Amino acid dipepetide frequency for Streptomyces phage Attoomi

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.694AlaAla: 16.694 ± 1.883
0.403AlaCys: 0.403 ± 0.199
8.79AlaAsp: 8.79 ± 0.826
10.0AlaGlu: 10.0 ± 1.47
2.339AlaPhe: 2.339 ± 0.501
11.855AlaGly: 11.855 ± 1.276
2.177AlaHis: 2.177 ± 0.399
4.194AlaIle: 4.194 ± 0.644
6.774AlaLys: 6.774 ± 1.17
12.581AlaLeu: 12.581 ± 1.116
3.306AlaMet: 3.306 ± 0.676
2.823AlaAsn: 2.823 ± 0.489
7.258AlaPro: 7.258 ± 0.948
4.032AlaGln: 4.032 ± 0.767
11.532AlaArg: 11.532 ± 0.911
5.484AlaSer: 5.484 ± 0.869
7.823AlaThr: 7.823 ± 1.74
11.855AlaVal: 11.855 ± 0.813
2.258AlaTrp: 2.258 ± 0.358
2.258AlaTyr: 2.258 ± 0.489
0.0AlaXaa: 0.0 ± 0.0
Cys
0.968CysAla: 0.968 ± 0.249
0.0CysCys: 0.0 ± 0.0
0.806CysAsp: 0.806 ± 0.257
0.161CysGlu: 0.161 ± 0.102
0.0CysPhe: 0.0 ± 0.0
0.565CysGly: 0.565 ± 0.246
0.323CysHis: 0.323 ± 0.144
0.081CysIle: 0.081 ± 0.085
0.081CysLys: 0.081 ± 0.091
0.161CysLeu: 0.161 ± 0.121
0.0CysMet: 0.0 ± 0.0
0.081CysAsn: 0.081 ± 0.084
0.645CysPro: 0.645 ± 0.235
0.242CysGln: 0.242 ± 0.172
0.726CysArg: 0.726 ± 0.247
0.242CysSer: 0.242 ± 0.131
0.323CysThr: 0.323 ± 0.185
0.403CysVal: 0.403 ± 0.172
0.242CysTrp: 0.242 ± 0.125
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
9.758AspAla: 9.758 ± 1.159
0.323AspCys: 0.323 ± 0.159
2.984AspAsp: 2.984 ± 0.578
1.532AspGlu: 1.532 ± 0.284
1.613AspPhe: 1.613 ± 0.381
6.21AspGly: 6.21 ± 0.523
1.532AspHis: 1.532 ± 0.381
1.452AspIle: 1.452 ± 0.415
1.532AspLys: 1.532 ± 0.307
5.726AspLeu: 5.726 ± 0.934
1.452AspMet: 1.452 ± 0.278
1.29AspAsn: 1.29 ± 0.288
5.161AspPro: 5.161 ± 0.644
1.694AspGln: 1.694 ± 0.408
6.371AspArg: 6.371 ± 0.743
2.258AspSer: 2.258 ± 0.368
4.597AspThr: 4.597 ± 0.774
5.726AspVal: 5.726 ± 0.719
1.532AspTrp: 1.532 ± 0.377
0.968AspTyr: 0.968 ± 0.247
0.0AspXaa: 0.0 ± 0.0
Glu
7.984GluAla: 7.984 ± 0.854
0.403GluCys: 0.403 ± 0.211
4.113GluAsp: 4.113 ± 0.661
3.387GluGlu: 3.387 ± 0.635
2.016GluPhe: 2.016 ± 0.553
5.0GluGly: 5.0 ± 0.797
1.21GluHis: 1.21 ± 0.269
1.935GluIle: 1.935 ± 0.375
0.887GluLys: 0.887 ± 0.242
6.532GluLeu: 6.532 ± 0.911
1.129GluMet: 1.129 ± 0.27
1.048GluAsn: 1.048 ± 0.259
4.597GluPro: 4.597 ± 0.858
1.129GluGln: 1.129 ± 0.3
5.565GluArg: 5.565 ± 0.857
4.032GluSer: 4.032 ± 0.61
4.194GluThr: 4.194 ± 0.527
3.468GluVal: 3.468 ± 0.584
1.532GluTrp: 1.532 ± 0.266
