025	0.171CysMet: 0.171 ± 0.011	0.227CysAsn: 0.227 ± 0.013	0.521CysPro: 0.521 ± 0.02	0.245CysGln: 0.245 ± 0.011	0.726CysArg: 0.726 ± 0.024	0.486CysSer: 0.486 ± 0.017	0.409CysThr: 0.409 ± 0.016	0.644CysVal: 0.644 ± 0.024	0.144CysTrp: 0.144 ± 0.01	0.232CysTyr: 0.232 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	6.745AspAla: 6.745 ± 0.074	0.509AspCys: 0.509 ± 0.021	2.764AspAsp: 2.764 ± 0.041	3.416AspGlu: 3.416 ± 0.048	2.156AspPhe: 2.156 ± 0.033	5.043AspGly: 5.043 ± 0.063	1.073AspHis: 1.073 ± 0.027	2.489AspIle: 2.489 ± 0.039	1.633AspLys: 1.633 ± 0.036	5.254AspLeu: 5.254 ± 0.057	1.105AspMet: 1.105 ± 0.029	1.278AspAsn: 1.278 ± 0.028	2.901AspPro: 2.901 ± 0.048	1.398AspGln: 1.398 ± 0.029	3.514AspArg: 3.514 ± 0.051	2.43AspSer: 2.43 ± 0.041	2.434AspThr: 2.434 ± 0.045	3.527AspVal: 3.527 ± 0.047	0.975AspTrp: 0.975 ± 0.025	1.562AspTyr: 1.562 ± 0.035	0.0AspXaa: 0.0 ± 0.0
Glu	8.153GluAla: 8.153 ± 0.089	0.42GluCys: 0.42 ± 0.016	2.623GluAsp: 2.623 ± 0.038	3.348GluGlu: 3.348 ± 0.053	1.725GluPhe: 1.725 ± 0.033	4.379GluGly: 4.379 ± 0.055	1.379GluHis: 1.379 ± 0.031	2.943GluIle: 2.943 ± 0.048	1.878GluLys: 1.878 ± 0.045	6.63GluLeu: 6.63 ± 0.072	1.379GluMet: 1.379 ± 0.029	1.388GluAsn: 1.388 ± 0.03	2.464GluPro: 2.464 ± 0.045	2.649GluGln: 2.649 ± 0.043	5.51GluArg: 5.51 ± 0.075	2.546GluSer: 2.546 ± 0.043	2.77GluThr: 2.77 ± 0.045	4.569GluVal: 4.569 ± 0.067	0.775GluTrp: 0.775 ± 0.021	1.149GluTyr: 1.149 ± 0.032	0.0GluXaa: 0.0 ± 0.0
Phe	4.187PheAla: 4.187 ± 0.055	0.372PheCys: 0.372 ± 0.013	2.376PheAsp: 2.376 ± 0.044	2.031PheGlu: 2.031 ± 0.037	1.332PhePhe: 1.332 ± 0.032	3.372PheGly: 3.372 ± 0.047	0.848PheHis: 0.848 ± 0.025	1.537PheIle: 1.537 ± 0.034	0.848PheLys: 0.848 ± 0.025	3.23PheLeu: 3.23 ± 0.05	0.731PheMet: 0.731 ± 0.023	1.046PheAsn: 1.046 ± 0.028	1.604PhePro: 1.604 ± 0.035	0.991PheGln: 0.991 ± 0.027	2.247PheArg: 2.247 ± 0.04	2.174PheSer: 2.174 ± 0.043	1.668PheThr: 1.668 ± 0.035	2.574PheVal: 2.574 ± 0.04	0.476PheTrp: 0.476 ± 0.016	0.891PheTyr: 0.891 ± 0.023	0.0PheXaa: 0.0 ± 0.0
Gly	9.454GlyAla: 9.454 ± 0.096	0.971GlyCys: 0.971 ± 0.025	4.32GlyAsp: 4.32 ± 0.055	5.683GlyGlu: 5.683 ± 0.066	3.345GlyPhe: 3.345 ± 0.046	7.764GlyGly: 7.764 ± 0.122	1.909GlyHis: 1.909 ± 0.039	3.904GlyIle: 3.904 ± 0.043	3.111GlyLys: 3.111 ± 0.055	9.724GlyLeu: 9.724 ± 0.097	2.337GlyMet: 2.337 ± 0.041	2.28GlyAsn: 2.28 ± 0.049	2.952GlyPro: 2.952 ± 0.052	3.017GlyGln: 3.017 ± 0.05	6.595GlyArg: 6.595 ± 0.068	4.859GlySer: 4.859 ± 0.091	4.053GlyThr: 4.053 ± 0.067	6.391GlyVal: 6.391 ± 0.065	1.494GlyTrp: 1.494 ± 0.041	2.406GlyTyr: 2.406 ± 0.039	0.0GlyXaa: 0.0 ± 0.0
His	2.754HisAla: 2.754 ± 0.044	0.264HisCys: 0.264 ± 0.013	1.161HisAsp: 1.161 ± 0.028	1.06HisGlu: 1.06 ± 0.026	0.94HisPhe: 0.94 ± 0.026	2.142HisGly: 2.142 ± 0.04	0.636HisHis: 0.636 ± 0.023	0.893HisIle: 0.893 ± 0.024	0.506HisLys: 0.506 ± 0.018	2.325HisLeu: 2.325 ± 0.044	0.411HisMet: 0.411 ± 0.015	0.473HisAsn: 0.473 ± 0.016	1.474HisPro: 1.474 ± 0.034	0.688HisGln: 0.688 ± 0.021	1.617HisArg: 1.617 ± 0.033	1.003HisSer: 1.003 ± 0.029	0.96HisThr: 0.96 ± 0.026	1.345HisVal: 1.345 ± 0.027	0.367HisTrp: 0.367 ± 0.016	0.708HisTyr: 0.708 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	6.342IleAla: 6.342 ± 0.06	0.384IleCys: 0.384 ± 0.015	2.704IleAsp: 2.704 ± 0.041	2.767IleGlu: 2.767 ± 0.043	1.185IlePhe: 1.185 ± 0.032	4.042IleGly: 4.042 ± 0.052	0.936IleHis: 0.936 ± 0.024	1.401IleIle: 1.401 ± 0.034	1.197IleLys: 1.197 ± 0.032	3.662IleLeu: 3.662 ± 0.054	0.682IleMet: 0.682 ± 0.019	1.306IleAsn: 1.306 ± 0.029	2.038IlePro: 2.038 ± 0.038	1.231IleGln: 1.231 ± 0.03	2.802IleArg: 2.802 ± 0.05	2.267IleSer: 2.267 ± 0.037	2.227IleThr: 2.227 ± 0.04	3.194IleVal: 3.194 ± 0.05	0.427IleTrp: 0.427 ± 0.015	0.951IleTyr: 0.951 ± 0.027	0.0IleXaa: 0.0 ± 0.0
Lys	3.478LysAla: 3.478 ± 0.056	0.149LysCys: 0.149 ± 0.011	1.363LysAsp: 1.363 ± 0.032	1.498LysGlu: 1.498 ± 0.034	0.69LysPhe: 0.69 ± 0.023	2.092LysGly: 2.092 ± 0.045	0.563LysHis: 0.563 ± 0.02	1.167LysIle: 1.167 ± 0.029	0.987LysLys: 0.987 ± 0.028	3.215LysLeu: 3.215 ± 0.049	0.614LysMet: 0.614 ± 0.02	0.753LysAsn: 0.753 ± 0.02	1.765LysPro: 1.765 ± 0.037	1.071LysGln: 1.071 ± 0.026	2.131LysArg: 2.131 ± 0.038	1.323LysSer: 1.323 ± 0.037	1.433LysThr: 1.433 ± 0.035	2.344LysVal: 2.344 ± 0.041	0.311LysTrp: 0.311 ± 0.013	0.582LysTyr: 0.582 ± 0.022	0.0LysXaa: 0.0 ± 0.0
Leu	17.101LeuAla: 17.101 ± 0.121	1.062LeuCys: 1.062 ± 0.03	6.102LeuAsp: 6.102 ± 0.062	6.213LeuGlu: 6.213 ± 0.069	3.75LeuPhe: 3.75 ± 0.053	9.293LeuGly: 9.293 ± 0.09	2.314LeuHis: 2.314 ± 0.04	4.369LeuIle: 4.369 ± 0.052	3.236LeuLys: 3.236 ± 0.057	12.76LeuLeu: 12.76 ± 0.124	2.336LeuMet: 2.336 ± 0.043	2.688LeuAsn: 2.688 ± 0.045	7.044LeuPro: 7.044 ± 0.085	4.048LeuGln: 4.048 ± 0.057	8.703LeuArg: 8.703 ± 0.086	6.248LeuSer: 6.248 ± 0.067	5.239LeuThr: 5.239 ± 0.058	7.699LeuVal: 7.699 ± 0.075	1.303LeuTrp: 1.303 ± 0.031	2.285LeuTyr: 2.285 ± 0.042	0.0LeuXaa: 0.0 ± 0.0
Met	2.704MetAla: 2.704 ± 0.044	0.147MetCys: 0.147 ± 0.009	1.137MetAsp: 1.137 ± 0.027	1.173MetGlu: 1.173 ± 0.027	0.578MetPhe: 0.578 ± 0.021	1.582MetGly: 1.582 ± 0.037	0.458MetHis: 0.458 ± 0.017	0.824MetIle: 0.824 ± 0.023	0.894MetLys: 0.894 ± 0.026	2.57MetLeu: 2.57 ± 0.041	0.548MetMet: 0.548 ± 0.019	0.801MetAsn: 0.801 ± 0.019	1.392MetPro: 1.392 ± 0.032	0.895MetGln: 0.895 ± 0.027	1.658MetArg: 1.658 ± 0.032	1.588MetSer: 1.588 ± 0.034	1.193MetThr: 1.193 ± 0.026	1.583MetVal: 1.583 ± 0.033	0.192MetTrp: 0.192 ± 0.01	0.339MetTyr: 0.339 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	3.119AsnAla: 3.119 ± 0.048	0.276AsnCys: 0.276 ± 0.014	1.17AsnAsp: 1.17 ± 0.027	1.18AsnGlu: 1.18 ± 0.027	0.871AsnPhe: 0.871 ± 0.023	2.204AsnGly: 2.204 ± 0.048	0.48AsnHis: 0.48 ± 0.015	1.116AsnIle: 1.116 ± 0.031	0.672AsnLys: 0.672 ± 0.02	2.783AsnLeu: 2.783 ± 0.05	0.478AsnMet: 0.478 ± 0.017	0.664AsnAsn: 0.664 ± 0.025	1.751AsnPro: 1.751 ± 0.035	0.801AsnGln: 0.801 ± 0.024	1.862AsnArg: 1.862 ± 0.032	1.149AsnSer: 1.149 ± 0.034	1.34AsnThr: 1.34 ± 0.031	1.725AsnVal: 1.725 ± 0.04	0.415AsnTrp: 0.415 ± 0.016	0.695AsnTyr: 0.695 ± 0.026	0.0AsnXaa: 0.0 ± 0.0
Pro	8.323ProAla: 8.323 ± 0.107	0.377ProCys: 0.377 ± 0.017	2.994ProAsp: 2.994 ± 0.048	3.636ProGlu: 3.636 ± 0.054	1.731ProPhe: 1.731 ± 0.028	4.653ProGly: 4.653 ± 0.064	0.989ProHis: 0.989 ± 0.023	1.673ProIle: 1.673 ± 0.031	1.195ProLys: 1.195 ± 0.031	5.855ProLeu: 5.855 ± 0.062	1.063ProMet: 1.063 ± 0.026	1.133ProAsn: 1.133 ± 0.03	3.316ProPro: 3.316 ± 0.07	2.024ProGln: 2.024 ± 0.038	3.331ProArg: 3.331 ± 0.046	2.42ProSer: 2.42 ± 0.044	2.257ProThr: 2.257 ± 0.042	4.162ProVal: 4.162 ± 0.059	0.776ProTrp: 0.776 ± 0.022	1.171ProTyr: 1.171 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	5.332GlnAla: 5.332 ± 0.06	0.226GlnCys: 0.226 ± 0.012	1.5GlnAsp: 1.5 ± 0.03	1.725GlnGlu: 1.725 ± 0.032	1.023GlnPhe: 1.023 ± 0.027	2.876GlnGly: 2.876 ± 0.045	0.816GlnHis: 0.816 ± 0.026	1.561GlnIle: 1.561 ± 0.029	0.856GlnLys: 0.856 ± 0.025	4.076GlnLeu: 4.076 ± 0.056	0.836GlnMet: 0.836 ± 0.024	0.725GlnAsn: 0.725 ± 0.024	2.285GlnPro: 2.285 ± 0.044	1.676GlnGln: 1.676 ± 0.036	3.119GlnArg: 3.119 ± 0.048	1.571GlnSer: 1.571 ± 0.035	1.636GlnThr: 1.636 ± 0.031	2.668GlnVal: 2.668 ± 0.04	0.506GlnTrp: 0.506 ± 0.019	0.692GlnTyr: 0.692 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	8.327ArgAla: 8.327 ± 0.086	0.679ArgCys: 0.679 ± 0.022	3.847ArgAsp: 3.847 ± 0.051	4.815ArgGlu: 4.815 ± 0.071	3.092ArgPhe: 3.092 ± 0.042	5.361ArgGly: 5.361 ± 0.056	2.011ArgHis: 2.011 ± 0.04	3.792ArgIle: 3.792 ± 0.047	2.031ArgLys: 2.031 ± 0.033	9.721ArgLeu: 9.721 ± 0.086	1.845ArgMet: 1.845 ± 0.038	2.019ArgAsn: 2.019 ± 0.033	3.734ArgPro: 3.734 ± 0.067	3.147ArgGln: 3.147 ± 0.053	6.357ArgArg: 6.357 ± 0.074	3.811ArgSer: 3.811 ± 0.054	2.835ArgThr: 2.835 ± 0.045	5.025ArgVal: 5.025 ± 0.066	1.138ArgTrp: 1.138 ± 0.03	2.174ArgTyr: 2.174 ± 0.039	0.0ArgXaa: 0.0 ± 0.0
Ser	6.684SerAla: 6.684 ± 0.08	0.438SerCys: 0.438 ± 0.016	2.371SerAsp: 2.371 ± 0.036	2.575SerGlu: 2.575 ± 0.045	1.941SerPhe: 1.941 ± 0.039	5.39SerGly: 5.39 ± 0.085	1.138SerHis: 1.138 ± 0.027	2.073SerIle: 2.073 ± 0.042	1.201SerLys: 1.201 ± 0.028	5.898SerLeu: 5.898 ± 0.068	1.135SerMet: 1.135 ± 0.025	1.281SerAsn: 1.281 ± 0.033	2.898SerPro: 2.898 ± 0.043	1.653SerGln: 1.653 ± 0.031	3.997SerArg: 3.997 ± 0.053	3.037SerSer: 3.037 ± 0.074	2.622SerThr: 2.622 ± 0.048	3.564SerVal: 3.564 ± 0.056	0.726SerTrp: 0.726 ± 0.022	1.299SerTyr: 1.299 ± 0.035	0.0SerXaa: 0.0 ± 0.0
Thr	5.874ThrAla: 5.874 ± 0.076	0.376ThrCys: 0.376 ± 0.016	2.205ThrAsp: 2.205 ± 0.041	2.315ThrGlu: 2.315 ± 0.039	1.493ThrPhe: 1.493 ± 0.031	4.297ThrGly: 4.297 ± 0.059	0.98ThrHis: 0.98 ± 0.027	1.779ThrIle: 1.779 ± 0.034	0.988ThrLys: 0.988 ± 0.024	6.224ThrLeu: 6.224 ± 0.072	0.788ThrMet: 0.788 ± 0.022	1.045ThrAsn: 1.045 ± 0.033	3.323ThrPro: 3.323 ± 0.047	1.508ThrGln: 1.508 ± 0.033	3.204ThrArg: 3.204 ± 0.049	2.251ThrSer: 2.251 ± 0.048	2.482ThrThr: 2.482 ± 0.059	4.054ThrVal: 4.054 ± 0.063	0.608ThrTrp: 0.608 ± 0.019	1.017ThrTyr: 1.017 ± 0.028	0.0ThrXaa: 0.0 ± 0.0
Val	9.436ValAla: 9.436 ± 0.081	0.728ValCys: 0.728 ± 0.021	4.014ValAsp: 4.014 ± 0.044	4.624ValGlu: 4.624 ± 0.059	2.756ValPhe: 2.756 ± 0.047	5.6ValGly: 5.6 ± 0.073	1.384ValHis: 1.384 ± 0.032	3.266ValIle: 3.266 ± 0.044	2.129ValLys: 2.129 ± 0.042	8.141ValLeu: 8.141 ± 0.082	1.749ValMet: 1.749 ± 0.033	1.868ValAsn: 1.868 ± 0.036	3.623ValPro: 3.623 ± 0.049	2.322ValGln: 2.322 ± 0.039	5.049ValArg: 5.049 ± 0.07	4.159ValSer: 4.159 ± 0.061	3.561ValThr: 3.561 ± 0.058	6.062ValVal: 6.062 ± 0.073	0.862ValTrp: 0.862 ± 0.025	1.403ValTyr: 1.403 ± 0.029	0.0ValXaa: 0.0 ± 0.0
Trp	1.363TrpAla: 1.363 ± 0.031	0.147TrpCys: 0.147 ± 0.009	0.578TrpAsp: 0.578 ± 0.021	0.648TrpGlu: 0.648 ± 0.021	0.495TrpPhe: 0.495 ± 0.017	0.861TrpGly: 0.861 ± 0.025	0.364TrpHis: 0.364 ± 0.017	0.571TrpIle: 0.571 ± 0.02	0.425TrpLys: 0.425 ± 0.017	2.178TrpLeu: 2.178 ± 0.042	0.369TrpMet: 0.369 ± 0.015	0.413TrpAsn: 0.413 ± 0.016	0.666TrpPro: 0.666 ± 0.022	0.732TrpGln: 0.732 ± 0.023	1.325TrpArg: 1.325 ± 0.032	0.753TrpSer: 0.753 ± 0.023	0.648TrpThr: 0.648 ± 0.022	0.916TrpVal: 0.916 ± 0.024	0.24TrpTrp: 0.24 ± 0.012	0.296TrpTyr: 0.296 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.766TyrAla: 2.766 ± 0.05	0.26TyrCys: 0.26 ± 0.013	1.359TyrAsp: 1.359 ± 0.03	1.165TyrGlu: 1.165 ± 0.026	0.91TyrPhe: 0.91 ± 0.024	2.101TyrGly: 2.101 ± 0.041	0.533TyrHis: 0.533 ± 0.019	0.825TyrIle: 0.825 ± 0.026	0.584TyrLys: 0.584 ± 0.019	2.577TyrLeu: 2.577 ± 0.041	0.41TyrMet: 0.41 ± 0.017	0.604TyrAsn: 0.604 ± 0.023	1.15TyrPro: 1.15 ± 0.028	0.867TyrGln: 0.867 ± 0.022	2.066TyrArg: 2.066 ± 0.041	1.235TyrSer: 1.235 ± 0.03	1.19TyrThr: 1.19 ± 0.03	1.593TyrVal: 1.593 ± 0.033	0.356TyrTrp: 0.356 ± 0.015	0.654TyrTyr: 0.654 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4840 proteins (1613751 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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