Amino acid dipepetide frequency for Enterococcus phage IME-EFm5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.401AlaAla: 2.401 ± 0.539
0.232AlaCys: 0.232 ± 0.135
2.478AlaAsp: 2.478 ± 0.578
3.64AlaGlu: 3.64 ± 0.499
2.013AlaPhe: 2.013 ± 0.433
3.485AlaGly: 3.485 ± 0.655
1.007AlaHis: 1.007 ± 0.318
5.731AlaIle: 5.731 ± 1.139
4.492AlaLys: 4.492 ± 0.55
4.646AlaLeu: 4.646 ± 0.806
2.246AlaMet: 2.246 ± 0.671
3.098AlaAsn: 3.098 ± 0.415
2.633AlaPro: 2.633 ± 0.61
2.71AlaGln: 2.71 ± 0.429
2.478AlaArg: 2.478 ± 0.454
3.64AlaSer: 3.64 ± 0.621
3.64AlaThr: 3.64 ± 0.588
3.795AlaVal: 3.795 ± 0.483
1.007AlaTrp: 1.007 ± 0.305
3.175AlaTyr: 3.175 ± 0.456
0.0AlaXaa: 0.0 ± 0.0
Cys
0.31CysAla: 0.31 ± 0.161
0.077CysCys: 0.077 ± 0.073
0.465CysAsp: 0.465 ± 0.19
0.542CysGlu: 0.542 ± 0.3
0.155CysPhe: 0.155 ± 0.155
0.62CysGly: 0.62 ± 0.206
0.155CysHis: 0.155 ± 0.123
0.31CysIle: 0.31 ± 0.156
0.387CysLys: 0.387 ± 0.199
0.387CysLeu: 0.387 ± 0.18
0.232CysMet: 0.232 ± 0.128
0.232CysAsn: 0.232 ± 0.209
0.077CysPro: 0.077 ± 0.075
0.077CysGln: 0.077 ± 0.092
0.232CysArg: 0.232 ± 0.14
0.232CysSer: 0.232 ± 0.142
0.232CysThr: 0.232 ± 0.189
0.387CysVal: 0.387 ± 0.202
0.077CysTrp: 0.077 ± 0.08
0.31CysTyr: 0.31 ± 0.153
0.0CysXaa: 0.0 ± 0.0
Asp
3.33AspAla: 3.33 ± 0.653
0.31AspCys: 0.31 ± 0.186
3.407AspAsp: 3.407 ± 0.705
4.724AspGlu: 4.724 ± 0.662
2.246AspPhe: 2.246 ± 0.367
4.956AspGly: 4.956 ± 1.112
0.852AspHis: 0.852 ± 0.256
4.337AspIle: 4.337 ± 0.67
5.576AspLys: 5.576 ± 0.695
5.266AspLeu: 5.266 ± 0.809
1.936AspMet: 1.936 ± 0.338
3.485AspAsn: 3.485 ± 0.509
2.556AspPro: 2.556 ± 0.424
0.774AspGln: 0.774 ± 0.344
2.401AspArg: 2.401 ± 0.328
2.865AspSer: 2.865 ± 0.498
3.717AspThr: 3.717 ± 0.503
4.104AspVal: 4.104 ± 0.588
0.852AspTrp: 0.852 ± 0.251
3.098AspTyr: 3.098 ± 0.55
0.0AspXaa: 0.0 ± 0.0
Glu
4.569GluAla: 4.569 ± 0.529
0.62GluCys: 0.62 ± 0.24
4.492GluAsp: 4.492 ± 0.748
8.286GluGlu: 8.286 ± 1.266
4.027GluPhe: 4.027 ± 0.474
3.795GluGly: 3.795 ± 0.544
1.162GluHis: 1.162 ± 0.246
4.879GluIle: 4.879 ± 0.751
6.35GluLys: 6.35 ± 0.85
9.293GluLeu: 9.293 ± 1.161
2.556GluMet: 2.556 ± 0.513
3.253GluAsn: 3.253 ± 0.471
2.246GluPro: 2.246 ± 0.459
2.865GluGln: 2.865 ± 0.576
2.556GluArg: 2.556 ± 0.458
3.485GluSer: 3.485 ± 0.608
4.956GluThr: 4.956 ± 0.695
4.956GluVal: 4.956 ± 0.679
0.929GluTrp: 0.929 ± 0.229
