Xanthomonas virus Cf1c
Average proteome isoelectric point is 8.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q38198|Q38198_9VIRU Zot domain-containing protein OS=Xanthomonas virus Cf1c OX=10862 PE=4 SV=1
MM1 pKa = 7.97 IGDD4 pKa = 3.81 TASISLLTGLPGSGKK19 pKa = 8.34 SLRR22 pKa = 11.84 IIQAIRR28 pKa = 11.84 YY29 pKa = 9.0 LMDD32 pKa = 4.18 KK33 pKa = 9.88 GAHH36 pKa = 5.53 VYY38 pKa = 9.57 VCNIDD43 pKa = 4.48 GISVPGTTPWADD55 pKa = 3.15 PHH57 pKa = 6.53 KK58 pKa = 10.23 WQDD61 pKa = 3.48 LPAGSILFVDD71 pKa = 4.95 EE72 pKa = 4.6 AQHH75 pKa = 6.13 FFPARR80 pKa = 11.84 RR81 pKa = 11.84 GGDD84 pKa = 3.26 PVEE87 pKa = 4.56 TIKK90 pKa = 11.15 AMSTIRR96 pKa = 11.84 HH97 pKa = 5.97 DD98 pKa = 3.9 GVRR101 pKa = 11.84 LVLATQQPNYY111 pKa = 10.18 LDD113 pKa = 3.38 TYY115 pKa = 10.94 LRR117 pKa = 11.84 GLVGYY122 pKa = 9.73 HH123 pKa = 5.24 EE124 pKa = 4.67 HH125 pKa = 7.76 LLRR128 pKa = 11.84 QSGKK132 pKa = 9.0 QKK134 pKa = 9.71 TFIFRR139 pKa = 11.84 NSQIIEE145 pKa = 4.26 EE146 pKa = 4.36 VRR148 pKa = 11.84 SPLPRR153 pKa = 11.84 IKK155 pKa = 10.39 KK156 pKa = 9.94 LYY158 pKa = 10.38 DD159 pKa = 3.24 YY160 pKa = 9.6 EE161 pKa = 4.33 VWKK164 pKa = 10.87 QPTEE168 pKa = 3.77 CFKK171 pKa = 10.57 FYY173 pKa = 10.94 KK174 pKa = 9.94 SAEE177 pKa = 4.12 VHH179 pKa = 4.39 TMKK182 pKa = 10.73 YY183 pKa = 10.3 QMPALVKK190 pKa = 9.54 KK191 pKa = 10.45 ALMILPVVALLAGGAWYY208 pKa = 9.99 AVYY211 pKa = 10.38 RR212 pKa = 11.84 DD213 pKa = 3.29 TMFAKK218 pKa = 10.52 KK219 pKa = 10.24 ADD221 pKa = 3.69 AAPANKK227 pKa = 8.2 TAPSGPSLAGTASAGAAARR246 pKa = 11.84 PKK248 pKa = 10.47 VNSAEE253 pKa = 4.32 DD254 pKa = 3.76 YY255 pKa = 10.77 VGQLVPLVADD265 pKa = 4.41 VPWSAPAYY273 pKa = 8.48 VDD275 pKa = 3.54 RR276 pKa = 11.84 PVVSDD281 pKa = 3.01 PHH283 pKa = 6.85 VYY285 pKa = 10.69 CMATEE290 pKa = 4.17 NTCRR294 pKa = 11.84 CVTEE298 pKa = 3.99 QNSRR302 pKa = 11.84 VVMRR306 pKa = 11.84 DD307 pKa = 3.27 DD308 pKa = 3.3 VCRR311 pKa = 11.84 DD312 pKa = 2.92 IARR315 pKa = 11.84 WGEE318 pKa = 3.85 PYY320 pKa = 10.66 NPYY323 pKa = 10.23 KK324 pKa = 10.21 PPHH327 pKa = 6.52 SPAQKK332 pKa = 9.84 QQDD335 pKa = 4.03 SPVAEE340 pKa = 4.11 ATQPKK345 pKa = 8.9 PQVPQQSGAVSSSVQRR361 pKa = 11.84 ATRR364 pKa = 11.84 SLGTFPEE371 pKa = 4.46 SPPYY375 pKa = 9.32 QTTTYY380 pKa = 9.45 TPPTTRR386 pKa = 11.84 DD387 pKa = 3.05 LL388 pKa = 4.02
Molecular weight: 42.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.347
IPC2_protein 8.331
IPC_protein 8.244
Toseland 8.565
ProMoST 8.697
Dawson 9.019
Bjellqvist 9.033
Wikipedia 9.151
Rodwell 9.107
Grimsley 8.873
Solomon 9.121
Lehninger 9.077
Nozaki 9.092
DTASelect 8.902
Thurlkill 8.96
EMBOSS 9.151
Sillero 9.165
Patrickios 4.291
IPC_peptide 9.107
IPC2_peptide 7.849
IPC2.peptide.svr19 7.736
Protein with the highest isoelectric point:
>tr|Q38198|Q38198_9VIRU Zot domain-containing protein OS=Xanthomonas virus Cf1c OX=10862 PE=4 SV=1
MM1 pKa = 7.97 IGDD4 pKa = 3.81 TASISLLTGLPGSGKK19 pKa = 8.34 SLRR22 pKa = 11.84 IIQAIRR28 pKa = 11.84 YY29 pKa = 9.0 LMDD32 pKa = 4.18 KK33 pKa = 9.88 GAHH36 pKa = 5.53 VYY38 pKa = 9.57 VCNIDD43 pKa = 4.48 GISVPGTTPWADD55 pKa = 3.15 PHH57 pKa = 6.53 KK58 pKa = 10.23 WQDD61 pKa = 3.48 LPAGSILFVDD71 pKa = 4.95 EE72 pKa = 4.6 AQHH75 pKa = 6.13 FFPARR80 pKa = 11.84 RR81 pKa = 11.84 GGDD84 pKa = 3.26 PVEE87 pKa = 4.56 TIKK90 pKa = 11.15 AMSTIRR96 pKa = 11.84 HH97 pKa = 5.97 DD98 pKa = 3.9 GVRR101 pKa = 11.84 LVLATQQPNYY111 pKa = 10.18 LDD113 pKa = 3.38 TYY115 pKa = 10.94 LRR117 pKa = 11.84 GLVGYY122 pKa = 9.73 HH123 pKa = 5.24 EE124 pKa = 4.67 HH125 pKa = 7.76 LLRR128 pKa = 11.84 QSGKK132 pKa = 9.0 QKK134 pKa = 9.71 TFIFRR139 pKa = 11.84 NSQIIEE145 pKa = 4.26 EE146 pKa = 4.36 VRR148 pKa = 11.84 SPLPRR153 pKa = 11.84 IKK155 pKa = 10.39 KK156 pKa = 9.94 LYY158 pKa = 10.38 DD159 pKa = 3.24 YY160 pKa = 9.6 EE161 pKa = 4.33 VWKK164 pKa = 10.87 QPTEE168 pKa = 3.77 CFKK171 pKa = 10.57 FYY173 pKa = 10.94 KK174 pKa = 9.94 SAEE177 pKa = 4.12 VHH179 pKa = 4.39 TMKK182 pKa = 10.73 YY183 pKa = 10.3 QMPALVKK190 pKa = 9.54 KK191 pKa = 10.45 ALMILPVVALLAGGAWYY208 pKa = 9.99 AVYY211 pKa = 10.38 RR212 pKa = 11.84 DD213 pKa = 3.29 TMFAKK218 pKa = 10.52 KK219 pKa = 10.24 ADD221 pKa = 3.69 AAPANKK227 pKa = 8.2 TAPSGPSLAGTASAGAAARR246 pKa = 11.84 PKK248 pKa = 10.47 VNSAEE253 pKa = 4.32 DD254 pKa = 3.76 YY255 pKa = 10.77 VGQLVPLVADD265 pKa = 4.41 VPWSAPAYY273 pKa = 8.48 VDD275 pKa = 3.54 RR276 pKa = 11.84 PVVSDD281 pKa = 3.01 PHH283 pKa = 6.85 VYY285 pKa = 10.69 CMATEE290 pKa = 4.17 NTCRR294 pKa = 11.84 CVTEE298 pKa = 3.99 QNSRR302 pKa = 11.84 VVMRR306 pKa = 11.84 DD307 pKa = 3.27 DD308 pKa = 3.3 VCRR311 pKa = 11.84 DD312 pKa = 2.92 IARR315 pKa = 11.84 WGEE318 pKa = 3.85 PYY320 pKa = 10.66 NPYY323 pKa = 10.23 KK324 pKa = 10.21 PPHH327 pKa = 6.52 SPAQKK332 pKa = 9.84 QQDD335 pKa = 4.03 SPVAEE340 pKa = 4.11 ATQPKK345 pKa = 8.9 PQVPQQSGAVSSSVQRR361 pKa = 11.84 ATRR364 pKa = 11.84 SLGTFPEE371 pKa = 4.46 SPPYY375 pKa = 9.32 QTTTYY380 pKa = 9.45 TPPTTRR386 pKa = 11.84 DD387 pKa = 3.05 LL388 pKa = 4.02
Molecular weight: 42.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.347
IPC2_protein 8.331
IPC_protein 8.244
Toseland 8.565
ProMoST 8.697
Dawson 9.019
Bjellqvist 9.033
Wikipedia 9.151
Rodwell 9.107
Grimsley 8.873
Solomon 9.121
Lehninger 9.077
Nozaki 9.092
DTASelect 8.902
Thurlkill 8.96
EMBOSS 9.151
Sillero 9.165
Patrickios 4.291
IPC_peptide 9.107
IPC2_peptide 7.849
IPC2.peptide.svr19 7.736
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
388
388
388
388.0
42.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.052 ± 0.0
1.546 ± 0.0
5.412 ± 0.0
3.608 ± 0.0
2.32 ± 0.0
5.928 ± 0.0
2.32 ± 0.0
4.124 ± 0.0
5.412 ± 0.0
6.701 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.32 ± 0.0
2.062 ± 0.0
9.278 ± 0.0
5.412 ± 0.0
5.67 ± 0.0
6.701 ± 0.0
6.701 ± 0.0
8.247 ± 0.0
1.546 ± 0.0
4.639 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here