Serratia phage MTx
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 103 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A482MGP0|A0A482MGP0_9CAUD Uncharacterized protein OS=Serratia phage MTx OX=2557553 GN=CPT_MTx_046 PE=4 SV=1
MM1 pKa = 7.79 AFTIEE6 pKa = 4.31 SIKK9 pKa = 10.51 AAKK12 pKa = 9.57 FADD15 pKa = 3.65 AGCYY19 pKa = 7.3 VTLEE23 pKa = 4.47 SALEE27 pKa = 4.01 TYY29 pKa = 10.31 IGVKK33 pKa = 7.06 VTRR36 pKa = 11.84 EE37 pKa = 3.76 LSEE40 pKa = 4.64 GEE42 pKa = 3.49 FDD44 pKa = 5.39 AIFAEE49 pKa = 4.61 FFEE52 pKa = 5.41 DD53 pKa = 3.41 EE54 pKa = 4.35 GRR56 pKa = 11.84 DD57 pKa = 3.65 TSSDD61 pKa = 3.06 GSLEE65 pKa = 4.0 IDD67 pKa = 3.02 GVEE70 pKa = 3.93 YY71 pKa = 11.15 NFAIDD76 pKa = 4.41 LGRR79 pKa = 11.84 KK80 pKa = 9.1 CFIIFYY86 pKa = 9.24 EE87 pKa = 4.45 IYY89 pKa = 10.98 VKK91 pKa = 10.79 DD92 pKa = 3.85 EE93 pKa = 4.29 NGNDD97 pKa = 3.53 TDD99 pKa = 4.19 EE100 pKa = 4.63 VDD102 pKa = 3.74 LFSAKK107 pKa = 9.98 GFAIEE112 pKa = 4.18 EE113 pKa = 4.32 LL114 pKa = 3.87
Molecular weight: 12.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.132
IPC2_protein 3.935
IPC_protein 3.872
Toseland 3.681
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.732
Rodwell 3.706
Grimsley 3.605
Solomon 3.821
Lehninger 3.783
Nozaki 3.948
DTASelect 4.101
Thurlkill 3.719
EMBOSS 3.745
Sillero 3.986
Patrickios 3.274
IPC_peptide 3.834
IPC2_peptide 3.973
IPC2.peptide.svr19 3.906
Protein with the highest isoelectric point:
>tr|A0A482MGN9|A0A482MGN9_9CAUD Uncharacterized protein OS=Serratia phage MTx OX=2557553 GN=CPT_MTx_004 PE=4 SV=1
MM1 pKa = 7.35 LVLRR5 pKa = 11.84 RR6 pKa = 11.84 AIFSYY11 pKa = 10.38 RR12 pKa = 11.84 RR13 pKa = 11.84 AAWEE17 pKa = 3.98 SEE19 pKa = 3.66 EE20 pKa = 4.12 RR21 pKa = 11.84 APEE24 pKa = 4.04 AKK26 pKa = 10.33 ASASVRR32 pKa = 11.84 FVGPAANLVSMPIHH46 pKa = 6.18 FVV48 pKa = 3.09
Molecular weight: 5.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.327
IPC2_protein 9.648
IPC_protein 10.833
Toseland 10.818
ProMoST 10.906
Dawson 10.891
Bjellqvist 10.73
Wikipedia 11.228
Rodwell 10.804
Grimsley 10.95
Solomon 11.184
Lehninger 11.125
Nozaki 10.789
DTASelect 10.745
Thurlkill 10.818
EMBOSS 11.257
Sillero 10.847
Patrickios 10.716
IPC_peptide 11.184
IPC2_peptide 9.94
IPC2.peptide.svr19 8.696
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
103
0
103
21163
48
959
205.5
22.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.304 ± 0.302
1.172 ± 0.092
6.218 ± 0.172
6.091 ± 0.399
4.201 ± 0.13
7.267 ± 0.246
1.753 ± 0.143
6.389 ± 0.233
6.237 ± 0.317
7.263 ± 0.209
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.996 ± 0.129
4.943 ± 0.221
3.931 ± 0.179
3.894 ± 0.21
4.99 ± 0.188
6.077 ± 0.244
5.76 ± 0.284
6.752 ± 0.185
1.384 ± 0.079
3.379 ± 0.152
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here