Prevotella sp. ne3005

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; unclassified Prevotella

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3038 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H8FPC1|A0A1H8FPC1_9BACT Uncharacterized protein OS=Prevotella sp. ne3005 OX=1761887 GN=SAMN04487902_1129 PE=4 SV=1
MM1 pKa = 7.2YY2 pKa = 10.58GSGDD6 pKa = 3.34KK7 pKa = 10.59EE8 pKa = 3.7YY9 pKa = 10.71DD10 pKa = 3.4YY11 pKa = 11.28MRR13 pKa = 11.84GIDD16 pKa = 4.0PASEE20 pKa = 4.21DD21 pKa = 4.62DD22 pKa = 4.55MEE24 pKa = 4.45NHH26 pKa = 7.37DD27 pKa = 5.0DD28 pKa = 3.76EE29 pKa = 4.59WWNYY33 pKa = 8.08

Molecular weight: 4.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H8CEP4|A0A1H8CEP4_9BACT DNA-binding protein histone-like putative OS=Prevotella sp. ne3005 OX=1761887 GN=SAMN04487902_10556 PE=4 SV=1
MM1 pKa = 7.85PNGKK5 pKa = 9.19KK6 pKa = 10.25KK7 pKa = 10.12KK8 pKa = 7.0GHH10 pKa = 6.14KK11 pKa = 9.06MATHH15 pKa = 6.13KK16 pKa = 10.39RR17 pKa = 11.84KK18 pKa = 9.84KK19 pKa = 9.28RR20 pKa = 11.84LRR22 pKa = 11.84KK23 pKa = 9.25NRR25 pKa = 11.84HH26 pKa = 4.69KK27 pKa = 11.1SKK29 pKa = 11.1

Molecular weight: 3.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3038

0

3038

1096786

29

2459

361.0

40.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.294 ± 0.042

1.337 ± 0.016

5.949 ± 0.028

6.455 ± 0.042

4.403 ± 0.026

6.951 ± 0.042

2.099 ± 0.023

6.64 ± 0.04

6.365 ± 0.044

8.911 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.055 ± 0.02

4.738 ± 0.033

3.747 ± 0.022

3.866 ± 0.033

4.71 ± 0.034

5.772 ± 0.032

5.505 ± 0.034

6.497 ± 0.034

1.383 ± 0.018

4.319 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski