Papaya leaf curl alphasatellite
Average proteome isoelectric point is 8.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|V9I5Y2|V9I5Y2_9VIRU Rep protein OS=Papaya leaf curl alphasatellite OX=1112202 PE=4 SV=1
MM1 pKa = 6.91 SHH3 pKa = 5.9 SRR5 pKa = 11.84 NWCFTIFNYY14 pKa = 9.97 VLPLFTSLPEE24 pKa = 3.69 WANYY28 pKa = 10.56 LIFQEE33 pKa = 4.94 EE34 pKa = 4.56 EE35 pKa = 4.2 CPTTKK40 pKa = 9.85 KK41 pKa = 10.48 RR42 pKa = 11.84 HH43 pKa = 4.56 IQGYY47 pKa = 9.45 VNLNAINDD55 pKa = 3.67 LRR57 pKa = 11.84 SCKK60 pKa = 10.1 RR61 pKa = 11.84 NCQMGLISKK70 pKa = 9.63 HH71 pKa = 6.19 AGVHH75 pKa = 5.71 HH76 pKa = 6.53 PAIEE80 pKa = 4.6 IIAEE84 pKa = 4.07 RR85 pKa = 11.84 MIQEE89 pKa = 4.37 STVHH93 pKa = 6.17 GSSEE97 pKa = 4.18 SLRR100 pKa = 11.84 KK101 pKa = 9.84 QGATRR106 pKa = 11.84 GRR108 pKa = 11.84 RR109 pKa = 11.84 WNVFRR114 pKa = 11.84 KK115 pKa = 6.43 TQKK118 pKa = 10.89 NLDD121 pKa = 4.23 LPTLNCIVAAWRR133 pKa = 11.84 RR134 pKa = 11.84 RR135 pKa = 11.84 LIRR138 pKa = 11.84 SSVVWYY144 pKa = 10.5 SLYY147 pKa = 10.2 LTDD150 pKa = 6.14 LGSSWLRR157 pKa = 11.84 RR158 pKa = 11.84 YY159 pKa = 6.34 WTRR162 pKa = 11.84 AQMIEE167 pKa = 4.04 LSYY170 pKa = 11.76 GCMALKK176 pKa = 10.47 AMKK179 pKa = 9.93 VKK181 pKa = 10.2 PHH183 pKa = 5.85 GLRR186 pKa = 11.84 PRR188 pKa = 11.84 FSKK191 pKa = 10.84 DD192 pKa = 2.69 GSIQGEE198 pKa = 4.45 EE199 pKa = 4.08 KK200 pKa = 10.43 EE201 pKa = 4.3 KK202 pKa = 10.42 TYY204 pKa = 9.36 NTSMRR209 pKa = 11.84 SIWVTVYY216 pKa = 9.5 STSPDD221 pKa = 2.92 RR222 pKa = 11.84 WKK224 pKa = 9.58 TIYY227 pKa = 10.65 SILYY231 pKa = 7.95 YY232 pKa = 10.36 KK233 pKa = 10.42 KK234 pKa = 10.57 LRR236 pKa = 11.84 IDD238 pKa = 3.5 YY239 pKa = 8.35 WSSKK243 pKa = 10.09 YY244 pKa = 10.7 EE245 pKa = 4.52 PIDD248 pKa = 3.78 FNCSDD253 pKa = 3.55 KK254 pKa = 11.12 VHH256 pKa = 6.12 VVVLSNFLPCLDD268 pKa = 4.26 LEE270 pKa = 4.53 YY271 pKa = 10.87 NNRR274 pKa = 11.84 GEE276 pKa = 4.44 TVKK279 pKa = 10.84 KK280 pKa = 10.05 PLLSRR285 pKa = 11.84 DD286 pKa = 3.15 RR287 pKa = 11.84 VFLINIDD294 pKa = 3.84 EE295 pKa = 4.66 SVCGHH300 pKa = 7.2 PDD302 pKa = 3.41 DD303 pKa = 5.71 LKK305 pKa = 11.51 SFDD308 pKa = 4.36 MYY310 pKa = 11.58 LEE312 pKa = 4.15
Molecular weight: 36.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.82
IPC2_protein 8.77
IPC_protein 8.682
Toseland 9.165
ProMoST 9.121
Dawson 9.531
Bjellqvist 9.399
Wikipedia 9.721
Rodwell 9.692
Grimsley 9.589
Solomon 9.575
Lehninger 9.531
Nozaki 9.502
DTASelect 9.311
Thurlkill 9.399
EMBOSS 9.663
Sillero 9.56
Patrickios 4.546
IPC_peptide 9.56
IPC2_peptide 8.287
IPC2.peptide.svr19 7.785
Protein with the highest isoelectric point:
>tr|V9I5Y2|V9I5Y2_9VIRU Rep protein OS=Papaya leaf curl alphasatellite OX=1112202 PE=4 SV=1
MM1 pKa = 6.91 SHH3 pKa = 5.9 SRR5 pKa = 11.84 NWCFTIFNYY14 pKa = 9.97 VLPLFTSLPEE24 pKa = 3.69 WANYY28 pKa = 10.56 LIFQEE33 pKa = 4.94 EE34 pKa = 4.56 EE35 pKa = 4.2 CPTTKK40 pKa = 9.85 KK41 pKa = 10.48 RR42 pKa = 11.84 HH43 pKa = 4.56 IQGYY47 pKa = 9.45 VNLNAINDD55 pKa = 3.67 LRR57 pKa = 11.84 SCKK60 pKa = 10.1 RR61 pKa = 11.84 NCQMGLISKK70 pKa = 9.63 HH71 pKa = 6.19 AGVHH75 pKa = 5.71 HH76 pKa = 6.53 PAIEE80 pKa = 4.6 IIAEE84 pKa = 4.07 RR85 pKa = 11.84 MIQEE89 pKa = 4.37 STVHH93 pKa = 6.17 GSSEE97 pKa = 4.18 SLRR100 pKa = 11.84 KK101 pKa = 9.84 QGATRR106 pKa = 11.84 GRR108 pKa = 11.84 RR109 pKa = 11.84 WNVFRR114 pKa = 11.84 KK115 pKa = 6.43 TQKK118 pKa = 10.89 NLDD121 pKa = 4.23 LPTLNCIVAAWRR133 pKa = 11.84 RR134 pKa = 11.84 RR135 pKa = 11.84 LIRR138 pKa = 11.84 SSVVWYY144 pKa = 10.5 SLYY147 pKa = 10.2 LTDD150 pKa = 6.14 LGSSWLRR157 pKa = 11.84 RR158 pKa = 11.84 YY159 pKa = 6.34 WTRR162 pKa = 11.84 AQMIEE167 pKa = 4.04 LSYY170 pKa = 11.76 GCMALKK176 pKa = 10.47 AMKK179 pKa = 9.93 VKK181 pKa = 10.2 PHH183 pKa = 5.85 GLRR186 pKa = 11.84 PRR188 pKa = 11.84 FSKK191 pKa = 10.84 DD192 pKa = 2.69 GSIQGEE198 pKa = 4.45 EE199 pKa = 4.08 KK200 pKa = 10.43 EE201 pKa = 4.3 KK202 pKa = 10.42 TYY204 pKa = 9.36 NTSMRR209 pKa = 11.84 SIWVTVYY216 pKa = 9.5 STSPDD221 pKa = 2.92 RR222 pKa = 11.84 WKK224 pKa = 9.58 TIYY227 pKa = 10.65 SILYY231 pKa = 7.95 YY232 pKa = 10.36 KK233 pKa = 10.42 KK234 pKa = 10.57 LRR236 pKa = 11.84 IDD238 pKa = 3.5 YY239 pKa = 8.35 WSSKK243 pKa = 10.09 YY244 pKa = 10.7 EE245 pKa = 4.52 PIDD248 pKa = 3.78 FNCSDD253 pKa = 3.55 KK254 pKa = 11.12 VHH256 pKa = 6.12 VVVLSNFLPCLDD268 pKa = 4.26 LEE270 pKa = 4.53 YY271 pKa = 10.87 NNRR274 pKa = 11.84 GEE276 pKa = 4.44 TVKK279 pKa = 10.84 KK280 pKa = 10.05 PLLSRR285 pKa = 11.84 DD286 pKa = 3.15 RR287 pKa = 11.84 VFLINIDD294 pKa = 3.84 EE295 pKa = 4.66 SVCGHH300 pKa = 7.2 PDD302 pKa = 3.41 DD303 pKa = 5.71 LKK305 pKa = 11.51 SFDD308 pKa = 4.36 MYY310 pKa = 11.58 LEE312 pKa = 4.15
Molecular weight: 36.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.82
IPC2_protein 8.77
IPC_protein 8.682
Toseland 9.165
ProMoST 9.121
Dawson 9.531
Bjellqvist 9.399
Wikipedia 9.721
Rodwell 9.692
Grimsley 9.589
Solomon 9.575
Lehninger 9.531
Nozaki 9.502
DTASelect 9.311
Thurlkill 9.399
EMBOSS 9.663
Sillero 9.56
Patrickios 4.546
IPC_peptide 9.56
IPC2_peptide 8.287
IPC2.peptide.svr19 7.785
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
312
312
312
312.0
36.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.526 ± 0.0
2.885 ± 0.0
4.487 ± 0.0
5.449 ± 0.0
3.205 ± 0.0
4.167 ± 0.0
2.885 ± 0.0
6.41 ± 0.0
6.731 ± 0.0
9.615 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.564 ± 0.0
5.128 ± 0.0
3.846 ± 0.0
2.564 ± 0.0
8.013 ± 0.0
9.295 ± 0.0
5.128 ± 0.0
5.769 ± 0.0
3.205 ± 0.0
5.128 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here