Amino acid dipepetide frequency for Methylococcaceae bacterium FWC3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	14.789AlaAla: 14.789 ± 0.162	1.328AlaCys: 1.328 ± 0.036	6.043AlaAsp: 6.043 ± 0.104	7.461AlaGlu: 7.461 ± 0.104	4.006AlaPhe: 4.006 ± 0.07	9.451AlaGly: 9.451 ± 0.123	2.219AlaHis: 2.219 ± 0.046	5.394AlaIle: 5.394 ± 0.083	3.052AlaLys: 3.052 ± 0.064	13.277AlaLeu: 13.277 ± 0.159	2.778AlaMet: 2.778 ± 0.05	2.691AlaAsn: 2.691 ± 0.059	5.078AlaPro: 5.078 ± 0.083	3.794AlaGln: 3.794 ± 0.065	8.038AlaArg: 8.038 ± 0.109	5.809AlaSer: 5.809 ± 0.085	5.654AlaThr: 5.654 ± 0.076	8.548AlaVal: 8.548 ± 0.105	1.684AlaTrp: 1.684 ± 0.046	2.679AlaTyr: 2.679 ± 0.058	0.0AlaXaa: 0.0 ± 0.0
Cys	1.187CysAla: 1.187 ± 0.037	0.166CysCys: 0.166 ± 0.013	0.582CysAsp: 0.582 ± 0.026	0.621CysGlu: 0.621 ± 0.027	0.364CysPhe: 0.364 ± 0.018	1.112CysGly: 1.112 ± 0.033	0.324CysHis: 0.324 ± 0.019	0.435CysIle: 0.435 ± 0.025	0.227CysLys: 0.227 ± 0.017	1.095CysLeu: 1.095 ± 0.033	0.158CysMet: 0.158 ± 0.013	0.289CysAsn: 0.289 ± 0.017	0.626CysPro: 0.626 ± 0.027	0.335CysGln: 0.335 ± 0.018	0.974CysArg: 0.974 ± 0.039	0.666CysSer: 0.666 ± 0.032	0.499CysThr: 0.499 ± 0.024	0.736CysVal: 0.736 ± 0.029	0.139CysTrp: 0.139 ± 0.011	0.296CysTyr: 0.296 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	6.222AspAla: 6.222 ± 0.094	0.644AspCys: 0.644 ± 0.025	3.094AspAsp: 3.094 ± 0.062	3.553AspGlu: 3.553 ± 0.069	2.333AspPhe: 2.333 ± 0.052	4.796AspGly: 4.796 ± 0.092	1.24AspHis: 1.24 ± 0.038	2.737AspIle: 2.737 ± 0.057	1.358AspLys: 1.358 ± 0.036	5.981AspLeu: 5.981 ± 0.079	0.975AspMet: 0.975 ± 0.028	1.174AspAsn: 1.174 ± 0.039	3.437AspPro: 3.437 ± 0.061	1.819AspGln: 1.819 ± 0.04	4.581AspArg: 4.581 ± 0.067	2.649AspSer: 2.649 ± 0.058	2.741AspThr: 2.741 ± 0.061	3.722AspVal: 3.722 ± 0.067	1.042AspTrp: 1.042 ± 0.032	1.739AspTyr: 1.739 ± 0.039	0.0AspXaa: 0.0 ± 0.0
Glu	6.88GluAla: 6.88 ± 0.109	0.518GluCys: 0.518 ± 0.025	2.65GluAsp: 2.65 ± 0.056	3.042GluGlu: 3.042 ± 0.068	2.149GluPhe: 2.149 ± 0.05	3.861GluGly: 3.861 ± 0.063	1.564GluHis: 1.564 ± 0.041	3.239GluIle: 3.239 ± 0.056	1.948GluLys: 1.948 ± 0.047	6.671GluLeu: 6.671 ± 0.082	1.163GluMet: 1.163 ± 0.038	1.448GluAsn: 1.448 ± 0.039	2.959GluPro: 2.959 ± 0.065	2.685GluGln: 2.685 ± 0.062	5.627GluArg: 5.627 ± 0.083	3.158GluSer: 3.158 ± 0.061	3.12GluThr: 3.12 ± 0.055	4.021GluVal: 4.021 ± 0.073	0.866GluTrp: 0.866 ± 0.03	1.282GluTyr: 1.282 ± 0.037	0.0GluXaa: 0.0 ± 0.0
Phe	4.032PheAla: 4.032 ± 0.07	0.461PheCys: 0.461 ± 0.021	2.534PheAsp: 2.534 ± 0.058	2.347PheGlu: 2.347 ± 0.052	1.596PhePhe: 1.596 ± 0.045	3.585PheGly: 3.585 ± 0.058	0.834PheHis: 0.834 ± 0.03	1.619PheIle: 1.619 ± 0.044	0.96PheLys: 0.96 ± 0.036	3.749PheLeu: 3.749 ± 0.072	0.732PheMet: 0.732 ± 0.03	1.067PheAsn: 1.067 ± 0.036	1.868PhePro: 1.868 ± 0.047	1.134PheGln: 1.134 ± 0.035	2.727PheArg: 2.727 ± 0.054	2.322PheSer: 2.322 ± 0.049	1.914PheThr: 1.914 ± 0.045	2.998PheVal: 2.998 ± 0.059	0.628PheTrp: 0.628 ± 0.026	0.977PheTyr: 0.977 ± 0.033	0.0PheXaa: 0.0 ± 0.0
Gly	8.057GlyAla: 8.057 ± 0.1	1.129GlyCys: 1.129 ± 0.034	4.383GlyAsp: 4.383 ± 0.071	4.427GlyGlu: 4.427 ± 0.078	3.479GlyPhe: 3.479 ± 0.063	6.536GlyGly: 6.536 ± 0.106	1.897GlyHis: 1.897 ± 0.048	4.313GlyIle: 4.313 ± 0.078	2.858GlyLys: 2.858 ± 0.065	9.037GlyLeu: 9.037 ± 0.096	1.967GlyMet: 1.967 ± 0.055	2.262GlyAsn: 2.262 ± 0.054	3.32GlyPro: 3.32 ± 0.114	3.0GlyGln: 3.0 ± 0.059	6.088GlyArg: 6.088 ± 0.085	4.748GlySer: 4.748 ± 0.087	4.49GlyThr: 4.49 ± 0.099	5.878GlyVal: 5.878 ± 0.082	1.333GlyTrp: 1.333 ± 0.039	2.382GlyTyr: 2.382 ± 0.054	0.0GlyXaa: 0.0 ± 0.0
His	2.378HisAla: 2.378 ± 0.052	0.32HisCys: 0.32 ± 0.02	1.227HisAsp: 1.227 ± 0.038	1.201HisGlu: 1.201 ± 0.037	0.964HisPhe: 0.964 ± 0.031	1.968HisGly: 1.968 ± 0.049	0.692HisHis: 0.692 ± 0.032	0.912HisIle: 0.912 ± 0.032	0.502HisLys: 0.502 ± 0.023	2.501HisLeu: 2.501 ± 0.055	0.428HisMet: 0.428 ± 0.022	0.485HisAsn: 0.485 ± 0.022	1.603HisPro: 1.603 ± 0.049	0.742HisGln: 0.742 ± 0.028	1.97HisArg: 1.97 ± 0.051	1.16HisSer: 1.16 ± 0.035	0.987HisThr: 0.987 ± 0.033	1.539HisVal: 1.539 ± 0.04	0.428HisTrp: 0.428 ± 0.025	0.713HisTyr: 0.713 ± 0.032	0.0HisXaa: 0.0 ± 0.0
Ile	6.144IleAla: 6.144 ± 0.088	0.478IleCys: 0.478 ± 0.022	3.256IleAsp: 3.256 ± 0.064	3.401IleGlu: 3.401 ± 0.069	1.405IlePhe: 1.405 ± 0.037	4.448IleGly: 4.448 ± 0.074	1.033IleHis: 1.033 ± 0.033	1.854IleIle: 1.854 ± 0.048	1.404IleLys: 1.404 ± 0.043	4.217IleLeu: 4.217 ± 0.062	0.753IleMet: 0.753 ± 0.031	1.384IleAsn: 1.384 ± 0.038	2.612IlePro: 2.612 ± 0.052	1.332IleGln: 1.332 ± 0.032	3.547IleArg: 3.547 ± 0.056	2.496IleSer: 2.496 ± 0.053	2.53IleThr: 2.53 ± 0.058	3.787IleVal: 3.787 ± 0.074	0.573IleTrp: 0.573 ± 0.024	1.18IleTyr: 1.18 ± 0.038	0.0IleXaa: 0.0 ± 0.0
Lys	3.244LysAla: 3.244 ± 0.065	0.188LysCys: 0.188 ± 0.015	1.522LysAsp: 1.522 ± 0.048	1.517LysGlu: 1.517 ± 0.047	0.788LysPhe: 0.788 ± 0.027	2.174LysGly: 2.174 ± 0.07	0.614LysHis: 0.614 ± 0.028	1.404LysIle: 1.404 ± 0.042	1.063LysLys: 1.063 ± 0.045	3.049LysLeu: 3.049 ± 0.058	0.56LysMet: 0.56 ± 0.028	0.796LysAsn: 0.796 ± 0.03	1.909LysPro: 1.909 ± 0.051	1.106LysGln: 1.106 ± 0.036	2.15LysArg: 2.15 ± 0.054	1.669LysSer: 1.669 ± 0.044	1.768LysThr: 1.768 ± 0.045	2.025LysVal: 2.025 ± 0.053	0.296LysTrp: 0.296 ± 0.019	0.608LysTyr: 0.608 ± 0.028	0.0LysXaa: 0.0 ± 0.0
Leu	13.374LeuAla: 13.374 ± 0.143	1.168LeuCys: 1.168 ± 0.041	6.267LeuAsp: 6.267 ± 0.088	6.215LeuGlu: 6.215 ± 0.079	4.122LeuPhe: 4.122 ± 0.087	8.656LeuGly: 8.656 ± 0.118	2.305LeuHis: 2.305 ± 0.049	5.334LeuIle: 5.334 ± 0.078	3.284LeuLys: 3.284 ± 0.06	11.951LeuLeu: 11.951 ± 0.169	2.287LeuMet: 2.287 ± 0.05	2.804LeuAsn: 2.804 ± 0.054	6.256LeuPro: 6.256 ± 0.089	3.773LeuGln: 3.773 ± 0.068	8.414LeuArg: 8.414 ± 0.101	6.799LeuSer: 6.799 ± 0.093	5.858LeuThr: 5.858 ± 0.079	7.487LeuVal: 7.487 ± 0.095	1.461LeuTrp: 1.461 ± 0.04	2.399LeuTyr: 2.399 ± 0.052	0.0LeuXaa: 0.0 ± 0.0
Met	2.336MetAla: 2.336 ± 0.043	0.15MetCys: 0.15 ± 0.013	1.062MetAsp: 1.062 ± 0.036	1.015MetGlu: 1.015 ± 0.033	0.6MetPhe: 0.6 ± 0.029	1.513MetGly: 1.513 ± 0.046	0.427MetHis: 0.427 ± 0.022	1.042MetIle: 1.042 ± 0.032	0.858MetLys: 0.858 ± 0.035	2.204MetLeu: 2.204 ± 0.051	0.447MetMet: 0.447 ± 0.024	0.777MetAsn: 0.777 ± 0.026	1.219MetPro: 1.219 ± 0.032	0.758MetGln: 0.758 ± 0.026	1.485MetArg: 1.485 ± 0.045	1.332MetSer: 1.332 ± 0.038	1.439MetThr: 1.439 ± 0.041	1.429MetVal: 1.429 ± 0.039	0.175MetTrp: 0.175 ± 0.014	0.312MetTyr: 0.312 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	2.978AsnAla: 2.978 ± 0.063	0.295AsnCys: 0.295 ± 0.018	1.396AsnAsp: 1.396 ± 0.045	1.313AsnGlu: 1.313 ± 0.038	0.994AsnPhe: 0.994 ± 0.033	2.262AsnGly: 2.262 ± 0.068	0.538AsnHis: 0.538 ± 0.027	1.182AsnIle: 1.182 ± 0.039	0.642AsnLys: 0.642 ± 0.026	2.8AsnLeu: 2.8 ± 0.061	0.463AsnMet: 0.463 ± 0.019	0.638AsnAsn: 0.638 ± 0.033	1.996AsnPro: 1.996 ± 0.048	0.855AsnGln: 0.855 ± 0.034	2.08AsnArg: 2.08 ± 0.053	1.262AsnSer: 1.262 ± 0.041	1.253AsnThr: 1.253 ± 0.041	1.926AsnVal: 1.926 ± 0.045	0.419AsnTrp: 0.419 ± 0.021	0.716AsnTyr: 0.716 ± 0.028	0.0AsnXaa: 0.0 ± 0.0
Pro	6.054ProAla: 6.054 ± 0.089	0.479ProCys: 0.479 ± 0.025	3.771ProAsp: 3.771 ± 0.07	4.127ProGlu: 4.127 ± 0.061	2.043ProPhe: 2.043 ± 0.044	4.768ProGly: 4.768 ± 0.082	1.122ProHis: 1.122 ± 0.038	2.264ProIle: 2.264 ± 0.05	1.465ProLys: 1.465 ± 0.049	5.267ProLeu: 5.267 ± 0.08	1.143ProMet: 1.143 ± 0.035	1.363ProAsn: 1.363 ± 0.04	2.97ProPro: 2.97 ± 0.071	1.594ProGln: 1.594 ± 0.044	3.24ProArg: 3.24 ± 0.06	2.832ProSer: 2.832 ± 0.054	2.655ProThr: 2.655 ± 0.055	4.219ProVal: 4.219 ± 0.066	0.803ProTrp: 0.803 ± 0.035	1.297ProTyr: 1.297 ± 0.042	0.0ProXaa: 0.0 ± 0.0
Gln	4.207GlnAla: 4.207 ± 0.076	0.338GlnCys: 0.338 ± 0.019	1.547GlnAsp: 1.547 ± 0.038	1.673GlnGlu: 1.673 ± 0.042	1.224GlnPhe: 1.224 ± 0.04	2.633GlnGly: 2.633 ± 0.058	0.874GlnHis: 0.874 ± 0.03	1.764GlnIle: 1.764 ± 0.05	0.95GlnLys: 0.95 ± 0.034	3.762GlnLeu: 3.762 ± 0.07	0.753GlnMet: 0.753 ± 0.026	0.857GlnAsn: 0.857 ± 0.031	1.943GlnPro: 1.943 ± 0.052	1.53GlnGln: 1.53 ± 0.04	3.341GlnArg: 3.341 ± 0.077	1.887GlnSer: 1.887 ± 0.046	1.815GlnThr: 1.815 ± 0.049	2.388GlnVal: 2.388 ± 0.053	0.537GlnTrp: 0.537 ± 0.024	0.799GlnTyr: 0.799 ± 0.034	0.0GlnXaa: 0.0 ± 0.0
Arg	7.389ArgAla: 7.389 ± 0.094	0.848ArgCys: 0.848 ± 0.036	4.219ArgAsp: 4.219 ± 0.071	4.765ArgGlu: 4.765 ± 0.083	3.355ArgPhe: 3.355 ± 0.059	4.925ArgGly: 4.925 ± 0.084	2.049ArgHis: 2.049 ± 0.049	4.116ArgIle: 4.116 ± 0.075	2.257ArgLys: 2.257 ± 0.053	9.367ArgLeu: 9.367 ± 0.119	1.698ArgMet: 1.698 ± 0.036	2.146ArgAsn: 2.146 ± 0.047	3.532ArgPro: 3.532 ± 0.062	3.103ArgGln: 3.103 ± 0.054	6.346ArgArg: 6.346 ± 0.105	4.253ArgSer: 4.253 ± 0.077	3.557ArgThr: 3.557 ± 0.057	5.57ArgVal: 5.57 ± 0.075	1.386ArgTrp: 1.386 ± 0.039	2.401ArgTyr: 2.401 ± 0.055	0.0ArgXaa: 0.0 ± 0.0
Ser	6.226SerAla: 6.226 ± 0.098	0.64SerCys: 0.64 ± 0.028	3.039SerAsp: 3.039 ± 0.069	3.048SerGlu: 3.048 ± 0.062	2.118SerPhe: 2.118 ± 0.044	5.504SerGly: 5.504 ± 0.094	1.285SerHis: 1.285 ± 0.037	2.418SerIle: 2.418 ± 0.053	1.379SerLys: 1.379 ± 0.043	6.048SerLeu: 6.048 ± 0.093	1.035SerMet: 1.035 ± 0.034	1.378SerAsn: 1.378 ± 0.042	3.029SerPro: 3.029 ± 0.056	1.766SerGln: 1.766 ± 0.045	4.172SerArg: 4.172 ± 0.077	3.062SerSer: 3.062 ± 0.072	2.608SerThr: 2.608 ± 0.059	4.109SerVal: 4.109 ± 0.074	0.791SerTrp: 0.791 ± 0.027	1.295SerTyr: 1.295 ± 0.04	0.0SerXaa: 0.0 ± 0.0
Thr	6.042ThrAla: 6.042 ± 0.082	0.43ThrCys: 0.43 ± 0.022	2.719ThrAsp: 2.719 ± 0.062	2.881ThrGlu: 2.881 ± 0.056	1.87ThrPhe: 1.87 ± 0.048	4.824ThrGly: 4.824 ± 0.078	1.193ThrHis: 1.193 ± 0.034	2.361ThrIle: 2.361 ± 0.047	1.164ThrLys: 1.164 ± 0.035	6.255ThrLeu: 6.255 ± 0.086	0.919ThrMet: 0.919 ± 0.032	1.26ThrAsn: 1.26 ± 0.047	3.292ThrPro: 3.292 ± 0.057	1.621ThrGln: 1.621 ± 0.045	3.536ThrArg: 3.536 ± 0.067	2.502ThrSer: 2.502 ± 0.059	2.563ThrThr: 2.563 ± 0.062	4.255ThrVal: 4.255 ± 0.075	0.675ThrTrp: 0.675 ± 0.03	1.197ThrTyr: 1.197 ± 0.037	0.0ThrXaa: 0.0 ± 0.0
Val	8.371ValAla: 8.371 ± 0.106	0.79ValCys: 0.79 ± 0.029	4.327ValAsp: 4.327 ± 0.07	4.31ValGlu: 4.31 ± 0.078	2.974ValPhe: 2.974 ± 0.062	5.482ValGly: 5.482 ± 0.081	1.576ValHis: 1.576 ± 0.04	3.82ValIle: 3.82 ± 0.069	2.082ValLys: 2.082 ± 0.061	7.824ValLeu: 7.824 ± 0.11	1.661ValMet: 1.661 ± 0.044	2.091ValAsn: 2.091 ± 0.049	3.785ValPro: 3.785 ± 0.058	2.21ValGln: 2.21 ± 0.041	5.134ValArg: 5.134 ± 0.077	4.196ValSer: 4.196 ± 0.077	4.223ValThr: 4.223 ± 0.077	5.618ValVal: 5.618 ± 0.089	0.97ValTrp: 0.97 ± 0.037	1.742ValTyr: 1.742 ± 0.042	0.0ValXaa: 0.0 ± 0.0
Trp	1.244TrpAla: 1.244 ± 0.037	0.146TrpCys: 0.146 ± 0.013	0.762TrpAsp: 0.762 ± 0.029	0.716TrpGlu: 0.716 ± 0.03	0.606TrpPhe: 0.606 ± 0.027	0.965TrpGly: 0.965 ± 0.034	0.395TrpHis: 0.395 ± 0.021	0.729TrpIle: 0.729 ± 0.026	0.453TrpLys: 0.453 ± 0.024	2.143TrpLeu: 2.143 ± 0.056	0.304TrpMet: 0.304 ± 0.016	0.476TrpAsn: 0.476 ± 0.022	0.693TrpPro: 0.693 ± 0.028	0.682TrpGln: 0.682 ± 0.031	1.227TrpArg: 1.227 ± 0.04	0.918TrpSer: 0.918 ± 0.034	0.709TrpThr: 0.709 ± 0.029	1.124TrpVal: 1.124 ± 0.033	0.247TrpTrp: 0.247 ± 0.018	0.349TrpTyr: 0.349 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.621TyrAla: 2.621 ± 0.054	0.316TyrCys: 0.316 ± 0.019	1.435TyrAsp: 1.435 ± 0.049	1.217TyrGlu: 1.217 ± 0.035	1.096TyrPhe: 1.096 ± 0.035	2.123TyrGly: 2.123 ± 0.049	0.541TyrHis: 0.541 ± 0.024	0.897TyrIle: 0.897 ± 0.029	0.546TyrLys: 0.546 ± 0.026	3.018TyrLeu: 3.018 ± 0.062	0.352TyrMet: 0.352 ± 0.018	0.622TyrAsn: 0.622 ± 0.031	1.348TyrPro: 1.348 ± 0.041	0.983TyrGln: 0.983 ± 0.031	2.606TyrArg: 2.606 ± 0.048	1.23TyrSer: 1.23 ± 0.037	1.179TyrThr: 1.179 ± 0.035	1.804TyrVal: 1.804 ± 0.041	0.431TyrTrp: 0.431 ± 0.02	0.705TyrTyr: 0.705 ± 0.029	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2839 proteins (968889 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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