Ruminococcus sp. CAG:57

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Ruminococcus; environmental samples

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2264 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6U6Q4|R6U6Q4_9FIRM RNA polymerase sigma factor SigS OS=Ruminococcus sp. CAG:57 OX=1262962 GN=BN714_01887 PE=3 SV=1
MM1 pKa = 8.29DD2 pKa = 4.44IFEE5 pKa = 4.89EE6 pKa = 4.64NCDD9 pKa = 3.68TEE11 pKa = 4.67NNCTVSYY18 pKa = 9.56FWKK21 pKa = 10.61GLALFLLGVIIGLFVSPIKK40 pKa = 10.64KK41 pKa = 10.15GVTIGCNNKK50 pKa = 8.98IDD52 pKa = 3.94KK53 pKa = 10.6GGCNDD58 pKa = 3.9HH59 pKa = 7.19FDD61 pKa = 3.88PDD63 pKa = 3.97EE64 pKa = 4.84YY65 pKa = 11.47YY66 pKa = 11.09DD67 pKa = 5.61DD68 pKa = 5.06YY69 pKa = 12.07NFDD72 pKa = 4.35DD73 pKa = 5.88DD74 pKa = 6.02EE75 pKa = 7.63DD76 pKa = 6.13DD77 pKa = 4.19FDD79 pKa = 6.21HH80 pKa = 7.78DD81 pKa = 4.12GVKK84 pKa = 10.35FF85 pKa = 3.55

Molecular weight: 9.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6TEW7|R6TEW7_9FIRM Fructose-1 6-bisphosphatase class 3 OS=Ruminococcus sp. CAG:57 OX=1262962 GN=fbp PE=3 SV=1
MM1 pKa = 6.89SQHH4 pKa = 5.2IRR6 pKa = 11.84VYY8 pKa = 10.98LSFKK12 pKa = 9.77LFCYY16 pKa = 10.14RR17 pKa = 11.84LCFRR21 pKa = 11.84GRR23 pKa = 11.84LIIQLIFCRR32 pKa = 11.84DD33 pKa = 3.37PYY35 pKa = 10.89FHH37 pKa = 6.85YY38 pKa = 10.44LAYY41 pKa = 10.94LMNIRR46 pKa = 11.84LVQRR50 pKa = 11.84HH51 pKa = 5.79EE52 pKa = 4.12PPAVIRR58 pKa = 11.84ICFHH62 pKa = 5.57VAHH65 pKa = 7.02VYY67 pKa = 10.9NFVKK71 pKa = 10.58PPVQACFQAEE81 pKa = 3.99QLHH84 pKa = 6.29IILRR88 pKa = 11.84IVFFVSRR95 pKa = 11.84NLQLYY100 pKa = 7.17EE101 pKa = 3.94QFFPRR106 pKa = 11.84LWKK109 pKa = 10.75SKK111 pKa = 9.92IIQLKK116 pKa = 10.28IPLLRR121 pKa = 11.84NKK123 pKa = 8.91CHH125 pKa = 6.64RR126 pKa = 11.84IYY128 pKa = 10.81RR129 pKa = 11.84RR130 pKa = 11.84IHH132 pKa = 6.07FFQLLFF138 pKa = 4.3

Molecular weight: 17.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2264

0

2264

701634

30

2961

309.9

34.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.513 ± 0.053

1.805 ± 0.024

6.44 ± 0.044

6.967 ± 0.053

4.246 ± 0.04

6.896 ± 0.052

1.566 ± 0.019

7.379 ± 0.045

7.683 ± 0.047

8.206 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.892 ± 0.026

4.688 ± 0.043

3.091 ± 0.031

2.657 ± 0.024

3.891 ± 0.043

6.377 ± 0.049

5.767 ± 0.046

6.968 ± 0.039

0.789 ± 0.018

4.178 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski