Gammapapillomavirus 9
Average proteome isoelectric point is 6.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2D2ALP6|A0A2D2ALP6_9PAPI Major capsid protein L1 OS=Gammapapillomavirus 9 OX=1175851 GN=L1 PE=3 SV=1
MM1 pKa = 6.99 NRR3 pKa = 11.84 SRR5 pKa = 11.84 RR6 pKa = 11.84 KK7 pKa = 9.06 RR8 pKa = 11.84 ASPTEE13 pKa = 4.19 LYY15 pKa = 10.22 KK16 pKa = 10.83 SCAMGGDD23 pKa = 4.97 CIPDD27 pKa = 3.29 VKK29 pKa = 11.11 NKK31 pKa = 10.15 IEE33 pKa = 4.16 GTTLADD39 pKa = 2.99 ILLKK43 pKa = 11.03 VFGSVIYY50 pKa = 10.49 LGNLGIGTGRR60 pKa = 11.84 GSGGQYY66 pKa = 10.53 GYY68 pKa = 9.21 TPFGGTKK75 pKa = 8.31 PTTSATPLRR84 pKa = 11.84 PSIPTDD90 pKa = 3.47 TVVTGDD96 pKa = 3.92 ILPVTPYY103 pKa = 11.05 DD104 pKa = 3.69 SAIVPLTDD112 pKa = 4.25 GLPEE116 pKa = 3.87 PAIIDD121 pKa = 3.92 TPGVGPGLPTDD132 pKa = 3.76 SVTVTTTIDD141 pKa = 4.22 PISEE145 pKa = 4.03 VTGVGEE151 pKa = 4.39 HH152 pKa = 6.97 PNVIYY157 pKa = 10.94 NADD160 pKa = 3.38 NVAQLDD166 pKa = 4.17 VQLQPPPPKK175 pKa = 10.16 KK176 pKa = 10.68 VILDD180 pKa = 3.63 ASIHH184 pKa = 4.58 NTEE187 pKa = 4.46 TNIHH191 pKa = 4.7 THH193 pKa = 6.3 ASHH196 pKa = 6.8 VDD198 pKa = 2.75 SDD200 pKa = 4.09 YY201 pKa = 11.48 TVFVDD206 pKa = 3.89 AQFHH210 pKa = 6.4 GEE212 pKa = 4.04 HH213 pKa = 6.65 IGPVEE218 pKa = 4.21 EE219 pKa = 6.17 IEE221 pKa = 4.15 LQSINLRR228 pKa = 11.84 EE229 pKa = 3.97 EE230 pKa = 4.09 FEE232 pKa = 4.12 IDD234 pKa = 3.22 EE235 pKa = 5.19 GPLKK239 pKa = 10.0 STPLSSRR246 pKa = 11.84 LINRR250 pKa = 11.84 TRR252 pKa = 11.84 DD253 pKa = 3.09 LYY255 pKa = 11.18 HH256 pKa = 6.95 RR257 pKa = 11.84 FVEE260 pKa = 4.31 QVPTQKK266 pKa = 10.52 EE267 pKa = 4.45 VVLSRR272 pKa = 11.84 PGVTYY277 pKa = 10.4 EE278 pKa = 4.16 FEE280 pKa = 4.5 NPAFDD285 pKa = 5.1 PDD287 pKa = 4.09 VIDD290 pKa = 3.46 VFEE293 pKa = 5.3 RR294 pKa = 11.84 EE295 pKa = 4.21 VQEE298 pKa = 4.44 VARR301 pKa = 11.84 TAPLEE306 pKa = 4.16 DD307 pKa = 3.66 TQDD310 pKa = 3.71 VIRR313 pKa = 11.84 LGDD316 pKa = 3.45 TRR318 pKa = 11.84 FDD320 pKa = 3.42 EE321 pKa = 4.72 SPRR324 pKa = 11.84 GTVRR328 pKa = 11.84 VSRR331 pKa = 11.84 LGQRR335 pKa = 11.84 SGMITRR341 pKa = 11.84 SGLQIGKK348 pKa = 9.33 RR349 pKa = 11.84 VHH351 pKa = 6.69 FYY353 pKa = 11.36 YY354 pKa = 10.5 DD355 pKa = 3.04 VSPIAGEE362 pKa = 4.26 AIEE365 pKa = 4.32 LRR367 pKa = 11.84 TVGEE371 pKa = 4.23 YY372 pKa = 10.01 SHH374 pKa = 6.86 EE375 pKa = 4.17 SSIVDD380 pKa = 3.62 EE381 pKa = 4.64 LTTSSFVNPFEE392 pKa = 4.16 MPIDD396 pKa = 3.9 SSLQFSDD403 pKa = 5.57 DD404 pKa = 3.66 MLLEE408 pKa = 4.35 SATEE412 pKa = 4.06 DD413 pKa = 3.61 FSNSHH418 pKa = 6.84 VILTNTEE425 pKa = 3.81 TDD427 pKa = 3.11 GDD429 pKa = 4.51 AIDD432 pKa = 4.78 IPVNLPNEE440 pKa = 4.29 GVKK443 pKa = 10.59 VFVDD447 pKa = 5.67 DD448 pKa = 3.57 YY449 pKa = 9.16 TQPLFVSHH457 pKa = 7.78 PINVDD462 pKa = 3.03 TSVIDD467 pKa = 3.77 FDD469 pKa = 4.19 TPLIPFQPSFGLDD482 pKa = 3.2 VNYY485 pKa = 10.14 FDD487 pKa = 5.84 YY488 pKa = 11.07 DD489 pKa = 3.36 IHH491 pKa = 7.22 PSLLLRR497 pKa = 11.84 KK498 pKa = 9.26 RR499 pKa = 11.84 KK500 pKa = 9.87 YY501 pKa = 10.18 PVFFTLL507 pKa = 4.95
Molecular weight: 55.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.533
IPC2_protein 4.495
IPC_protein 4.469
Toseland 4.304
ProMoST 4.596
Dawson 4.444
Bjellqvist 4.584
Wikipedia 4.329
Rodwell 4.317
Grimsley 4.215
Solomon 4.444
Lehninger 4.393
Nozaki 4.546
DTASelect 4.749
Thurlkill 4.317
EMBOSS 4.342
Sillero 4.596
Patrickios 3.808
IPC_peptide 4.444
IPC2_peptide 4.584
IPC2.peptide.svr19 4.537
Protein with the highest isoelectric point:
>tr|A0A2D2ALI8|A0A2D2ALI8_9PAPI Protein E7 OS=Gammapapillomavirus 9 OX=1175851 GN=E7 PE=3 SV=1
MM1 pKa = 6.91 EE2 pKa = 4.86 TLRR5 pKa = 11.84 PTDD8 pKa = 3.62 LKK10 pKa = 11.06 SYY12 pKa = 8.9 CTIFGIDD19 pKa = 3.71 FFEE22 pKa = 5.15 LHH24 pKa = 6.27 LQCIFCNCYY33 pKa = 10.42 LSLVDD38 pKa = 5.81 LADD41 pKa = 5.18 FYY43 pKa = 10.63 TKK45 pKa = 10.29 CLCLVWRR52 pKa = 11.84 VGKK55 pKa = 10.13 AHH57 pKa = 7.09 ACCAKK62 pKa = 10.16 CLCLSAKK69 pKa = 10.42 CEE71 pKa = 4.23 LEE73 pKa = 4.04 RR74 pKa = 11.84 HH75 pKa = 4.99 VQCSAKK81 pKa = 10.18 VVNLHH86 pKa = 6.34 SLTNKK91 pKa = 9.15 PLCEE95 pKa = 3.94 LKK97 pKa = 10.77 VRR99 pKa = 11.84 CCQCLALLDD108 pKa = 4.17 LQEE111 pKa = 4.62 KK112 pKa = 9.9 CDD114 pKa = 3.71 LVARR118 pKa = 11.84 GKK120 pKa = 9.98 DD121 pKa = 3.19 AYY123 pKa = 10.58 LVRR126 pKa = 11.84 GNWRR130 pKa = 11.84 APCRR134 pKa = 11.84 KK135 pKa = 10.06 CIDD138 pKa = 3.53 RR139 pKa = 11.84 EE140 pKa = 4.05 II141 pKa = 5.35
Molecular weight: 16.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.167
IPC2_protein 7.322
IPC_protein 7.102
Toseland 6.576
ProMoST 7.746
Dawson 7.819
Bjellqvist 8.39
Wikipedia 7.717
Rodwell 7.834
Grimsley 6.576
Solomon 7.849
Lehninger 7.878
Nozaki 8.814
DTASelect 7.995
Thurlkill 8.039
EMBOSS 8.039
Sillero 8.448
Patrickios 1.1
IPC_peptide 7.849
IPC2_peptide 7.907
IPC2.peptide.svr19 7.696
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2377
98
597
339.6
38.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.721 ± 0.563
2.65 ± 0.928
7.068 ± 0.477
6.142 ± 0.535
4.544 ± 0.507
5.09 ± 0.725
2.188 ± 0.345
4.754 ± 0.794
5.469 ± 0.677
9.382 ± 0.546
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.43 ± 0.241
4.88 ± 0.392
6.31 ± 1.029
4.249 ± 0.591
5.848 ± 0.706
7.404 ± 0.449
5.89 ± 0.675
6.479 ± 0.787
1.262 ± 0.366
3.239 ± 0.333
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here