Sewage-associated gemycircularvirus 5

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemygorvirus; Gemygorvirus sewopo1; Sewage derived gemygorvirus 1

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A7CL64|A0A0A7CL64_9VIRU Capsid protein OS=Sewage-associated gemycircularvirus 5 OX=1519400 PE=4 SV=1
MM1 pKa = 7.68AEE3 pKa = 3.77SSFRR7 pKa = 11.84VQSRR11 pKa = 11.84YY12 pKa = 10.07VLLTYY17 pKa = 8.03SQCGEE22 pKa = 4.04LDD24 pKa = 2.95PWAVHH29 pKa = 7.07DD30 pKa = 5.8SITSYY35 pKa = 10.23PAEE38 pKa = 3.97CLIGRR43 pKa = 11.84EE44 pKa = 3.92NHH46 pKa = 6.51ADD48 pKa = 3.55GGIHH52 pKa = 6.42LHH54 pKa = 6.48AFVDD58 pKa = 4.58FGRR61 pKa = 11.84RR62 pKa = 11.84INLRR66 pKa = 11.84DD67 pKa = 3.19SRR69 pKa = 11.84RR70 pKa = 11.84FDD72 pKa = 3.29VEE74 pKa = 4.77GYY76 pKa = 9.96HH77 pKa = 7.3PNIQPCGRR85 pKa = 11.84TPQKK89 pKa = 10.33MLDD92 pKa = 3.61YY93 pKa = 10.33AIKK96 pKa = 10.91DD97 pKa = 3.49GDD99 pKa = 4.03VVAGGLSPTVGTEE112 pKa = 3.71VQGADD117 pKa = 3.52NVWARR122 pKa = 11.84IADD125 pKa = 3.77AASVEE130 pKa = 4.52EE131 pKa = 4.31FWEE134 pKa = 4.38LARR137 pKa = 11.84EE138 pKa = 4.16LAPRR142 pKa = 11.84ALLCNHH148 pKa = 5.94QSLRR152 pKa = 11.84AYY154 pKa = 10.25AEE156 pKa = 3.52WHH158 pKa = 5.67YY159 pKa = 11.35RR160 pKa = 11.84PATVAYY166 pKa = 6.72EE167 pKa = 4.18HH168 pKa = 7.23PPGLHH173 pKa = 6.47LCTGGVPEE181 pKa = 4.5LDD183 pKa = 2.61AWVRR187 pKa = 11.84DD188 pKa = 3.56NLLILWGEE196 pKa = 4.02TRR198 pKa = 11.84LGKK201 pKa = 7.89TVWARR206 pKa = 11.84SLGPHH211 pKa = 6.51VYY213 pKa = 10.27CCLQWNVDD221 pKa = 3.76DD222 pKa = 5.57LKK224 pKa = 11.47AGLEE228 pKa = 4.03DD229 pKa = 3.24AKK231 pKa = 11.24YY232 pKa = 10.74AVLDD236 pKa = 4.77DD237 pKa = 3.68IQGNFQFFPAYY248 pKa = 9.6KK249 pKa = 9.96GWLGGQATFTVTDD262 pKa = 4.1KK263 pKa = 11.38YY264 pKa = 10.68RR265 pKa = 11.84GKK267 pKa = 7.72TTVQWGRR274 pKa = 11.84PTIWLMNDD282 pKa = 3.12DD283 pKa = 4.37PEE285 pKa = 4.6EE286 pKa = 4.26VGHH289 pKa = 7.26VDD291 pKa = 5.07LNWLRR296 pKa = 11.84GNCDD300 pKa = 3.38IIHH303 pKa = 6.0LTEE306 pKa = 4.29SLISSS311 pKa = 4.28

Molecular weight: 35.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A7CL64|A0A0A7CL64_9VIRU Capsid protein OS=Sewage-associated gemycircularvirus 5 OX=1519400 PE=4 SV=1
MM1 pKa = 7.4PRR3 pKa = 11.84RR4 pKa = 11.84YY5 pKa = 7.91TRR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84ARR13 pKa = 11.84TTRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84PAFRR22 pKa = 11.84KK23 pKa = 8.44ATTTRR28 pKa = 11.84GRR30 pKa = 11.84RR31 pKa = 11.84TRR33 pKa = 11.84SFARR37 pKa = 11.84TRR39 pKa = 11.84HH40 pKa = 4.01SRR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84ILNITSIKK52 pKa = 10.24KK53 pKa = 9.42HH54 pKa = 6.76DD55 pKa = 3.97NMLPAVRR62 pKa = 11.84TADD65 pKa = 2.95GGYY68 pKa = 9.26SQNPLTTTTGFTSLFMPTARR88 pKa = 11.84EE89 pKa = 3.86LAHH92 pKa = 5.68TAEE95 pKa = 4.92GEE97 pKa = 4.42STRR100 pKa = 11.84QRR102 pKa = 11.84QTTFSVGYY110 pKa = 9.58KK111 pKa = 9.78EE112 pKa = 4.63RR113 pKa = 11.84LQIDD117 pKa = 3.92VQGGGVWKK125 pKa = 9.58WRR127 pKa = 11.84RR128 pKa = 11.84LIFTYY133 pKa = 10.33KK134 pKa = 10.42GDD136 pKa = 3.85SLFDD140 pKa = 4.33GDD142 pKa = 4.59PTWTQPFWDD151 pKa = 4.78KK152 pKa = 10.94STDD155 pKa = 3.53PDD157 pKa = 3.8GVDD160 pKa = 3.32MVRR163 pKa = 11.84LIAQPTLDD171 pKa = 3.65QHH173 pKa = 6.57ALWRR177 pKa = 11.84RR178 pKa = 11.84ILWDD182 pKa = 3.48GEE184 pKa = 4.29EE185 pKa = 4.33GVDD188 pKa = 3.36WNSEE192 pKa = 3.7FTAKK196 pKa = 10.26VDD198 pKa = 3.51TSRR201 pKa = 11.84ITPRR205 pKa = 11.84YY206 pKa = 10.0DD207 pKa = 3.0RR208 pKa = 11.84TITLNPGNEE217 pKa = 4.22SGMSRR222 pKa = 11.84TFRR225 pKa = 11.84LWHH228 pKa = 5.54PTRR231 pKa = 11.84KK232 pKa = 10.33NIVYY236 pKa = 10.46DD237 pKa = 3.82DD238 pKa = 4.79DD239 pKa = 4.43EE240 pKa = 5.93FGGAPNGGGAYY251 pKa = 9.46TSVTGKK257 pKa = 9.83PGMGDD262 pKa = 3.73LYY264 pKa = 11.33VYY266 pKa = 10.13DD267 pKa = 3.37IVYY270 pKa = 10.54NAVPAAAGNQALVFNPEE287 pKa = 3.3GTYY290 pKa = 10.18YY291 pKa = 9.06WHH293 pKa = 6.98EE294 pKa = 4.02RR295 pKa = 3.5

Molecular weight: 33.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

801

195

311

267.0

30.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.115 ± 0.839

1.623 ± 0.783

6.991 ± 0.199

5.243 ± 0.722

3.246 ± 0.391

8.739 ± 0.038

3.371 ± 0.637

3.87 ± 0.152

2.497 ± 0.495

7.99 ± 1.308

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.373 ± 0.321

3.496 ± 0.136

5.243 ± 0.207

3.246 ± 0.147

9.114 ± 1.88

4.869 ± 0.239

7.491 ± 2.093

6.242 ± 0.718

3.246 ± 0.305

3.995 ± 0.067
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski