Novosphingobium aromaticivorans (strain ATCC 700278 / DSM 12444 / CCUG 56034 / CIP 105152 / NBRC 16084 / F199)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Novosphingobium; Novosphingobium aromaticivorans

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3917 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q2G7Y7|Q2G7Y7_NOVAD Type IV secretory pathway VirJ component-like protein OS=Novosphingobium aromaticivorans (strain ATCC 700278 / DSM 12444 / CCUG 56034 / CIP 105152 / NBRC 16084 / F199) OX=279238 GN=Saro_1596 PE=4 SV=1
MM1 pKa = 7.73TYY3 pKa = 10.17IQIDD7 pKa = 3.93GSLSDD12 pKa = 3.16WSSNLRR18 pKa = 11.84IDD20 pKa = 3.57AGAVDD25 pKa = 5.59GYY27 pKa = 10.82QIYY30 pKa = 8.16ATTDD34 pKa = 2.7ATDD37 pKa = 3.59YY38 pKa = 10.68IFAFAAPTAVGANTTIWLNTDD59 pKa = 3.97LNQATGYY66 pKa = 8.41QLWGTVGAEE75 pKa = 3.67FNVNFKK81 pKa = 11.01SDD83 pKa = 3.28GSAALYY89 pKa = 10.58SGAAGGTLVADD100 pKa = 4.11NLVLAYY106 pKa = 10.55NADD109 pKa = 3.26KK110 pKa = 11.27TMVEE114 pKa = 3.9LRR116 pKa = 11.84VPKK119 pKa = 10.37DD120 pKa = 3.17LLGNPGSIDD129 pKa = 3.25TVYY132 pKa = 10.73DD133 pKa = 3.49INDD136 pKa = 3.58TAIIPSFYY144 pKa = 9.67QDD146 pKa = 2.75NAIRR150 pKa = 11.84VWDD153 pKa = 4.28DD154 pKa = 3.47SEE156 pKa = 4.19LASVIPATDD165 pKa = 3.06TRR167 pKa = 11.84IAIVYY172 pKa = 9.57SATTAANYY180 pKa = 9.34FSQTAYY186 pKa = 11.1SDD188 pKa = 3.91LFMAAQSQAAQAGVPFDD205 pKa = 4.93IITEE209 pKa = 4.12ADD211 pKa = 3.25LTDD214 pKa = 3.57INKK217 pKa = 9.48LAQYY221 pKa = 9.69KK222 pKa = 10.62AIVFPSFRR230 pKa = 11.84NVQASQADD238 pKa = 4.58EE239 pKa = 4.11IAHH242 pKa = 5.93TLQLASQEE250 pKa = 4.06FHH252 pKa = 6.59VGFIVSGEE260 pKa = 3.82FMTNDD265 pKa = 3.2EE266 pKa = 5.1NGNAMAGNSYY276 pKa = 11.15SRR278 pKa = 11.84MATLLDD284 pKa = 3.43ATRR287 pKa = 11.84VTGGTATSLTVTATDD302 pKa = 3.54PTGVVLDD309 pKa = 5.13GYY311 pKa = 11.83ANGEE315 pKa = 4.33LVNQYY320 pKa = 11.8ANVGWNAFQSVSGTGQTIATEE341 pKa = 4.62TINGSSTYY349 pKa = 10.72AAVLATQTGGRR360 pKa = 11.84NVLFSSDD367 pKa = 3.76AVMADD372 pKa = 2.83ANMLQRR378 pKa = 11.84AIDD381 pKa = 3.77YY382 pKa = 9.16AVSGEE387 pKa = 4.34TVTVSLNMTRR397 pKa = 11.84DD398 pKa = 3.35AGLVAARR405 pKa = 11.84VDD407 pKa = 4.17MDD409 pKa = 3.04QSMYY413 pKa = 10.59IEE415 pKa = 4.8DD416 pKa = 4.26VNGGIYY422 pKa = 10.18DD423 pKa = 3.51QLVPLLQQWKK433 pKa = 8.6AQYY436 pKa = 10.58NFVGSFYY443 pKa = 11.61VNIGDD448 pKa = 3.69NTQQGIYY455 pKa = 9.54TDD457 pKa = 3.72WNKK460 pKa = 10.79SLPYY464 pKa = 7.38YY465 pKa = 9.85TAMIGLGNEE474 pKa = 4.32IGTHH478 pKa = 5.99TYY480 pKa = 7.58THH482 pKa = 7.2PEE484 pKa = 4.0DD485 pKa = 4.12TNLLSPSQLQFEE497 pKa = 5.18FEE499 pKa = 4.67LSTQILEE506 pKa = 4.46QKK508 pKa = 10.55LSAALGYY515 pKa = 10.71AYY517 pKa = 9.2TIEE520 pKa = 4.38GAAIPGAPEE529 pKa = 3.76TLTTSLAIEE538 pKa = 4.42QYY540 pKa = 10.6VKK542 pKa = 10.12TYY544 pKa = 9.4LTGGYY549 pKa = 7.65TGQGAGYY556 pKa = 8.58PNAFGYY562 pKa = 7.44LTPGSQDD569 pKa = 2.87KK570 pKa = 11.23VYY572 pKa = 10.35IAPNTFFDD580 pKa = 3.82FTLFDD585 pKa = 3.94WLHH588 pKa = 6.45LSAADD593 pKa = 5.8ASALWQSQYY602 pKa = 11.47EE603 pKa = 4.5KK604 pKa = 10.34IVSQADD610 pKa = 3.66SPVVVWPWHH619 pKa = 7.13DD620 pKa = 3.77YY621 pKa = 10.79GATAFNSPNYY631 pKa = 10.21APEE634 pKa = 3.94IFNTFLAQAAADD646 pKa = 3.45GMEE649 pKa = 4.15FVTLADD655 pKa = 3.54LANRR659 pKa = 11.84INAFHH664 pKa = 6.57GAKK667 pKa = 8.86VTTSVSGNTITANVTASGNVGTFAFDD693 pKa = 3.44LQGQGSQVISSVAGWYY709 pKa = 10.19AYY711 pKa = 10.58DD712 pKa = 3.7SDD714 pKa = 5.71SVFLPQNGGTFVITLGAAQTDD735 pKa = 3.78VTHH738 pKa = 7.57IIDD741 pKa = 3.89LPMRR745 pKa = 11.84ATLMSVTGNGTNLSFQIQGEE765 pKa = 4.55GTVVIDD771 pKa = 6.01LSDD774 pKa = 3.7PTNKK778 pKa = 9.83SVQVSGATIVSQVGDD793 pKa = 4.23KK794 pKa = 10.05LTIDD798 pKa = 3.65IGPVGSHH805 pKa = 5.33TVTVTQTSLNHH816 pKa = 6.16APVIEE821 pKa = 4.53SNGGGDD827 pKa = 3.26TAAISLAEE835 pKa = 4.12NLLAVTAVIATDD847 pKa = 3.36ADD849 pKa = 4.17ANALTYY855 pKa = 10.51SITGGADD862 pKa = 2.91ASKK865 pKa = 9.22FTINATTGALAFLAAPNFEE884 pKa = 4.22VPTDD888 pKa = 3.48VGGNNVYY895 pKa = 10.74DD896 pKa = 4.03VVVTASDD903 pKa = 3.74GALTDD908 pKa = 3.8SQALAVTVTNVNEE921 pKa = 4.19APVITSNGGGATASISLAEE940 pKa = 4.28NNAAVTVVTSTDD952 pKa = 3.46PEE954 pKa = 4.18NTARR958 pKa = 11.84TYY960 pKa = 11.27SLSGTDD966 pKa = 3.19AARR969 pKa = 11.84FTIDD973 pKa = 3.17AATGALSFVNAPDD986 pKa = 3.9FEE988 pKa = 4.99NPTDD992 pKa = 3.45VGANNVYY999 pKa = 10.51NVVVTASDD1007 pKa = 3.66GSLTDD1012 pKa = 3.57TQALAITVTNKK1023 pKa = 10.46KK1024 pKa = 10.3GVTLNASSSTGSVLNGTGEE1043 pKa = 4.08EE1044 pKa = 4.09DD1045 pKa = 4.73QLNGWKK1051 pKa = 10.49GADD1054 pKa = 3.21TLYY1057 pKa = 11.48GLGGNDD1063 pKa = 3.48RR1064 pKa = 11.84LDD1066 pKa = 3.75GAGGNDD1072 pKa = 3.2RR1073 pKa = 11.84LYY1075 pKa = 11.23GGDD1078 pKa = 3.83GKK1080 pKa = 10.73DD1081 pKa = 3.51VLIGGAGTDD1090 pKa = 3.42IMSGGAGADD1099 pKa = 2.72RR1100 pKa = 11.84FEE1102 pKa = 5.04FNALGNSVTGALHH1115 pKa = 7.39DD1116 pKa = 4.53VITDD1120 pKa = 3.81FEE1122 pKa = 5.38AGIDD1126 pKa = 4.09LIDD1129 pKa = 4.14VSSIDD1134 pKa = 3.66ANSGKK1139 pKa = 10.5GGNQTFVLLAEE1150 pKa = 4.95GAAFTGVGQLRR1161 pKa = 11.84YY1162 pKa = 9.25FYY1164 pKa = 11.04DD1165 pKa = 3.41SATDD1169 pKa = 3.41QTIVQGNVNNNLAADD1184 pKa = 4.03FEE1186 pKa = 4.78IALSGHH1192 pKa = 4.87QTLSASMFILL1202 pKa = 4.18

Molecular weight: 125.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q2GAX6|Q2GAX6_NOVAD GMP synthase [glutamine-hydrolyzing] OS=Novosphingobium aromaticivorans (strain ATCC 700278 / DSM 12444 / CCUG 56034 / CIP 105152 / NBRC 16084 / F199) OX=279238 GN=guaA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84LVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.54GFRR19 pKa = 11.84ARR21 pKa = 11.84TATVGGRR28 pKa = 11.84KK29 pKa = 9.07VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3917

0

3917

1285425

41

3069

328.2

35.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.189 ± 0.047

0.877 ± 0.013

5.824 ± 0.027

5.649 ± 0.032

3.611 ± 0.024

9.085 ± 0.052

2.014 ± 0.02

4.683 ± 0.022

3.035 ± 0.033

9.727 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.486 ± 0.02

2.613 ± 0.023

5.324 ± 0.028

3.053 ± 0.02

7.303 ± 0.041

5.272 ± 0.033

5.181 ± 0.029

7.399 ± 0.029

1.484 ± 0.018

2.191 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski