Amino acid dipepetide frequency for Paenibacillus phage Rani

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.906AlaAla: 4.906 ± 1.097
0.423AlaCys: 0.423 ± 0.172
4.483AlaAsp: 4.483 ± 0.787
6.174AlaGlu: 6.174 ± 0.705
1.692AlaPhe: 1.692 ± 0.337
4.652AlaGly: 4.652 ± 0.807
1.015AlaHis: 1.015 ± 0.286
4.906AlaIle: 4.906 ± 0.88
5.582AlaLys: 5.582 ± 0.733
5.921AlaLeu: 5.921 ± 0.696
1.184AlaMet: 1.184 ± 0.312
3.129AlaAsn: 3.129 ± 0.481
1.015AlaPro: 1.015 ± 0.298
2.115AlaGln: 2.115 ± 0.395
2.622AlaArg: 2.622 ± 0.442
4.314AlaSer: 4.314 ± 0.666
3.891AlaThr: 3.891 ± 0.858
3.806AlaVal: 3.806 ± 0.478
1.1AlaTrp: 1.1 ± 0.316
2.537AlaTyr: 2.537 ± 0.475
0.0AlaXaa: 0.0 ± 0.0
Cys
0.507CysAla: 0.507 ± 0.24
0.254CysCys: 0.254 ± 0.127
0.338CysAsp: 0.338 ± 0.198
0.507CysGlu: 0.507 ± 0.202
0.169CysPhe: 0.169 ± 0.111
0.677CysGly: 0.677 ± 0.216
0.254CysHis: 0.254 ± 0.13
0.423CysIle: 0.423 ± 0.189
1.1CysLys: 1.1 ± 0.322
0.846CysLeu: 0.846 ± 0.299
0.254CysMet: 0.254 ± 0.138
0.338CysAsn: 0.338 ± 0.141
0.592CysPro: 0.592 ± 0.27
0.254CysGln: 0.254 ± 0.122
0.507CysArg: 0.507 ± 0.199
0.592CysSer: 0.592 ± 0.177
0.254CysThr: 0.254 ± 0.153
0.507CysVal: 0.507 ± 0.177
0.085CysTrp: 0.085 ± 0.077
0.254CysTyr: 0.254 ± 0.138
0.0CysXaa: 0.0 ± 0.0
Asp
3.806AspAla: 3.806 ± 0.737
0.423AspCys: 0.423 ± 0.168
3.891AspAsp: 3.891 ± 0.724
3.975AspGlu: 3.975 ± 0.638
2.876AspPhe: 2.876 ± 0.521
3.806AspGly: 3.806 ± 0.476
0.761AspHis: 0.761 ± 0.235
3.637AspIle: 3.637 ± 0.422
4.06AspLys: 4.06 ± 0.597
5.329AspLeu: 5.329 ± 0.574
2.03AspMet: 2.03 ± 0.399
1.692AspAsn: 1.692 ± 0.349
1.861AspPro: 1.861 ± 0.38
2.199AspGln: 2.199 ± 0.467
2.96AspArg: 2.96 ± 0.477
2.537AspSer: 2.537 ± 0.411
3.129AspThr: 3.129 ± 0.587
3.975AspVal: 3.975 ± 0.626
0.93AspTrp: 0.93 ± 0.28
2.284AspTyr: 2.284 ± 0.491
0.0AspXaa: 0.0 ± 0.0
Glu
5.244GluAla: 5.244 ± 0.622
0.338GluCys: 0.338 ± 0.163
4.821GluAsp: 4.821 ± 0.79
5.329GluGlu: 5.329 ± 0.749
2.707GluPhe: 2.707 ± 0.406
3.552GluGly: 3.552 ± 0.419
1.184GluHis: 1.184 ± 0.377
6.005GluIle: 6.005 ± 0.805
7.697GluLys: 7.697 ± 0.793
6.936GluLeu: 6.936 ± 0.793
4.821GluMet: 4.821 ± 0.521
3.637GluAsn: 3.637 ± 0.647
2.115GluPro: 2.115 ± 0.384
4.398GluGln: 4.398 ± 0.693
4.06GluArg: 4.06 ± 0.855
4.483GluSer: 4.483 ± 0.613
4.906GluThr: 4.906 ± 0.57
5.329GluVal: 5.329 ± 0.728
1.1GluTrp: 1.1 ± 0.292
3.045GluTyr: 3.045 ± 0.513
0.0GluXaa: 0.0 ± 0.0
Phe
1.861PheAla: 1.861 ± 0.327
0.592PheCys: 0.592 ± 0.194
2.115PheAsp: 2.115 ± 0.431
3.468PheGlu: 3.468 ± 0.582
1.1PhePhe: 1.1 ± 0.314
2.368PheGly: 2.368 ± 0.423
1.015PheHis: 1.015 ± 0.349
3.045PheIle: 3.045 ± 0.382
2.876PheLys: 2.876 ± 0.511
3.383PheLeu: 3.383 ± 0.575
1.015PheMet: 1.015 ± 0.284
1.861PheAsn: 1.861 ± 0.417
1.015PhePro: 1.015 ± 0.324
1.438PheGln: 1.438 ± 0.313
0.93PheArg: 0.93 ± 0.294
2.876PheSer: 2.876 ± 0.522
1.692PheThr: 1.692 ± 0.324
2.453PheVal: 2.453 ± 0.489
0.507PheTrp: 0.507 ± 0.181
1.184PheTyr: 1.184 ± 0.335
0.0PheXaa: 0.0 ± 0.0
Gly
3.129GlyAla: 3.129 ± 0.603
0.507GlyCys: 0.507 ± 0.167
2.791GlyAsp: 2.791 ± 0.6
4.99GlyGlu: 4.99 ± 0.769
2.791GlyPhe: 2.791 ± 0.555
3.045GlyGly: 3.045 ± 0.533
1.184GlyHis: 1.184 ± 0.376
5.752GlyIle: 5.752 ± 1.0
7.105GlyLys: 7.105 ± 0.621
4.906GlyLeu: 4.906 ± 0.873
1.776GlyMet: 1.776 ± 0.634
2.876GlyAsn: 2.876 ± 0.495
0.592GlyPro: 0.592 ± 0.274
2.453GlyGln: 2.453 ± 0.596
3.129GlyArg: 3.129 ± 0.529
3.552GlySer: 3.552 ± 0.859
2.791GlyThr: 2.791 ± 0.605
3.806GlyVal: 3.806 ± 0.527
0.93GlyTrp: 0.93 ± 0.195
2.791GlyTyr: 2.791 ± 0.513
0.0GlyXaa: 0.0 ± 0.0
His
1.1HisAla: 1.1 ± 0.265
0.254HisCys: 0.254 ± 0.146
0.761HisAsp: 0.761 ± 0.316
1.353HisGlu: 1.353 ± 0.426
0.761HisPhe: 0.761 ± 0.25
0.423HisGly: 0.423 ± 0.159
0.507HisHis: 0.507 ± 0.194
1.861HisIle: 1.861 ± 0.426
1.184HisLys: 1.184 ± 0.339
1.776HisLeu: 1.776 ± 0.389
0.338HisMet: 0.338 ± 0.158
0.846HisAsn: 0.846 ± 0.266
0.592HisPro: 0.592 ± 0.212
0.677HisGln: 0.677 ± 0.25
1.184HisArg: 1.184 ± 0.286
1.438HisSer: 1.438 ± 0.326
0.507HisThr: 0.507 ± 0.229
1.353HisVal: 1.353 ± 0.36
0.254HisTrp: 0.254 ± 0.136
0.423HisTyr: 0.423 ± 0.167
0.0HisXaa: 0.0 ± 0.0
Ile
4.144IleAla: 4.144 ± 0.696
0.761IleCys: 0.761 ± 0.247
4.229IleAsp: 4.229 ± 0.664
6.682IleGlu: 6.682 ± 0.723
2.876IlePhe: 2.876 ± 0.453
3.891IleGly: 3.891 ± 0.684
1.269IleHis: 1.269 ± 0.309
3.214IleIle: 3.214 ± 0.692
6.851IleLys: 6.851 ± 0.915
5.498IleLeu: 5.498 ± 0.682
1.353IleMet: 1.353 ± 0.353
3.383IleAsn: 3.383 ± 0.463
3.383IlePro: 3.383 ± 0.48
3.045IleGln: 3.045 ± 0.466
3.552IleArg: 3.552 ± 0.607
3.637IleSer: 3.637 ± 0.531
3.891IleThr: 3.891 ± 0.491
4.652IleVal: 4.652 ± 0.696
0.846IleTrp: 0.846 ± 0.296
2.876IleTyr: 2.876 ± 0.461
0.0IleXaa: 0.0 ± 0.0
Lys
5.921LysAla: 5.921 ± 0.655
0.761LysCys: 0.761 ± 0.228
4.398LysAsp: 4.398 ± 0.558
9.135LysGlu: 9.135 ± 0.891
2.199LysPhe: 2.199 ± 0.302
5.752LysGly: 5.752 ± 0.649
1.1LysHis: 1.1 ± 0.2
5.921LysIle: 5.921 ± 0.555
8.374LysLys: 8.374 ± 1.22
6.174LysLeu: 6.174 ± 0.665
3.637LysMet: 3.637 ± 0.531
4.314LysAsn: 4.314 ± 0.666
2.537LysPro: 2.537 ± 0.454
4.737LysGln: 4.737 ± 0.604
5.159LysArg: 5.159 ± 0.736
5.244LysSer: 5.244 ± 0.737
5.244LysThr: 5.244 ± 0.713
3.891LysVal: 3.891 ± 0.545
1.438LysTrp: 1.438 ± 0.302
2.707LysTyr: 2.707 ± 0.429
0.0LysXaa: 0.0 ± 0.0
Leu
6.344LeuAla: 6.344 ± 0.711
0.846LeuCys: 0.846 ± 0.233
4.567LeuAsp: 4.567 ± 0.546
7.274LeuGlu: 7.274 ± 0.776
3.045LeuPhe: 3.045 ± 0.557
5.159LeuGly: 5.159 ± 0.602
1.945LeuHis: 1.945 ± 0.367
4.652LeuIle: 4.652 ± 0.513
6.259LeuLys: 6.259 ± 0.677
6.259LeuLeu: 6.259 ± 0.837
1.607LeuMet: 1.607 ± 0.317
3.975LeuAsn: 3.975 ± 0.555
3.129LeuPro: 3.129 ± 0.53
4.229LeuGln: 4.229 ± 0.602
3.383LeuArg: 3.383 ± 0.595
6.513LeuSer: 6.513 ± 0.667
5.836LeuThr: 5.836 ± 0.662
4.144LeuVal: 4.144 ± 0.514
1.1LeuTrp: 1.1 ± 0.415
2.707LeuTyr: 2.707 ± 0.449
0.0LeuXaa: 0.0 ± 0.0
Met
2.03MetAla: 2.03 ± 0.37
0.0MetCys: 0.0 ± 0.0
1.438MetAsp: 1.438 ± 0.303
2.537MetGlu: 2.537 ± 0.417
1.353MetPhe: 1.353 ± 0.282
1.353MetGly: 1.353 ± 0.308
0.169MetHis: 0.169 ± 0.146
2.284MetIle: 2.284 ± 0.431
3.045MetLys: 3.045 ± 0.599
2.707MetLeu: 2.707 ± 0.456
0.846MetMet: 0.846 ± 0.269
2.284MetAsn: 2.284 ± 0.432
1.184MetPro: 1.184 ± 0.324
1.438MetGln: 1.438 ± 0.25
1.015MetArg: 1.015 ± 0.292
2.199MetSer: 2.199 ± 0.312
1.353MetThr: 1.353 ± 0.315
1.1MetVal: 1.1 ± 0.367
0.169MetTrp: 0.169 ± 0.127
1.1MetTyr: 1.1 ± 0.272
0.0MetXaa: 0.0 ± 0.0
Asn
2.284AsnAla: 2.284 ± 0.417
0.592AsnCys: 0.592 ± 0.238
1.945AsnAsp: 1.945 ± 0.458
3.129AsnGlu: 3.129 ± 0.61
1.184AsnPhe: 1.184 ± 0.303
3.806AsnGly: 3.806 ± 0.675
1.015AsnHis: 1.015 ± 0.263
2.96AsnIle: 2.96 ± 0.518
3.552AsnLys: 3.552 ± 0.51
3.975AsnLeu: 3.975 ± 0.674
1.269AsnMet: 1.269 ± 0.527
2.284AsnAsn: 2.284 ± 0.555
2.707AsnPro: 2.707 ± 0.43
1.438AsnGln: 1.438 ± 0.342
3.637AsnArg: 3.637 ± 0.726
2.622AsnSer: 2.622 ± 0.462
2.199AsnThr: 2.199 ± 0.463
2.707AsnVal: 2.707 ± 0.411
0.846AsnTrp: 0.846 ± 0.231
1.438AsnTyr: 1.438 ± 0.281
0.0AsnXaa: 0.0 ± 0.0
Pro
2.368ProAla: 2.368 ± 0.364
0.169ProCys: 0.169 ± 0.123
1.607ProAsp: 1.607 ± 0.258
2.876ProGlu: 2.876 ± 0.716
1.1ProPhe: 1.1 ± 0.26
2.453ProGly: 2.453 ± 0.395
0.846ProHis: 0.846 ± 0.262
2.537ProIle: 2.537 ± 0.454
3.383ProLys: 3.383 ± 0.693
2.284ProLeu: 2.284 ± 0.363
0.761ProMet: 0.761 ± 0.248
1.776ProAsn: 1.776 ± 0.371
1.015ProPro: 1.015 ± 0.3
0.93ProGln: 0.93 ± 0.341
0.761ProArg: 0.761 ± 0.265
3.299ProSer: 3.299 ± 0.552
2.03ProThr: 2.03 ± 0.334
2.115ProVal: 2.115 ± 0.53
0.507ProTrp: 0.507 ± 0.191
1.015ProTyr: 1.015 ± 0.234
0.0ProXaa: 0.0 ± 0.0
Gln
3.552GlnAla: 3.552 ± 0.565
0.169GlnCys: 0.169 ± 0.125
2.115GlnAsp: 2.115 ± 0.354
3.891GlnGlu: 3.891 ± 0.621
1.692GlnPhe: 1.692 ± 0.305
2.791GlnGly: 2.791 ± 0.523
0.93GlnHis: 0.93 ± 0.229
2.453GlnIle: 2.453 ± 0.432
3.383GlnLys: 3.383 ± 0.558
3.975GlnLeu: 3.975 ± 0.678
1.692GlnMet: 1.692 ± 0.412
1.353GlnAsn: 1.353 ± 0.313
2.115GlnPro: 2.115 ± 0.495
2.284GlnGln: 2.284 ± 0.5
2.707GlnArg: 2.707 ± 0.462
2.368GlnSer: 2.368 ± 0.351
2.537GlnThr: 2.537 ± 0.412
1.945GlnVal: 1.945 ± 0.398
0.507GlnTrp: 0.507 ± 0.226
1.269GlnTyr: 1.269 ± 0.327
0.0GlnXaa: 0.0 ± 0.0
Arg
2.622ArgAla: 2.622 ± 0.411
0.423ArgCys: 0.423 ± 0.2
2.96ArgAsp: 2.96 ± 0.458
3.637ArgGlu: 3.637 ± 0.628
1.522ArgPhe: 1.522 ± 0.38
2.622ArgGly: 2.622 ± 0.486
0.761ArgHis: 0.761 ± 0.214
3.045ArgIle: 3.045 ± 0.546
5.413ArgLys: 5.413 ± 0.847
4.398ArgLeu: 4.398 ± 0.68
1.438ArgMet: 1.438 ± 0.34
2.199ArgAsn: 2.199 ± 0.439
1.522ArgPro: 1.522 ± 0.36
2.537ArgGln: 2.537 ± 0.418
2.199ArgArg: 2.199 ± 0.402
2.115ArgSer: 2.115 ± 0.367
2.368ArgThr: 2.368 ± 0.532
2.791ArgVal: 2.791 ± 0.516
0.507ArgTrp: 0.507 ± 0.217
1.776ArgTyr: 1.776 ± 0.316
0.0ArgXaa: 0.0 ± 0.0
Ser
5.413SerAla: 5.413 ± 1.142
0.592SerCys: 0.592 ± 0.216
3.891SerAsp: 3.891 ± 0.554
5.244SerGlu: 5.244 ± 0.632
2.791SerPhe: 2.791 ± 0.437
4.06SerGly: 4.06 ± 0.678
1.184SerHis: 1.184 ± 0.401
6.09SerIle: 6.09 ± 0.87
4.06SerLys: 4.06 ± 0.612
5.582SerLeu: 5.582 ± 0.917
1.522SerMet: 1.522 ± 0.317
2.368SerAsn: 2.368 ± 0.372
2.368SerPro: 2.368 ± 0.417
2.876SerGln: 2.876 ± 0.498
2.368SerArg: 2.368 ± 0.416
3.891SerSer: 3.891 ± 0.646
3.637SerThr: 3.637 ± 0.615
4.144SerVal: 4.144 ± 0.878
0.423SerTrp: 0.423 ± 0.165
1.945SerTyr: 1.945 ± 0.308
0.0SerXaa: 0.0 ± 0.0
Thr
3.975ThrAla: 3.975 ± 0.716
0.338ThrCys: 0.338 ± 0.173
2.199ThrAsp: 2.199 ± 0.433
3.552ThrGlu: 3.552 ± 0.468
1.861ThrPhe: 1.861 ± 0.379
4.567ThrGly: 4.567 ± 0.537
1.1ThrHis: 1.1 ± 0.293
4.144ThrIle: 4.144 ± 0.628
5.329ThrLys: 5.329 ± 0.736
4.229ThrLeu: 4.229 ± 0.566
1.438ThrMet: 1.438 ± 0.443
1.776ThrAsn: 1.776 ± 0.344
2.791ThrPro: 2.791 ± 0.56
2.537ThrGln: 2.537 ± 0.463
2.199ThrArg: 2.199 ± 0.386
4.229ThrSer: 4.229 ± 0.636
2.537ThrThr: 2.537 ± 0.49
4.906ThrVal: 4.906 ± 0.608
0.423ThrTrp: 0.423 ± 0.182
2.453ThrTyr: 2.453 ± 0.465
0.0ThrXaa: 0.0 ± 0.0
Val
3.552ValAla: 3.552 ± 0.575
0.677ValCys: 0.677 ± 0.203
3.891ValAsp: 3.891 ± 0.63
4.737ValGlu: 4.737 ± 0.647
2.876ValPhe: 2.876 ± 0.49
3.214ValGly: 3.214 ± 0.553
0.507ValHis: 0.507 ± 0.252
4.06ValIle: 4.06 ± 0.558
5.075ValLys: 5.075 ± 0.67
4.398ValLeu: 4.398 ± 0.527
1.438ValMet: 1.438 ± 0.362
2.368ValAsn: 2.368 ± 0.425
1.776ValPro: 1.776 ± 0.375
2.03ValGln: 2.03 ± 0.362
2.453ValArg: 2.453 ± 0.479
5.075ValSer: 5.075 ± 0.574
4.99ValThr: 4.99 ± 0.592
3.552ValVal: 3.552 ± 0.458
1.776ValTrp: 1.776 ± 0.985
2.115ValTyr: 2.115 ± 0.495
0.0ValXaa: 0.0 ± 0.0
Trp
0.677TrpAla: 0.677 ± 0.209
0.169TrpCys: 0.169 ± 0.127
0.761TrpAsp: 0.761 ± 0.364
0.761TrpGlu: 0.761 ± 0.207
1.015TrpPhe: 1.015 ± 0.23
0.93TrpGly: 0.93 ± 0.282
0.423TrpHis: 0.423 ± 0.144
0.507TrpIle: 0.507 ± 0.177
1.015TrpLys: 1.015 ± 0.345
1.522TrpLeu: 1.522 ± 0.345
0.169TrpMet: 0.169 ± 0.111
1.776TrpAsn: 1.776 ± 1.168
0.338TrpPro: 0.338 ± 0.27
0.677TrpGln: 0.677 ± 0.236
0.338TrpArg: 0.338 ± 0.198
1.015TrpSer: 1.015 ± 0.243
0.507TrpThr: 0.507 ± 0.196
0.677TrpVal: 0.677 ± 0.269
0.169TrpTrp: 0.169 ± 0.098
0.423TrpTyr: 0.423 ± 0.193
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.03TyrAla: 2.03 ± 0.386
0.423TyrCys: 0.423 ± 0.204
2.96TyrAsp: 2.96 ± 0.517
2.453TyrGlu: 2.453 ± 0.438
1.184TyrPhe: 1.184 ± 0.281
1.776TyrGly: 1.776 ± 0.391
0.423TyrHis: 0.423 ± 0.193
2.537TyrIle: 2.537 ± 0.434
3.299TyrLys: 3.299 ± 0.488
2.876TyrLeu: 2.876 ± 0.46
0.93TyrMet: 0.93 ± 0.243
1.353TyrAsn: 1.353 ± 0.364
1.184TyrPro: 1.184 ± 0.298
1.438TyrGln: 1.438 ± 0.358
1.776TyrArg: 1.776 ± 0.413
2.453TyrSer: 2.453 ± 0.536
2.284TyrThr: 2.284 ± 0.394
2.707TyrVal: 2.707 ± 0.506
0.254TyrTrp: 0.254 ± 0.127
1.607TyrTyr: 1.607 ± 0.376
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 61 proteins (11824 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski