Naasia lichenicola
Average proteome isoelectric point is 5.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3498 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4S4FIG4|A0A4S4FIG4_9MICO Uncharacterized protein OS=Naasia lichenicola OX=2565933 GN=E6C64_13235 PE=4 SV=1
MM1 pKa = 7.77 SGFEE5 pKa = 3.89 KK6 pKa = 10.76 AKK8 pKa = 10.34 ASRR11 pKa = 11.84 SRR13 pKa = 11.84 TLKK16 pKa = 10.56 NVLGGVAVIGVSVLFLSACSPTASTGSSEE45 pKa = 4.27 TAAPPTDD52 pKa = 4.48 DD53 pKa = 3.87 LTLKK57 pKa = 10.43 IGTALPQTGNLAYY70 pKa = 10.61 LGPPEE75 pKa = 4.13 EE76 pKa = 5.76 AGVQYY81 pKa = 10.73 AISEE85 pKa = 4.37 INAATADD92 pKa = 3.39 TGLTVDD98 pKa = 4.65 LTLGDD103 pKa = 5.43 SGDD106 pKa = 4.03 TDD108 pKa = 3.11 NKK110 pKa = 10.74 AYY112 pKa = 8.31 EE113 pKa = 4.24 TEE115 pKa = 3.95 IPRR118 pKa = 11.84 LLGDD122 pKa = 3.91 GVSAIIGAASSGTSLKK138 pKa = 10.9 FIDD141 pKa = 4.45 DD142 pKa = 3.73 VVGAGVIQFSPANTSDD158 pKa = 3.9 AFTTYY163 pKa = 10.71 DD164 pKa = 4.0 DD165 pKa = 3.44 KK166 pKa = 11.81 GLYY169 pKa = 9.82 FRR171 pKa = 11.84 TAPSDD176 pKa = 3.66 VLQGEE181 pKa = 4.54 VLGNLIAEE189 pKa = 5.36 DD190 pKa = 4.32 GNQTLGLIVLNDD202 pKa = 3.48 SYY204 pKa = 11.65 GTGLAKK210 pKa = 10.55 YY211 pKa = 8.76 VTDD214 pKa = 3.48 SFEE217 pKa = 4.05 AAGGEE222 pKa = 4.58 VVAAPTYY229 pKa = 8.88 NTGDD233 pKa = 3.41 TSFDD237 pKa = 3.52 SQIQEE242 pKa = 4.12 VLAADD247 pKa = 4.23 PDD249 pKa = 4.68 AIALITFEE257 pKa = 4.29 EE258 pKa = 5.0 VKK260 pKa = 10.26 TILPGLLASFPSDD273 pKa = 2.34 KK274 pKa = 11.01 LYY276 pKa = 10.88 FVDD279 pKa = 5.61 GNLKK283 pKa = 10.48 NFGDD287 pKa = 4.21 EE288 pKa = 4.34 LPAGSLTGAKK298 pKa = 8.09 GTLPGLSIDD307 pKa = 3.98 SLTDD311 pKa = 3.22 FTGALDD317 pKa = 4.53 DD318 pKa = 4.25 FASSEE323 pKa = 4.1 GLPALKK329 pKa = 10.16 DD330 pKa = 3.35 YY331 pKa = 11.24 SYY333 pKa = 11.43 AAEE336 pKa = 4.55 SYY338 pKa = 10.77 DD339 pKa = 3.52 ATVLLALGALAAGSTDD355 pKa = 3.19 GTDD358 pKa = 2.92 IAGKK362 pKa = 9.03 LQEE365 pKa = 4.15 VSGGSGDD372 pKa = 3.98 GEE374 pKa = 4.44 KK375 pKa = 9.99 CTTYY379 pKa = 11.08 ADD381 pKa = 3.61 CAAIIVGGGTADD393 pKa = 3.39 YY394 pKa = 10.88 DD395 pKa = 4.59 GISGPISFDD404 pKa = 4.37 DD405 pKa = 4.26 VGDD408 pKa = 4.1 PQDD411 pKa = 3.49 ASIGIYY417 pKa = 10.52 VYY419 pKa = 11.18 GEE421 pKa = 4.17 DD422 pKa = 3.76 NNYY425 pKa = 8.04 TAYY428 pKa = 10.26 EE429 pKa = 4.27 GG430 pKa = 3.63
Molecular weight: 43.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.691
IPC2_protein 3.63
IPC_protein 3.668
Toseland 3.439
ProMoST 3.846
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.617
Rodwell 3.49
Grimsley 3.35
Solomon 3.668
Lehninger 3.617
Nozaki 3.783
DTASelect 4.05
Thurlkill 3.49
EMBOSS 3.63
Sillero 3.795
Patrickios 1.265
IPC_peptide 3.656
IPC2_peptide 3.77
IPC2.peptide.svr19 3.728
Protein with the highest isoelectric point:
>tr|A0A4S4FP30|A0A4S4FP30_9MICO NAD-dependent epimerase/dehydratase family protein OS=Naasia lichenicola OX=2565933 GN=E6C64_04745 PE=3 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.87 KK16 pKa = 9.7 HH17 pKa = 5.84 RR18 pKa = 11.84 KK19 pKa = 8.56 LLRR22 pKa = 11.84 KK23 pKa = 7.78 TRR25 pKa = 11.84 HH26 pKa = 3.65 QRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.78 KK32 pKa = 9.85
Molecular weight: 4.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3498
0
3498
1124881
27
2031
321.6
34.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.084 ± 0.052
0.451 ± 0.009
6.246 ± 0.038
5.461 ± 0.041
3.177 ± 0.025
8.996 ± 0.036
1.889 ± 0.023
4.973 ± 0.031
1.974 ± 0.027
10.205 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.746 ± 0.015
1.992 ± 0.023
5.376 ± 0.034
2.81 ± 0.021
7.108 ± 0.047
6.501 ± 0.035
6.024 ± 0.041
8.584 ± 0.036
1.412 ± 0.018
1.991 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here