Aliifodinibius halophilus

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Balneolaeota; Balneolia; Balneolales; Balneolaceae; Aliifodinibius

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3590 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M1T1B1|A0A6M1T1B1_9BACT Indole-3-glycerol phosphate synthase OS=Aliifodinibius halophilus OX=1736908 GN=trpC PE=3 SV=1
MM1 pKa = 7.47KK2 pKa = 10.65LSFTKK7 pKa = 10.48VASALVLALTVIVTGCNDD25 pKa = 4.41DD26 pKa = 4.31GPNGPDD32 pKa = 3.41SNPLSVEE39 pKa = 4.41SINPTAGQPGDD50 pKa = 3.61EE51 pKa = 4.25VTITGTGFANNADD64 pKa = 4.1DD65 pKa = 5.01NSVTFAGDD73 pKa = 3.14VTASISSVSATEE85 pKa = 3.99LVVTVPQGATTGPITVSVDD104 pKa = 3.16GEE106 pKa = 4.41SATTSEE112 pKa = 4.63FAISSEE118 pKa = 4.03PQPEE122 pKa = 4.01IPEE125 pKa = 4.63PYY127 pKa = 10.5ASFPGTKK134 pKa = 9.11IAADD138 pKa = 3.96EE139 pKa = 4.28TWSNDD144 pKa = 2.94TTLVGPHH151 pKa = 5.95FVLPGVTLTVEE162 pKa = 4.2AGVEE166 pKa = 4.23VTFEE170 pKa = 3.98YY171 pKa = 11.07HH172 pKa = 6.48NGNDD176 pKa = 3.81DD177 pKa = 3.69KK178 pKa = 11.79VGAIYY183 pKa = 9.84TLASDD188 pKa = 4.26DD189 pKa = 4.02QNFSEE194 pKa = 4.41PRR196 pKa = 11.84EE197 pKa = 4.14TAKK200 pKa = 10.84LVAEE204 pKa = 4.95GTADD208 pKa = 3.42QPIVFTSDD216 pKa = 2.62RR217 pKa = 11.84KK218 pKa = 10.5QINSWGGIIMTGRR231 pKa = 11.84ASNNIPGGQGTVEE244 pKa = 5.02GIEE247 pKa = 3.63QDD249 pKa = 3.02IFYY252 pKa = 10.86GADD255 pKa = 3.18ADD257 pKa = 5.1GNFNDD262 pKa = 4.63SDD264 pKa = 4.18DD265 pKa = 4.34SGSLEE270 pKa = 4.03YY271 pKa = 11.15VRR273 pKa = 11.84IEE275 pKa = 3.8YY276 pKa = 10.48AGYY279 pKa = 9.92SINAGSEE286 pKa = 4.21LQSLTLYY293 pKa = 10.92SIGSEE298 pKa = 4.09TNINHH303 pKa = 5.52VNLFGSSDD311 pKa = 3.57DD312 pKa = 4.36GIEE315 pKa = 4.15LFGGTVDD322 pKa = 3.05IKK324 pKa = 11.11YY325 pKa = 10.12LVVYY329 pKa = 10.02GADD332 pKa = 4.11DD333 pKa = 4.4DD334 pKa = 5.52SFDD337 pKa = 4.69YY338 pKa = 11.19DD339 pKa = 3.78QGWSGKK345 pKa = 7.89GQFWLAVQRR354 pKa = 11.84DD355 pKa = 3.84GADD358 pKa = 2.9QGFEE362 pKa = 3.97NDD364 pKa = 3.56GCADD368 pKa = 4.62EE369 pKa = 5.55ADD371 pKa = 4.47CDD373 pKa = 3.82QGSGPGSPNISNATVIAPKK392 pKa = 9.15NTASGKK398 pKa = 10.64DD399 pKa = 3.45NMGLKK404 pKa = 10.17LRR406 pKa = 11.84EE407 pKa = 4.27GLQGAYY413 pKa = 10.46RR414 pKa = 11.84NIIVSNFVGNPFYY427 pKa = 11.2LDD429 pKa = 3.61GEE431 pKa = 4.48NNANDD436 pKa = 3.97DD437 pKa = 3.7TYY439 pKa = 11.79EE440 pKa = 4.42NYY442 pKa = 10.64DD443 pKa = 3.34SGDD446 pKa = 3.41LVLEE450 pKa = 4.15NFVLQNNGPWASSDD464 pKa = 3.16EE465 pKa = 4.23SRR467 pKa = 11.84YY468 pKa = 9.47SAVYY472 pKa = 9.98EE473 pKa = 4.11YY474 pKa = 10.76TSSDD478 pKa = 3.24KK479 pKa = 11.09PADD482 pKa = 3.71LFNNPSNYY490 pKa = 10.11DD491 pKa = 3.24YY492 pKa = 11.34SLPSGSAYY500 pKa = 10.66LSGGQAPSDD509 pKa = 3.31SWFDD513 pKa = 3.54DD514 pKa = 3.39VSFRR518 pKa = 11.84GAVGEE523 pKa = 6.14SGTQWDD529 pKa = 4.3WVNEE533 pKa = 4.69GIWVKK538 pKa = 10.39WPII541 pKa = 3.56

Molecular weight: 57.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M1TIF2|A0A6M1TIF2_9BACT UvrABC system protein C OS=Aliifodinibius halophilus OX=1736908 GN=uvrC PE=3 SV=1
PP1 pKa = 7.45AAEE4 pKa = 3.87QSLRR8 pKa = 11.84QCLEE12 pKa = 3.78TLGQQIDD19 pKa = 3.52QLTRR23 pKa = 11.84LIRR26 pKa = 11.84KK27 pKa = 8.09HH28 pKa = 5.26LRR30 pKa = 11.84SRR32 pKa = 11.84EE33 pKa = 3.89EE34 pKa = 3.52LRR36 pKa = 11.84EE37 pKa = 4.09SIKK40 pKa = 10.68YY41 pKa = 10.11LSSIPGIGILTIAVVLAEE59 pKa = 4.24TTGFQQFHH67 pKa = 7.48KK68 pKa = 9.89ISQLISFSGYY78 pKa = 10.63DD79 pKa = 3.28VIIRR83 pKa = 11.84QSGKK87 pKa = 8.45WAGKK91 pKa = 9.25PRR93 pKa = 11.84ISKK96 pKa = 10.19QGSKK100 pKa = 10.49YY101 pKa = 9.6IRR103 pKa = 11.84RR104 pKa = 11.84AMFMPASAVVRR115 pKa = 11.84SGTGPTYY122 pKa = 10.79RR123 pKa = 11.84LYY125 pKa = 11.21

Molecular weight: 14.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3590

0

3590

1192668

21

3099

332.2

37.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.742 ± 0.041

0.667 ± 0.012

6.138 ± 0.039

7.352 ± 0.052

4.493 ± 0.031

6.911 ± 0.043

2.007 ± 0.019

6.978 ± 0.039

6.248 ± 0.042

9.192 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.314 ± 0.024

4.966 ± 0.042

3.866 ± 0.024

4.047 ± 0.027

4.39 ± 0.034

6.835 ± 0.044

5.648 ± 0.046

6.252 ± 0.03

1.236 ± 0.016

3.718 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski