Escherichia phage aaroes

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Tempevirinae; Hanrivervirus; Escherichia virus aaroes

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9WM41|A0A6B9WM41_9CAUD Putative membrane associated protein OS=Escherichia phage aaroes OX=2696376 GN=aaroes_67 PE=4 SV=1
MM1 pKa = 7.74SDD3 pKa = 3.22YY4 pKa = 11.28SVIVGAEE11 pKa = 4.1SPGCMVDD18 pKa = 3.27SSIMVINGSFVCGDD32 pKa = 3.5EE33 pKa = 4.85FISPGKK39 pKa = 9.77IVRR42 pKa = 11.84LSYY45 pKa = 10.45ISSGYY50 pKa = 10.37KK51 pKa = 9.2IVSVNGDD58 pKa = 3.68LVIGVAVRR66 pKa = 11.84PPDD69 pKa = 3.35SCIYY73 pKa = 10.81EE74 pKa = 4.15EE75 pKa = 4.69GDD77 pKa = 3.41PVNVVSRR84 pKa = 11.84GSVWCLTSEE93 pKa = 4.54SEE95 pKa = 4.24APEE98 pKa = 4.21YY99 pKa = 10.36GDD101 pKa = 4.61KK102 pKa = 11.37VFVTQDD108 pKa = 2.18GDD110 pKa = 3.34AAYY113 pKa = 10.76SEE115 pKa = 5.59GYY117 pKa = 9.96LLSGWIFTGEE127 pKa = 4.12HH128 pKa = 5.33VKK130 pKa = 10.26VDD132 pKa = 3.4HH133 pKa = 7.37DD134 pKa = 4.49SYY136 pKa = 11.45IVGVSISAA144 pKa = 3.44

Molecular weight: 15.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9WPJ1|A0A6B9WPJ1_9CAUD Terminase small subunit OS=Escherichia phage aaroes OX=2696376 GN=aaroes_45 PE=4 SV=1
MM1 pKa = 7.85LEE3 pKa = 3.87LNYY6 pKa = 10.0IRR8 pKa = 11.84VGTKK12 pKa = 9.78FRR14 pKa = 11.84VTWVYY19 pKa = 11.02DD20 pKa = 3.64EE21 pKa = 4.54RR22 pKa = 11.84CGIRR26 pKa = 11.84KK27 pKa = 9.94GEE29 pKa = 4.08IVTASDD35 pKa = 4.04RR36 pKa = 11.84YY37 pKa = 9.95IGSLKK42 pKa = 10.18EE43 pKa = 3.88FNRR46 pKa = 11.84PRR48 pKa = 11.84IKK50 pKa = 10.36NGYY53 pKa = 9.12IITRR57 pKa = 11.84RR58 pKa = 11.84LGFDD62 pKa = 3.72HH63 pKa = 6.74YY64 pKa = 11.16CVVATQGVLIEE75 pKa = 4.44LKK77 pKa = 10.31RR78 pKa = 11.84ISDD81 pKa = 3.56HH82 pKa = 6.68RR83 pKa = 11.84GCHH86 pKa = 4.72VKK88 pKa = 9.37TMKK91 pKa = 10.19MPFQRR96 pKa = 11.84ARR98 pKa = 11.84YY99 pKa = 7.81DD100 pKa = 3.15ARR102 pKa = 11.84RR103 pKa = 11.84MRR105 pKa = 11.84RR106 pKa = 11.84LARR109 pKa = 11.84NAIKK113 pKa = 10.23FKK115 pKa = 11.11KK116 pKa = 9.32PGNDD120 pKa = 3.09FYY122 pKa = 12.26VMYY125 pKa = 10.6KK126 pKa = 10.58GIARR130 pKa = 11.84NAGKK134 pKa = 10.28

Molecular weight: 15.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

82

0

82

15369

50

1192

187.4

21.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.198 ± 0.431

1.464 ± 0.154

5.999 ± 0.255

6.676 ± 0.25

3.923 ± 0.21

7.47 ± 0.242

1.932 ± 0.207

6.559 ± 0.197

7.437 ± 0.308

6.442 ± 0.196

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.201 ± 0.21

4.73 ± 0.206

3.325 ± 0.199

3.813 ± 0.293

5.069 ± 0.221

6.207 ± 0.298

5.498 ± 0.274

6.851 ± 0.192

1.412 ± 0.101

3.793 ± 0.152
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski