Hymenobacter gelipurpurascens

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Hymenobacteraceae; Hymenobacter

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4241 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A212T2X0|A0A212T2X0_9BACT Short-chain dehydrogenase OS=Hymenobacter gelipurpurascens OX=89968 GN=SAMN06265337_0241 PE=3 SV=1
MM1 pKa = 8.43DD2 pKa = 3.81YY3 pKa = 11.36TNDD6 pKa = 3.5PEE8 pKa = 5.64LNGKK12 pKa = 8.58YY13 pKa = 10.13LGTITKK19 pKa = 10.4DD20 pKa = 3.03FATVSDD26 pKa = 4.14TLSEE30 pKa = 4.07ASSQIRR36 pKa = 11.84KK37 pKa = 9.39RR38 pKa = 11.84DD39 pKa = 3.18ISKK42 pKa = 10.51YY43 pKa = 9.21PIFVFARR50 pKa = 11.84QEE52 pKa = 4.02VPLGGLLINADD63 pKa = 3.75EE64 pKa = 5.58ANLEE68 pKa = 4.0WHH70 pKa = 6.32VFASYY75 pKa = 11.36LEE77 pKa = 4.91LFVQQGIVSQEE88 pKa = 4.01GIEE91 pKa = 4.42AFQSTYY97 pKa = 10.62RR98 pKa = 11.84DD99 pKa = 3.27ADD101 pKa = 3.84EE102 pKa = 4.42YY103 pKa = 11.61CCLFVLDD110 pKa = 4.67EE111 pKa = 4.36EE112 pKa = 4.73FTNFVYY118 pKa = 10.29IPYY121 pKa = 10.15PEE123 pKa = 4.46DD124 pKa = 3.11

Molecular weight: 14.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A212UH86|A0A212UH86_9BACT Periplasmic chaperone for outer membrane proteins Skp OS=Hymenobacter gelipurpurascens OX=89968 GN=SAMN06265337_4018 PE=3 SV=1
MM1 pKa = 7.34AHH3 pKa = 7.5DD4 pKa = 3.84GTLGHH9 pKa = 6.94FLPFLLGRR17 pKa = 11.84LLRR20 pKa = 11.84AVLWPDD26 pKa = 3.08AGEE29 pKa = 4.19GRR31 pKa = 11.84AALIFLTLFCILYY44 pKa = 10.0GVVLAFMLNNEE55 pKa = 4.2NGIPSGFAQKK65 pKa = 11.02LLFALNGILYY75 pKa = 10.47ASALLVDD82 pKa = 5.36FVPTYY87 pKa = 10.84RR88 pKa = 11.84PVQPPLPDD96 pKa = 3.65HH97 pKa = 6.6FPVSGRR103 pKa = 11.84LNLITAFLLDD113 pKa = 5.42LITVRR118 pKa = 11.84RR119 pKa = 11.84FLLLLFLLVALACAPHH135 pKa = 6.13SWRR138 pKa = 11.84PLGLNLLVLLSAGAASFNLRR158 pKa = 11.84LLLSMGRR165 pKa = 11.84WRR167 pKa = 11.84HH168 pKa = 5.91LLFALNVLCLAAAAGWLSTFISLPLAATALVVVAIAGPLVLWGVALAGLGEE219 pKa = 4.43KK220 pKa = 10.48FNARR224 pKa = 11.84HH225 pKa = 5.79LAVAPEE231 pKa = 4.4SKK233 pKa = 10.57SEE235 pKa = 3.93NQLLARR241 pKa = 11.84LSPEE245 pKa = 2.99WKK247 pKa = 10.2AYY249 pKa = 9.69LRR251 pKa = 11.84KK252 pKa = 9.59CWPALSVALVAKK264 pKa = 10.03LVILGLSSQMTATRR278 pKa = 11.84HH279 pKa = 5.36GDD281 pKa = 3.41PLNGVFWMSFLPIISFTYY299 pKa = 10.35VNNNLFGIIGPVSANEE315 pKa = 3.87LARR318 pKa = 11.84LGLTKK323 pKa = 10.49RR324 pKa = 11.84LLYY327 pKa = 10.59LYY329 pKa = 9.9LRR331 pKa = 11.84LVGPVLLLDD340 pKa = 4.2CLLSVVVLLALFPQQRR356 pKa = 11.84WSLIGLIPLAAVAMLALGLWGSLYY380 pKa = 10.08KK381 pKa = 10.8AKK383 pKa = 10.5AISKK387 pKa = 10.55SIDD390 pKa = 3.42LANMRR395 pKa = 11.84NNASTLMNLLSIGVGAALFFMPWWWARR422 pKa = 11.84IVLAIVVMASAWWPVQRR439 pKa = 11.84VLRR442 pKa = 11.84NEE444 pKa = 3.95GGLRR448 pKa = 11.84RR449 pKa = 11.84QLWGALNSS457 pKa = 3.82

Molecular weight: 49.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4241

0

4241

1451777

29

3034

342.3

37.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.929 ± 0.046

0.708 ± 0.011

4.835 ± 0.026

5.493 ± 0.041

4.13 ± 0.024

7.507 ± 0.034

2.106 ± 0.019

4.607 ± 0.031

4.236 ± 0.037

10.992 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.0 ± 0.017

3.793 ± 0.035

5.106 ± 0.026

4.848 ± 0.032

5.873 ± 0.03

5.727 ± 0.032

6.236 ± 0.041

7.096 ± 0.028

1.246 ± 0.017

3.533 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski