Mycobacterium phage Whouxphf

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 108 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G3M1Y0|A0A3G3M1Y0_9CAUD Integrase OS=Mycobacterium phage Whouxphf OX=2484216 GN=42 PE=3 SV=1
MM1 pKa = 7.44TCLLCDD7 pKa = 4.01HH8 pKa = 7.18PRR10 pKa = 11.84STHH13 pKa = 5.11TPQCRR18 pKa = 11.84TRR20 pKa = 11.84LGVDD24 pKa = 2.95ADD26 pKa = 3.66DD27 pKa = 3.59MTRR30 pKa = 11.84YY31 pKa = 6.71TQCLCPGFEE40 pKa = 4.04GTEE43 pKa = 4.06DD44 pKa = 3.73EE45 pKa = 5.32EE46 pKa = 4.61EE47 pKa = 4.27DD48 pKa = 3.5

Molecular weight: 5.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G3M2V0|A0A3G3M2V0_9CAUD Uncharacterized protein OS=Mycobacterium phage Whouxphf OX=2484216 GN=88 PE=4 SV=1
MM1 pKa = 7.5SDD3 pKa = 3.04VVEE6 pKa = 4.15RR7 pKa = 11.84AKK9 pKa = 10.86AALVDD14 pKa = 3.76YY15 pKa = 9.81EE16 pKa = 4.33VAKK19 pKa = 10.43GSRR22 pKa = 11.84VAVAPGRR29 pKa = 11.84SYY31 pKa = 11.45RR32 pKa = 11.84LLAEE36 pKa = 4.4LVAEE40 pKa = 4.52VEE42 pKa = 4.3RR43 pKa = 11.84LRR45 pKa = 11.84PRR47 pKa = 11.84GLRR50 pKa = 11.84LLLISNGSQRR60 pKa = 11.84IPEE63 pKa = 4.7IIRR66 pKa = 11.84GG67 pKa = 3.66

Molecular weight: 7.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

108

0

108

19003

25

1210

176.0

19.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.304 ± 0.471

1.31 ± 0.176

6.583 ± 0.254

6.031 ± 0.313

2.926 ± 0.179

8.872 ± 0.647

2.342 ± 0.167

4.347 ± 0.198

3.452 ± 0.206

7.183 ± 0.195

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.284 ± 0.131

3.347 ± 0.158

6.199 ± 0.186

3.494 ± 0.169

6.836 ± 0.351

6.083 ± 0.253

6.546 ± 0.278

7.178 ± 0.238

2.326 ± 0.15

2.358 ± 0.155
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski