Escherichia phage ST32

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Chaseviridae; Cleopatravirinae; Carltongylesvirus; Escherichia virus ST32

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U9DT69|A0A2U9DT69_9CAUD Uncharacterized protein OS=Escherichia phage ST32 OX=2005048 GN=ST32_0011 PE=4 SV=1
MM1 pKa = 7.18QNDD4 pKa = 4.4NITQAVEE11 pKa = 3.82QLKK14 pKa = 9.83SWRR17 pKa = 11.84RR18 pKa = 11.84DD19 pKa = 2.9EE20 pKa = 4.16TLLVRR25 pKa = 11.84FYY27 pKa = 10.62TGEE30 pKa = 4.16TNSEE34 pKa = 4.54FVDD37 pKa = 3.51AWIDD41 pKa = 3.66GLSWRR46 pKa = 11.84HH47 pKa = 5.42YY48 pKa = 10.64DD49 pKa = 3.78FDD51 pKa = 3.65MRR53 pKa = 11.84DD54 pKa = 3.79YY55 pKa = 11.33LHH57 pKa = 7.07EE58 pKa = 4.74EE59 pKa = 3.94YY60 pKa = 10.72GIEE63 pKa = 4.07YY64 pKa = 9.81PEE66 pKa = 5.34DD67 pKa = 3.26GTYY70 pKa = 11.02DD71 pKa = 3.49VIEE74 pKa = 4.57SNEE77 pKa = 3.72EE78 pKa = 3.88DD79 pKa = 3.52VTEE82 pKa = 4.14VCFVDD87 pKa = 3.9GLFSIRR93 pKa = 11.84KK94 pKa = 7.32YY95 pKa = 11.46DD96 pKa = 3.41KK97 pKa = 10.92FNEE100 pKa = 4.17LLVDD104 pKa = 3.63TDD106 pKa = 4.55LDD108 pKa = 4.01VILAAFRR115 pKa = 11.84SGDD118 pKa = 3.06IDD120 pKa = 3.72IFTVDD125 pKa = 4.29DD126 pKa = 4.7LNDD129 pKa = 3.72KK130 pKa = 9.67VICRR134 pKa = 11.84CNSIADD140 pKa = 3.63YY141 pKa = 9.63FQQYY145 pKa = 11.03DD146 pKa = 4.1GIEE149 pKa = 4.41VPDD152 pKa = 4.04HH153 pKa = 5.92LRR155 pKa = 11.84PYY157 pKa = 10.2IDD159 pKa = 3.26WDD161 pKa = 3.56AVARR165 pKa = 11.84DD166 pKa = 3.78YY167 pKa = 11.83GSDD170 pKa = 3.43YY171 pKa = 10.74TFSEE175 pKa = 5.41GILFSNAA182 pKa = 2.85

Molecular weight: 21.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U9DT30|A0A2U9DT30_9CAUD Uncharacterized protein OS=Escherichia phage ST32 OX=2005048 GN=ST32_0020 PE=4 SV=1
MM1 pKa = 7.7RR2 pKa = 11.84VNTHH6 pKa = 6.7LYY8 pKa = 8.03SHH10 pKa = 7.3KK11 pKa = 10.41SHH13 pKa = 7.34SPMNIFAGWTLWFVGEE29 pKa = 4.61EE30 pKa = 3.97CSPWHH35 pKa = 5.87NVEE38 pKa = 4.85CKK40 pKa = 10.2FFPVKK45 pKa = 8.86PTKK48 pKa = 10.19KK49 pKa = 9.92QIRR52 pKa = 11.84KK53 pKa = 8.83LRR55 pKa = 11.84RR56 pKa = 11.84KK57 pKa = 9.78FINDD61 pKa = 3.5CKK63 pKa = 10.96SCC65 pKa = 3.62

Molecular weight: 7.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

15743

45

1241

199.3

22.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.162 ± 0.375

1.201 ± 0.151

5.876 ± 0.275

6.625 ± 0.29

4.034 ± 0.216

7.311 ± 0.322

1.982 ± 0.19

6.149 ± 0.224

6.161 ± 0.33

7.743 ± 0.298

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.846 ± 0.209

4.993 ± 0.191

3.792 ± 0.182

3.843 ± 0.205

4.681 ± 0.218

6.682 ± 0.356

6.034 ± 0.325

6.911 ± 0.326

1.372 ± 0.112

3.602 ± 0.159
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski