Amino acid dipepetide frequency for Streptococcus virus Sfi21

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.054AlaAla: 3.054 ± 0.81
0.37AlaCys: 0.37 ± 0.212
5.552AlaAsp: 5.552 ± 1.145
3.701AlaGlu: 3.701 ± 0.421
2.313AlaPhe: 2.313 ± 0.625
3.331AlaGly: 3.331 ± 0.593
0.74AlaHis: 0.74 ± 0.264
5.274AlaIle: 5.274 ± 1.031
5.552AlaLys: 5.552 ± 1.155
5.182AlaLeu: 5.182 ± 0.634
2.128AlaMet: 2.128 ± 0.56
4.534AlaAsn: 4.534 ± 0.796
1.388AlaPro: 1.388 ± 0.403
3.146AlaGln: 3.146 ± 0.644
2.221AlaArg: 2.221 ± 0.381
5.274AlaSer: 5.274 ± 0.753
4.164AlaThr: 4.164 ± 0.826
3.239AlaVal: 3.239 ± 0.576
1.11AlaTrp: 1.11 ± 0.301
3.146AlaTyr: 3.146 ± 0.567
0.0AlaXaa: 0.0 ± 0.0
Cys
0.093CysAla: 0.093 ± 0.085
0.0CysCys: 0.0 ± 0.0
0.555CysAsp: 0.555 ± 0.241
0.555CysGlu: 0.555 ± 0.262
0.278CysPhe: 0.278 ± 0.205
0.278CysGly: 0.278 ± 0.151
0.093CysHis: 0.093 ± 0.094
0.185CysIle: 0.185 ± 0.126
0.185CysLys: 0.185 ± 0.132
0.555CysLeu: 0.555 ± 0.228
0.278CysMet: 0.278 ± 0.201
0.37CysAsn: 0.37 ± 0.169
0.278CysPro: 0.278 ± 0.18
0.185CysGln: 0.185 ± 0.11
0.0CysArg: 0.0 ± 0.0
0.37CysSer: 0.37 ± 0.21
0.185CysThr: 0.185 ± 0.132
0.37CysVal: 0.37 ± 0.155
0.278CysTrp: 0.278 ± 0.179
0.278CysTyr: 0.278 ± 0.17
0.0CysXaa: 0.0 ± 0.0
Asp
3.331AspAla: 3.331 ± 0.587
0.463AspCys: 0.463 ± 0.222
4.534AspAsp: 4.534 ± 1.024
4.257AspGlu: 4.257 ± 0.697
3.979AspPhe: 3.979 ± 0.547
7.773AspGly: 7.773 ± 1.485
0.463AspHis: 0.463 ± 0.192
6.015AspIle: 6.015 ± 0.678
5.737AspLys: 5.737 ± 0.678
4.257AspLeu: 4.257 ± 0.898
1.851AspMet: 1.851 ± 0.407
4.257AspAsn: 4.257 ± 0.629
2.313AspPro: 2.313 ± 0.487
1.295AspGln: 1.295 ± 0.281
2.221AspArg: 2.221 ± 0.57
4.164AspSer: 4.164 ± 0.762
4.257AspThr: 4.257 ± 0.601
2.869AspVal: 2.869 ± 0.615
1.203AspTrp: 1.203 ± 0.284
3.146AspTyr: 3.146 ± 0.602
0.0AspXaa: 0.0 ± 0.0
Glu
3.886GluAla: 3.886 ± 0.694
0.37GluCys: 0.37 ± 0.198
2.683GluAsp: 2.683 ± 0.604
3.886GluGlu: 3.886 ± 0.965
2.591GluPhe: 2.591 ± 0.459
3.424GluGly: 3.424 ± 0.482
1.295GluHis: 1.295 ± 0.327
5.552GluIle: 5.552 ± 0.821
4.071GluLys: 4.071 ± 0.822
5.459GluLeu: 5.459 ± 0.826
1.943GluMet: 1.943 ± 0.376
4.071GluAsn: 4.071 ± 0.662
2.036GluPro: 2.036 ± 0.45
2.406GluGln: 2.406 ± 0.358
2.591GluArg: 2.591 ± 0.467
2.221GluSer: 2.221 ± 0.408
3.886GluThr: 3.886 ± 0.724
5.089GluVal: 5.089 ± 0.633
0.833GluTrp: 0.833 ± 0.246
3.331GluTyr: 3.331 ± 0.666
0.0GluXaa: 0.0 ± 0.0
Phe
2.776PheAla: 2.776 ± 0.48
0.185PheCys: 0.185 ± 0.13
3.516PheAsp: 3.516 ± 0.651
1.758PheGlu: 1.758 ± 0.35
1.851PhePhe: 1.851 ± 0.268
3.609PheGly: 3.609 ± 0.701
0.555PheHis: 0.555 ± 0.184
2.776PheIle: 2.776 ± 0.651
4.164PheLys: 4.164 ± 0.511
3.331PheLeu: 3.331 ± 0.551
0.648PheMet: 0.648 ± 0.254
3.424PheAsn: 3.424 ± 0.635
0.833PhePro: 0.833 ± 0.294
1.203PheGln: 1.203 ± 0.337
1.666PheArg: 1.666 ± 0.377
3.239PheSer: 3.239 ± 0.746
2.221PheThr: 2.221 ± 0.627
3.054PheVal: 3.054 ± 0.455
0.555PheTrp: 0.555 ± 0.249
1.666PheTyr: 1.666 ± 0.326
0.0PheXaa: 0.0 ± 0.0
Gly
3.979GlyAla: 3.979 ± 0.941
0.093GlyCys: 0.093 ± 0.103
4.534GlyAsp: 4.534 ± 0.868
2.869GlyGlu: 2.869 ± 0.568
2.406GlyPhe: 2.406 ± 0.325
4.627GlyGly: 4.627 ± 0.804
1.11GlyHis: 1.11 ± 0.283
5.83GlyIle: 5.83 ± 0.671
6.015GlyLys: 6.015 ± 0.802
6.2GlyLeu: 6.2 ± 0.678
1.851GlyMet: 1.851 ± 0.363
4.997GlyAsn: 4.997 ± 0.939
1.11GlyPro: 1.11 ± 0.574
2.776GlyGln: 2.776 ± 0.541
3.794GlyArg: 3.794 ± 0.645
4.904GlySer: 4.904 ± 0.716
4.442GlyThr: 4.442 ± 0.728
2.869GlyVal: 2.869 ± 0.567
1.388GlyTrp: 1.388 ± 0.391
3.054GlyTyr: 3.054 ± 0.524
0.0GlyXaa: 0.0 ± 0.0
His
0.37HisAla: 0.37 ± 0.169
0.0HisCys: 0.0 ± 0.0
0.833HisAsp: 0.833 ± 0.211
1.11HisGlu: 1.11 ± 0.35
0.833HisPhe: 0.833 ± 0.208
0.925HisGly: 0.925 ± 0.251
0.37HisHis: 0.37 ± 0.142
1.11HisIle: 1.11 ± 0.307
0.833HisLys: 0.833 ± 0.336
1.388HisLeu: 1.388 ± 0.429
0.648HisMet: 0.648 ± 0.267
0.555HisAsn: 0.555 ± 0.31
0.648HisPro: 0.648 ± 0.191
0.555HisGln: 0.555 ± 0.246
0.463HisArg: 0.463 ± 0.171
1.11HisSer: 1.11 ± 0.261
0.463HisThr: 0.463 ± 0.167
1.481HisVal: 1.481 ± 0.351
0.0HisTrp: 0.0 ± 0.0
0.74HisTyr: 0.74 ± 0.23
0.0HisXaa: 0.0 ± 0.0
Ile
4.904IleAla: 4.904 ± 0.918
0.37IleCys: 0.37 ± 0.184
6.847IleAsp: 6.847 ± 0.963
4.534IleGlu: 4.534 ± 0.864
1.943IlePhe: 1.943 ± 0.504
4.349IleGly: 4.349 ± 0.734
0.648IleHis: 0.648 ± 0.218
4.071IleIle: 4.071 ± 0.858
6.015IleLys: 6.015 ± 0.766
3.979IleLeu: 3.979 ± 1.039
2.128IleMet: 2.128 ± 0.427
4.997IleAsn: 4.997 ± 0.594
3.424IlePro: 3.424 ± 0.699
2.406IleGln: 2.406 ± 0.482
3.239IleArg: 3.239 ± 0.529
4.812IleSer: 4.812 ± 0.74
3.609IleThr: 3.609 ± 0.589
4.257IleVal: 4.257 ± 0.683
0.74IleTrp: 0.74 ± 0.21
2.406IleTyr: 2.406 ± 0.434
0.0IleXaa: 0.0 ± 0.0
Lys
5.83LysAla: 5.83 ± 0.7
0.555LysCys: 0.555 ± 0.296
4.164LysAsp: 4.164 ± 0.695
6.2LysGlu: 6.2 ± 0.76
3.794LysPhe: 3.794 ± 0.815
5.552LysGly: 5.552 ± 0.723
1.11LysHis: 1.11 ± 0.382
5.552LysIle: 5.552 ± 0.71
6.2LysLys: 6.2 ± 1.047
5.367LysLeu: 5.367 ± 0.632
1.758LysMet: 1.758 ± 0.454
5.459LysAsn: 5.459 ± 0.792
2.961LysPro: 2.961 ± 0.498
4.164LysGln: 4.164 ± 0.701
3.054LysArg: 3.054 ± 0.549
4.164LysSer: 4.164 ± 0.787
6.107LysThr: 6.107 ± 0.768
4.442LysVal: 4.442 ± 0.861
1.203LysTrp: 1.203 ± 0.302
2.869LysTyr: 2.869 ± 0.463
0.0LysXaa: 0.0 ± 0.0
Leu
6.385LeuAla: 6.385 ± 0.613
0.278LeuCys: 0.278 ± 0.196
5.83LeuAsp: 5.83 ± 0.748
5.83LeuGlu: 5.83 ± 1.043
2.961LeuPhe: 2.961 ± 0.456
5.552LeuGly: 5.552 ± 0.766
0.925LeuHis: 0.925 ± 0.309
4.627LeuIle: 4.627 ± 0.604
6.292LeuLys: 6.292 ± 0.716
4.904LeuLeu: 4.904 ± 0.764
2.313LeuMet: 2.313 ± 0.343
4.904LeuAsn: 4.904 ± 0.617
2.869LeuPro: 2.869 ± 0.487
2.776LeuGln: 2.776 ± 0.476
2.683LeuArg: 2.683 ± 0.479
4.997LeuSer: 4.997 ± 0.813
6.015LeuThr: 6.015 ± 0.825
3.979LeuVal: 3.979 ± 0.581
0.555LeuTrp: 0.555 ± 0.26
1.851LeuTyr: 1.851 ± 0.431
0.0LeuXaa: 0.0 ± 0.0
Met
2.591MetAla: 2.591 ± 0.572
0.093MetCys: 0.093 ± 0.085
0.74MetAsp: 0.74 ± 0.299
1.11MetGlu: 1.11 ± 0.261
1.203MetPhe: 1.203 ± 0.311
1.11MetGly: 1.11 ± 0.321
0.37MetHis: 0.37 ± 0.173
1.573MetIle: 1.573 ± 0.439
2.869MetLys: 2.869 ± 0.698
1.573MetLeu: 1.573 ± 0.273
0.833MetMet: 0.833 ± 0.312
1.388MetAsn: 1.388 ± 0.323
1.295MetPro: 1.295 ± 0.259
1.018MetGln: 1.018 ± 0.251
1.018MetArg: 1.018 ± 0.271
1.758MetSer: 1.758 ± 0.398
1.758MetThr: 1.758 ± 0.443
1.758MetVal: 1.758 ± 0.347
0.185MetTrp: 0.185 ± 0.104
1.018MetTyr: 1.018 ± 0.343
0.0MetXaa: 0.0 ± 0.0
Asn
4.904AsnAla: 4.904 ± 0.604
0.278AsnCys: 0.278 ± 0.152
3.794AsnAsp: 3.794 ± 0.67
4.627AsnGlu: 4.627 ± 0.805
2.961AsnPhe: 2.961 ± 0.605
6.477AsnGly: 6.477 ± 1.004
1.11AsnHis: 1.11 ± 0.3
3.516AsnIle: 3.516 ± 0.581
4.534AsnLys: 4.534 ± 0.74
5.367AsnLeu: 5.367 ± 0.502
0.833AsnMet: 0.833 ± 0.277
4.997AsnAsn: 4.997 ± 0.69
2.591AsnPro: 2.591 ± 0.508
2.869AsnGln: 2.869 ± 0.575
1.758AsnArg: 1.758 ± 0.472
4.719AsnSer: 4.719 ± 0.509
3.794AsnThr: 3.794 ± 0.641
3.609AsnVal: 3.609 ± 0.397
1.573AsnTrp: 1.573 ± 0.419
2.221AsnTyr: 2.221 ± 0.44
0.0AsnXaa: 0.0 ± 0.0
Pro
1.573ProAla: 1.573 ± 0.295
0.0ProCys: 0.0 ± 0.0
1.11ProAsp: 1.11 ± 0.33
2.221ProGlu: 2.221 ± 0.406
1.481ProPhe: 1.481 ± 0.372
1.203ProGly: 1.203 ± 0.45
0.463ProHis: 0.463 ± 0.195
1.481ProIle: 1.481 ± 0.387
3.146ProLys: 3.146 ± 0.478
2.776ProLeu: 2.776 ± 0.508
0.278ProMet: 0.278 ± 0.147
2.498ProAsn: 2.498 ± 0.416
0.833ProPro: 0.833 ± 0.383
1.388ProGln: 1.388 ± 0.4
1.018ProArg: 1.018 ± 0.346
2.498ProSer: 2.498 ± 0.512
2.776ProThr: 2.776 ± 0.455
2.036ProVal: 2.036 ± 0.638
0.648ProTrp: 0.648 ± 0.214
1.203ProTyr: 1.203 ± 0.49
0.0ProXaa: 0.0 ± 0.0
Gln
3.609GlnAla: 3.609 ± 0.444
0.37GlnCys: 0.37 ± 0.156
2.869GlnAsp: 2.869 ± 0.357
2.591GlnGlu: 2.591 ± 0.402
1.388GlnPhe: 1.388 ± 0.341
3.239GlnGly: 3.239 ± 0.631
0.833GlnHis: 0.833 ± 0.247
2.036GlnIle: 2.036 ± 0.309
2.776GlnLys: 2.776 ± 0.483
2.869GlnLeu: 2.869 ± 0.481
1.388GlnMet: 1.388 ± 0.299
2.313GlnAsn: 2.313 ± 0.445
0.37GlnPro: 0.37 ± 0.182
2.683GlnGln: 2.683 ± 0.505
1.481GlnArg: 1.481 ± 0.318
2.406GlnSer: 2.406 ± 0.478
2.591GlnThr: 2.591 ± 0.42
2.591GlnVal: 2.591 ± 0.578
1.11GlnTrp: 1.11 ± 0.379
2.128GlnTyr: 2.128 ± 0.541
0.0GlnXaa: 0.0 ± 0.0
Arg
2.128ArgAla: 2.128 ± 0.428
0.093ArgCys: 0.093 ± 0.1
2.961ArgAsp: 2.961 ± 0.576
2.406ArgGlu: 2.406 ± 0.464
2.036ArgPhe: 2.036 ± 0.467
2.498ArgGly: 2.498 ± 0.641
0.74ArgHis: 0.74 ± 0.228
2.683ArgIle: 2.683 ± 0.543
3.516ArgLys: 3.516 ± 0.605
2.869ArgLeu: 2.869 ± 0.704
0.833ArgMet: 0.833 ± 0.222
3.239ArgAsn: 3.239 ± 0.474
0.925ArgPro: 0.925 ± 0.326
1.573ArgGln: 1.573 ± 0.316
1.203ArgArg: 1.203 ± 0.336
1.295ArgSer: 1.295 ± 0.307
1.943ArgThr: 1.943 ± 0.57
2.591ArgVal: 2.591 ± 0.499
0.74ArgTrp: 0.74 ± 0.326
1.666ArgTyr: 1.666 ± 0.466
0.0ArgXaa: 0.0 ± 0.0
Ser
3.239SerAla: 3.239 ± 0.793
0.833SerCys: 0.833 ± 0.308
4.997SerAsp: 4.997 ± 0.651
3.609SerGlu: 3.609 ± 0.651
3.054SerPhe: 3.054 ± 0.622
4.442SerGly: 4.442 ± 0.461
0.648SerHis: 0.648 ± 0.222
4.812SerIle: 4.812 ± 0.847
4.997SerLys: 4.997 ± 0.988
3.979SerLeu: 3.979 ± 0.596
2.406SerMet: 2.406 ± 0.473
4.534SerAsn: 4.534 ± 0.596
1.758SerPro: 1.758 ± 0.39
2.776SerGln: 2.776 ± 0.5
2.406SerArg: 2.406 ± 0.503
3.794SerSer: 3.794 ± 0.702
3.886SerThr: 3.886 ± 0.497
4.719SerVal: 4.719 ± 0.705
0.833SerTrp: 0.833 ± 0.368
2.221SerTyr: 2.221 ± 0.598
0.0SerXaa: 0.0 ± 0.0
Thr
4.904ThrAla: 4.904 ± 0.524
0.278ThrCys: 0.278 ± 0.134
3.886ThrAsp: 3.886 ± 0.742
3.609ThrGlu: 3.609 ± 0.702
3.239ThrPhe: 3.239 ± 0.614
3.979ThrGly: 3.979 ± 0.572
1.11ThrHis: 1.11 ± 0.261
4.071ThrIle: 4.071 ± 0.875
4.904ThrLys: 4.904 ± 0.572
6.662ThrLeu: 6.662 ± 0.871
1.11ThrMet: 1.11 ± 0.294
4.257ThrAsn: 4.257 ± 0.751
1.481ThrPro: 1.481 ± 0.482
3.146ThrGln: 3.146 ± 0.55
1.758ThrArg: 1.758 ± 0.35
3.424ThrSer: 3.424 ± 0.624
3.609ThrThr: 3.609 ± 0.756
3.886ThrVal: 3.886 ± 0.552
1.018ThrTrp: 1.018 ± 0.246
3.424ThrTyr: 3.424 ± 0.56
0.0ThrXaa: 0.0 ± 0.0
Val
3.979ValAla: 3.979 ± 0.869
0.37ValCys: 0.37 ± 0.195
5.274ValAsp: 5.274 ± 0.521
2.961ValGlu: 2.961 ± 0.464
2.221ValPhe: 2.221 ± 0.408
4.257ValGly: 4.257 ± 0.638
0.833ValHis: 0.833 ± 0.278
4.534ValIle: 4.534 ± 0.591
4.812ValLys: 4.812 ± 0.738
4.627ValLeu: 4.627 ± 0.682
0.74ValMet: 0.74 ± 0.241
3.239ValAsn: 3.239 ± 0.556
2.036ValPro: 2.036 ± 0.41
2.128ValGln: 2.128 ± 0.485
2.128ValArg: 2.128 ± 0.461
4.997ValSer: 4.997 ± 0.583
4.627ValThr: 4.627 ± 0.736
3.331ValVal: 3.331 ± 0.607
0.74ValTrp: 0.74 ± 0.303
2.128ValTyr: 2.128 ± 0.502
0.0ValXaa: 0.0 ± 0.0
Trp
0.555TrpAla: 0.555 ± 0.229
0.185TrpCys: 0.185 ± 0.139
1.388TrpAsp: 1.388 ± 0.411
0.925TrpGlu: 0.925 ± 0.21
0.555TrpPhe: 0.555 ± 0.223
0.648TrpGly: 0.648 ± 0.297
0.37TrpHis: 0.37 ± 0.171
1.018TrpIle: 1.018 ± 0.264
1.11TrpLys: 1.11 ± 0.299
1.481TrpLeu: 1.481 ± 0.338
0.185TrpMet: 0.185 ± 0.108
0.74TrpAsn: 0.74 ± 0.334
0.0TrpPro: 0.0 ± 0.0
0.74TrpGln: 0.74 ± 0.264
0.925TrpArg: 0.925 ± 0.306
1.666TrpSer: 1.666 ± 0.65
1.388TrpThr: 1.388 ± 0.56
1.018TrpVal: 1.018 ± 0.277
0.37TrpTrp: 0.37 ± 0.202
0.278TrpTyr: 0.278 ± 0.156
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.424TyrAla: 3.424 ± 0.557
0.185TyrCys: 0.185 ± 0.132
2.313TyrAsp: 2.313 ± 0.566
2.869TyrGlu: 2.869 ± 0.65
1.943TyrPhe: 1.943 ± 0.42
1.573TyrGly: 1.573 ± 0.48
0.648TyrHis: 0.648 ± 0.239
2.961TyrIle: 2.961 ± 0.587
2.776TyrLys: 2.776 ± 0.529
3.701TyrLeu: 3.701 ± 0.578
1.018TyrMet: 1.018 ± 0.323
1.758TyrAsn: 1.758 ± 0.363
1.481TyrPro: 1.481 ± 0.39
2.406TyrGln: 2.406 ± 0.531
2.313TyrArg: 2.313 ± 0.509
2.128TyrSer: 2.128 ± 0.481
2.036TyrThr: 2.036 ± 0.668
2.776TyrVal: 2.776 ± 0.459
0.463TyrTrp: 0.463 ± 0.23
2.221TyrTyr: 2.221 ± 0.554
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 38 proteins (10808 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski