Sinomonas atrocyanea
Average proteome isoelectric point is 6.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4104 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A126ZXV7|A0A126ZXV7_9MICC 5-carboxymethyl-2-hydroxymuconate delta-isomerase OS=Sinomonas atrocyanea OX=37927 GN=SA2016_1328 PE=4 SV=1
MM1 pKa = 7.46 SKK3 pKa = 9.95 VQTGLRR9 pKa = 11.84 YY10 pKa = 9.56 SEE12 pKa = 3.92 EE13 pKa = 4.39 HH14 pKa = 6.05 EE15 pKa = 4.34 WLDD18 pKa = 3.86 TDD20 pKa = 5.01 AAPAKK25 pKa = 10.26 IGVSQVAADD34 pKa = 3.67 ALGDD38 pKa = 3.87 VVYY41 pKa = 11.05 LDD43 pKa = 4.31 LPEE46 pKa = 5.62 AGSTVSAGQTCGEE59 pKa = 4.14 IEE61 pKa = 4.32 STKK64 pKa = 10.64 SVSDD68 pKa = 4.02 LYY70 pKa = 11.42 SPVSGTVVEE79 pKa = 4.83 VNQEE83 pKa = 4.38 AIDD86 pKa = 4.04 NPALVNEE93 pKa = 4.6 DD94 pKa = 3.86 PYY96 pKa = 11.2 GAGWLFTVEE105 pKa = 4.15 VSEE108 pKa = 4.65 EE109 pKa = 4.41 GPLMSAEE116 pKa = 4.25 EE117 pKa = 4.12 YY118 pKa = 10.43 AGKK121 pKa = 10.26 NGGEE125 pKa = 4.13 LL126 pKa = 3.47
Molecular weight: 13.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.778
IPC2_protein 3.834
IPC_protein 3.745
Toseland 3.579
ProMoST 3.884
Dawson 3.706
Bjellqvist 3.872
Wikipedia 3.605
Rodwell 3.592
Grimsley 3.49
Solomon 3.694
Lehninger 3.643
Nozaki 3.834
DTASelect 3.961
Thurlkill 3.617
EMBOSS 3.617
Sillero 3.859
Patrickios 1.825
IPC_peptide 3.694
IPC2_peptide 3.846
IPC2.peptide.svr19 3.779
Protein with the highest isoelectric point:
>tr|A0A127A218|A0A127A218_9MICC Putative cold shock protein OS=Sinomonas atrocyanea OX=37927 GN=SA2016_1007 PE=4 SV=1
MM1 pKa = 7.61 KK2 pKa = 10.31 VRR4 pKa = 11.84 NSLRR8 pKa = 11.84 ALKK11 pKa = 9.68 KK12 pKa = 9.79 VPGAQVVRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 GRR24 pKa = 11.84 TFVINKK30 pKa = 9.04 RR31 pKa = 11.84 SPRR34 pKa = 11.84 LSARR38 pKa = 11.84 QGG40 pKa = 3.13
Molecular weight: 4.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.508
IPC2_protein 11.155
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.91
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.574
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.31
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.145
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4104
0
4104
1325928
29
2174
323.1
34.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.516 ± 0.054
0.637 ± 0.01
5.437 ± 0.031
5.743 ± 0.042
3.026 ± 0.024
9.57 ± 0.034
2.15 ± 0.018
3.801 ± 0.027
2.148 ± 0.025
10.183 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.82 ± 0.014
1.895 ± 0.02
5.795 ± 0.031
2.891 ± 0.02
7.287 ± 0.042
5.506 ± 0.032
5.607 ± 0.041
8.481 ± 0.04
1.471 ± 0.016
2.037 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here