ANMV-1 virus

Taxonomy: Viruses; unclassified archaeal viruses

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5KMN7|A0A0C5KMN7_9VIRU Uncharacterized protein OS=ANMV-1 virus OX=1605378 PE=4 SV=1
MM1 pKa = 7.97DD2 pKa = 5.53PAPGKK7 pKa = 10.52VEE9 pKa = 4.02ADD11 pKa = 3.38RR12 pKa = 11.84AGWFVRR18 pKa = 11.84DD19 pKa = 3.65YY20 pKa = 10.97FPDD23 pKa = 3.65GVAPVSKK30 pKa = 10.27ILFDD34 pKa = 3.92GAGSGTGIDD43 pKa = 3.94FASTAFTTGIDD54 pKa = 3.24ISGTCVTGISMTNASAVGILVKK76 pKa = 10.46AGPMGFDD83 pKa = 2.95ITSTIPAGAATNANEE98 pKa = 5.15LNLTDD103 pKa = 5.36DD104 pKa = 4.07STGSSGLARR113 pKa = 11.84ALWINAVVAGDD124 pKa = 3.45KK125 pKa = 10.62TGTGEE130 pKa = 4.21HH131 pKa = 6.49NSFGIDD137 pKa = 3.15QHH139 pKa = 5.4VTGDD143 pKa = 3.5TPYY146 pKa = 10.65LYY148 pKa = 11.29GMTYY152 pKa = 10.49YY153 pKa = 10.66SYY155 pKa = 10.2DD156 pKa = 3.33TGDD159 pKa = 3.43PTIGFAAPISIYY171 pKa = 10.47QDD173 pKa = 3.5DD174 pKa = 4.63LGTNIGAYY182 pKa = 9.83VGIDD186 pKa = 3.0IGIALSNPPTDD197 pKa = 3.51RR198 pKa = 11.84YY199 pKa = 10.55SFMRR203 pKa = 11.84FRR205 pKa = 11.84EE206 pKa = 3.94HH207 pKa = 5.63STAVPKK213 pKa = 10.76SVFRR217 pKa = 11.84FEE219 pKa = 4.39GAHH222 pKa = 5.98CASHH226 pKa = 6.86LFDD229 pKa = 3.57VTAGTGMPDD238 pKa = 4.74FITTEE243 pKa = 3.79AGATFAGDD251 pKa = 4.14GIKK254 pKa = 10.05IAVEE258 pKa = 4.2YY259 pKa = 10.82QGTTYY264 pKa = 11.16YY265 pKa = 10.94LRR267 pKa = 11.84ACSAFSS273 pKa = 3.19

Molecular weight: 28.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5K865|A0A0C5K865_9VIRU Uncharacterized protein OS=ANMV-1 virus OX=1605378 PE=4 SV=1
MM1 pKa = 7.8GEE3 pKa = 4.97RR4 pKa = 11.84YY5 pKa = 9.99YY6 pKa = 10.92KK7 pKa = 10.32WEE9 pKa = 4.19DD10 pKa = 3.15ALIIKK15 pKa = 7.95YY16 pKa = 10.11YY17 pKa = 8.27PTAARR22 pKa = 11.84EE23 pKa = 3.95KK24 pKa = 9.01MLEE27 pKa = 4.08HH28 pKa = 6.41VPTRR32 pKa = 11.84TWSQIGLRR40 pKa = 11.84ARR42 pKa = 11.84ALGIHH47 pKa = 6.21RR48 pKa = 11.84TTKK51 pKa = 10.37AWGDD55 pKa = 3.86SIRR58 pKa = 11.84EE59 pKa = 3.97GRR61 pKa = 11.84KK62 pKa = 9.4GLRR65 pKa = 11.84GTWDD69 pKa = 3.35DD70 pKa = 3.58VDD72 pKa = 4.37NEE74 pKa = 4.4RR75 pKa = 11.84LDD77 pKa = 3.69YY78 pKa = 9.91MYY80 pKa = 8.26PTATRR85 pKa = 11.84AQLRR89 pKa = 11.84VAFPSRR95 pKa = 11.84TWLSLQSHH103 pKa = 5.41AQKK106 pKa = 10.62RR107 pKa = 11.84HH108 pKa = 3.5IHH110 pKa = 5.05RR111 pKa = 11.84TRR113 pKa = 11.84EE114 pKa = 3.8AVGRR118 pKa = 11.84QISIGRR124 pKa = 11.84RR125 pKa = 11.84DD126 pKa = 3.35GRR128 pKa = 11.84KK129 pKa = 9.54NEE131 pKa = 3.88

Molecular weight: 15.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

69

0

69

11327

37

1166

164.2

18.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.01 ± 0.456

2.702 ± 1.023

6.551 ± 0.404

8.14 ± 0.5

3.69 ± 0.304

6.833 ± 0.375

2.693 ± 0.213

6.224 ± 0.353

7.292 ± 0.517

7.637 ± 0.482

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.092 ± 0.146

4.043 ± 0.256

3.893 ± 0.206

2.86 ± 0.177

5.747 ± 0.379

5.315 ± 0.275

5.862 ± 0.449

6.145 ± 0.308

1.571 ± 0.142

3.699 ± 0.191
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski