Paenibacillus phage Diva

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fernvirus; Paenibacillus virus Diva

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5AJ36|A0A0C5AJ36_9CAUD Beta-lactamase-like hydrolase OS=Paenibacillus phage Diva OX=1589750 GN=DIVA_44 PE=4 SV=1
MM1 pKa = 8.31IEE3 pKa = 3.96DD4 pKa = 3.88CRR6 pKa = 11.84AQEE9 pKa = 4.42TIPATAQSVYY19 pKa = 10.72DD20 pKa = 3.66QYY22 pKa = 12.02LKK24 pKa = 10.79VYY26 pKa = 8.85QQFINTEE33 pKa = 3.99DD34 pKa = 3.52SAEE37 pKa = 4.01AEE39 pKa = 4.26MLQEE43 pKa = 4.57DD44 pKa = 4.67LKK46 pKa = 11.75DD47 pKa = 3.44MEE49 pKa = 4.44RR50 pKa = 11.84KK51 pKa = 10.11YY52 pKa = 11.15GIGEE56 pKa = 3.92

Molecular weight: 6.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5AEK1|A0A0C5AEK1_9CAUD Transcriptional regulator OS=Paenibacillus phage Diva OX=1589750 GN=DIVA_35 PE=4 SV=1
MM1 pKa = 7.46KK2 pKa = 10.41FGVRR6 pKa = 11.84KK7 pKa = 9.37PSIKK11 pKa = 10.16KK12 pKa = 10.21SIAARR17 pKa = 11.84TSIKK21 pKa = 10.16RR22 pKa = 11.84QIVHH26 pKa = 6.6RR27 pKa = 11.84AGIKK31 pKa = 8.89MPRR34 pKa = 11.84GYY36 pKa = 10.97GAIRR40 pKa = 11.84NPKK43 pKa = 8.38KK44 pKa = 9.79AARR47 pKa = 11.84NKK49 pKa = 9.71VYY51 pKa = 11.1NKK53 pKa = 7.61TTVSFWGMIKK63 pKa = 10.45KK64 pKa = 10.01LFKK67 pKa = 10.83

Molecular weight: 7.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

11182

36

878

186.4

21.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.895 ± 0.431

0.885 ± 0.146

5.75 ± 0.254

8.147 ± 0.431

3.479 ± 0.201

6.18 ± 0.424

1.842 ± 0.196

6.904 ± 0.307

8.684 ± 0.356

8.084 ± 0.291

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.014 ± 0.192

4.579 ± 0.228

3.327 ± 0.21

4.284 ± 0.226

5.053 ± 0.389

6.376 ± 0.287

5.455 ± 0.255

5.983 ± 0.253

1.467 ± 0.201

3.613 ± 0.292
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski