Ruminiclostridium sufflavum DSM 19573

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Ruminiclostridium; Ruminiclostridium sufflavum

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3674 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A318XSA3|A0A318XSA3_9FIRM N-acylneuraminate cytidylyltransferase OS=Ruminiclostridium sufflavum DSM 19573 OX=1121337 GN=LY28_00944 PE=4 SV=1
MM1 pKa = 7.47LKK3 pKa = 10.03KK4 pKa = 10.4SKK6 pKa = 10.64SILFIILITIALIPSITFVQAVEE29 pKa = 4.79PIQGGTSINNAALISKK45 pKa = 8.38TDD47 pKa = 3.54SLISITGDD55 pKa = 3.12SINPVNAYY63 pKa = 9.86FKK65 pKa = 10.64FVAATTGNFTITTSPVSADD84 pKa = 3.63TEE86 pKa = 4.05AMIYY90 pKa = 10.47YY91 pKa = 7.77DD92 pKa = 3.47TSEE95 pKa = 4.22TSGLQAIGDD104 pKa = 3.83NTDD107 pKa = 4.25CEE109 pKa = 5.07FLLQDD114 pKa = 4.6NLNEE118 pKa = 3.99GQIYY122 pKa = 8.69YY123 pKa = 10.63LKK125 pKa = 10.03IWEE128 pKa = 4.35YY129 pKa = 11.29ANGNAEE135 pKa = 3.91VTINITGGLLMAEE148 pKa = 4.27PEE150 pKa = 3.99ITITNSAANLSYY162 pKa = 10.38MGGQKK167 pKa = 9.79ISISGTVFDD176 pKa = 4.88AEE178 pKa = 4.91GYY180 pKa = 10.25DD181 pKa = 3.53VTVSATIDD189 pKa = 3.2GHH191 pKa = 6.52LVSKK195 pKa = 9.02TVSGGAGAWTLEE207 pKa = 3.59WDD209 pKa = 3.66IDD211 pKa = 4.43SMNISDD217 pKa = 4.87NKK219 pKa = 8.07YY220 pKa = 9.74QNIEE224 pKa = 4.47FIVDD228 pKa = 3.37DD229 pKa = 4.4GTPVGPVSAIHH240 pKa = 6.28TGDD243 pKa = 3.06IVVDD247 pKa = 3.81KK248 pKa = 9.59TPPPAPTAVTVTPVGGMVIANSLNKK273 pKa = 10.47SNTNMTATATITAAEE288 pKa = 4.04AAGGKK293 pKa = 10.13AEE295 pKa = 4.68LYY297 pKa = 10.79LDD299 pKa = 4.1GVLLATDD306 pKa = 3.59NSIASEE312 pKa = 4.06DD313 pKa = 3.88TQVTFDD319 pKa = 4.54LGKK322 pKa = 9.01STNAEE327 pKa = 3.63LQAAVVSSGTVSVKK341 pKa = 10.74LYY343 pKa = 10.54DD344 pKa = 3.52AAGNSSTSSVNNPVLAVDD362 pKa = 3.9YY363 pKa = 10.49NISEE367 pKa = 4.39QVPPTATVYY376 pKa = 10.58TIGGTLRR383 pKa = 11.84VGGTLTGSYY392 pKa = 9.53TYY394 pKa = 11.22YY395 pKa = 10.75DD396 pKa = 3.59ANGDD400 pKa = 3.82SEE402 pKa = 5.08GISTFKK408 pKa = 10.33WYY410 pKa = 8.52TADD413 pKa = 5.45DD414 pKa = 3.74SSGTNKK420 pKa = 9.78TEE422 pKa = 3.73IAGANAEE429 pKa = 4.46SYY431 pKa = 11.15VLTSADD437 pKa = 2.83IGKK440 pKa = 10.27YY441 pKa = 9.68ISFEE445 pKa = 4.29VKK447 pKa = 9.93PVALTGTTQGTAA459 pKa = 3.01

Molecular weight: 47.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A318Y9S7|A0A318Y9S7_9FIRM ATP-binding cassette subfamily F protein uup OS=Ruminiclostridium sufflavum DSM 19573 OX=1121337 GN=LY28_00966 PE=4 SV=1
MM1 pKa = 7.45LRR3 pKa = 11.84TYY5 pKa = 9.82QPKK8 pKa = 9.61KK9 pKa = 8.53RR10 pKa = 11.84HH11 pKa = 5.03RR12 pKa = 11.84QKK14 pKa = 10.33EE15 pKa = 3.73HH16 pKa = 5.99GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTSNGRR28 pKa = 11.84KK29 pKa = 7.2VLRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.24GRR39 pKa = 11.84KK40 pKa = 9.08AISAA44 pKa = 3.87

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3674

0

3674

1258753

39

6533

342.6

38.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.166 ± 0.047

1.36 ± 0.018

5.518 ± 0.034

6.924 ± 0.043

4.252 ± 0.035

6.845 ± 0.043

1.401 ± 0.017

8.691 ± 0.049

7.627 ± 0.042

8.836 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.632 ± 0.021

5.355 ± 0.034

3.153 ± 0.046

2.85 ± 0.022

3.886 ± 0.03

6.788 ± 0.033

5.223 ± 0.048

6.495 ± 0.039

0.862 ± 0.016

4.137 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski