Streptococcus phage phi-SsuFJZZ32_rum

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G3MKN8|A0A7G3MKN8_9CAUD Uncharacterized protein OS=Streptococcus phage phi-SsuFJZZ32_rum OX=2664396 PE=4 SV=1
MM1 pKa = 7.55NDD3 pKa = 4.25EE4 pKa = 4.47INLDD8 pKa = 4.15KK9 pKa = 10.9IVLTAIEE16 pKa = 4.56TEE18 pKa = 4.53MKK20 pKa = 10.02CKK22 pKa = 10.42NYY24 pKa = 10.72LMLKK28 pKa = 9.49SHH30 pKa = 7.39GIDD33 pKa = 3.32IISDD37 pKa = 3.63YY38 pKa = 11.14YY39 pKa = 11.4YY40 pKa = 10.68VDD42 pKa = 3.32EE43 pKa = 5.2DD44 pKa = 3.92FAVITFSRR52 pKa = 11.84NEE54 pKa = 3.94DD55 pKa = 3.07KK56 pKa = 9.03MTIRR60 pKa = 11.84VEE62 pKa = 3.96NQEE65 pKa = 4.84CIVCVEE71 pKa = 3.93NHH73 pKa = 4.89VVKK76 pKa = 10.85AFEE79 pKa = 4.3SFLEE83 pKa = 4.65GEE85 pKa = 5.13DD86 pKa = 3.59IDD88 pKa = 4.27DD89 pKa = 4.71QIEE92 pKa = 4.0ILIKK96 pKa = 9.26QLCIGKK102 pKa = 8.17WQIFIEE108 pKa = 4.4DD109 pKa = 3.6VANNSLVDD117 pKa = 4.85AIQKK121 pKa = 10.32HH122 pKa = 5.36GFDD125 pKa = 5.13DD126 pKa = 4.3YY127 pKa = 11.97SNGPDD132 pKa = 3.45AIIYY136 pKa = 7.4FF137 pKa = 4.24

Molecular weight: 15.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G3MKJ3|A0A7G3MKJ3_9CAUD Head head-tail preconnector protease C OS=Streptococcus phage phi-SsuFJZZ32_rum OX=2664396 PE=3 SV=1
MM1 pKa = 7.74PRR3 pKa = 11.84RR4 pKa = 11.84PSTPCKK10 pKa = 10.41QNGCPNLVPYY20 pKa = 8.51GQKK23 pKa = 9.9YY24 pKa = 8.86CANHH28 pKa = 6.26KK29 pKa = 10.31VNHH32 pKa = 5.75QLDD35 pKa = 3.96AKK37 pKa = 8.2STKK40 pKa = 10.13AKK42 pKa = 10.15GYY44 pKa = 9.42NGQWTKK50 pKa = 11.25ARR52 pKa = 11.84LRR54 pKa = 11.84YY55 pKa = 10.0LKK57 pKa = 9.81VHH59 pKa = 6.46PLCVQCKK66 pKa = 9.75AKK68 pKa = 10.52GRR70 pKa = 11.84LTKK73 pKa = 10.1ATVVDD78 pKa = 5.28HH79 pKa = 6.23ITPHH83 pKa = 6.97RR84 pKa = 11.84GDD86 pKa = 5.06QEE88 pKa = 5.0LFWNQTNWQALCKK101 pKa = 9.93SCHH104 pKa = 5.64DD105 pKa = 4.22RR106 pKa = 11.84KK107 pKa = 8.93TKK109 pKa = 8.13TTDD112 pKa = 2.58RR113 pKa = 11.84YY114 pKa = 10.6VEE116 pKa = 3.73YY117 pKa = 9.41TYY119 pKa = 11.18RR120 pKa = 11.84FF121 pKa = 3.55

Molecular weight: 14.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

22191

39

1515

280.9

31.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.034 ± 0.661

0.816 ± 0.114

5.795 ± 0.227

7.809 ± 0.308

4.006 ± 0.248

5.768 ± 0.234

1.766 ± 0.114

6.823 ± 0.304

7.674 ± 0.243

9.067 ± 0.221

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.217 ± 0.153

5.408 ± 0.191

2.771 ± 0.15

4.538 ± 0.229

4.592 ± 0.182

6.624 ± 0.261

6.147 ± 0.434

6.102 ± 0.169

1.176 ± 0.104

3.866 ± 0.262
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski