Faecalibacterium sp. CAG:1138
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1284 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R5FJG9|R5FJG9_9FIRM Abortive infection protein OS=Faecalibacterium sp. CAG:1138 OX=1262896 GN=BN468_00016 PE=4 SV=1
MM1 pKa = 8.01 DD2 pKa = 5.13 GVPTPGNAWGNPLFKK17 pKa = 11.18 SPDD20 pKa = 3.03 TDD22 pKa = 3.95 ANDD25 pKa = 3.47 WTTSADD31 pKa = 3.58 FVIDD35 pKa = 3.41 YY36 pKa = 9.8 VRR38 pKa = 11.84 HH39 pKa = 5.34 YY40 pKa = 10.85 RR41 pKa = 11.84 LDD43 pKa = 4.08 DD44 pKa = 3.45 NTEE47 pKa = 3.84 YY48 pKa = 10.77 FYY50 pKa = 11.86 GDD52 pKa = 3.02
Molecular weight: 5.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.729
IPC2_protein 3.821
IPC_protein 3.757
Toseland 3.528
ProMoST 3.872
Dawson 3.795
Bjellqvist 4.05
Wikipedia 3.808
Rodwell 3.592
Grimsley 3.452
Solomon 3.77
Lehninger 3.719
Nozaki 3.935
DTASelect 4.24
Thurlkill 3.63
EMBOSS 3.808
Sillero 3.897
Patrickios 1.926
IPC_peptide 3.757
IPC2_peptide 3.859
IPC2.peptide.svr19 3.866
Protein with the highest isoelectric point:
>tr|R5FFI1|R5FFI1_9FIRM Pseudouridine synthase OS=Faecalibacterium sp. CAG:1138 OX=1262896 GN=BN468_00270 PE=3 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.67 QPKK8 pKa = 9.11 KK9 pKa = 7.52 RR10 pKa = 11.84 QRR12 pKa = 11.84 SKK14 pKa = 9.07 VHH16 pKa = 5.92 GFRR19 pKa = 11.84 KK20 pKa = 10.03 RR21 pKa = 11.84 MASASGRR28 pKa = 11.84 KK29 pKa = 7.75 VLKK32 pKa = 10.06 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.53 GRR39 pKa = 11.84 AKK41 pKa = 10.69 LSAA44 pKa = 3.92
Molecular weight: 5.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.415
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.427
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.149
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.006
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1284
0
1284
443877
33
6396
345.7
38.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.327 ± 0.071
1.358 ± 0.031
5.917 ± 0.059
7.083 ± 0.072
4.567 ± 0.049
7.427 ± 0.063
1.307 ± 0.03
6.27 ± 0.06
7.993 ± 0.062
8.757 ± 0.093
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.427 ± 0.038
4.399 ± 0.042
3.23 ± 0.04
2.284 ± 0.031
3.913 ± 0.052
6.12 ± 0.054
5.595 ± 0.065
8.297 ± 0.07
0.755 ± 0.019
3.974 ± 0.051
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here