Mixia osmundae (strain CBS 9802 / IAM 14324 / JCM 22182 / KY 12970)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Pucciniomycotina; Mixiomycetes; Mixiales; Mixiaceae; Mixia; Mixia osmundae

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6725 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G7E4A3|G7E4A3_MIXOS Cytochrome b5 heme-binding domain-containing protein OS=Mixia osmundae (strain CBS 9802 / IAM 14324 / JCM 22182 / KY 12970) OX=764103 GN=Mo04341 PE=4 SV=1
MM1 pKa = 7.66KK2 pKa = 9.53LTMLMLMTLALLAAARR18 pKa = 11.84PQDD21 pKa = 4.3DD22 pKa = 5.02GGDD25 pKa = 3.71DD26 pKa = 6.16DD27 pKa = 5.63YY28 pKa = 12.13DD29 pKa = 5.99CDD31 pKa = 5.85DD32 pKa = 5.08FDD34 pKa = 4.3NQADD38 pKa = 3.91CRR40 pKa = 11.84AHH42 pKa = 7.15SDD44 pKa = 4.19CKK46 pKa = 9.55WFPYY50 pKa = 10.09GCEE53 pKa = 3.68

Molecular weight: 5.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G7E9S1|G7E9S1_MIXOS Uncharacterized protein OS=Mixia osmundae (strain CBS 9802 / IAM 14324 / JCM 22182 / KY 12970) OX=764103 GN=Mo06087 PE=4 SV=1
MM1 pKa = 7.36QSALLDD7 pKa = 3.94SVLLLSVPALICWRR21 pKa = 11.84PRR23 pKa = 11.84RR24 pKa = 11.84TSSSEE29 pKa = 3.57GGIMDD34 pKa = 4.8SLLDD38 pKa = 3.74FAGNALSFVLLILATVLFIGFVTGSVMLFEE68 pKa = 5.87RR69 pKa = 11.84ISAYY73 pKa = 10.53VSGPAPVVTNRR84 pKa = 11.84NRR86 pKa = 11.84ATDD89 pKa = 3.65RR90 pKa = 11.84AQHH93 pKa = 4.72HH94 pKa = 5.84TEE96 pKa = 4.28RR97 pKa = 11.84ISVVVITIMLTLSFWWFPALFNAFNDD123 pKa = 3.67WLQLRR128 pKa = 11.84PRR130 pKa = 11.84KK131 pKa = 9.87DD132 pKa = 3.29AVPVKK137 pKa = 10.12IIEE140 pKa = 4.27RR141 pKa = 11.84PVIRR145 pKa = 11.84QFRR148 pKa = 11.84SVLDD152 pKa = 3.83PCWNSSTGQIIISLGLMIVIVVYY175 pKa = 10.69ISHH178 pKa = 5.89SHH180 pKa = 5.98SGDD183 pKa = 3.03VAAKK187 pKa = 9.49RR188 pKa = 11.84KK189 pKa = 8.88PIPPPAQPFKK199 pKa = 10.88RR200 pKa = 11.84RR201 pKa = 11.84VDD203 pKa = 3.88LPPTFTQLADD213 pKa = 3.61PRR215 pKa = 11.84KK216 pKa = 8.87HH217 pKa = 5.77ARR219 pKa = 11.84TRR221 pKa = 11.84HH222 pKa = 3.99EE223 pKa = 4.15HH224 pKa = 4.67MHH226 pKa = 6.6LGAIFKK232 pKa = 10.74ASDD235 pKa = 3.29GQTTYY240 pKa = 10.63TNPSGPRR247 pKa = 11.84LLAFQATKK255 pKa = 10.49RR256 pKa = 11.84RR257 pKa = 11.84RR258 pKa = 11.84LFYY261 pKa = 10.6LRR263 pKa = 11.84PAQLSQAQRR272 pKa = 11.84ARR274 pKa = 11.84SGLLIHH280 pKa = 7.25DD281 pKa = 4.71LQWGCKK287 pKa = 6.74TAGCIHH293 pKa = 7.22RR294 pKa = 11.84KK295 pKa = 8.23QGVRR299 pKa = 11.84EE300 pKa = 3.89AHH302 pKa = 6.95KK303 pKa = 10.56ISTSPGWSHH312 pKa = 8.04DD313 pKa = 3.2IGQLGPSSGGSSMVCGCFEE332 pKa = 4.43GKK334 pKa = 10.26ASPVLATRR342 pKa = 11.84VTRR345 pKa = 11.84CYY347 pKa = 11.12

Molecular weight: 38.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6725

0

6725

3482161

9

5000

517.8

56.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.478 ± 0.027

1.213 ± 0.012

5.775 ± 0.017

5.488 ± 0.023

3.232 ± 0.016

6.156 ± 0.023

2.456 ± 0.013

4.534 ± 0.019

4.299 ± 0.024

9.551 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.996 ± 0.01

2.862 ± 0.013

5.976 ± 0.03

4.367 ± 0.02

6.74 ± 0.023

9.294 ± 0.045

6.091 ± 0.022

5.819 ± 0.019

1.182 ± 0.01

2.493 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski