Bovine faeces associated smacovirus 2

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cremevirales; Smacoviridae; Porprismacovirus; Porprismacovirus bovas1

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A160HX09|A0A160HX09_9VIRU Replication associated protein OS=Bovine faeces associated smacovirus 2 OX=1843750 PE=4 SV=1
MM1 pKa = 7.06PTITVRR7 pKa = 11.84EE8 pKa = 4.18TYY10 pKa = 10.58DD11 pKa = 4.1LSTAPNRR18 pKa = 11.84LGLIGIHH25 pKa = 5.86TPGSAIIRR33 pKa = 11.84KK34 pKa = 9.17LYY36 pKa = 9.37PGLWLNYY43 pKa = 9.98NKK45 pKa = 10.14VKK47 pKa = 10.9VNSCNVALACASLLPADD64 pKa = 4.31PLQIGVEE71 pKa = 4.09AGDD74 pKa = 4.41IAPQDD79 pKa = 3.84LFNPILYY86 pKa = 8.83RR87 pKa = 11.84ACSTDD92 pKa = 2.89SFNTILNRR100 pKa = 11.84MYY102 pKa = 10.4GTDD105 pKa = 4.06SMFNLQDD112 pKa = 3.26GSIAFAGGTNPGDD125 pKa = 4.04PFPSASDD132 pKa = 3.49TVSEE136 pKa = 3.98DD137 pKa = 3.07VYY139 pKa = 11.53YY140 pKa = 11.26GLLSEE145 pKa = 5.11SGWKK149 pKa = 9.88KK150 pKa = 11.0AMPQSGLVMRR160 pKa = 11.84NLVPLVYY167 pKa = 10.51NVISNYY173 pKa = 10.59GNGAKK178 pKa = 10.02LDD180 pKa = 4.24GPSEE184 pKa = 4.26PNDD187 pKa = 3.45YY188 pKa = 10.02KK189 pKa = 11.06QVVAVPSGSGGSQSEE204 pKa = 4.12FRR206 pKa = 11.84LASIFRR212 pKa = 11.84GHH214 pKa = 6.51SFPMPALPTARR225 pKa = 11.84GLVQDD230 pKa = 3.56VVGYY234 pKa = 10.64QDD236 pKa = 3.59TSSSAQSTVDD246 pKa = 3.54NSWSFPYY253 pKa = 10.27FPPTLIPKK261 pKa = 8.91TYY263 pKa = 8.67VGCVIIPPSKK273 pKa = 9.93LHH275 pKa = 6.17RR276 pKa = 11.84LYY278 pKa = 11.12FRR280 pKa = 11.84MTISWSVSFFEE291 pKa = 5.38PISIVEE297 pKa = 4.18RR298 pKa = 11.84GRR300 pKa = 11.84LTEE303 pKa = 3.98ISQVGIATYY312 pKa = 10.23GSSYY316 pKa = 11.43NFGEE320 pKa = 4.79SKK322 pKa = 10.83DD323 pKa = 4.68LEE325 pKa = 4.67DD326 pKa = 4.13KK327 pKa = 9.88LTNIEE332 pKa = 4.34STVDD336 pKa = 3.41TLDD339 pKa = 3.27VDD341 pKa = 4.56AKK343 pKa = 10.65MIMQSS348 pKa = 2.98

Molecular weight: 37.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A160HX09|A0A160HX09_9VIRU Replication associated protein OS=Bovine faeces associated smacovirus 2 OX=1843750 PE=4 SV=1
MM1 pKa = 7.33TGPRR5 pKa = 11.84TIHH8 pKa = 5.84KK9 pKa = 10.02RR10 pKa = 11.84IIRR13 pKa = 11.84EE14 pKa = 3.82IFKK17 pKa = 10.72KK18 pKa = 10.61CDD20 pKa = 3.08VKK22 pKa = 11.08KK23 pKa = 10.9YY24 pKa = 9.67IIAMEE29 pKa = 4.27TGNGGYY35 pKa = 7.41EE36 pKa = 3.68HH37 pKa = 6.35WQIRR41 pKa = 11.84CTASRR46 pKa = 11.84PDD48 pKa = 3.39FFEE51 pKa = 4.11YY52 pKa = 10.63VHH54 pKa = 7.13DD55 pKa = 4.15RR56 pKa = 11.84EE57 pKa = 4.5PRR59 pKa = 11.84FNIQKK64 pKa = 10.66AEE66 pKa = 4.14TEE68 pKa = 4.11SMEE71 pKa = 4.16YY72 pKa = 9.86EE73 pKa = 4.01RR74 pKa = 11.84KK75 pKa = 9.91EE76 pKa = 3.78GRR78 pKa = 11.84FWSSEE83 pKa = 3.62DD84 pKa = 3.25TTEE87 pKa = 4.32IRR89 pKa = 11.84QCRR92 pKa = 11.84FGEE95 pKa = 3.94LGGKK99 pKa = 8.34GRR101 pKa = 11.84EE102 pKa = 4.0WQRR105 pKa = 11.84QILKK109 pKa = 9.97VLKK112 pKa = 9.37NQDD115 pKa = 2.63VRR117 pKa = 11.84TIDD120 pKa = 3.5VVLDD124 pKa = 3.45PVGARR129 pKa = 11.84GKK131 pKa = 9.55SHH133 pKa = 6.11FTIALWEE140 pKa = 4.27RR141 pKa = 11.84GEE143 pKa = 3.99ALVVPRR149 pKa = 11.84YY150 pKa = 10.22SCTSEE155 pKa = 3.96KK156 pKa = 10.72LSAFVCSAYY165 pKa = 9.8KK166 pKa = 10.19GEE168 pKa = 4.57KK169 pKa = 9.55IIIIDD174 pKa = 3.53IPRR177 pKa = 11.84ANKK180 pKa = 7.67PTTALYY186 pKa = 10.95EE187 pKa = 4.44SMEE190 pKa = 4.02EE191 pKa = 4.1MKK193 pKa = 10.78DD194 pKa = 3.44GLVFDD199 pKa = 4.86PRR201 pKa = 11.84YY202 pKa = 9.88SGKK205 pKa = 8.15TRR207 pKa = 11.84NIRR210 pKa = 11.84GTKK213 pKa = 10.13VLVFTNNPLDD223 pKa = 4.06LKK225 pKa = 10.85KK226 pKa = 10.67LSHH229 pKa = 7.11DD230 pKa = 2.93RR231 pKa = 11.84WNLHH235 pKa = 6.02GITRR239 pKa = 11.84DD240 pKa = 3.51GTLSS244 pKa = 3.18

Molecular weight: 28.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

592

244

348

296.0

33.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.236 ± 0.655

1.52 ± 0.305

5.574 ± 0.142

5.574 ± 1.746

4.054 ± 0.026

7.77 ± 0.226

1.52 ± 0.541

6.926 ± 0.496

5.405 ± 1.608

7.601 ± 0.837

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.365 ± 0.182

4.392 ± 0.641

5.912 ± 1.281

2.872 ± 0.238

6.081 ± 1.927

8.784 ± 1.99

6.419 ± 0.316

6.419 ± 0.628

1.52 ± 0.305

4.054 ± 0.447
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski