Streptococcus gordonii (strain Challis / ATCC 35105 / BCRC 15272 / CH1 / DL1 / V288)
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2050 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A8AWL4|A8AWL4_STRGC ABC transporter ATP-binding protein OS=Streptococcus gordonii (strain Challis / ATCC 35105 / BCRC 15272 / CH1 / DL1 / V288) OX=467705 GN=SGO_0878 PE=4 SV=1
MM1 pKa = 7.54 LSAIEE6 pKa = 4.3 AQDD9 pKa = 4.05 LVQAEE14 pKa = 4.18 QWFEE18 pKa = 3.8 KK19 pKa = 10.93 ALLEE23 pKa = 4.89 DD24 pKa = 4.02 SDD26 pKa = 6.15 SILLDD31 pKa = 3.36 LAEE34 pKa = 4.27 YY35 pKa = 10.55 LEE37 pKa = 4.9 SIGFFLQAKK46 pKa = 9.75 RR47 pKa = 11.84 IYY49 pKa = 10.68 NKK51 pKa = 10.12 LAPLYY56 pKa = 9.5 PQVNLNLAAIASEE69 pKa = 4.67 DD70 pKa = 3.66 GDD72 pKa = 3.91 LEE74 pKa = 4.2 EE75 pKa = 4.84 AFGYY79 pKa = 10.63 LEE81 pKa = 4.98 EE82 pKa = 5.09 IRR84 pKa = 11.84 SNDD87 pKa = 2.84 PWYY90 pKa = 11.48 VNALLIKK97 pKa = 10.25 ADD99 pKa = 4.48 LYY101 pKa = 11.42 QMEE104 pKa = 4.82 GLTDD108 pKa = 3.38 VARR111 pKa = 11.84 EE112 pKa = 3.98 KK113 pKa = 10.85 LVEE116 pKa = 3.98 ASQLSDD122 pKa = 3.88 DD123 pKa = 4.87 PIITFGMAEE132 pKa = 3.96 IDD134 pKa = 3.8 FEE136 pKa = 5.22 LGNFTQAIKK145 pKa = 10.43 EE146 pKa = 4.12 YY147 pKa = 10.81 ASLDD151 pKa = 3.12 NRR153 pKa = 11.84 TIYY156 pKa = 10.09 EE157 pKa = 3.78 QTGISTYY164 pKa = 8.97 QRR166 pKa = 11.84 IGVSYY171 pKa = 10.58 ASLGKK176 pKa = 10.23 FEE178 pKa = 4.47 AAIEE182 pKa = 3.97 FLQKK186 pKa = 10.61 AIEE189 pKa = 4.07 LEE191 pKa = 4.09 YY192 pKa = 11.23 DD193 pKa = 3.24 EE194 pKa = 5.59 SAVFEE199 pKa = 4.48 LAVILYY205 pKa = 9.62 DD206 pKa = 4.08 QEE208 pKa = 5.67 DD209 pKa = 4.02 YY210 pKa = 11.49 QKK212 pKa = 11.61 ANLYY216 pKa = 9.62 FKK218 pKa = 10.66 QLDD221 pKa = 3.78 TLSPDD226 pKa = 3.33 FEE228 pKa = 5.45 GYY230 pKa = 10.42 EE231 pKa = 4.02 YY232 pKa = 10.09 IYY234 pKa = 11.05 ALSLHH239 pKa = 7.05 ADD241 pKa = 3.63 NQAEE245 pKa = 4.1 AALLIAQQGLNKK257 pKa = 10.36 NPFDD261 pKa = 3.81 SQLALLASQVSYY273 pKa = 10.66 EE274 pKa = 3.98 LHH276 pKa = 6.08 DD277 pKa = 3.74 TEE279 pKa = 5.15 KK280 pKa = 11.23 AEE282 pKa = 5.3 SYY284 pKa = 11.31 LLDD287 pKa = 3.57 AWDD290 pKa = 4.04 NADD293 pKa = 4.81 DD294 pKa = 4.7 LEE296 pKa = 4.24 EE297 pKa = 4.05 LALRR301 pKa = 11.84 LTNLYY306 pKa = 10.37 LEE308 pKa = 4.28 QEE310 pKa = 4.23 RR311 pKa = 11.84 YY312 pKa = 9.88 EE313 pKa = 5.19 DD314 pKa = 3.7 ILALEE319 pKa = 4.59 DD320 pKa = 3.87 QDD322 pKa = 3.26 WDD324 pKa = 3.77 NVLTRR329 pKa = 11.84 WNIARR334 pKa = 11.84 SYY336 pKa = 10.33 QALEE340 pKa = 4.94 DD341 pKa = 3.59 IEE343 pKa = 4.64 KK344 pKa = 10.56 AQEE347 pKa = 4.09 LYY349 pKa = 11.41 GEE351 pKa = 4.28 LHH353 pKa = 6.96 RR354 pKa = 11.84 DD355 pKa = 3.25 LRR357 pKa = 11.84 DD358 pKa = 3.34 NPEE361 pKa = 3.68 FLEE364 pKa = 4.32 EE365 pKa = 4.04 YY366 pKa = 10.05 IYY368 pKa = 10.97 LLRR371 pKa = 11.84 EE372 pKa = 4.19 CGEE375 pKa = 4.31 LEE377 pKa = 4.22 SAKK380 pKa = 10.47 QEE382 pKa = 4.08 AQHH385 pKa = 5.92 YY386 pKa = 9.59 LSLVPDD392 pKa = 4.65 DD393 pKa = 4.68 SAMQEE398 pKa = 4.58 FYY400 pKa = 11.35 DD401 pKa = 4.11 SLDD404 pKa = 3.53 YY405 pKa = 11.4
Molecular weight: 46.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.739
IPC2_protein 3.91
IPC_protein 3.897
Toseland 3.706
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.745
Rodwell 3.719
Grimsley 3.605
Solomon 3.859
Lehninger 3.808
Nozaki 3.961
DTASelect 4.139
Thurlkill 3.732
EMBOSS 3.757
Sillero 4.012
Patrickios 1.176
IPC_peptide 3.859
IPC2_peptide 3.999
IPC2.peptide.svr19 3.891
Protein with the highest isoelectric point:
>tr|A8AUQ7|A8AUQ7_STRGC Small ribosomal subunit biogenesis GTPase RsgA OS=Streptococcus gordonii (strain Challis / ATCC 35105 / BCRC 15272 / CH1 / DL1 / V288) OX=467705 GN=rsgA PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.07 QPSKK9 pKa = 9.18 IRR11 pKa = 11.84 RR12 pKa = 11.84 ARR14 pKa = 11.84 KK15 pKa = 8.35 HH16 pKa = 4.78 GFRR19 pKa = 11.84 NRR21 pKa = 11.84 MSTKK25 pKa = 9.22 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.87 GRR39 pKa = 11.84 KK40 pKa = 8.75 VLAAA44 pKa = 4.31
Molecular weight: 5.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.449
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.135
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.082
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2050
0
2050
642221
30
3646
313.3
35.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.668 ± 0.067
0.526 ± 0.016
5.646 ± 0.049
7.196 ± 0.061
4.62 ± 0.054
6.564 ± 0.056
1.83 ± 0.022
7.067 ± 0.074
6.868 ± 0.061
10.223 ± 0.105
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.356 ± 0.029
4.356 ± 0.043
3.424 ± 0.052
4.202 ± 0.046
3.968 ± 0.041
6.377 ± 0.109
5.533 ± 0.081
6.875 ± 0.049
0.905 ± 0.018
3.797 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here