Lactobacillus cacaonum DSM 21116
Average proteome isoelectric point is 6.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1801 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R2CJT3|A0A0R2CJT3_9LACO Ion Mg(2+) C(O2+) transport protein OS=Lactobacillus cacaonum DSM 21116 OX=1423729 GN=FC80_GL000079 PE=4 SV=1
MM1 pKa = 7.37 NLGSNPNGADD11 pKa = 3.65 GIGFAFHH18 pKa = 7.1 NGNTDD23 pKa = 3.97 DD24 pKa = 4.75 IGNSGANLGIGGLQNAIGFKK44 pKa = 10.62 LDD46 pKa = 3.08 TWKK49 pKa = 10.98 NGYY52 pKa = 7.3 QTPNKK57 pKa = 9.42 DD58 pKa = 3.19 VPGAQISTPDD68 pKa = 3.37 SNGFGWNGDD77 pKa = 3.82 SKK79 pKa = 9.98 GTPYY83 pKa = 10.1 GTFVTTSDD91 pKa = 3.62 QQIKK95 pKa = 7.23 TTDD98 pKa = 3.2 GTEE101 pKa = 3.99 VQRR104 pKa = 11.84 WWAVDD109 pKa = 3.02 SGTAQSLSKK118 pKa = 10.99 ADD120 pKa = 3.22 IDD122 pKa = 4.63 GNFHH126 pKa = 6.35 NFVVSYY132 pKa = 10.69 DD133 pKa = 3.2 GSTRR137 pKa = 11.84 TLTINYY143 pKa = 7.85 TEE145 pKa = 4.36 TDD147 pKa = 3.21 GTMLTWSMQVDD158 pKa = 3.85 DD159 pKa = 5.78 AYY161 pKa = 9.93 TAMSMIVSASTGSATNLQQFKK182 pKa = 10.26 IDD184 pKa = 3.51 SFDD187 pKa = 3.55 FQEE190 pKa = 4.19 AATVNVKK197 pKa = 10.5 YY198 pKa = 10.73 VDD200 pKa = 3.62 EE201 pKa = 4.89 NGNSIADD208 pKa = 3.95 GEE210 pKa = 4.62 VTYY213 pKa = 10.82 PDD215 pKa = 3.55 GPYY218 pKa = 11.03 VNGTYY223 pKa = 7.49 TTVQKK228 pKa = 10.57 DD229 pKa = 3.09 IPNYY233 pKa = 10.18 KK234 pKa = 10.01 FIGMGDD240 pKa = 3.36 NATTGVSIAKK250 pKa = 9.8 DD251 pKa = 3.17 GTMADD256 pKa = 4.08 DD257 pKa = 4.56 GNNGTVIYY265 pKa = 9.71 VYY267 pKa = 10.88 APAYY271 pKa = 10.06 SATSKK276 pKa = 9.9 TINEE280 pKa = 4.46 TINYY284 pKa = 8.98 VDD286 pKa = 3.37 QNGNKK291 pKa = 9.47 VADD294 pKa = 4.45 SYY296 pKa = 10.95 TATPITVVTVTNPVDD311 pKa = 3.59 GSKK314 pKa = 9.54 TYY316 pKa = 10.31 YY317 pKa = 10.02 YY318 pKa = 11.02 KK319 pKa = 10.78 SGTDD323 pKa = 3.46 DD324 pKa = 4.68 TPTLDD329 pKa = 3.51 NNGVPEE335 pKa = 4.39 GEE337 pKa = 4.37 GWTKK341 pKa = 11.14 ADD343 pKa = 3.65 SSDD346 pKa = 3.18 FTAVKK351 pKa = 10.55 NPTVDD356 pKa = 3.42 GYY358 pKa = 11.73 KK359 pKa = 10.35 VISNDD364 pKa = 3.78 APNSDD369 pKa = 3.82 LTQVADD375 pKa = 3.57 QTITADD381 pKa = 4.32 SSDD384 pKa = 3.47 LNYY387 pKa = 10.2 TVVYY391 pKa = 10.14 APAYY395 pKa = 9.39 SATSKK400 pKa = 9.89 TINEE404 pKa = 4.12 TXXXXNTSNTSNTNTSNTNTNTTDD428 pKa = 3.02 ANEE431 pKa = 4.06 YY432 pKa = 10.86 EE433 pKa = 4.3 RR434 pKa = 11.84 FKK436 pKa = 11.33 CGNTKK441 pKa = 10.5 GNEE444 pKa = 3.79
Molecular weight: 46.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.853
IPC2_protein 3.935
IPC_protein 3.973
Toseland 3.732
ProMoST 4.126
Dawson 3.973
Bjellqvist 4.151
Wikipedia 3.935
Rodwell 3.795
Grimsley 3.643
Solomon 3.973
Lehninger 3.935
Nozaki 4.088
DTASelect 4.38
Thurlkill 3.795
EMBOSS 3.935
Sillero 4.101
Patrickios 2.511
IPC_peptide 3.961
IPC2_peptide 4.062
IPC2.peptide.svr19 4.002
Protein with the highest isoelectric point:
>tr|A0A0R2CDN9|A0A0R2CDN9_9LACO ABC transporter ATP-binding protein OS=Lactobacillus cacaonum DSM 21116 OX=1423729 GN=FC80_GL001724 PE=4 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.69 QPKK8 pKa = 8.69 KK9 pKa = 7.77 RR10 pKa = 11.84 HH11 pKa = 4.99 RR12 pKa = 11.84 QRR14 pKa = 11.84 VHH16 pKa = 6.07 GFRR19 pKa = 11.84 KK20 pKa = 10.03 RR21 pKa = 11.84 MSTSNGRR28 pKa = 11.84 AVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 9.05 GRR39 pKa = 11.84 KK40 pKa = 8.7 VLSAA44 pKa = 4.05
Molecular weight: 5.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.449
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.135
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.082
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1801
0
1801
557821
44
1707
309.7
34.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.908 ± 0.053
0.554 ± 0.013
5.259 ± 0.059
6.392 ± 0.075
4.648 ± 0.057
6.493 ± 0.052
1.74 ± 0.022
8.34 ± 0.064
7.762 ± 0.054
9.878 ± 0.071
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.553 ± 0.027
5.43 ± 0.043
3.108 ± 0.034
3.814 ± 0.037
3.64 ± 0.038
6.481 ± 0.059
5.657 ± 0.048
6.903 ± 0.045
0.876 ± 0.019
3.561 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here