3.717GluTyr: 3.717 ± 0.592
0.0GluXaa: 0.0 ± 0.0
Phe
1.549PheAla: 1.549 ± 0.325
0.232PheCys: 0.232 ± 0.151
2.865PheAsp: 2.865 ± 0.534
3.795PheGlu: 3.795 ± 0.771
0.929PhePhe: 0.929 ± 0.305
2.788PheGly: 2.788 ± 0.438
0.774PheHis: 0.774 ± 0.336
2.788PheIle: 2.788 ± 0.403
3.64PheLys: 3.64 ± 0.728
2.246PheLeu: 2.246 ± 0.415
1.471PheMet: 1.471 ± 0.306
2.633PheAsn: 2.633 ± 0.649
0.852PhePro: 0.852 ± 0.213
1.549PheGln: 1.549 ± 0.37
1.704PheArg: 1.704 ± 0.278
2.71PheSer: 2.71 ± 0.402
3.562PheThr: 3.562 ± 0.668
3.407PheVal: 3.407 ± 0.593
0.31PheTrp: 0.31 ± 0.155
1.626PheTyr: 1.626 ± 0.335
0.0PheXaa: 0.0 ± 0.0
Gly
4.646GlyAla: 4.646 ± 0.936
0.232GlyCys: 0.232 ± 0.203
3.872GlyAsp: 3.872 ± 0.611
3.872GlyGlu: 3.872 ± 0.479
3.795GlyPhe: 3.795 ± 0.498
4.492GlyGly: 4.492 ± 0.868
0.62GlyHis: 0.62 ± 0.202
3.95GlyIle: 3.95 ± 0.774
6.35GlyLys: 6.35 ± 0.767
4.492GlyLeu: 4.492 ± 0.736
1.162GlyMet: 1.162 ± 0.306
4.259GlyAsn: 4.259 ± 0.731
0.31GlyPro: 0.31 ± 0.128
2.401GlyGln: 2.401 ± 0.537
2.478GlyArg: 2.478 ± 0.409
3.407GlySer: 3.407 ± 0.527
3.872GlyThr: 3.872 ± 0.621
4.879GlyVal: 4.879 ± 0.849
1.239GlyTrp: 1.239 ± 0.309
3.098GlyTyr: 3.098 ± 0.482
0.0GlyXaa: 0.0 ± 0.0
His
0.852HisAla: 0.852 ± 0.299
0.0HisCys: 0.0 ± 0.0
1.162HisAsp: 1.162 ± 0.377
0.542HisGlu: 0.542 ± 0.211
0.774HisPhe: 0.774 ± 0.251
1.084HisGly: 1.084 ± 0.31
0.542HisHis: 0.542 ± 0.207
1.084HisIle: 1.084 ± 0.257
1.549HisLys: 1.549 ± 0.422
0.62HisLeu: 0.62 ± 0.234
0.077HisMet: 0.077 ± 0.086
1.317HisAsn: 1.317 ± 0.301
0.387HisPro: 0.387 ± 0.155
0.774HisGln: 0.774 ± 0.224
0.542HisArg: 0.542 ± 0.214
0.697HisSer: 0.697 ± 0.262
1.162HisThr: 1.162 ± 0.288
0.774HisVal: 0.774 ± 0.242
0.31HisTrp: 0.31 ± 0.148
0.929HisTyr: 0.929 ± 0.226
0.0HisXaa: 0.0 ± 0.0
Ile
4.492IleAla: 4.492 ± 0.573
0.387IleCys: 0.387 ± 0.181
5.189IleAsp: 5.189 ± 0.69
5.653IleGlu: 5.653 ± 0.641
1.626IlePhe: 1.626 ± 0.392
4.027IleGly: 4.027 ± 0.498
0.852IleHis: 0.852 ± 0.29
5.266IleIle: 5.266 ± 0.607
5.808IleLys: 5.808 ± 0.809
4.182IleLeu: 4.182 ± 0.644
2.013IleMet: 2.013 ± 0.411
4.956IleAsn: 4.956 ± 0.434
2.556IlePro: 2.556 ± 0.505
2.943IleGln: 2.943 ± 0.471
2.091IleArg: 2.091 ± 0.369
3.485IleSer: 3.485 ± 0.608
4.104IleThr: 4.104 ± 0.605
3.098IleVal: 3.098 ± 0.494
0.542IleTrp: 0.542 ± 0.218
2.168IleTyr: 2.168 ± 0.477
0.0IleXaa: 0.0 ± 0.0
Lys
5.343LysAla: 5.343 ± 0.85
0.232LysCys: 0.232 ± 0.114
5.498LysAsp: 5.498 ± 0.555
8.519LysGlu: 8.519 ± 1.079
3.717LysPhe: 3.717 ± 0.365
4.646LysGly: 4.646 ± 0.675
1.162LysHis: 1.162 ± 0.3
5.189LysIle: 5.189 ± 0.563
6.892LysLys: 6.892 ± 0.932
7.976LysLeu: 7.976 ± 0.81
2.246LysMet: 2.246 ± 0.475
4.956LysAsn: 4.956 ± 0.766
2.788LysPro: 2.788 ± 0.466
3.64LysGln: 3.64 ± 0.655
3.562LysArg: 3.562 ± 0.503
3.872LysSer: 3.872 ± 0.417
5.266LysThr: 5.266 ± 0.674
5.266LysVal: 5.266 ± 0.669
1.084LysTrp: 1.084 ± 0.299
5.034LysTyr: 5.034 ± 0.547
0.0LysXaa: 0.0 ± 0.0
Leu
5.343LeuAla: 5.343 ± 0.781
0.697LeuCys: 0.697 ± 0.328
6.04LeuAsp: 6.04 ± 0.521
6.66LeuGlu: 6.66 ± 1.072
3.795LeuPhe: 3.795 ± 0.738
4.646LeuGly: 4.646 ± 0.795
0.697LeuHis: 0.697 ± 0.208
3.872LeuIle: 3.872 ± 0.775
8.131LeuLys: 8.131 ± 0.821
7.512LeuLeu: 7.512 ± 0.939
1.781LeuMet: 1.781 ± 0.394
5.576LeuAsn: 5.576 ± 0.56
3.562LeuPro: 3.562 ± 0.49
2.943LeuGln: 2.943 ± 0.433
2.246LeuArg: 2.246 ± 0.383
5.653LeuSer: 5.653 ± 0.543
5.731LeuThr: 5.731 ± 0.727
4.337LeuVal: 4.337 ± 0.6
0.697LeuTrp: 0.697 ± 0.27
3.33LeuTyr: 3.33 ± 0.619
0.0LeuXaa: 0.0 ± 0.0
Met
1.162MetAla: 1.162 ± 0.33
0.077MetCys: 0.077 ± 0.08
1.317MetAsp: 1.317 ± 0.336
1.549MetGlu: 1.549 ± 0.373
0.929MetPhe: 0.929 ± 0.286
1.007MetGly: 1.007 ± 0.233
0.465MetHis: 0.465 ± 0.22
2.246MetIle: 2.246 ± 0.573
3.872MetLys: 3.872 ± 0.559
2.323MetLeu: 2.323 ± 0.488
1.007MetMet: 1.007 ± 0.356
2.168MetAsn: 2.168 ± 0.51
0.62MetPro: 0.62 ± 0.233
0.929MetGln: 0.929 ± 0.28
1.317MetArg: 1.317 ± 0.339
1.239MetSer: 1.239 ± 0.347
2.091MetThr: 2.091 ± 0.322
1.084MetVal: 1.084 ± 0.283
0.232MetTrp: 0.232 ± 0.121
1.317MetTyr: 1.317 ± 0.411
0.0MetXaa: 0.0 ± 0.0
Asn
4.182AsnAla: 4.182 ± 0.836
0.387AsnCys: 0.387 ± 0.195
2.556AsnAsp: 2.556 ± 0.491
4.724AsnGlu: 4.724 ± 0.609
2.168AsnPhe: 2.168 ± 0.448
4.724AsnGly: 4.724 ± 0.756
0.852AsnHis: 0.852 ± 0.307
4.104AsnIle: 4.104 ± 0.616
5.576AsnLys: 5.576 ± 0.606
4.801AsnLeu: 4.801 ± 0.525
1.704AsnMet: 1.704 ± 0.346
3.407AsnAsn: 3.407 ± 0.63
2.788AsnPro: 2.788 ± 0.504
2.246AsnGln: 2.246 ± 0.401
2.788AsnArg: 2.788 ± 0.364
3.485AsnSer: 3.485 ± 0.419
4.027AsnThr: 4.027 ± 0.662
3.795AsnVal: 3.795 ± 0.583
1.239AsnTrp: 1.239 ± 0.283
3.33AsnTyr: 3.33 ± 0.501
0.0AsnXaa: 0.0 ± 0.0
Pro
1.859ProAla: 1.859 ± 0.448
0.155ProCys: 0.155 ± 0.102
2.013ProAsp: 2.013 ± 0.398
2.943ProGlu: 2.943 ± 0.565
1.781ProPhe: 1.781 ± 0.38
0.852ProGly: 0.852 ± 0.48
0.232ProHis: 0.232 ± 0.118
2.091ProIle: 2.091 ± 0.354
2.865ProLys: 2.865 ± 0.391
2.71ProLeu: 2.71 ± 0.452
0.387ProMet: 0.387 ± 0.14
2.091ProAsn: 2.091 ± 0.514
1.007ProPro: 1.007 ± 0.29
1.704ProGln: 1.704 ± 0.298
0.697ProArg: 0.697 ± 0.222
1.781ProSer: 1.781 ± 0.547
1.781ProThr: 1.781 ± 0.405
2.091ProVal: 2.091 ± 0.332
0.542ProTrp: 0.542 ± 0.204
2.168ProTyr: 2.168 ± 0.468
0.0ProXaa: 0.0 ± 0.0
Gln
2.943GlnAla: 2.943 ± 0.451
0.465GlnCys: 0.465 ± 0.204
2.013GlnAsp: 2.013 ± 0.327
2.091GlnGlu: 2.091 ± 0.411
1.549GlnPhe: 1.549 ± 0.382
1.859GlnGly: 1.859 ± 0.44
0.774GlnHis: 0.774 ± 0.25
1.781GlnIle: 1.781 ± 0.358
2.556GlnLys: 2.556 ± 0.436
3.717GlnLeu: 3.717 ± 0.669
1.394GlnMet: 1.394 ± 0.38
1.859GlnAsn: 1.859 ± 0.336
1.162GlnPro: 1.162 ± 0.423
1.317GlnGln: 1.317 ± 0.389
1.317GlnArg: 1.317 ± 0.315
2.401GlnSer: 2.401 ± 0.419
1.936GlnThr: 1.936 ± 0.321
2.71GlnVal: 2.71 ± 0.424
0.387GlnTrp: 0.387 ± 0.156
1.626GlnTyr: 1.626 ± 0.385
0.0GlnXaa: 0.0 ± 0.0
Arg
2.013ArgAla: 2.013 ± 0.4
0.155ArgCys: 0.155 ± 0.174
2.865ArgAsp: 2.865 ± 0.515
2.788ArgGlu: 2.788 ± 0.555
1.781ArgPhe: 1.781 ± 0.398
2.556ArgGly: 2.556 ± 0.347
1.084ArgHis: 1.084 ± 0.276
1.781ArgIle: 1.781 ± 0.399
2.556ArgLys: 2.556 ± 0.616
3.253ArgLeu: 3.253 ± 0.549
1.394ArgMet: 1.394 ± 0.303
1.626ArgAsn: 1.626 ± 0.334
0.774ArgPro: 0.774 ± 0.236
1.239ArgGln: 1.239 ± 0.312
1.626ArgArg: 1.626 ± 0.326
2.323ArgSer: 2.323 ± 0.318
2.323ArgThr: 2.323 ± 0.493
2.168ArgVal: 2.168 ± 0.393
0.387ArgTrp: 0.387 ± 0.169
2.168ArgTyr: 2.168 ± 0.423
0.0ArgXaa: 0.0 ± 0.0
Ser
3.02SerAla: 3.02 ± 0.566
0.232SerCys: 0.232 ± 0.142
2.788SerAsp: 2.788 ± 0.438
3.485SerGlu: 3.485 ± 0.475
2.478SerPhe: 2.478 ± 0.511
5.111SerGly: 5.111 ± 1.031
1.007SerHis: 1.007 ± 0.279
3.098SerIle: 3.098 ± 0.41
5.034SerLys: 5.034 ± 0.844
4.182SerLeu: 4.182 ± 0.567
1.239SerMet: 1.239 ± 0.328
3.795SerAsn: 3.795 ± 0.684
1.471SerPro: 1.471 ± 0.283
2.091SerGln: 2.091 ± 0.474
1.859SerArg: 1.859 ± 0.47
2.788SerSer: 2.788 ± 0.481
4.337SerThr: 4.337 ± 0.932
3.33SerVal: 3.33 ± 0.689
0.852SerTrp: 0.852 ± 0.365
2.323SerTyr: 2.323 ± 0.512
0.0SerXaa: 0.0 ± 0.0
Thr
3.02ThrAla: 3.02 ± 0.615
0.077ThrCys: 0.077 ± 0.088
4.027ThrAsp: 4.027 ± 0.679
6.04ThrGlu: 6.04 ± 0.596
2.865ThrPhe: 2.865 ± 0.528
4.879ThrGly: 4.879 ± 0.748
1.239ThrHis: 1.239 ± 0.355
3.795ThrIle: 3.795 ± 0.613
5.886ThrLys: 5.886 ± 0.675
5.498ThrLeu: 5.498 ± 0.628
0.929ThrMet: 0.929 ± 0.287
4.646ThrAsn: 4.646 ± 0.548
2.865ThrPro: 2.865 ± 0.566
2.323ThrGln: 2.323 ± 0.485
1.936ThrArg: 1.936 ± 0.468
3.02ThrSer: 3.02 ± 0.646
5.343ThrThr: 5.343 ± 1.048
4.801ThrVal: 4.801 ± 0.612
0.774ThrTrp: 0.774 ± 0.251
3.02ThrTyr: 3.02 ± 0.565
0.0ThrXaa: 0.0 ± 0.0
Val
4.182ValAla: 4.182 ± 0.769
0.387ValCys: 0.387 ± 0.165
4.104ValAsp: 4.104 ± 0.578
4.724ValGlu: 4.724 ± 0.57
2.401ValPhe: 2.401 ± 0.491
5.343ValGly: 5.343 ± 0.723
0.62ValHis: 0.62 ± 0.193
3.95ValIle: 3.95 ± 0.526
4.879ValLys: 4.879 ± 0.504
4.724ValLeu: 4.724 ± 0.536
1.394ValMet: 1.394 ± 0.45
5.498ValAsn: 5.498 ± 0.683
1.781ValPro: 1.781 ± 0.342
1.471ValGln: 1.471 ± 0.399
2.788ValArg: 2.788 ± 0.55
3.872ValSer: 3.872 ± 0.553
3.717ValThr: 3.717 ± 0.469
4.414ValVal: 4.414 ± 0.732
0.929ValTrp: 0.929 ± 0.299
2.401ValTyr: 2.401 ± 0.421
0.0ValXaa: 0.0 ± 0.0
Trp
0.62TrpAla: 0.62 ± 0.205
0.0TrpCys: 0.0 ± 0.0
0.852TrpAsp: 0.852 ± 0.249
0.852TrpGlu: 0.852 ± 0.255
0.62TrpPhe: 0.62 ± 0.226
0.387TrpGly: 0.387 ± 0.188
0.232TrpHis: 0.232 ± 0.14
0.929TrpIle: 0.929 ± 0.324
0.542TrpLys: 0.542 ± 0.254
1.704TrpLeu: 1.704 ± 0.36
0.387TrpMet: 0.387 ± 0.147
0.697TrpAsn: 0.697 ± 0.337
0.077TrpPro: 0.077 ± 0.068
0.465TrpGln: 0.465 ± 0.2
0.697TrpArg: 0.697 ± 0.196
0.852TrpSer: 0.852 ± 0.255
1.317TrpThr: 1.317 ± 0.487
0.929TrpVal: 0.929 ± 0.223
0.155TrpTrp: 0.155 ± 0.111
0.62TrpTyr: 0.62 ± 0.227
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.71TyrAla: 2.71 ± 0.476
0.542TyrCys: 0.542 ± 0.253
2.788TyrAsp: 2.788 ± 0.394
3.64TyrGlu: 3.64 ± 0.584
1.781TyrPhe: 1.781 ± 0.416
2.168TyrGly: 2.168 ± 0.324
0.852TyrHis: 0.852 ± 0.288
4.259TyrIle: 4.259 ± 0.569
3.95TyrLys: 3.95 ± 0.655
3.717TyrLeu: 3.717 ± 0.544
1.317TyrMet: 1.317 ± 0.293
3.33TyrAsn: 3.33 ± 0.586
1.239TyrPro: 1.239 ± 0.297
1.394TyrGln: 1.394 ± 0.352
1.549TyrArg: 1.549 ± 0.282
2.71TyrSer: 2.71 ± 0.417
3.872TyrThr: 3.872 ± 0.632
3.253TyrVal: 3.253 ± 0.578
0.387TyrTrp: 0.387 ± 0.199
2.788TyrTyr: 2.788 ± 0.698
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 70 proteins (12914 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski