Caulobacter phage Ccr34

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Dolichocephalovirinae; Shapirovirus; unclassified Shapirovirus

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 321 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V0EEQ0|A0A1V0EEQ0_9CAUD Integrase OS=Caulobacter phage Ccr34 OX=1959739 GN=Ccr34_gp148 PE=3 SV=1
MM1 pKa = 7.89RR2 pKa = 11.84EE3 pKa = 3.81GFMLEE8 pKa = 4.36GYY10 pKa = 9.94GGLKK14 pKa = 9.79IGPGGGADD22 pKa = 3.82PYY24 pKa = 8.94TTLGDD29 pKa = 4.04GGSEE33 pKa = 3.97GCIPVKK39 pKa = 10.77DD40 pKa = 5.34DD41 pKa = 3.22IAPDD45 pKa = 3.55GGTEE49 pKa = 3.9IISPHH54 pKa = 6.43GYY56 pKa = 9.87GG57 pKa = 4.0

Molecular weight: 5.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V0EF72|A0A1V0EF72_9CAUD Uncharacterized protein OS=Caulobacter phage Ccr34 OX=1959739 GN=Ccr34_gp318 PE=4 SV=1
MM1 pKa = 7.83RR2 pKa = 11.84PRR4 pKa = 11.84MFAFLRR10 pKa = 11.84RR11 pKa = 11.84LSLAAVHH18 pKa = 5.93GVLWAILLAMLALGGPRR35 pKa = 11.84RR36 pKa = 11.84APP38 pKa = 3.25

Molecular weight: 4.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

321

0

321

62090

34

1964

193.4

21.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.735 ± 0.198

0.897 ± 0.064

6.526 ± 0.159

6.263 ± 0.201

3.658 ± 0.097

8.158 ± 0.36

2.155 ± 0.094

4.622 ± 0.116

4.444 ± 0.171

8.278 ± 0.157

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.264 ± 0.085

2.912 ± 0.102

5.312 ± 0.147

3.181 ± 0.103

6.982 ± 0.203

4.961 ± 0.172

6.009 ± 0.233

6.896 ± 0.125

1.727 ± 0.075

3.021 ± 0.088
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski