Lactococcus phage TP901-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q38183|Q38183_9CAUD Bacteriophage TP901-1 ORF1 2 & 3 OS=Lactococcus phage TP901-1 OX=35345 PE=4 SV=1
MM1 pKa = 7.48SKK3 pKa = 10.29LRR5 pKa = 11.84EE6 pKa = 3.92LSRR9 pKa = 11.84EE10 pKa = 3.8LGAEE14 pKa = 4.09IIYY17 pKa = 7.59FIPSEE22 pKa = 4.0NDD24 pKa = 2.53IALIDD29 pKa = 4.96DD30 pKa = 3.84IKK32 pKa = 11.3GLYY35 pKa = 9.19LPEE38 pKa = 4.07YY39 pKa = 9.78DD40 pKa = 4.25IIYY43 pKa = 10.32IRR45 pKa = 11.84DD46 pKa = 3.81DD47 pKa = 3.33LTIIEE52 pKa = 4.18QEE54 pKa = 3.93NVILHH59 pKa = 5.97EE60 pKa = 5.47LGHH63 pKa = 6.59CYY65 pKa = 10.07CGHH68 pKa = 4.87THH70 pKa = 5.93YY71 pKa = 10.79NCHH74 pKa = 5.87SKK76 pKa = 9.75MFGSKK81 pKa = 9.71QEE83 pKa = 3.99AQADD87 pKa = 3.76RR88 pKa = 11.84FMVVHH93 pKa = 7.16RR94 pKa = 11.84FNEE97 pKa = 4.37WLSKK101 pKa = 10.04WDD103 pKa = 4.01FAPEE107 pKa = 3.82PNEE110 pKa = 3.72INISQFMDD118 pKa = 3.51AYY120 pKa = 10.33EE121 pKa = 4.58LNNKK125 pKa = 8.64LKK127 pKa = 9.84WICEE131 pKa = 4.09LVIEE135 pKa = 4.99EE136 pKa = 4.27YY137 pKa = 9.64TAEE140 pKa = 3.98YY141 pKa = 10.54HH142 pKa = 6.15EE143 pKa = 5.56AII145 pKa = 4.72

Molecular weight: 17.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9AZ72|Q9AZ72_9CAUD ORF25 OS=Lactococcus phage TP901-1 OX=35345 PE=4 SV=1
MM1 pKa = 7.69KK2 pKa = 10.39LLCKK6 pKa = 10.53LFGHH10 pKa = 6.98KK11 pKa = 9.53WDD13 pKa = 3.79DD14 pKa = 3.04WKK16 pKa = 11.36KK17 pKa = 9.22RR18 pKa = 11.84VPNASYY24 pKa = 10.18EE25 pKa = 3.62IRR27 pKa = 11.84ICYY30 pKa = 9.56RR31 pKa = 11.84CGHH34 pKa = 5.95RR35 pKa = 11.84EE36 pKa = 3.42RR37 pKa = 11.84RR38 pKa = 11.84LPTNYY43 pKa = 10.05HH44 pKa = 5.53KK45 pKa = 10.46WLDD48 pKa = 3.46KK49 pKa = 11.55HH50 pKa = 6.11MDD52 pKa = 3.19

Molecular weight: 6.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

11581

47

937

206.8

23.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.442 ± 0.442

0.518 ± 0.094

6.364 ± 0.181

7.262 ± 0.568

4.343 ± 0.198

6.148 ± 0.493

1.131 ± 0.116

7.037 ± 0.307

9.075 ± 0.409

8.298 ± 0.354

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.582 ± 0.141

6.26 ± 0.224

2.988 ± 0.154

4.153 ± 0.206

3.402 ± 0.26

6.882 ± 0.324

6.07 ± 0.318

5.923 ± 0.259

1.338 ± 0.179

3.782 ± 0.268
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski