Amino acid dipepetide frequency for Firmicutes bacterium CAG:449

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	1.92AlaAla: 1.92 ± 0.09	0.871AlaCys: 0.871 ± 0.045	2.086AlaAsp: 2.086 ± 0.066	2.178AlaGlu: 2.178 ± 0.08	2.377AlaPhe: 2.377 ± 0.072	2.627AlaGly: 2.627 ± 0.078	0.805AlaHis: 0.805 ± 0.04	4.594AlaIle: 4.594 ± 0.121	4.363AlaLys: 4.363 ± 0.092	5.348AlaLeu: 5.348 ± 0.123	1.101AlaMet: 1.101 ± 0.05	2.823AlaAsn: 2.823 ± 0.077	1.101AlaPro: 1.101 ± 0.04	1.21AlaGln: 1.21 ± 0.057	1.604AlaArg: 1.604 ± 0.057	4.047AlaSer: 4.047 ± 0.091	2.61AlaThr: 2.61 ± 0.075	2.247AlaVal: 2.247 ± 0.068	0.31AlaTrp: 0.31 ± 0.026	2.193AlaTyr: 2.193 ± 0.073	0.012AlaXaa: 0.012 ± 0.005
Cys	0.63CysAla: 0.63 ± 0.037	0.237CysCys: 0.237 ± 0.029	0.828CysAsp: 0.828 ± 0.044	0.785CysGlu: 0.785 ± 0.039	0.861CysPhe: 0.861 ± 0.044	1.125CysGly: 1.125 ± 0.052	0.262CysHis: 0.262 ± 0.024	1.053CysIle: 1.053 ± 0.048	1.154CysLys: 1.154 ± 0.054	1.576CysLeu: 1.576 ± 0.061	0.217CysMet: 0.217 ± 0.024	0.768CysAsn: 0.768 ± 0.041	0.473CysPro: 0.473 ± 0.038	0.304CysGln: 0.304 ± 0.026	0.378CysArg: 0.378 ± 0.026	1.001CysSer: 1.001 ± 0.045	0.504CysThr: 0.504 ± 0.038	0.768CysVal: 0.768 ± 0.038	0.105CysTrp: 0.105 ± 0.014	0.71CysTyr: 0.71 ± 0.035	0.0CysXaa: 0.0 ± 0.0
Asp	2.591AspAla: 2.591 ± 0.07	0.646AspCys: 0.646 ± 0.035	3.589AspAsp: 3.589 ± 0.103	5.224AspGlu: 5.224 ± 0.104	3.075AspPhe: 3.075 ± 0.073	3.484AspGly: 3.484 ± 0.094	0.762AspHis: 0.762 ± 0.045	5.255AspIle: 5.255 ± 0.108	4.865AspLys: 4.865 ± 0.102	5.148AspLeu: 5.148 ± 0.101	1.152AspMet: 1.152 ± 0.045	4.378AspAsn: 4.378 ± 0.091	1.369AspPro: 1.369 ± 0.045	1.274AspGln: 1.274 ± 0.045	1.392AspArg: 1.392 ± 0.052	3.325AspSer: 3.325 ± 0.089	2.337AspThr: 2.337 ± 0.079	3.667AspVal: 3.667 ± 0.085	0.415AspTrp: 0.415 ± 0.037	3.674AspTyr: 3.674 ± 0.091	0.002AspXaa: 0.002 ± 0.002
Glu	3.294GluAla: 3.294 ± 0.096	0.743GluCys: 0.743 ± 0.039	3.779GluAsp: 3.779 ± 0.083	6.122GluGlu: 6.122 ± 0.14	3.066GluPhe: 3.066 ± 0.071	3.289GluGly: 3.289 ± 0.083	0.898GluHis: 0.898 ± 0.042	6.696GluIle: 6.696 ± 0.13	7.256GluLys: 7.256 ± 0.138	5.811GluLeu: 5.811 ± 0.102	1.675GluMet: 1.675 ± 0.056	5.955GluAsn: 5.955 ± 0.113	1.173GluPro: 1.173 ± 0.051	1.701GluGln: 1.701 ± 0.064	1.714GluArg: 1.714 ± 0.06	3.058GluSer: 3.058 ± 0.081	3.114GluThr: 3.114 ± 0.086	4.332GluVal: 4.332 ± 0.093	0.396GluTrp: 0.396 ± 0.027	3.579GluTyr: 3.579 ± 0.093	0.002GluXaa: 0.002 ± 0.002
Phe	2.633PheAla: 2.633 ± 0.071	0.636PheCys: 0.636 ± 0.032	3.463PheAsp: 3.463 ± 0.083	3.06PheGlu: 3.06 ± 0.081	2.608PhePhe: 2.608 ± 0.095	2.532PheGly: 2.532 ± 0.07	0.628PheHis: 0.628 ± 0.034	5.069PheIle: 5.069 ± 0.113	4.151PheLys: 4.151 ± 0.093	4.636PheLeu: 4.636 ± 0.104	1.03PheMet: 1.03 ± 0.047	3.839PheAsn: 3.839 ± 0.087	1.161PhePro: 1.161 ± 0.042	1.032PheGln: 1.032 ± 0.044	1.084PheArg: 1.084 ± 0.052	4.159PheSer: 4.159 ± 0.097	2.911PheThr: 2.911 ± 0.082	3.116PheVal: 3.116 ± 0.074	0.31PheTrp: 0.31 ± 0.029	2.62PheTyr: 2.62 ± 0.076	0.004PheXaa: 0.004 ± 0.003
Gly	2.792GlyAla: 2.792 ± 0.092	0.867GlyCys: 0.867 ± 0.042	2.862GlyAsp: 2.862 ± 0.084	3.455GlyGlu: 3.455 ± 0.084	2.837GlyPhe: 2.837 ± 0.08	3.358GlyGly: 3.358 ± 0.095	0.991GlyHis: 0.991 ± 0.044	5.243GlyIle: 5.243 ± 0.103	4.696GlyLys: 4.696 ± 0.096	4.78GlyLeu: 4.78 ± 0.111	1.185GlyMet: 1.185 ± 0.045	3.192GlyAsn: 3.192 ± 0.091	1.008GlyPro: 1.008 ± 0.054	1.392GlyGln: 1.392 ± 0.054	1.671GlyArg: 1.671 ± 0.055	3.366GlySer: 3.366 ± 0.092	3.093GlyThr: 3.093 ± 0.094	3.603GlyVal: 3.603 ± 0.101	0.514GlyTrp: 0.514 ± 0.031	2.992GlyTyr: 2.992 ± 0.084	0.017GlyXaa: 0.017 ± 0.005
His	0.83HisAla: 0.83 ± 0.039	0.227HisCys: 0.227 ± 0.02	0.814HisAsp: 0.814 ± 0.047	0.861HisGlu: 0.861 ± 0.041	0.894HisPhe: 0.894 ± 0.04	0.929HisGly: 0.929 ± 0.042	0.415HisHis: 0.415 ± 0.035	1.305HisIle: 1.305 ± 0.057	0.956HisLys: 0.956 ± 0.043	1.592HisLeu: 1.592 ± 0.057	0.268HisMet: 0.268 ± 0.022	0.964HisAsn: 0.964 ± 0.05	0.675HisPro: 0.675 ± 0.041	0.646HisGln: 0.646 ± 0.034	0.442HisArg: 0.442 ± 0.025	0.871HisSer: 0.871 ± 0.042	0.688HisThr: 0.688 ± 0.036	0.834HisVal: 0.834 ± 0.037	0.111HisTrp: 0.111 ± 0.015	0.956HisTyr: 0.956 ± 0.045	0.008HisXaa: 0.008 ± 0.005
Ile	4.805IleAla: 4.805 ± 0.092	1.435IleCys: 1.435 ± 0.051	6.089IleAsp: 6.089 ± 0.115	6.036IleGlu: 6.036 ± 0.115	4.596IlePhe: 4.596 ± 0.128	5.154IleGly: 5.154 ± 0.118	1.262IleHis: 1.262 ± 0.058	9.077IleIle: 9.077 ± 0.188	8.582IleLys: 8.582 ± 0.147	8.187IleLeu: 8.187 ± 0.175	1.879IleMet: 1.879 ± 0.057	7.698IleAsn: 7.698 ± 0.134	2.777IlePro: 2.777 ± 0.077	2.03IleGln: 2.03 ± 0.064	2.563IleArg: 2.563 ± 0.069	7.225IleSer: 7.225 ± 0.125	4.941IleThr: 4.941 ± 0.112	5.678IleVal: 5.678 ± 0.111	0.485IleTrp: 0.485 ± 0.032	4.545IleTyr: 4.545 ± 0.11	0.004IleXaa: 0.004 ± 0.003
Lys	4.041LysAla: 4.041 ± 0.108	0.993LysCys: 0.993 ± 0.045	5.904LysAsp: 5.904 ± 0.1	8.082LysGlu: 8.082 ± 0.136	3.304LysPhe: 3.304 ± 0.075	3.992LysGly: 3.992 ± 0.084	1.169LysHis: 1.169 ± 0.046	8.842LysIle: 8.842 ± 0.153	9.525LysLys: 9.525 ± 0.167	7.178LysLeu: 7.178 ± 0.118	2.637LysMet: 2.637 ± 0.08	7.483LysAsn: 7.483 ± 0.137	1.879LysPro: 1.879 ± 0.069	2.689LysGln: 2.689 ± 0.081	2.689LysArg: 2.689 ± 0.078	4.477LysSer: 4.477 ± 0.091	4.545LysThr: 4.545 ± 0.092	5.466LysVal: 5.466 ± 0.11	0.593LysTrp: 0.593 ± 0.032	4.883LysTyr: 4.883 ± 0.095	0.01LysXaa: 0.01 ± 0.004
Leu	4.605LeuAla: 4.605 ± 0.125	1.286LeuCys: 1.286 ± 0.053	5.633LeuAsp: 5.633 ± 0.116	5.687LeuGlu: 5.687 ± 0.108	4.999LeuPhe: 4.999 ± 0.109	4.877LeuGly: 4.877 ± 0.113	1.33LeuHis: 1.33 ± 0.048	9.379LeuIle: 9.379 ± 0.198	8.474LeuLys: 8.474 ± 0.159	9.011LeuLeu: 9.011 ± 0.152	2.187LeuMet: 2.187 ± 0.073	6.837LeuAsn: 6.837 ± 0.125	2.943LeuPro: 2.943 ± 0.083	2.243LeuGln: 2.243 ± 0.076	2.393LeuArg: 2.393 ± 0.074	7.242LeuSer: 7.242 ± 0.129	5.336LeuThr: 5.336 ± 0.114	5.449LeuVal: 5.449 ± 0.094	0.465LeuTrp: 0.465 ± 0.032	3.83LeuTyr: 3.83 ± 0.087	0.017LeuXaa: 0.017 ± 0.005
Met	1.2MetAla: 1.2 ± 0.048	0.279MetCys: 0.279 ± 0.027	1.272MetAsp: 1.272 ± 0.047	1.32MetGlu: 1.32 ± 0.048	1.028MetPhe: 1.028 ± 0.047	1.065MetGly: 1.065 ± 0.049	0.349MetHis: 0.349 ± 0.028	2.117MetIle: 2.117 ± 0.063	2.507MetLys: 2.507 ± 0.066	1.943MetLeu: 1.943 ± 0.068	0.555MetMet: 0.555 ± 0.036	1.642MetAsn: 1.642 ± 0.056	0.739MetPro: 0.739 ± 0.038	0.593MetGln: 0.593 ± 0.034	0.589MetArg: 0.589 ± 0.037	1.412MetSer: 1.412 ± 0.05	0.995MetThr: 0.995 ± 0.043	1.305MetVal: 1.305 ± 0.057	0.161MetTrp: 0.161 ± 0.017	0.89MetTyr: 0.89 ± 0.038	0.004MetXaa: 0.004 ± 0.003
Asn	3.283AsnAla: 3.283 ± 0.083	0.933AsnCys: 0.933 ± 0.049	4.768AsnAsp: 4.768 ± 0.096	5.633AsnGlu: 5.633 ± 0.126	3.347AsnPhe: 3.347 ± 0.085	4.202AsnGly: 4.202 ± 0.095	1.255AsnHis: 1.255 ± 0.061	6.76AsnIle: 6.76 ± 0.143	7.425AsnLys: 7.425 ± 0.132	6.29AsnLeu: 6.29 ± 0.128	1.437AsnMet: 1.437 ± 0.052	6.432AsnAsn: 6.432 ± 0.159	2.077AsnPro: 2.077 ± 0.068	2.482AsnGln: 2.482 ± 0.073	1.881AsnArg: 1.881 ± 0.067	4.21AsnSer: 4.21 ± 0.087	3.093AsnThr: 3.093 ± 0.099	4.291AsnVal: 4.291 ± 0.083	0.5AsnTrp: 0.5 ± 0.034	4.483AsnTyr: 4.483 ± 0.1	0.01AsnXaa: 0.01 ± 0.005
Pro	1.034ProAla: 1.034 ± 0.051	0.365ProCys: 0.365 ± 0.026	1.202ProAsp: 1.202 ± 0.057	1.596ProGlu: 1.596 ± 0.055	1.596ProPhe: 1.596 ± 0.066	1.101ProGly: 1.101 ± 0.053	0.533ProHis: 0.533 ± 0.03	2.179ProIle: 2.179 ± 0.074	1.768ProLys: 1.768 ± 0.062	2.893ProLeu: 2.893 ± 0.081	0.525ProMet: 0.525 ± 0.029	1.71ProAsn: 1.71 ± 0.048	0.44ProPro: 0.44 ± 0.028	0.677ProGln: 0.677 ± 0.031	0.686ProArg: 0.686 ± 0.039	2.274ProSer: 2.274 ± 0.07	1.768ProThr: 1.768 ± 0.06	1.458ProVal: 1.458 ± 0.056	0.206ProTrp: 0.206 ± 0.02	1.547ProTyr: 1.547 ± 0.052	0.006ProXaa: 0.006 ± 0.003
Gln	1.35GlnAla: 1.35 ± 0.052	0.289GlnCys: 0.289 ± 0.024	1.52GlnAsp: 1.52 ± 0.056	2.35GlnGlu: 2.35 ± 0.079	1.148GlnPhe: 1.148 ± 0.042	1.386GlnGly: 1.386 ± 0.05	0.308GlnHis: 0.308 ± 0.022	2.66GlnIle: 2.66 ± 0.074	2.653GlnLys: 2.653 ± 0.074	2.034GlnLeu: 2.034 ± 0.063	0.76GlnMet: 0.76 ± 0.043	2.017GlnAsn: 2.017 ± 0.07	0.493GlnPro: 0.493 ± 0.032	0.811GlnGln: 0.811 ± 0.039	0.847GlnArg: 0.847 ± 0.041	1.165GlnSer: 1.165 ± 0.048	1.555GlnThr: 1.555 ± 0.055	1.567GlnVal: 1.567 ± 0.061	0.178GlnTrp: 0.178 ± 0.02	1.315GlnTyr: 1.315 ± 0.053	0.008GlnXaa: 0.008 ± 0.004
Arg	1.274ArgAla: 1.274 ± 0.045	0.37ArgCys: 0.37 ± 0.029	1.305ArgAsp: 1.305 ± 0.054	1.596ArgGlu: 1.596 ± 0.061	1.384ArgPhe: 1.384 ± 0.051	1.518ArgGly: 1.518 ± 0.055	0.444ArgHis: 0.444 ± 0.031	2.629ArgIle: 2.629 ± 0.073	2.885ArgLys: 2.885 ± 0.079	2.488ArgLeu: 2.488 ± 0.064	0.774ArgMet: 0.774 ± 0.039	2.065ArgAsn: 2.065 ± 0.07	0.716ArgPro: 0.716 ± 0.034	0.739ArgGln: 0.739 ± 0.037	1.039ArgArg: 1.039 ± 0.043	1.474ArgSer: 1.474 ± 0.054	1.284ArgThr: 1.284 ± 0.048	1.716ArgVal: 1.716 ± 0.065	0.209ArgTrp: 0.209 ± 0.019	1.433ArgTyr: 1.433 ± 0.059	0.0ArgXaa: 0.0 ± 0.0
Ser	2.476SerAla: 2.476 ± 0.084	1.051SerCys: 1.051 ± 0.049	3.122SerAsp: 3.122 ± 0.082	3.764SerGlu: 3.764 ± 0.091	4.351SerPhe: 4.351 ± 0.095	3.465SerGly: 3.465 ± 0.087	0.933SerHis: 0.933 ± 0.045	6.442SerIle: 6.442 ± 0.127	6.027SerLys: 6.027 ± 0.11	8.235SerLeu: 8.235 ± 0.161	1.39SerMet: 1.39 ± 0.052	4.912SerAsn: 4.912 ± 0.102	1.538SerPro: 1.538 ± 0.053	1.782SerGln: 1.782 ± 0.07	1.778SerArg: 1.778 ± 0.062	6.777SerSer: 6.777 ± 0.165	3.828SerThr: 3.828 ± 0.103	3.141SerVal: 3.141 ± 0.088	0.45SerTrp: 0.45 ± 0.029	3.977SerTyr: 3.977 ± 0.1	0.014SerXaa: 0.014 ± 0.006
Thr	2.199ThrAla: 2.199 ± 0.077	0.655ThrCys: 0.655 ± 0.034	2.282ThrAsp: 2.282 ± 0.08	2.389ThrGlu: 2.389 ± 0.07	3.023ThrPhe: 3.023 ± 0.099	2.994ThrGly: 2.994 ± 0.099	0.88ThrHis: 0.88 ± 0.045	5.367ThrIle: 5.367 ± 0.113	3.882ThrLys: 3.882 ± 0.087	5.356ThrLeu: 5.356 ± 0.102	1.008ThrMet: 1.008 ± 0.042	3.667ThrAsn: 3.667 ± 0.095	1.765ThrPro: 1.765 ± 0.057	1.115ThrGln: 1.115 ± 0.041	1.408ThrArg: 1.408 ± 0.047	4.722ThrSer: 4.722 ± 0.126	3.153ThrThr: 3.153 ± 0.105	2.523ThrVal: 2.523 ± 0.097	0.471ThrTrp: 0.471 ± 0.033	2.912ThrTyr: 2.912 ± 0.091	0.016ThrXaa: 0.016 ± 0.005
Val	2.711ValAla: 2.711 ± 0.076	0.904ValCys: 0.904 ± 0.041	3.628ValAsp: 3.628 ± 0.081	3.756ValGlu: 3.756 ± 0.103	2.808ValPhe: 2.808 ± 0.075	3.651ValGly: 3.651 ± 0.095	0.781ValHis: 0.781 ± 0.04	5.424ValIle: 5.424 ± 0.126	4.689ValLys: 4.689 ± 0.094	5.383ValLeu: 5.383 ± 0.113	1.074ValMet: 1.074 ± 0.046	4.305ValAsn: 4.305 ± 0.094	1.549ValPro: 1.549 ± 0.054	1.288ValGln: 1.288 ± 0.051	1.526ValArg: 1.526 ± 0.055	4.601ValSer: 4.601 ± 0.094	3.166ValThr: 3.166 ± 0.082	3.568ValVal: 3.568 ± 0.077	0.324ValTrp: 0.324 ± 0.025	2.763ValTyr: 2.763 ± 0.086	0.016ValXaa: 0.016 ± 0.006
Trp	0.351TrpAla: 0.351 ± 0.025	0.118TrpCys: 0.118 ± 0.013	0.368TrpAsp: 0.368 ± 0.024	0.366TrpGlu: 0.366 ± 0.027	0.368TrpPhe: 0.368 ± 0.029	0.401TrpGly: 0.401 ± 0.029	0.114TrpHis: 0.114 ± 0.013	0.543TrpIle: 0.543 ± 0.033	0.516TrpLys: 0.516 ± 0.032	0.555TrpLeu: 0.555 ± 0.033	0.209TrpMet: 0.209 ± 0.02	0.516TrpAsn: 0.516 ± 0.032	0.161TrpPro: 0.161 ± 0.019	0.184TrpGln: 0.184 ± 0.018	0.19TrpArg: 0.19 ± 0.019	0.438TrpSer: 0.438 ± 0.033	0.413TrpThr: 0.413 ± 0.027	0.376TrpVal: 0.376 ± 0.03	0.095TrpTrp: 0.095 ± 0.015	0.357TrpTyr: 0.357 ± 0.032	0.002TrpXaa: 0.002 ± 0.002
Tyr	2.401TyrAla: 2.401 ± 0.078	0.836TyrCys: 0.836 ± 0.042	3.104TyrAsp: 3.104 ± 0.086	3.388TyrGlu: 3.388 ± 0.086	3.006TyrPhe: 3.006 ± 0.084	2.759TyrGly: 2.759 ± 0.083	1.161TyrHis: 1.161 ± 0.052	4.398TyrIle: 4.398 ± 0.105	3.998TyrLys: 3.998 ± 0.104	5.767TyrLeu: 5.767 ± 0.133	0.88TyrMet: 0.88 ± 0.04	3.57TyrAsn: 3.57 ± 0.09	1.528TyrPro: 1.528 ± 0.052	2.22TyrGln: 2.22 ± 0.064	1.458TyrArg: 1.458 ± 0.058	3.601TyrSer: 3.601 ± 0.105	2.439TyrThr: 2.439 ± 0.082	2.804TyrVal: 2.804 ± 0.073	0.343TyrTrp: 0.343 ± 0.026	3.151TyrTyr: 3.151 ± 0.088	0.004TyrXaa: 0.004 ± 0.003
Xaa	0.008XaaAla: 0.008 ± 0.004	0.0XaaCys: 0.0 ± 0.0	0.004XaaAsp: 0.004 ± 0.003	0.004XaaGlu: 0.004 ± 0.003	0.008XaaPhe: 0.008 ± 0.004	0.004XaaGly: 0.004 ± 0.003	0.002XaaHis: 0.002 ± 0.002	0.016XaaIle: 0.016 ± 0.006	0.012XaaLys: 0.012 ± 0.005	0.016XaaLeu: 0.016 ± 0.005	0.019XaaMet: 0.019 ± 0.006	0.017XaaAsn: 0.017 ± 0.006	0.002XaaPro: 0.002 ± 0.002	0.002XaaGln: 0.002 ± 0.002	0.008XaaArg: 0.008 ± 0.004	0.006XaaSer: 0.006 ± 0.003	0.014XaaThr: 0.014 ± 0.005	0.006XaaVal: 0.006 ± 0.003	0.002XaaTrp: 0.002 ± 0.002	0.006XaaTyr: 0.006 ± 0.004	0.186XaaXaa: 0.186 ± 0.026

Statistics based on 1770 proteins (515719 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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