1.774GluTyr: 1.774 ± 0.448
0.0GluXaa: 0.0 ± 0.0
Phe
2.016PheAla: 2.016 ± 0.368
0.081PheCys: 0.081 ± 0.084
1.452PheAsp: 1.452 ± 0.445
0.968PheGlu: 0.968 ± 0.243
0.403PhePhe: 0.403 ± 0.202
2.339PheGly: 2.339 ± 0.403
0.081PheHis: 0.081 ± 0.069
0.726PheIle: 0.726 ± 0.239
1.129PheLys: 1.129 ± 0.304
3.065PheLeu: 3.065 ± 0.666
0.645PheMet: 0.645 ± 0.26
0.403PheAsn: 0.403 ± 0.195
1.29PhePro: 1.29 ± 0.564
1.21PheGln: 1.21 ± 0.267
2.823PheArg: 2.823 ± 0.717
0.806PheSer: 0.806 ± 0.211
2.097PheThr: 2.097 ± 0.377
1.532PheVal: 1.532 ± 0.278
0.161PheTrp: 0.161 ± 0.112
0.565PheTyr: 0.565 ± 0.237
0.0PheXaa: 0.0 ± 0.0
Gly
8.871GlyAla: 8.871 ± 1.217
0.806GlyCys: 0.806 ± 0.277
4.516GlyAsp: 4.516 ± 0.485
4.919GlyGlu: 4.919 ± 0.542
2.177GlyPhe: 2.177 ± 0.416
7.823GlyGly: 7.823 ± 0.847
1.29GlyHis: 1.29 ± 0.294
2.5GlyIle: 2.5 ± 0.594
4.194GlyLys: 4.194 ± 0.744
6.855GlyLeu: 6.855 ± 0.954
2.016GlyMet: 2.016 ± 0.427
2.097GlyAsn: 2.097 ± 0.416
5.323GlyPro: 5.323 ± 0.614
3.79GlyGln: 3.79 ± 0.678
7.581GlyArg: 7.581 ± 0.741
4.194GlySer: 4.194 ± 0.942
5.323GlyThr: 5.323 ± 0.82
8.468GlyVal: 8.468 ± 0.921
2.016GlyTrp: 2.016 ± 0.476
2.339GlyTyr: 2.339 ± 0.432
0.0GlyXaa: 0.0 ± 0.0
His
2.5HisAla: 2.5 ± 0.42
0.161HisCys: 0.161 ± 0.105
1.532HisAsp: 1.532 ± 0.391
0.484HisGlu: 0.484 ± 0.209
0.484HisPhe: 0.484 ± 0.206
1.29HisGly: 1.29 ± 0.306
0.403HisHis: 0.403 ± 0.159
0.645HisIle: 0.645 ± 0.184
0.161HisLys: 0.161 ± 0.11
0.806HisLeu: 0.806 ± 0.287
0.403HisMet: 0.403 ± 0.167
0.323HisAsn: 0.323 ± 0.14
1.855HisPro: 1.855 ± 0.388
0.242HisGln: 0.242 ± 0.129
1.21HisArg: 1.21 ± 0.303
0.645HisSer: 0.645 ± 0.271
0.645HisThr: 0.645 ± 0.222
1.855HisVal: 1.855 ± 0.564
0.484HisTrp: 0.484 ± 0.162
0.403HisTyr: 0.403 ± 0.175
0.0HisXaa: 0.0 ± 0.0
Ile
2.903IleAla: 2.903 ± 0.623
0.242IleCys: 0.242 ± 0.161
1.774IleAsp: 1.774 ± 0.413
1.371IleGlu: 1.371 ± 0.414
0.645IlePhe: 0.645 ± 0.276
2.581IleGly: 2.581 ± 0.611
0.887IleHis: 0.887 ± 0.3
0.403IleIle: 0.403 ± 0.182
1.371IleLys: 1.371 ± 0.508
1.935IleLeu: 1.935 ± 0.537
0.565IleMet: 0.565 ± 0.22
0.806IleAsn: 0.806 ± 0.263
3.065IlePro: 3.065 ± 0.523
0.968IleGln: 0.968 ± 0.303
3.306IleArg: 3.306 ± 0.471
1.048IleSer: 1.048 ± 0.264
3.065IleThr: 3.065 ± 0.543
1.935IleVal: 1.935 ± 0.323
0.403IleTrp: 0.403 ± 0.162
0.565IleTyr: 0.565 ± 0.214
0.0IleXaa: 0.0 ± 0.0
Lys
6.694LysAla: 6.694 ± 1.2
0.242LysCys: 0.242 ± 0.129
2.823LysAsp: 2.823 ± 0.476
1.29LysGlu: 1.29 ± 0.371
1.29LysPhe: 1.29 ± 0.32
4.113LysGly: 4.113 ± 0.67
0.484LysHis: 0.484 ± 0.195
1.29LysIle: 1.29 ± 0.448
0.645LysLys: 0.645 ± 0.275
2.823LysLeu: 2.823 ± 0.551
0.242LysMet: 0.242 ± 0.12
0.726LysAsn: 0.726 ± 0.276
2.016LysPro: 2.016 ± 0.562
0.242LysGln: 0.242 ± 0.135
3.145LysArg: 3.145 ± 0.584
2.258LysSer: 2.258 ± 0.816
1.855LysThr: 1.855 ± 0.356
2.661LysVal: 2.661 ± 0.515
0.565LysTrp: 0.565 ± 0.195
0.968LysTyr: 0.968 ± 0.255
0.0LysXaa: 0.0 ± 0.0
Leu
14.113LeuAla: 14.113 ± 1.31
0.484LeuCys: 0.484 ± 0.203
5.806LeuAsp: 5.806 ± 0.929
8.065LeuGlu: 8.065 ± 1.164
2.097LeuPhe: 2.097 ± 0.371
6.855LeuGly: 6.855 ± 1.21
1.048LeuHis: 1.048 ± 0.292
1.774LeuIle: 1.774 ± 0.491
4.032LeuLys: 4.032 ± 0.487
5.887LeuLeu: 5.887 ± 0.943
2.5LeuMet: 2.5 ± 0.389
2.016LeuAsn: 2.016 ± 0.492
4.677LeuPro: 4.677 ± 0.726
2.661LeuGln: 2.661 ± 0.411
5.645LeuArg: 5.645 ± 0.828
4.435LeuSer: 4.435 ± 0.687
5.0LeuThr: 5.0 ± 0.586
7.339LeuVal: 7.339 ± 0.745
0.726LeuTrp: 0.726 ± 0.264
1.855LeuTyr: 1.855 ± 0.371
0.0LeuXaa: 0.0 ± 0.0
Met
4.355MetAla: 4.355 ± 0.796
0.242MetCys: 0.242 ± 0.129
1.048MetAsp: 1.048 ± 0.222
0.887MetGlu: 0.887 ± 0.248
0.565MetPhe: 0.565 ± 0.192
0.968MetGly: 0.968 ± 0.254
0.242MetHis: 0.242 ± 0.123
0.806MetIle: 0.806 ± 0.287
1.048MetLys: 1.048 ± 0.309
1.532MetLeu: 1.532 ± 0.303
0.081MetMet: 0.081 ± 0.085
0.403MetAsn: 0.403 ± 0.15
1.532MetPro: 1.532 ± 0.233
0.323MetGln: 0.323 ± 0.165
1.935MetArg: 1.935 ± 0.445
1.694MetSer: 1.694 ± 0.519
2.016MetThr: 2.016 ± 0.38
0.968MetVal: 0.968 ± 0.3
0.081MetTrp: 0.081 ± 0.081
0.323MetTyr: 0.323 ± 0.146
0.0MetXaa: 0.0 ± 0.0
Asn
3.065AsnAla: 3.065 ± 0.473
0.0AsnCys: 0.0 ± 0.0
1.129AsnAsp: 1.129 ± 0.31
0.484AsnGlu: 0.484 ± 0.23
0.323AsnPhe: 0.323 ± 0.185
2.903AsnGly: 2.903 ± 0.464
0.242AsnHis: 0.242 ± 0.117
0.645AsnIle: 0.645 ± 0.201
0.484AsnLys: 0.484 ± 0.229
2.177AsnLeu: 2.177 ± 0.415
0.403AsnMet: 0.403 ± 0.174
0.484AsnAsn: 0.484 ± 0.204
1.935AsnPro: 1.935 ± 0.425
0.484AsnGln: 0.484 ± 0.164
1.613AsnArg: 1.613 ± 0.417
0.484AsnSer: 0.484 ± 0.146
1.21AsnThr: 1.21 ± 0.262
1.855AsnVal: 1.855 ± 0.366
0.323AsnTrp: 0.323 ± 0.16
0.323AsnTyr: 0.323 ± 0.139
0.0AsnXaa: 0.0 ± 0.0
Pro
9.597ProAla: 9.597 ± 1.018
0.645ProCys: 0.645 ± 0.209
5.645ProAsp: 5.645 ± 0.724
6.774ProGlu: 6.774 ± 1.012
1.21ProPhe: 1.21 ± 0.271
5.806ProGly: 5.806 ± 0.961
0.968ProHis: 0.968 ± 0.292
1.935ProIle: 1.935 ± 0.584
2.177ProLys: 2.177 ± 0.477
4.032ProLeu: 4.032 ± 0.641
1.29ProMet: 1.29 ± 0.304
0.887ProAsn: 0.887 ± 0.258
2.5ProPro: 2.5 ± 0.485
1.048ProGln: 1.048 ± 0.257
3.548ProArg: 3.548 ± 0.652
2.661ProSer: 2.661 ± 0.448
2.823ProThr: 2.823 ± 0.613
6.532ProVal: 6.532 ± 0.837
1.129ProTrp: 1.129 ± 0.305
1.29ProTyr: 1.29 ± 0.361
0.0ProXaa: 0.0 ± 0.0
Gln
4.113GlnAla: 4.113 ± 0.459
0.081GlnCys: 0.081 ± 0.082
1.371GlnAsp: 1.371 ± 0.323
2.903GlnGlu: 2.903 ± 0.624
1.21GlnPhe: 1.21 ± 0.28
2.903GlnGly: 2.903 ± 0.549
0.242GlnHis: 0.242 ± 0.143
0.887GlnIle: 0.887 ± 0.394
0.161GlnLys: 0.161 ± 0.152
3.306GlnLeu: 3.306 ± 0.789
0.887GlnMet: 0.887 ± 0.256
0.403GlnAsn: 0.403 ± 0.191
1.21GlnPro: 1.21 ± 0.303
0.403GlnGln: 0.403 ± 0.163
1.613GlnArg: 1.613 ± 0.255
2.016GlnSer: 2.016 ± 0.377
1.855GlnThr: 1.855 ± 0.465
3.226GlnVal: 3.226 ± 0.418
0.403GlnTrp: 0.403 ± 0.209
0.887GlnTyr: 0.887 ± 0.277
0.0GlnXaa: 0.0 ± 0.0
Arg
9.839ArgAla: 9.839 ± 0.733
0.565ArgCys: 0.565 ± 0.217
5.242ArgAsp: 5.242 ± 0.619
5.565ArgGlu: 5.565 ± 0.885
2.016ArgPhe: 2.016 ± 0.508
5.242ArgGly: 5.242 ± 0.845
1.855ArgHis: 1.855 ± 0.503
2.661ArgIle: 2.661 ± 0.433
2.903ArgLys: 2.903 ± 0.557
7.419ArgLeu: 7.419 ± 0.809
2.097ArgMet: 2.097 ± 0.442
2.097ArgAsn: 2.097 ± 0.382
4.758ArgPro: 4.758 ± 0.763
2.5ArgGln: 2.5 ± 0.375
5.645ArgArg: 5.645 ± 0.975
3.387ArgSer: 3.387 ± 0.514
4.597ArgThr: 4.597 ± 0.563
7.661ArgVal: 7.661 ± 1.008
1.694ArgTrp: 1.694 ± 0.415
1.774ArgTyr: 1.774 ± 0.349
0.0ArgXaa: 0.0 ± 0.0
Ser
6.694SerAla: 6.694 ± 0.89
0.161SerCys: 0.161 ± 0.123
2.742SerAsp: 2.742 ± 0.416
1.532SerGlu: 1.532 ± 0.33
1.129SerPhe: 1.129 ± 0.242
5.403SerGly: 5.403 ± 0.708
0.565SerHis: 0.565 ± 0.184
2.097SerIle: 2.097 ± 0.474
2.258SerLys: 2.258 ± 0.614
4.274SerLeu: 4.274 ± 0.807
0.968SerMet: 0.968 ± 0.276
1.29SerAsn: 1.29 ± 0.35
2.419SerPro: 2.419 ± 0.363
1.774SerGln: 1.774 ± 0.298
2.984SerArg: 2.984 ± 0.456
1.613SerSer: 1.613 ± 0.336
2.258SerThr: 2.258 ± 0.405
4.839SerVal: 4.839 ± 0.835
0.726SerTrp: 0.726 ± 0.236
1.21SerTyr: 1.21 ± 0.312
0.0SerXaa: 0.0 ± 0.0
Thr
8.871ThrAla: 8.871 ± 1.183
0.161ThrCys: 0.161 ± 0.111
3.79ThrAsp: 3.79 ± 0.588
4.032ThrGlu: 4.032 ± 0.68
2.339ThrPhe: 2.339 ± 0.47
5.887ThrGly: 5.887 ± 0.829
0.887ThrHis: 0.887 ± 0.262
2.097ThrIle: 2.097 ± 0.396
2.097ThrLys: 2.097 ± 0.546
6.452ThrLeu: 6.452 ± 0.703
0.968ThrMet: 0.968 ± 0.277
1.048ThrAsn: 1.048 ± 0.281
3.71ThrPro: 3.71 ± 0.615
2.419ThrGln: 2.419 ± 0.457
3.145ThrArg: 3.145 ± 0.398
2.742ThrSer: 2.742 ± 0.548
4.355ThrThr: 4.355 ± 0.649
6.935ThrVal: 6.935 ± 0.984
0.887ThrTrp: 0.887 ± 0.245
1.532ThrTyr: 1.532 ± 0.433
0.0ThrXaa: 0.0 ± 0.0
Val
10.403ValAla: 10.403 ± 0.909
0.887ValCys: 0.887 ± 0.255
5.645ValAsp: 5.645 ± 0.725
5.565ValGlu: 5.565 ± 0.712
1.371ValPhe: 1.371 ± 0.374
5.806ValGly: 5.806 ± 0.614
1.129ValHis: 1.129 ± 0.29
2.581ValIle: 2.581 ± 0.38
3.629ValLys: 3.629 ± 0.583
7.742ValLeu: 7.742 ± 0.773
1.613ValMet: 1.613 ± 0.383
1.452ValAsn: 1.452 ± 0.263
6.371ValPro: 6.371 ± 0.772
3.065ValGln: 3.065 ± 0.451
7.581ValArg: 7.581 ± 0.899
4.435ValSer: 4.435 ± 0.47
7.581ValThr: 7.581 ± 1.277
8.468ValVal: 8.468 ± 0.961
1.694ValTrp: 1.694 ± 0.465
2.016ValTyr: 2.016 ± 0.403
0.0ValXaa: 0.0 ± 0.0
Trp
1.855TrpAla: 1.855 ± 0.469
0.0TrpCys: 0.0 ± 0.0
0.726TrpAsp: 0.726 ± 0.271
1.532TrpGlu: 1.532 ± 0.383
0.242TrpPhe: 0.242 ± 0.133
0.726TrpGly: 0.726 ± 0.24
0.565TrpHis: 0.565 ± 0.193
0.403TrpIle: 0.403 ± 0.177
0.484TrpLys: 0.484 ± 0.226
1.613TrpLeu: 1.613 ± 0.472
0.161TrpMet: 0.161 ± 0.107
0.484TrpAsn: 0.484 ± 0.229
1.371TrpPro: 1.371 ± 0.344
0.726TrpGln: 0.726 ± 0.232
1.935TrpArg: 1.935 ± 0.383
1.371TrpSer: 1.371 ± 0.299
1.371TrpThr: 1.371 ± 0.318
1.129TrpVal: 1.129 ± 0.256
0.242TrpTrp: 0.242 ± 0.173
0.484TrpTyr: 0.484 ± 0.174
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.226TyrAla: 3.226 ± 0.529
0.161TyrCys: 0.161 ± 0.118
1.774TyrAsp: 1.774 ± 0.332
0.484TyrGlu: 0.484 ± 0.155
0.323TyrPhe: 0.323 ± 0.144
2.823TyrGly: 2.823 ± 0.471
0.403TyrHis: 0.403 ± 0.187
0.726TyrIle: 0.726 ± 0.232
0.323TyrLys: 0.323 ± 0.181
2.097TyrLeu: 2.097 ± 0.344
0.242TyrMet: 0.242 ± 0.119
0.403TyrAsn: 0.403 ± 0.196
0.806TyrPro: 0.806 ± 0.267
1.129TyrGln: 1.129 ± 0.432
1.613TyrArg: 1.613 ± 0.361
1.129TyrSer: 1.129 ± 0.297
1.371TyrThr: 1.371 ± 0.299
2.177TyrVal: 2.177 ± 0.58
0.323TyrTrp: 0.323 ± 0.183
0.242TyrTyr: 0.242 ± 0.129
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 53 proteins (12401 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski