Cedar virus
Average proteome isoelectric point is 6.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|J7GXK9|J7GXK9_9MONO GDP polyribonucleotidyltransferase OS=Cedar virus OX=1221391 GN=L PE=3 SV=1
MM1 pKa = 8.2 DD2 pKa = 5.37 KK3 pKa = 10.94 LQLIEE8 pKa = 5.95 DD9 pKa = 4.16 GLSTINFIQEE19 pKa = 4.0 NKK21 pKa = 10.16 EE22 pKa = 4.07 KK23 pKa = 10.43 LQHH26 pKa = 5.88 SYY28 pKa = 10.56 GRR30 pKa = 11.84 SSIRR34 pKa = 11.84 EE35 pKa = 3.97 PPTSVRR41 pKa = 11.84 VEE43 pKa = 3.82 EE44 pKa = 4.17 WEE46 pKa = 3.95 KK47 pKa = 10.72 FIRR50 pKa = 11.84 KK51 pKa = 8.52 IASGPEE57 pKa = 3.85 QVQGGGSEE65 pKa = 4.28 TEE67 pKa = 3.77 ITGDD71 pKa = 3.21 NGDD74 pKa = 4.06 RR75 pKa = 11.84 GNFTNPDD82 pKa = 3.05 QGGGVTGQFEE92 pKa = 4.1 EE93 pKa = 6.24 RR94 pKa = 11.84 YY95 pKa = 9.42 QKK97 pKa = 10.02 WGSQDD102 pKa = 3.96 SEE104 pKa = 4.43 LQLDD108 pKa = 3.88 PMVVHH113 pKa = 7.27 DD114 pKa = 4.41 FFYY117 pKa = 10.91 DD118 pKa = 3.6 EE119 pKa = 5.0 RR120 pKa = 11.84 RR121 pKa = 11.84 EE122 pKa = 3.94 NPDD125 pKa = 2.74 NGKK128 pKa = 10.11 YY129 pKa = 10.09 DD130 pKa = 3.63 RR131 pKa = 11.84 SSKK134 pKa = 10.47 KK135 pKa = 9.54 RR136 pKa = 11.84 DD137 pKa = 3.57 NIRR140 pKa = 11.84 EE141 pKa = 4.05 GTRR144 pKa = 11.84 QDD146 pKa = 3.19 KK147 pKa = 11.01 YY148 pKa = 11.32 NNQSTDD154 pKa = 3.16 EE155 pKa = 4.7 LLSCLQPSSKK165 pKa = 10.95 NDD167 pKa = 3.36 VIKK170 pKa = 11.14 NEE172 pKa = 4.17 STSVSNLHH180 pKa = 5.11 VTGNKK185 pKa = 9.63 LNPDD189 pKa = 4.05 AKK191 pKa = 10.18 PFEE194 pKa = 4.46 PTSQSKK200 pKa = 7.81 EE201 pKa = 4.08 HH202 pKa = 6.63 PTTTQHH208 pKa = 6.35 NKK210 pKa = 9.71 NDD212 pKa = 3.54 HH213 pKa = 5.47 QTDD216 pKa = 3.41 DD217 pKa = 3.73 DD218 pKa = 4.55 YY219 pKa = 12.04 KK220 pKa = 11.08 NRR222 pKa = 11.84 RR223 pKa = 11.84 SSEE226 pKa = 3.89 NNVISDD232 pKa = 3.79 HH233 pKa = 5.79 ATTMEE238 pKa = 4.83 DD239 pKa = 3.19 NNNFIPATKK248 pKa = 9.99 RR249 pKa = 11.84 KK250 pKa = 9.02 NALSEE255 pKa = 4.36 PIYY258 pKa = 10.7 VQVLPSNTEE267 pKa = 3.88 GFSGKK272 pKa = 10.03 DD273 pKa = 3.36 YY274 pKa = 11.2 PLLKK278 pKa = 10.75 DD279 pKa = 3.4 NSVKK283 pKa = 10.2 KK284 pKa = 10.19 RR285 pKa = 11.84 AEE287 pKa = 3.83 PVILEE292 pKa = 4.5 TANHH296 pKa = 6.67 PAGSADD302 pKa = 3.22 QDD304 pKa = 3.86 TNQIEE309 pKa = 4.22 EE310 pKa = 4.14 NMQFNLPKK318 pKa = 10.68 LLTEE322 pKa = 4.67 DD323 pKa = 3.91 TDD325 pKa = 6.4 DD326 pKa = 4.5 EE327 pKa = 5.16 PEE329 pKa = 4.55 DD330 pKa = 4.68 NNDD333 pKa = 3.58 SMPLEE338 pKa = 4.02 EE339 pKa = 6.31 DD340 pKa = 2.77 IRR342 pKa = 11.84 EE343 pKa = 4.13 IGSMLKK349 pKa = 10.72 DD350 pKa = 3.24 GTKK353 pKa = 10.24 DD354 pKa = 2.45 IKK356 pKa = 10.37 TRR358 pKa = 11.84 MNEE361 pKa = 3.26 IDD363 pKa = 4.36 DD364 pKa = 5.01 AIKK367 pKa = 10.53 KK368 pKa = 9.49 INKK371 pKa = 8.66 KK372 pKa = 10.02 SKK374 pKa = 10.14 NRR376 pKa = 11.84 SLDD379 pKa = 3.53 LEE381 pKa = 4.54 SDD383 pKa = 3.55 GKK385 pKa = 10.72 DD386 pKa = 3.05 QGRR389 pKa = 11.84 RR390 pKa = 11.84 DD391 pKa = 3.65 PSVDD395 pKa = 3.35 LGIKK399 pKa = 9.14 KK400 pKa = 10.2 RR401 pKa = 11.84 KK402 pKa = 9.02 EE403 pKa = 3.75 GLKK406 pKa = 10.61 AAMQKK411 pKa = 8.91 TKK413 pKa = 9.98 EE414 pKa = 3.87 QLSIKK419 pKa = 8.78 VEE421 pKa = 4.07 RR422 pKa = 11.84 EE423 pKa = 3.01 IGLNDD428 pKa = 5.35 RR429 pKa = 11.84 ICQNSKK435 pKa = 9.84 MSTEE439 pKa = 3.82 KK440 pKa = 10.86 KK441 pKa = 10.16 LIYY444 pKa = 10.37 AGMEE448 pKa = 3.75 MEE450 pKa = 4.61 YY451 pKa = 10.7 GQTSTGSGGPQGSKK465 pKa = 10.68 DD466 pKa = 3.52 GTSDD470 pKa = 3.83 DD471 pKa = 3.9 VQVDD475 pKa = 3.36 EE476 pKa = 6.32 DD477 pKa = 4.26 YY478 pKa = 11.69 DD479 pKa = 3.7 EE480 pKa = 6.58 GEE482 pKa = 5.16 DD483 pKa = 3.93 YY484 pKa = 11.37 EE485 pKa = 5.59 AMPSDD490 pKa = 3.59 RR491 pKa = 11.84 FYY493 pKa = 9.19 TTLSGEE499 pKa = 4.07 QKK501 pKa = 10.94 DD502 pKa = 5.22 RR503 pKa = 11.84 FDD505 pKa = 5.02 LDD507 pKa = 3.84 ANQMSQYY514 pKa = 10.98 DD515 pKa = 3.88 LEE517 pKa = 4.53 AQVDD521 pKa = 3.96 EE522 pKa = 4.5 LTRR525 pKa = 11.84 MNLILYY531 pKa = 9.99 SRR533 pKa = 11.84 LEE535 pKa = 4.18 TTNKK539 pKa = 10.32 LLIDD543 pKa = 3.73 ILDD546 pKa = 4.21 LAKK549 pKa = 10.63 EE550 pKa = 4.05 MPKK553 pKa = 10.39 LVRR556 pKa = 11.84 KK557 pKa = 9.78 VDD559 pKa = 3.45 NLEE562 pKa = 3.94 RR563 pKa = 11.84 QMGNLNMLTSTLEE576 pKa = 3.94 GHH578 pKa = 7.15 LSSVMIMIPGKK589 pKa = 10.2 DD590 pKa = 3.28 KK591 pKa = 11.34 SEE593 pKa = 4.24 KK594 pKa = 10.09 EE595 pKa = 3.83 IPKK598 pKa = 10.6 NPDD601 pKa = 2.73 LRR603 pKa = 11.84 PILGRR608 pKa = 11.84 SNTSLTDD615 pKa = 3.84 VIDD618 pKa = 4.36 LDD620 pKa = 4.11 HH621 pKa = 7.0 YY622 pKa = 10.19 PDD624 pKa = 4.71 KK625 pKa = 10.85 GSKK628 pKa = 9.89 GIKK631 pKa = 9.44 PSGSGDD637 pKa = 3.2 RR638 pKa = 11.84 QYY640 pKa = 11.15 IGSLEE645 pKa = 4.39 SKK647 pKa = 10.5 FSINDD652 pKa = 3.3 EE653 pKa = 4.63 YY654 pKa = 11.81 NFAPYY659 pKa = 9.81 PIRR662 pKa = 11.84 DD663 pKa = 3.74 EE664 pKa = 4.51 LLLPGLRR671 pKa = 11.84 DD672 pKa = 3.73 DD673 pKa = 4.36 KK674 pKa = 11.35 TNASSFIPDD683 pKa = 3.49 DD684 pKa = 3.65 TDD686 pKa = 3.36 RR687 pKa = 11.84 SPMVLKK693 pKa = 10.59 IIIRR697 pKa = 11.84 QNIHH701 pKa = 6.49 DD702 pKa = 4.51 EE703 pKa = 4.22 EE704 pKa = 4.74 VKK706 pKa = 11.09 DD707 pKa = 3.76 EE708 pKa = 4.38 LLSILEE714 pKa = 3.83 QHH716 pKa = 5.77 NTVEE720 pKa = 4.35 EE721 pKa = 4.41 LNEE724 pKa = 3.51 IWNTVNDD731 pKa = 4.05 YY732 pKa = 11.56 LDD734 pKa = 4.0 GNII737 pKa = 4.56
Molecular weight: 83.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.794
IPC2_protein 4.698
IPC_protein 4.66
Toseland 4.507
ProMoST 4.774
Dawson 4.622
Bjellqvist 4.762
Wikipedia 4.495
Rodwell 4.507
Grimsley 4.418
Solomon 4.622
Lehninger 4.571
Nozaki 4.724
DTASelect 4.914
Thurlkill 4.507
EMBOSS 4.52
Sillero 4.787
Patrickios 4.253
IPC_peptide 4.622
IPC2_peptide 4.774
IPC2.peptide.svr19 4.746
Protein with the highest isoelectric point:
>tr|J7H4I1|J7H4I1_9MONO Isoform of J7GXK5 Nonstructural protein C OS=Cedar virus OX=1221391 GN=P PE=2 SV=1
MM1 pKa = 7.74 ASLLSILYY9 pKa = 9.99 RR10 pKa = 11.84 KK11 pKa = 8.81 IRR13 pKa = 11.84 KK14 pKa = 9.23 NYY16 pKa = 10.27 SILTEE21 pKa = 4.45 DD22 pKa = 4.81 PPSEE26 pKa = 4.05 SHH28 pKa = 6.4 PQVSGLKK35 pKa = 9.7 SGRR38 pKa = 11.84 NLFEE42 pKa = 5.72 RR43 pKa = 11.84 SLLDD47 pKa = 3.59 LNKK50 pKa = 10.31 FKK52 pKa = 11.51 GEE54 pKa = 3.98 DD55 pKa = 3.28 LRR57 pKa = 11.84 LRR59 pKa = 11.84 SQAIMEE65 pKa = 4.41 IEE67 pKa = 5.06 AILPILIRR75 pKa = 11.84 EE76 pKa = 4.63 AEE78 pKa = 4.19 SQDD81 pKa = 3.35 NSKK84 pKa = 10.9 KK85 pKa = 9.92 GIKK88 pKa = 10.08 NGGHH92 pKa = 7.5 KK93 pKa = 9.29 IQNYY97 pKa = 8.9 NWTQWLYY104 pKa = 9.98 TISSMTRR111 pKa = 11.84 EE112 pKa = 3.87 GRR114 pKa = 11.84 IPTMEE119 pKa = 4.31 NMTAALKK126 pKa = 10.86 NGIISEE132 pKa = 4.36 KK133 pKa = 9.08 EE134 pKa = 3.32 HH135 pKa = 7.04 DD136 pKa = 4.84 RR137 pKa = 11.84 ISTIISLLMNYY148 pKa = 9.33 CPAYY152 pKa = 9.9 NHH154 pKa = 7.11 LLRR157 pKa = 11.84 TMSSRR162 pKa = 11.84 MKK164 pKa = 10.11 VHH166 pKa = 5.82 QCQICMLQEE175 pKa = 3.94 INN177 pKa = 4.08
Molecular weight: 20.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.754
IPC2_protein 8.595
IPC_protein 8.58
Toseland 9.18
ProMoST 9.019
Dawson 9.472
Bjellqvist 9.297
Wikipedia 9.633
Rodwell 9.692
Grimsley 9.516
Solomon 9.575
Lehninger 9.545
Nozaki 9.428
DTASelect 9.209
Thurlkill 9.341
EMBOSS 9.633
Sillero 9.487
Patrickios 4.927
IPC_peptide 9.56
IPC2_peptide 8.024
IPC2.peptide.svr19 7.737
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
1
7
5464
177
2501
780.6
88.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.734 ± 0.695
1.574 ± 0.407
6.57 ± 0.667
5.783 ± 0.641
3.715 ± 0.445
5.179 ± 0.425
2.05 ± 0.464
8.547 ± 0.724
7.485 ± 0.318
9.023 ± 0.28
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.928 ± 0.328
7.174 ± 0.533
4.228 ± 0.188
3.459 ± 0.33
4.85 ± 0.46
8.272 ± 0.491
5.966 ± 0.222
4.758 ± 0.416
0.86 ± 0.19
3.843 ± 0.465
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here