Okra leaf curl virus-[Cameroon]

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; unclassified Begomovirus; Okra leaf curl virus

Average proteome isoelectric point is 7.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C5J4Q8|C5J4Q8_9GEMI Uncharacterized protein OS=Okra leaf curl virus-[Cameroon] OX=627503 GN=C4 PE=3 SV=1
MM1 pKa = 7.64APNKK5 pKa = 10.05RR6 pKa = 11.84FQIYY10 pKa = 10.34AKK12 pKa = 10.52NFFLTFPKK20 pKa = 10.45CSLSKK25 pKa = 11.15EE26 pKa = 4.04EE27 pKa = 5.44ALEE30 pKa = 3.78QLQQISTASNKK41 pKa = 10.08KK42 pKa = 9.09YY43 pKa = 10.71IKK45 pKa = 9.68ICRR48 pKa = 11.84EE49 pKa = 3.81LHH51 pKa = 6.97EE52 pKa = 5.88DD53 pKa = 3.95GQPHH57 pKa = 6.66LHH59 pKa = 6.38VLLQFEE65 pKa = 5.47GKK67 pKa = 10.02FKK69 pKa = 10.87CQNQRR74 pKa = 11.84LFDD77 pKa = 4.01LVSPNRR83 pKa = 11.84SAHH86 pKa = 5.06FHH88 pKa = 6.56PNIQGAKK95 pKa = 9.25SSSDD99 pKa = 3.11VKK101 pKa = 11.24SYY103 pKa = 10.79IDD105 pKa = 3.7KK106 pKa = 11.33DD107 pKa = 3.49GDD109 pKa = 3.67TLEE112 pKa = 4.14WGEE115 pKa = 3.84FQIDD119 pKa = 3.21GRR121 pKa = 11.84SARR124 pKa = 11.84GGQQTANDD132 pKa = 4.05AYY134 pKa = 10.69AAALNAGSKK143 pKa = 9.22TEE145 pKa = 3.75ALRR148 pKa = 11.84VIRR151 pKa = 11.84EE152 pKa = 4.09LAPKK156 pKa = 10.51DD157 pKa = 4.02FVLQYY162 pKa = 11.26HH163 pKa = 5.78NLINNLDD170 pKa = 4.39RR171 pKa = 11.84IFQEE175 pKa = 4.24PPAPYY180 pKa = 9.99VSPFLSSSFDD190 pKa = 3.44QVPEE194 pKa = 3.98EE195 pKa = 4.13LEE197 pKa = 3.58EE198 pKa = 3.9WAAEE202 pKa = 4.15NVVEE206 pKa = 4.32AAARR210 pKa = 11.84PGRR213 pKa = 11.84PISIVIEE220 pKa = 4.35GEE222 pKa = 3.93SRR224 pKa = 11.84TGKK227 pKa = 7.7TVWARR232 pKa = 11.84SLGPHH237 pKa = 6.71NYY239 pKa = 10.18LCGHH243 pKa = 7.38LDD245 pKa = 3.88LSPKK249 pKa = 10.04VFSNDD254 pKa = 1.85AWYY257 pKa = 10.93NVIDD261 pKa = 5.2DD262 pKa = 4.41VDD264 pKa = 3.61PHH266 pKa = 5.85YY267 pKa = 11.0LKK269 pKa = 10.7HH270 pKa = 6.2FKK272 pKa = 10.76EE273 pKa = 4.49FMGAQKK279 pKa = 10.58DD280 pKa = 3.71WQSNTKK286 pKa = 9.54YY287 pKa = 10.57GKK289 pKa = 9.12PVQIKK294 pKa = 10.41GGIPTIFLCNPGPNSSYY311 pKa = 11.09KK312 pKa = 10.65DD313 pKa = 3.59YY314 pKa = 11.45LDD316 pKa = 3.85EE317 pKa = 4.94EE318 pKa = 4.85KK319 pKa = 10.74NAHH322 pKa = 5.77LKK324 pKa = 9.97SWALKK329 pKa = 8.81NATFITLSYY338 pKa = 10.0PLYY341 pKa = 10.72SGTNQSPASGGQEE354 pKa = 3.63EE355 pKa = 5.28SNQEE359 pKa = 3.99TQDD362 pKa = 3.25

Molecular weight: 40.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C5J4Q6|C5J4Q6_9GEMI Transcriptional activator protein OS=Okra leaf curl virus-[Cameroon] OX=627503 PE=3 SV=1
MM1 pKa = 8.27DD2 pKa = 4.36SRR4 pKa = 11.84TGEE7 pKa = 5.1LITAHH12 pKa = 4.42QTEE15 pKa = 4.56NGVLIWTINNPLYY28 pKa = 10.71FKK30 pKa = 9.77TIKK33 pKa = 10.11EE34 pKa = 3.98IPLTHH39 pKa = 6.84GNQTMVEE46 pKa = 3.86MQIRR50 pKa = 11.84FNYY53 pKa = 9.94NLRR56 pKa = 11.84KK57 pKa = 9.59EE58 pKa = 4.28LGIHH62 pKa = 5.36KK63 pKa = 9.9CFMNFRR69 pKa = 11.84VWTISRR75 pKa = 11.84PPTGLFLNVFRR86 pKa = 11.84KK87 pKa = 10.15QIMKK91 pKa = 9.64YY92 pKa = 10.0LYY94 pKa = 8.72RR95 pKa = 11.84LGVISINNVIRR106 pKa = 11.84AVNHH110 pKa = 5.07VLYY113 pKa = 10.55DD114 pKa = 3.56VLQTTVASEE123 pKa = 4.24FTHH126 pKa = 6.83NIQIKK131 pKa = 10.32LYY133 pKa = 10.05

Molecular weight: 15.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

902

85

362

150.3

17.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.208 ± 1.109

2.106 ± 0.534

4.878 ± 0.98

5.765 ± 0.652

4.213 ± 0.536

5.1 ± 0.588

3.659 ± 0.494

6.208 ± 0.983

5.322 ± 0.953

8.093 ± 0.921

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.328 ± 0.849

5.987 ± 0.643

5.322 ± 0.588

5.654 ± 0.725

6.319 ± 1.455

7.539 ± 1.402

5.765 ± 1.256

4.545 ± 0.774

1.33 ± 0.224

3.659 ± 0.638
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski