Amino acid dipepetide frequency for Brachionus plicatilis (Marine rotifer) (Brachionus muelleri)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	2.538AlaAla: 2.538 ± 0.025	0.88AlaCys: 0.88 ± 0.009	2.115AlaAsp: 2.115 ± 0.015	2.563AlaGlu: 2.563 ± 0.017	2.295AlaPhe: 2.295 ± 0.017	1.808AlaGly: 1.808 ± 0.018	0.981AlaHis: 0.981 ± 0.009	3.051AlaIle: 3.051 ± 0.019	3.749AlaLys: 3.749 ± 0.02	4.208AlaLeu: 4.208 ± 0.023	0.89AlaMet: 0.89 ± 0.009	2.825AlaAsn: 2.825 ± 0.018	1.462AlaPro: 1.462 ± 0.015	1.772AlaGln: 1.772 ± 0.016	1.895AlaArg: 1.895 ± 0.014	3.671AlaSer: 3.671 ± 0.02	2.109AlaThr: 2.109 ± 0.015	2.348AlaVal: 2.348 ± 0.015	0.379AlaTrp: 0.379 ± 0.006	1.411AlaTyr: 1.411 ± 0.013	0.002AlaXaa: 0.002 ± 0.0
Cys	0.813CysAla: 0.813 ± 0.009	0.515CysCys: 0.515 ± 0.009	1.084CysAsp: 1.084 ± 0.013	1.179CysGlu: 1.179 ± 0.014	1.261CysPhe: 1.261 ± 0.013	0.968CysGly: 0.968 ± 0.012	0.47CysHis: 0.47 ± 0.006	1.369CysIle: 1.369 ± 0.014	1.417CysLys: 1.417 ± 0.013	2.373CysLeu: 2.373 ± 0.019	0.408CysMet: 0.408 ± 0.007	1.069CysAsn: 1.069 ± 0.014	0.814CysPro: 0.814 ± 0.012	0.904CysGln: 0.904 ± 0.014	0.898CysArg: 0.898 ± 0.009	1.94CysSer: 1.94 ± 0.016	0.916CysThr: 0.916 ± 0.01	1.151CysVal: 1.151 ± 0.013	0.243CysTrp: 0.243 ± 0.005	0.721CysTyr: 0.721 ± 0.008	0.001CysXaa: 0.001 ± 0.0
Asp	2.004AspAla: 2.004 ± 0.017	0.987AspCys: 0.987 ± 0.01	3.11AspAsp: 3.11 ± 0.027	3.898AspGlu: 3.898 ± 0.025	3.174AspPhe: 3.174 ± 0.019	1.978AspGly: 1.978 ± 0.015	1.123AspHis: 1.123 ± 0.011	3.283AspIle: 3.283 ± 0.018	3.84AspLys: 3.84 ± 0.023	5.794AspLeu: 5.794 ± 0.028	1.052AspMet: 1.052 ± 0.01	2.765AspAsn: 2.765 ± 0.017	1.714AspPro: 1.714 ± 0.013	2.434AspGln: 2.434 ± 0.016	2.004AspArg: 2.004 ± 0.016	4.235AspSer: 4.235 ± 0.022	2.182AspThr: 2.182 ± 0.015	2.239AspVal: 2.239 ± 0.015	0.544AspTrp: 0.544 ± 0.008	1.97AspTyr: 1.97 ± 0.015	0.002AspXaa: 0.002 ± 0.0
Glu	2.68GluAla: 2.68 ± 0.019	1.187GluCys: 1.187 ± 0.013	2.839GluAsp: 2.839 ± 0.02	4.455GluGlu: 4.455 ± 0.032	3.105GluPhe: 3.105 ± 0.018	1.498GluGly: 1.498 ± 0.013	1.208GluHis: 1.208 ± 0.013	5.138GluIle: 5.138 ± 0.025	6.276GluLys: 6.276 ± 0.029	5.945GluLeu: 5.945 ± 0.029	1.416GluMet: 1.416 ± 0.01	5.136GluAsn: 5.136 ± 0.025	1.923GluPro: 1.923 ± 0.015	2.602GluGln: 2.602 ± 0.023	2.654GluArg: 2.654 ± 0.02	4.895GluSer: 4.895 ± 0.026	3.109GluThr: 3.109 ± 0.016	2.4GluVal: 2.4 ± 0.017	0.602GluTrp: 0.602 ± 0.008	2.112GluTyr: 2.112 ± 0.016	0.003GluXaa: 0.003 ± 0.0
Phe	2.156PheAla: 2.156 ± 0.015	1.393PheCys: 1.393 ± 0.013	3.446PheAsp: 3.446 ± 0.018	3.683PheGlu: 3.683 ± 0.021	3.962PhePhe: 3.962 ± 0.026	2.755PheGly: 2.755 ± 0.019	1.135PheHis: 1.135 ± 0.012	4.087PheIle: 4.087 ± 0.025	4.777PheLys: 4.777 ± 0.021	6.083PheLeu: 6.083 ± 0.03	1.277PheMet: 1.277 ± 0.01	3.876PheAsn: 3.876 ± 0.022	1.541PhePro: 1.541 ± 0.011	1.973PheGln: 1.973 ± 0.014	2.293PheArg: 2.293 ± 0.015	4.998PheSer: 4.998 ± 0.025	2.563PheThr: 2.563 ± 0.017	3.201PheVal: 3.201 ± 0.018	0.62PheTrp: 0.62 ± 0.008	2.523PheTyr: 2.523 ± 0.015	0.002PheXaa: 0.002 ± 0.0
Gly	1.817GlyAla: 1.817 ± 0.015	0.807GlyCys: 0.807 ± 0.01	1.848GlyAsp: 1.848 ± 0.016	2.004GlyGlu: 2.004 ± 0.017	2.251GlyPhe: 2.251 ± 0.018	1.963GlyGly: 1.963 ± 0.022	1.078GlyHis: 1.078 ± 0.011	2.768GlyIle: 2.768 ± 0.017	2.836GlyLys: 2.836 ± 0.018	4.019GlyLeu: 4.019 ± 0.026	0.761GlyMet: 0.761 ± 0.009	2.116GlyAsn: 2.116 ± 0.014	1.312GlyPro: 1.312 ± 0.015	1.708GlyGln: 1.708 ± 0.015	1.9GlyArg: 1.9 ± 0.017	3.283GlySer: 3.283 ± 0.02	1.979GlyThr: 1.979 ± 0.015	2.01GlyVal: 2.01 ± 0.015	0.4GlyTrp: 0.4 ± 0.007	1.501GlyTyr: 1.501 ± 0.015	0.002GlyXaa: 0.002 ± 0.0
His	0.815HisAla: 0.815 ± 0.01	0.435HisCys: 0.435 ± 0.006	0.93HisAsp: 0.93 ± 0.01	1.151HisGlu: 1.151 ± 0.011	1.44HisPhe: 1.44 ± 0.012	0.875HisGly: 0.875 ± 0.009	0.673HisHis: 0.673 ± 0.011	1.363HisIle: 1.363 ± 0.012	1.532HisLys: 1.532 ± 0.013	2.506HisLeu: 2.506 ± 0.016	0.46HisMet: 0.46 ± 0.007	1.197HisAsn: 1.197 ± 0.01	0.751HisPro: 0.751 ± 0.009	1.012HisGln: 1.012 ± 0.011	0.921HisArg: 0.921 ± 0.01	1.786HisSer: 1.786 ± 0.013	0.916HisThr: 0.916 ± 0.011	1.139HisVal: 1.139 ± 0.012	0.211HisTrp: 0.211 ± 0.005	0.788HisTyr: 0.788 ± 0.01	0.001HisXaa: 0.001 ± 0.0
Ile	2.989IleAla: 2.989 ± 0.019	1.598IleCys: 1.598 ± 0.013	4.396IleAsp: 4.396 ± 0.023	5.143IleGlu: 5.143 ± 0.027	4.836IlePhe: 4.836 ± 0.026	3.028IleGly: 3.028 ± 0.018	1.425IleHis: 1.425 ± 0.01	5.49IleIle: 5.49 ± 0.03	7.027IleLys: 7.027 ± 0.031	7.713IleLeu: 7.713 ± 0.029	1.64IleMet: 1.64 ± 0.013	5.588IleAsn: 5.588 ± 0.022	2.382IlePro: 2.382 ± 0.015	2.984IleGln: 2.984 ± 0.019	3.242IleArg: 3.242 ± 0.017	6.073IleSer: 6.073 ± 0.024	3.214IleThr: 3.214 ± 0.018	3.808IleVal: 3.808 ± 0.023	0.779IleTrp: 0.779 ± 0.011	2.841IleTyr: 2.841 ± 0.017	0.003IleXaa: 0.003 ± 0.001
Lys	3.25LysAla: 3.25 ± 0.021	1.785LysCys: 1.785 ± 0.014	3.48LysAsp: 3.48 ± 0.022	5.087LysGlu: 5.087 ± 0.029	4.83LysPhe: 4.83 ± 0.023	2.187LysGly: 2.187 ± 0.016	1.587LysHis: 1.587 ± 0.012	8.372LysIle: 8.372 ± 0.032	9.586LysLys: 9.586 ± 0.042	8.592LysLeu: 8.592 ± 0.03	2.104LysMet: 2.104 ± 0.013	7.477LysAsn: 7.477 ± 0.031	2.671LysPro: 2.671 ± 0.02	3.307LysGln: 3.307 ± 0.021	4.0LysArg: 4.0 ± 0.02	7.275LysSer: 7.275 ± 0.03	4.511LysThr: 4.511 ± 0.025	3.416LysVal: 3.416 ± 0.017	0.807LysTrp: 0.807 ± 0.009	3.247LysTyr: 3.247 ± 0.018	0.003LysXaa: 0.003 ± 0.0
Leu	4.881LeuAla: 4.881 ± 0.022	1.872LeuCys: 1.872 ± 0.015	5.356LeuAsp: 5.356 ± 0.027	5.986LeuGlu: 5.986 ± 0.029	5.761LeuPhe: 5.761 ± 0.027	3.815LeuGly: 3.815 ± 0.021	1.876LeuHis: 1.876 ± 0.014	8.365LeuIle: 8.365 ± 0.033	9.735LeuLys: 9.735 ± 0.037	8.838LeuLeu: 8.838 ± 0.04	2.234LeuMet: 2.234 ± 0.014	8.585LeuAsn: 8.585 ± 0.034	3.232LeuPro: 3.232 ± 0.02	3.217LeuGln: 3.217 ± 0.021	4.167LeuArg: 4.167 ± 0.022	8.406LeuSer: 8.406 ± 0.03	5.089LeuThr: 5.089 ± 0.022	5.815LeuVal: 5.815 ± 0.03	0.812LeuTrp: 0.812 ± 0.008	3.088LeuTyr: 3.088 ± 0.017	0.005LeuXaa: 0.005 ± 0.001
Met	1.187MetAla: 1.187 ± 0.01	0.431MetCys: 0.431 ± 0.006	1.13MetAsp: 1.13 ± 0.011	1.28MetGlu: 1.28 ± 0.012	1.091MetPhe: 1.091 ± 0.01	0.797MetGly: 0.797 ± 0.01	0.483MetHis: 0.483 ± 0.007	1.733MetIle: 1.733 ± 0.012	1.742MetLys: 1.742 ± 0.013	1.958MetLeu: 1.958 ± 0.014	0.487MetMet: 0.487 ± 0.007	1.654MetAsn: 1.654 ± 0.013	0.812MetPro: 0.812 ± 0.01	0.746MetGln: 0.746 ± 0.009	0.92MetArg: 0.92 ± 0.01	2.021MetSer: 2.021 ± 0.015	1.185MetThr: 1.185 ± 0.011	1.222MetVal: 1.222 ± 0.01	0.158MetTrp: 0.158 ± 0.003	0.657MetTyr: 0.657 ± 0.007	0.001MetXaa: 0.001 ± 0.0
Asn	2.535AsnAla: 2.535 ± 0.016	1.53AsnCys: 1.53 ± 0.014	3.536AsnAsp: 3.536 ± 0.017	4.864AsnGlu: 4.864 ± 0.027	4.858AsnPhe: 4.858 ± 0.027	3.126AsnGly: 3.126 ± 0.021	1.402AsnHis: 1.402 ± 0.012	4.836AsnIle: 4.836 ± 0.025	5.8AsnLys: 5.8 ± 0.026	8.112AsnLeu: 8.112 ± 0.032	1.579AsnMet: 1.579 ± 0.012	4.583AsnAsn: 4.583 ± 0.028	2.255AsnPro: 2.255 ± 0.02	3.531AsnGln: 3.531 ± 0.023	3.059AsnArg: 3.059 ± 0.019	6.567AsnSer: 6.567 ± 0.033	2.996AsnThr: 2.996 ± 0.015	3.163AsnVal: 3.163 ± 0.018	0.684AsnTrp: 0.684 ± 0.008	2.814AsnTyr: 2.814 ± 0.017	0.002AsnXaa: 0.002 ± 0.0
Pro	1.557ProAla: 1.557 ± 0.014	0.661ProCys: 0.661 ± 0.014	1.653ProAsp: 1.653 ± 0.013	1.91ProGlu: 1.91 ± 0.013	1.818ProPhe: 1.818 ± 0.014	1.329ProGly: 1.329 ± 0.02	0.661ProHis: 0.661 ± 0.008	2.525ProIle: 2.525 ± 0.017	2.953ProLys: 2.953 ± 0.019	3.01ProLeu: 3.01 ± 0.021	0.649ProMet: 0.649 ± 0.009	2.558ProAsn: 2.558 ± 0.018	1.504ProPro: 1.504 ± 0.022	1.245ProGln: 1.245 ± 0.013	1.415ProArg: 1.415 ± 0.013	3.038ProSer: 3.038 ± 0.019	1.802ProThr: 1.802 ± 0.013	2.065ProVal: 2.065 ± 0.019	0.268ProTrp: 0.268 ± 0.005	1.104ProTyr: 1.104 ± 0.013	0.003ProXaa: 0.003 ± 0.001
Gln	1.825GlnAla: 1.825 ± 0.015	0.672GlnCys: 0.672 ± 0.01	1.649GlnAsp: 1.649 ± 0.011	2.213GlnGlu: 2.213 ± 0.016	2.037GlnPhe: 2.037 ± 0.014	1.077GlnGly: 1.077 ± 0.012	0.866GlnHis: 0.866 ± 0.009	3.693GlnIle: 3.693 ± 0.019	3.665GlnLys: 3.665 ± 0.02	3.992GlnLeu: 3.992 ± 0.021	1.045GlnMet: 1.045 ± 0.011	3.567GlnAsn: 3.567 ± 0.018	1.416GlnPro: 1.416 ± 0.015	1.997GlnGln: 1.997 ± 0.029	1.665GlnArg: 1.665 ± 0.013	3.344GlnSer: 3.344 ± 0.02	2.321GlnThr: 2.321 ± 0.016	2.006GlnVal: 2.006 ± 0.014	0.309GlnTrp: 0.309 ± 0.005	1.238GlnTyr: 1.238 ± 0.012	0.002GlnXaa: 0.002 ± 0.0
Arg	1.896ArgAla: 1.896 ± 0.015	0.815ArgCys: 0.815 ± 0.011	1.885ArgAsp: 1.885 ± 0.015	2.362ArgGlu: 2.362 ± 0.017	2.314ArgPhe: 2.314 ± 0.017	1.55ArgGly: 1.55 ± 0.014	0.992ArgHis: 0.992 ± 0.011	3.398ArgIle: 3.398 ± 0.019	3.859ArgLys: 3.859 ± 0.022	4.648ArgLeu: 4.648 ± 0.019	0.905ArgMet: 0.905 ± 0.01	2.817ArgAsn: 2.817 ± 0.018	1.858ArgPro: 1.858 ± 0.017	1.769ArgGln: 1.769 ± 0.014	2.304ArgArg: 2.304 ± 0.022	3.451ArgSer: 3.451 ± 0.022	2.179ArgThr: 2.179 ± 0.013	2.302ArgVal: 2.302 ± 0.017	0.376ArgTrp: 0.376 ± 0.006	1.471ArgTyr: 1.471 ± 0.012	0.002ArgXaa: 0.002 ± 0.0
Ser	3.744SerAla: 3.744 ± 0.023	1.693SerCys: 1.693 ± 0.013	4.428SerAsp: 4.428 ± 0.024	4.851SerGlu: 4.851 ± 0.024	4.846SerPhe: 4.846 ± 0.029	3.457SerGly: 3.457 ± 0.018	1.761SerHis: 1.761 ± 0.014	6.621SerIle: 6.621 ± 0.027	7.114SerLys: 7.114 ± 0.029	8.574SerLeu: 8.574 ± 0.029	1.753SerMet: 1.753 ± 0.012	6.331SerAsn: 6.331 ± 0.028	2.754SerPro: 2.754 ± 0.019	3.427SerGln: 3.427 ± 0.022	3.525SerArg: 3.525 ± 0.022	9.381SerSer: 9.381 ± 0.059	4.524SerThr: 4.524 ± 0.027	4.352SerVal: 4.352 ± 0.022	0.731SerTrp: 0.731 ± 0.008	2.742SerTyr: 2.742 ± 0.018	0.002SerXaa: 0.002 ± 0.0
Thr	2.069ThrAla: 2.069 ± 0.014	1.069ThrCys: 1.069 ± 0.016	2.361ThrAsp: 2.361 ± 0.015	2.819ThrGlu: 2.819 ± 0.019	2.65ThrPhe: 2.65 ± 0.018	2.129ThrGly: 2.129 ± 0.019	0.94ThrHis: 0.94 ± 0.01	3.885ThrIle: 3.885 ± 0.018	4.242ThrLys: 4.242 ± 0.021	4.586ThrLeu: 4.586 ± 0.02	1.011ThrMet: 1.011 ± 0.01	3.644ThrAsn: 3.644 ± 0.019	1.8ThrPro: 1.8 ± 0.014	1.868ThrGln: 1.868 ± 0.013	2.09ThrArg: 2.09 ± 0.015	4.349ThrSer: 4.349 ± 0.027	2.845ThrThr: 2.845 ± 0.026	2.496ThrVal: 2.496 ± 0.018	0.481ThrTrp: 0.481 ± 0.007	1.541ThrTyr: 1.541 ± 0.012	0.002ThrXaa: 0.002 ± 0.0
Val	2.37ValAla: 2.37 ± 0.016	1.153ValCys: 1.153 ± 0.012	2.806ValAsp: 2.806 ± 0.017	3.201ValGlu: 3.201 ± 0.017	2.738ValPhe: 2.738 ± 0.018	2.133ValGly: 2.133 ± 0.02	1.186ValHis: 1.186 ± 0.009	3.245ValIle: 3.245 ± 0.02	4.045ValLys: 4.045 ± 0.023	5.023ValLeu: 5.023 ± 0.021	1.002ValMet: 1.002 ± 0.01	3.27ValAsn: 3.27 ± 0.02	1.986ValPro: 1.986 ± 0.02	2.173ValGln: 2.173 ± 0.015	2.215ValArg: 2.215 ± 0.015	3.98ValSer: 3.98 ± 0.022	2.355ValThr: 2.355 ± 0.016	3.079ValVal: 3.079 ± 0.024	0.508ValTrp: 0.508 ± 0.007	1.897ValTyr: 1.897 ± 0.016	0.003ValXaa: 0.003 ± 0.0
Trp	0.373TrpAla: 0.373 ± 0.006	0.211TrpCys: 0.211 ± 0.004	0.433TrpAsp: 0.433 ± 0.008	0.369TrpGlu: 0.369 ± 0.006	0.556TrpPhe: 0.556 ± 0.008	0.309TrpGly: 0.309 ± 0.007	0.232TrpHis: 0.232 ± 0.005	0.75TrpIle: 0.75 ± 0.008	0.751TrpLys: 0.751 ± 0.008	1.008TrpLeu: 1.008 ± 0.009	0.219TrpMet: 0.219 ± 0.004	0.735TrpAsn: 0.735 ± 0.01	0.36TrpPro: 0.36 ± 0.007	0.346TrpGln: 0.346 ± 0.006	0.47TrpArg: 0.47 ± 0.006	0.874TrpSer: 0.874 ± 0.01	0.543TrpThr: 0.543 ± 0.009	0.405TrpVal: 0.405 ± 0.006	0.114TrpTrp: 0.114 ± 0.004	0.36TrpTyr: 0.36 ± 0.006	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.454TyrAla: 1.454 ± 0.012	0.82TyrCys: 0.82 ± 0.011	1.845TyrAsp: 1.845 ± 0.017	2.159TyrGlu: 2.159 ± 0.016	2.518TyrPhe: 2.518 ± 0.016	1.578TyrGly: 1.578 ± 0.016	0.822TyrHis: 0.822 ± 0.01	2.221TyrIle: 2.221 ± 0.014	2.625TyrLys: 2.625 ± 0.016	4.152TyrLeu: 4.152 ± 0.023	0.802TyrMet: 0.802 ± 0.01	2.037TyrAsn: 2.037 ± 0.016	1.181TyrPro: 1.181 ± 0.013	1.582TyrGln: 1.582 ± 0.012	1.515TyrArg: 1.515 ± 0.013	3.038TyrSer: 3.038 ± 0.017	1.537TyrThr: 1.537 ± 0.013	1.747TyrVal: 1.747 ± 0.012	0.394TyrTrp: 0.394 ± 0.007	1.486TyrTyr: 1.486 ± 0.016	0.001TyrXaa: 0.001 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.0	0.001XaaCys: 0.001 ± 0.0	0.002XaaAsp: 0.002 ± 0.0	0.002XaaGlu: 0.002 ± 0.0	0.002XaaPhe: 0.002 ± 0.0	0.002XaaGly: 0.002 ± 0.0	0.001XaaHis: 0.001 ± 0.0	0.002XaaIle: 0.002 ± 0.0	0.003XaaLys: 0.003 ± 0.001	0.003XaaLeu: 0.003 ± 0.0	0.002XaaMet: 0.002 ± 0.0	0.003XaaAsn: 0.003 ± 0.0	0.001XaaPro: 0.001 ± 0.0	0.002XaaGln: 0.002 ± 0.0	0.002XaaArg: 0.002 ± 0.0	0.004XaaSer: 0.004 ± 0.001	0.002XaaThr: 0.002 ± 0.0	0.003XaaVal: 0.003 ± 0.001	0.001XaaTrp: 0.001 ± 0.0	0.001XaaTyr: 0.001 ± 0.0	0.662XaaXaa: 0.662 ± 0.045

Statistics based on 52387 proteins (10963705 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---