Emiliania huxleyi virus 99B1

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Algavirales; Phycodnaviridae; Coccolithovirus; unclassified Coccolithovirus

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 444 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D2TFC0|D2TFC0_9PHYC Putative C2H2-type Zinc finger protein OS=Emiliania huxleyi virus 99B1 OX=187397 GN=EhV184A PE=4 SV=1
MM1 pKa = 7.46LSKK4 pKa = 10.26FIHH7 pKa = 5.8MFCIGVSIAGYY18 pKa = 9.44PSGYY22 pKa = 9.55YY23 pKa = 10.19NRR25 pKa = 11.84LTPNAPNVGTFHH37 pKa = 6.49GTCTCPDD44 pKa = 3.17GSVYY48 pKa = 10.43IVGDD52 pKa = 3.51NVNQCGSLACYY63 pKa = 10.07GGVSGTCHH71 pKa = 6.81KK72 pKa = 11.22SNVAMDD78 pKa = 5.02HH79 pKa = 5.91IQGLMVEE86 pKa = 4.85CNVVAPSPPPSPPPSSPPSPSPPPSPLAPGHH117 pKa = 7.04DD118 pKa = 4.18YY119 pKa = 11.87VEE121 pKa = 4.52MFTSNISTSFIIAEE135 pKa = 4.69PDD137 pKa = 3.81LIDD140 pKa = 4.53PGSGSEE146 pKa = 3.85IGSGEE151 pKa = 3.88GRR153 pKa = 11.84RR154 pKa = 11.84LLEE157 pKa = 3.88TAAISPLVFEE167 pKa = 4.56YY168 pKa = 11.37AMYY171 pKa = 8.81TAFRR175 pKa = 11.84IMYY178 pKa = 7.24NVPRR182 pKa = 11.84FHH184 pKa = 7.65PDD186 pKa = 2.65CGLIEE191 pKa = 4.8SISNKK196 pKa = 10.17CIDD199 pKa = 3.25IMYY202 pKa = 10.67DD203 pKa = 3.27EE204 pKa = 5.27DD205 pKa = 4.07TTNSSVKK212 pKa = 10.37VCISPNMNSGDD223 pKa = 3.71TSITDD228 pKa = 3.66ALSTEE233 pKa = 4.41SGADD237 pKa = 3.66PIEE240 pKa = 4.97AIVDD244 pKa = 3.68IFNKK248 pKa = 10.13IIIGEE253 pKa = 4.23DD254 pKa = 3.02VGSSFFEE261 pKa = 4.22VSTIDD266 pKa = 3.98VPADD270 pKa = 3.81LYY272 pKa = 11.54AAFIATDD279 pKa = 3.8DD280 pKa = 4.13NNTLIYY286 pKa = 10.61NISTFTNRR294 pKa = 11.84YY295 pKa = 5.38NTEE298 pKa = 4.24PYY300 pKa = 10.81DD301 pKa = 5.01LDD303 pKa = 5.75DD304 pKa = 5.53PDD306 pKa = 3.68VTITFINPTSTQIVNKK322 pKa = 9.7PADD325 pKa = 4.79DD326 pKa = 6.11DD327 pKa = 6.37DD328 pKa = 7.46DD329 pKa = 7.38DD330 pKa = 7.43DD331 pKa = 7.58DD332 pKa = 7.0DD333 pKa = 4.9GLSDD337 pKa = 4.17GDD339 pKa = 3.68VAGIIIAAIIGLMFILIIAFVMWPKK364 pKa = 10.58NSRR367 pKa = 11.84VCVRR371 pKa = 11.84RR372 pKa = 11.84DD373 pKa = 3.32PEE375 pKa = 4.53VISTTPTPGLEE386 pKa = 4.28TVSPPTEE393 pKa = 3.97RR394 pKa = 11.84EE395 pKa = 3.82ITVATQPSAEE405 pKa = 4.16VTEE408 pKa = 4.36ITMTEE413 pKa = 3.99PP414 pKa = 3.64

Molecular weight: 44.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D2TFC3|D2TFC3_9PHYC Putative membrane protein OS=Emiliania huxleyi virus 99B1 OX=187397 GN=EhV187 PE=4 SV=1
MM1 pKa = 7.25AVSRR5 pKa = 11.84RR6 pKa = 11.84SRR8 pKa = 11.84YY9 pKa = 7.36GTVRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84TVRR20 pKa = 11.84KK21 pKa = 8.12STASRR26 pKa = 11.84STKK29 pKa = 9.49VGRR32 pKa = 11.84VAKK35 pKa = 9.99LSNMSAGCRR44 pKa = 11.84RR45 pKa = 11.84YY46 pKa = 10.33VKK48 pKa = 10.58SKK50 pKa = 10.47LGSRR54 pKa = 11.84KK55 pKa = 9.56LSRR58 pKa = 11.84ATRR61 pKa = 11.84ASIIRR66 pKa = 11.84SSVKK70 pKa = 8.95SWKK73 pKa = 10.17RR74 pKa = 11.84MSKK77 pKa = 7.59TRR79 pKa = 11.84KK80 pKa = 8.61AAYY83 pKa = 9.3SKK85 pKa = 10.35RR86 pKa = 11.84GRR88 pKa = 11.84VSTRR92 pKa = 11.84SRR94 pKa = 11.84RR95 pKa = 11.84RR96 pKa = 11.84STKK99 pKa = 8.68RR100 pKa = 11.84SRR102 pKa = 11.84RR103 pKa = 11.84TRR105 pKa = 11.84TKK107 pKa = 10.01RR108 pKa = 11.84RR109 pKa = 11.84RR110 pKa = 11.84STKK113 pKa = 9.41RR114 pKa = 11.84SRR116 pKa = 11.84RR117 pKa = 11.84SSKK120 pKa = 9.88KK121 pKa = 9.43SRR123 pKa = 11.84RR124 pKa = 11.84STMKK128 pKa = 9.74RR129 pKa = 11.84PKK131 pKa = 10.03VSTMSKK137 pKa = 10.13SLKK140 pKa = 10.19KK141 pKa = 10.61YY142 pKa = 10.13IRR144 pKa = 11.84TKK146 pKa = 10.44SGKK149 pKa = 9.72KK150 pKa = 9.61NLSKK154 pKa = 11.04VSTKK158 pKa = 7.7MLRR161 pKa = 11.84KK162 pKa = 9.62LVALWRR168 pKa = 11.84RR169 pKa = 11.84MSAKK173 pKa = 10.19SKK175 pKa = 10.43AKK177 pKa = 9.76YY178 pKa = 9.51SVRR181 pKa = 11.84STRR184 pKa = 11.84KK185 pKa = 6.64STRR188 pKa = 11.84RR189 pKa = 11.84KK190 pKa = 6.68STRR193 pKa = 11.84RR194 pKa = 11.84KK195 pKa = 6.67STRR198 pKa = 11.84RR199 pKa = 11.84KK200 pKa = 7.3STRR203 pKa = 11.84KK204 pKa = 8.76RR205 pKa = 11.84RR206 pKa = 11.84RR207 pKa = 11.84SRR209 pKa = 11.84RR210 pKa = 11.84RR211 pKa = 11.84SRR213 pKa = 11.84PRR215 pKa = 11.84TRR217 pKa = 11.84RR218 pKa = 11.84VRR220 pKa = 11.84RR221 pKa = 11.84TRR223 pKa = 11.84TRR225 pKa = 11.84RR226 pKa = 11.84TKK228 pKa = 8.45TRR230 pKa = 11.84RR231 pKa = 11.84TRR233 pKa = 11.84RR234 pKa = 11.84TVRR237 pKa = 11.84RR238 pKa = 11.84RR239 pKa = 3.12

Molecular weight: 28.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

440

4

444

110970

8

1994

249.9

28.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.421 ± 0.114

1.814 ± 0.071

5.857 ± 0.1

5.146 ± 0.117

3.897 ± 0.075

5.275 ± 0.121

2.633 ± 0.07

7.34 ± 0.132

5.839 ± 0.152

7.471 ± 0.109

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.122 ± 0.076

5.626 ± 0.09

5.474 ± 0.305

3.09 ± 0.062

4.808 ± 0.111

7.295 ± 0.127

7.017 ± 0.095

6.506 ± 0.104

1.075 ± 0.039

4.295 ± 0.089
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski