Amino acid dipepetide frequency for Pseudomonas phage Psa21

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.155AlaAla: 7.155 ± 0.448
0.569AlaCys: 0.569 ± 0.08
4.531AlaAsp: 4.531 ± 0.206
4.921AlaGlu: 4.921 ± 0.27
2.971AlaPhe: 2.971 ± 0.176
5.058AlaGly: 5.058 ± 0.411
1.444AlaHis: 1.444 ± 0.124
4.879AlaIle: 4.879 ± 0.239
4.552AlaLys: 4.552 ± 0.275
7.165AlaLeu: 7.165 ± 0.276
2.729AlaMet: 2.729 ± 0.16
3.804AlaAsn: 3.804 ± 0.221
2.824AlaPro: 2.824 ± 0.167
3.224AlaGln: 3.224 ± 0.198
3.435AlaArg: 3.435 ± 0.204
4.499AlaSer: 4.499 ± 0.317
5.026AlaThr: 5.026 ± 0.258
5.542AlaVal: 5.542 ± 0.272
1.033AlaTrp: 1.033 ± 0.131
2.782AlaTyr: 2.782 ± 0.229
0.0AlaXaa: 0.0 ± 0.0
Cys
0.601CysAla: 0.601 ± 0.086
0.148CysCys: 0.148 ± 0.035
0.558CysAsp: 0.558 ± 0.086
0.653CysGlu: 0.653 ± 0.082
0.348CysPhe: 0.348 ± 0.06
0.569CysGly: 0.569 ± 0.077
0.306CysHis: 0.306 ± 0.066
0.527CysIle: 0.527 ± 0.068
0.516CysLys: 0.516 ± 0.084
0.685CysLeu: 0.685 ± 0.094
0.242CysMet: 0.242 ± 0.046
0.506CysAsn: 0.506 ± 0.082
0.358CysPro: 0.358 ± 0.074
0.327CysGln: 0.327 ± 0.058
0.4CysArg: 0.4 ± 0.064
0.632CysSer: 0.632 ± 0.088
0.464CysThr: 0.464 ± 0.071
0.727CysVal: 0.727 ± 0.091
0.19CysTrp: 0.19 ± 0.04
0.337CysTyr: 0.337 ± 0.07
0.0CysXaa: 0.0 ± 0.0
Asp
4.942AspAla: 4.942 ± 0.243
0.495AspCys: 0.495 ± 0.075
3.509AspAsp: 3.509 ± 0.187
4.025AspGlu: 4.025 ± 0.216
2.603AspPhe: 2.603 ± 0.169
4.415AspGly: 4.415 ± 0.509
1.328AspHis: 1.328 ± 0.111
3.856AspIle: 3.856 ± 0.209
3.456AspLys: 3.456 ± 0.171
5.732AspLeu: 5.732 ± 0.217
1.76AspMet: 1.76 ± 0.126
2.877AspAsn: 2.877 ± 0.201
3.119AspPro: 3.119 ± 0.187
2.339AspGln: 2.339 ± 0.187
3.045AspArg: 3.045 ± 0.22
3.34AspSer: 3.34 ± 0.186
3.656AspThr: 3.656 ± 0.172
4.267AspVal: 4.267 ± 0.235
1.043AspTrp: 1.043 ± 0.098
2.634AspTyr: 2.634 ± 0.199
0.0AspXaa: 0.0 ± 0.0
Glu
5.574GluAla: 5.574 ± 0.272
0.516GluCys: 0.516 ± 0.077
3.625GluAsp: 3.625 ± 0.221
4.678GluGlu: 4.678 ± 0.332
2.771GluPhe: 2.771 ± 0.191
3.309GluGly: 3.309 ± 0.192
1.57GluHis: 1.57 ± 0.136
4.12GluIle: 4.12 ± 0.208
3.372GluLys: 3.372 ± 0.218
6.87GluLeu: 6.87 ± 0.263
1.918GluMet: 1.918 ± 0.132
2.413GluAsn: 2.413 ± 0.186
2.308GluPro: 2.308 ± 0.154
3.151GluGln: 3.151 ± 0.183
3.467GluArg: 3.467 ± 0.217
3.435GluSer: 3.435 ± 0.2
3.561GluThr: 3.561 ± 0.22
4.489GluVal: 4.489 ± 0.223
1.412GluTrp: 1.412 ± 0.142
2.402GluTyr: 2.402 ± 0.163
0.0GluXaa: 0.0 ± 0.0
Phe
2.603PheAla: 2.603 ± 0.157
0.337PheCys: 0.337 ± 0.064
3.056PheAsp: 3.056 ± 0.197
2.286PheGlu: 2.286 ± 0.148
1.654PhePhe: 1.654 ± 0.155
2.392PheGly: 2.392 ± 0.156
0.853PheHis: 0.853 ± 0.117
2.455PheIle: 2.455 ± 0.17
2.371PheLys: 2.371 ± 0.162
2.971PheLeu: 2.971 ± 0.196
1.138PheMet: 1.138 ± 0.108
2.139PheAsn: 2.139 ± 0.159
1.686PhePro: 1.686 ± 0.125
1.665PheGln: 1.665 ± 0.145
1.949PheArg: 1.949 ± 0.132
2.381PheSer: 2.381 ± 0.143
2.971PheThr: 2.971 ± 0.185
2.666PheVal: 2.666 ± 0.153
0.421PheTrp: 0.421 ± 0.067
1.38PheTyr: 1.38 ± 0.127
0.0PheXaa: 0.0 ± 0.0
Gly
4.025GlyAla: 4.025 ± 0.324
0.558GlyCys: 0.558 ± 0.075
3.951GlyAsp: 3.951 ± 0.282
4.152GlyGlu: 4.152 ± 0.28
2.719GlyPhe: 2.719 ± 0.172
3.772GlyGly: 3.772 ± 0.301
1.106GlyHis: 1.106 ± 0.122
3.793GlyIle: 3.793 ± 0.223
4.141GlyLys: 4.141 ± 0.312
4.931GlyLeu: 4.931 ± 0.218
2.044GlyMet: 2.044 ± 0.152
3.319GlyAsn: 3.319 ± 0.22
1.981GlyPro: 1.981 ± 0.18
2.297GlyGln: 2.297 ± 0.249
3.003GlyArg: 3.003 ± 0.212
3.73GlySer: 3.73 ± 0.287
4.288GlyThr: 4.288 ± 0.411
4.425GlyVal: 4.425 ± 0.231
0.917GlyTrp: 0.917 ± 0.09
2.613GlyTyr: 2.613 ± 0.144
0.0GlyXaa: 0.0 ± 0.0
His
1.401HisAla: 1.401 ± 0.129
0.263HisCys: 0.263 ± 0.058
1.264HisAsp: 1.264 ± 0.12
1.159HisGlu: 1.159 ± 0.119
1.012HisPhe: 1.012 ± 0.095
1.496HisGly: 1.496 ± 0.132
0.674HisHis: 0.674 ± 0.088
1.285HisIle: 1.285 ± 0.129
0.885HisLys: 0.885 ± 0.112
2.118HisLeu: 2.118 ± 0.148
0.548HisMet: 0.548 ± 0.082
1.106HisAsn: 1.106 ± 0.12
1.17HisPro: 1.17 ± 0.127
0.864HisGln: 0.864 ± 0.114
1.38HisArg: 1.38 ± 0.121
1.254HisSer: 1.254 ± 0.114
1.254HisThr: 1.254 ± 0.127
1.76HisVal: 1.76 ± 0.14
0.379HisTrp: 0.379 ± 0.065
1.138HisTyr: 1.138 ± 0.111
0.0HisXaa: 0.0 ± 0.0
Ile
4.836IleAla: 4.836 ± 0.236
0.622IleCys: 0.622 ± 0.083
4.626IleAsp: 4.626 ± 0.24
4.13IleGlu: 4.13 ± 0.219
1.538IlePhe: 1.538 ± 0.155
3.551IleGly: 3.551 ± 0.198
1.391IleHis: 1.391 ± 0.129
2.845IleIle: 2.845 ± 0.176
3.351IleLys: 3.351 ± 0.187
4.067IleLeu: 4.067 ± 0.211
1.349IleMet: 1.349 ± 0.124
3.561IleAsn: 3.561 ± 0.199
3.351IlePro: 3.351 ± 0.204
2.466IleGln: 2.466 ± 0.149
3.635IleArg: 3.635 ± 0.188
3.372IleSer: 3.372 ± 0.227
4.415IleThr: 4.415 ± 0.252
4.573IleVal: 4.573 ± 0.258
0.79IleTrp: 0.79 ± 0.095
1.654IleTyr: 1.654 ± 0.139
0.0IleXaa: 0.0 ± 0.0
Lys
4.805LysAla: 4.805 ± 0.299
0.443LysCys: 0.443 ± 0.063
3.561LysAsp: 3.561 ± 0.211
4.404LysGlu: 4.404 ± 0.249
1.854LysPhe: 1.854 ± 0.136
3.161LysGly: 3.161 ± 0.32
1.591LysHis: 1.591 ± 0.136
3.003LysIle: 3.003 ± 0.172
3.161LysLys: 3.161 ± 0.211
5.395LysLeu: 5.395 ± 0.263
1.612LysMet: 1.612 ± 0.142
2.244LysAsn: 2.244 ± 0.167
2.571LysPro: 2.571 ± 0.187
2.624LysGln: 2.624 ± 0.165
3.129LysArg: 3.129 ± 0.181
2.719LysSer: 2.719 ± 0.195
3.309LysThr: 3.309 ± 0.206
4.025LysVal: 4.025 ± 0.192
0.896LysTrp: 0.896 ± 0.096
2.034LysTyr: 2.034 ± 0.148
0.0LysXaa: 0.0 ± 0.0
Leu
7.418LeuAla: 7.418 ± 0.286
1.001LeuCys: 1.001 ± 0.115
6.164LeuAsp: 6.164 ± 0.251
5.89LeuGlu: 5.89 ± 0.217
3.224LeuPhe: 3.224 ± 0.187
5.247LeuGly: 5.247 ± 0.204
2.086LeuHis: 2.086 ± 0.193
4.815LeuIle: 4.815 ± 0.233
5.353LeuLys: 5.353 ± 0.263
6.775LeuLeu: 6.775 ± 0.304
2.318LeuMet: 2.318 ± 0.155
4.415LeuAsn: 4.415 ± 0.231
3.772LeuPro: 3.772 ± 0.224
3.361LeuGln: 3.361 ± 0.169
4.478LeuArg: 4.478 ± 0.252
5.163LeuSer: 5.163 ± 0.24
5.247LeuThr: 5.247 ± 0.229
5.932LeuVal: 5.932 ± 0.253
0.885LeuTrp: 0.885 ± 0.116
2.634LeuTyr: 2.634 ± 0.185
0.0LeuXaa: 0.0 ± 0.0
Met
2.719MetAla: 2.719 ± 0.211
0.263MetCys: 0.263 ± 0.056
1.686MetAsp: 1.686 ± 0.115
1.559MetGlu: 1.559 ± 0.137
1.296MetPhe: 1.296 ± 0.14
1.823MetGly: 1.823 ± 0.136
0.674MetHis: 0.674 ± 0.093
1.507MetIle: 1.507 ± 0.133
1.328MetLys: 1.328 ± 0.1
2.423MetLeu: 2.423 ± 0.165
0.695MetMet: 0.695 ± 0.098
1.623MetAsn: 1.623 ± 0.113
1.254MetPro: 1.254 ± 0.123
1.264MetGln: 1.264 ± 0.124
1.559MetArg: 1.559 ± 0.129
2.308MetSer: 2.308 ± 0.14
2.044MetThr: 2.044 ± 0.164
1.781MetVal: 1.781 ± 0.144
0.179MetTrp: 0.179 ± 0.039
1.001MetTyr: 1.001 ± 0.125
0.0MetXaa: 0.0 ± 0.0
Asn
3.361AsnAla: 3.361 ± 0.199
0.411AsnCys: 0.411 ± 0.065
2.761AsnAsp: 2.761 ± 0.18
3.098AsnGlu: 3.098 ± 0.2
1.844AsnPhe: 1.844 ± 0.145
3.698AsnGly: 3.698 ± 0.258
0.98AsnHis: 0.98 ± 0.112
2.719AsnIle: 2.719 ± 0.194
2.75AsnLys: 2.75 ± 0.166
3.93AsnLeu: 3.93 ± 0.202
1.307AsnMet: 1.307 ± 0.124
2.286AsnAsn: 2.286 ± 0.15
3.014AsnPro: 3.014 ± 0.173
2.023AsnGln: 2.023 ± 0.163
2.719AsnArg: 2.719 ± 0.162
2.792AsnSer: 2.792 ± 0.151
3.024AsnThr: 3.024 ± 0.165
3.551AsnVal: 3.551 ± 0.187
0.664AsnTrp: 0.664 ± 0.071
1.876AsnTyr: 1.876 ± 0.15
0.0AsnXaa: 0.0 ± 0.0
Pro
3.446ProAla: 3.446 ± 0.182
0.242ProCys: 0.242 ± 0.051
3.172ProAsp: 3.172 ± 0.216
3.372ProGlu: 3.372 ± 0.2
1.718ProPhe: 1.718 ± 0.15
2.624ProGly: 2.624 ± 0.187
1.117ProHis: 1.117 ± 0.113
2.35ProIle: 2.35 ± 0.167
2.35ProLys: 2.35 ± 0.158
3.583ProLeu: 3.583 ± 0.22
1.317ProMet: 1.317 ± 0.134
1.991ProAsn: 1.991 ± 0.148
1.254ProPro: 1.254 ± 0.116
1.739ProGln: 1.739 ± 0.211
1.665ProArg: 1.665 ± 0.146
2.423ProSer: 2.423 ± 0.15
3.151ProThr: 3.151 ± 0.216
3.698ProVal: 3.698 ± 0.22
0.358ProTrp: 0.358 ± 0.065
1.486ProTyr: 1.486 ± 0.136
0.0ProXaa: 0.0 ± 0.0
Gln
3.34GlnAla: 3.34 ± 0.198
0.443GlnCys: 0.443 ± 0.073
1.876GlnAsp: 1.876 ± 0.16
2.381GlnGlu: 2.381 ± 0.163
1.991GlnPhe: 1.991 ± 0.155
2.35GlnGly: 2.35 ± 0.196
1.075GlnHis: 1.075 ± 0.114
2.539GlnIle: 2.539 ± 0.165
2.055GlnLys: 2.055 ± 0.15
4.015GlnLeu: 4.015 ± 0.212
1.212GlnMet: 1.212 ± 0.136
1.675GlnAsn: 1.675 ± 0.142
1.633GlnPro: 1.633 ± 0.192
2.139GlnGln: 2.139 ± 0.334
2.171GlnArg: 2.171 ± 0.155
2.202GlnSer: 2.202 ± 0.168
2.065GlnThr: 2.065 ± 0.159
3.129GlnVal: 3.129 ± 0.195
0.801GlnTrp: 0.801 ± 0.106
1.718GlnTyr: 1.718 ± 0.132
0.0GlnXaa: 0.0 ± 0.0
Arg
3.224ArgAla: 3.224 ± 0.207
0.495ArgCys: 0.495 ± 0.082
2.94ArgAsp: 2.94 ± 0.192
3.266ArgGlu: 3.266 ± 0.209
2.213ArgPhe: 2.213 ± 0.151
2.687ArgGly: 2.687 ± 0.175
1.033ArgHis: 1.033 ± 0.102
3.509ArgIle: 3.509 ± 0.193
3.277ArgLys: 3.277 ± 0.215
5.11ArgLeu: 5.11 ± 0.287
1.865ArgMet: 1.865 ± 0.16
2.381ArgAsn: 2.381 ± 0.143
1.749ArgPro: 1.749 ± 0.159
2.076ArgGln: 2.076 ± 0.14
3.129ArgArg: 3.129 ± 0.214
3.003ArgSer: 3.003 ± 0.168
3.108ArgThr: 3.108 ± 0.198
3.793ArgVal: 3.793 ± 0.211
0.738ArgTrp: 0.738 ± 0.085
2.15ArgTyr: 2.15 ± 0.192
0.0ArgXaa: 0.0 ± 0.0
Ser
4.699SerAla: 4.699 ± 0.328
0.421SerCys: 0.421 ± 0.062
3.33SerAsp: 3.33 ± 0.167
3.372SerGlu: 3.372 ± 0.192
2.434SerPhe: 2.434 ± 0.172
3.783SerGly: 3.783 ± 0.224
1.149SerHis: 1.149 ± 0.121
3.72SerIle: 3.72 ± 0.197
3.235SerLys: 3.235 ± 0.193
5.079SerLeu: 5.079 ± 0.298
1.76SerMet: 1.76 ± 0.133
3.003SerAsn: 3.003 ± 0.195
2.455SerPro: 2.455 ± 0.159
2.308SerGln: 2.308 ± 0.146
2.866SerArg: 2.866 ± 0.176
3.161SerSer: 3.161 ± 0.188
3.888SerThr: 3.888 ± 0.215
4.236SerVal: 4.236 ± 0.197
0.748SerTrp: 0.748 ± 0.092
1.981SerTyr: 1.981 ± 0.133
0.0SerXaa: 0.0 ± 0.0
Thr
4.752ThrAla: 4.752 ± 0.294
0.506ThrCys: 0.506 ± 0.082
4.099ThrAsp: 4.099 ± 0.211
3.804ThrGlu: 3.804 ± 0.207
2.582ThrPhe: 2.582 ± 0.172
4.626ThrGly: 4.626 ± 0.26
1.349ThrHis: 1.349 ± 0.131
4.32ThrIle: 4.32 ± 0.242
3.561ThrLys: 3.561 ± 0.214
5.226ThrLeu: 5.226 ± 0.255
1.517ThrMet: 1.517 ± 0.127
3.024ThrAsn: 3.024 ± 0.186
3.414ThrPro: 3.414 ± 0.201
2.371ThrGln: 2.371 ± 0.168
3.182ThrArg: 3.182 ± 0.182
3.477ThrSer: 3.477 ± 0.195
4.152ThrThr: 4.152 ± 0.249
4.868ThrVal: 4.868 ± 0.231
1.012ThrTrp: 1.012 ± 0.111
2.392ThrTyr: 2.392 ± 0.166
0.0ThrXaa: 0.0 ± 0.0
Val
5.816ValAla: 5.816 ± 0.278
0.717ValCys: 0.717 ± 0.082
4.763ValAsp: 4.763 ± 0.241
4.836ValGlu: 4.836 ± 0.265
2.339ValPhe: 2.339 ± 0.179
4.478ValGly: 4.478 ± 0.266
1.475ValHis: 1.475 ± 0.129
4.721ValIle: 4.721 ± 0.23
4.352ValLys: 4.352 ± 0.249
5.479ValLeu: 5.479 ± 0.231
2.139ValMet: 2.139 ± 0.142
3.646ValAsn: 3.646 ± 0.227
2.866ValPro: 2.866 ± 0.179
2.466ValGln: 2.466 ± 0.185
3.53ValArg: 3.53 ± 0.217
5.068ValSer: 5.068 ± 0.248
5.268ValThr: 5.268 ± 0.231
5.353ValVal: 5.353 ± 0.252
0.801ValTrp: 0.801 ± 0.083
2.655ValTyr: 2.655 ± 0.167
0.0ValXaa: 0.0 ± 0.0
Trp
0.822TrpAla: 0.822 ± 0.088
0.148TrpCys: 0.148 ± 0.039
0.78TrpAsp: 0.78 ± 0.09
0.906TrpGlu: 0.906 ± 0.106
0.832TrpPhe: 0.832 ± 0.102
0.622TrpGly: 0.622 ± 0.084
0.327TrpHis: 0.327 ± 0.071
0.969TrpIle: 0.969 ± 0.106
0.79TrpLys: 0.79 ± 0.086
1.475TrpLeu: 1.475 ± 0.166
0.453TrpMet: 0.453 ± 0.056
0.759TrpAsn: 0.759 ± 0.105
0.4TrpPro: 0.4 ± 0.066
0.537TrpGln: 0.537 ± 0.071
0.727TrpArg: 0.727 ± 0.087
0.685TrpSer: 0.685 ± 0.074
0.822TrpThr: 0.822 ± 0.109
1.264TrpVal: 1.264 ± 0.169
0.084TrpTrp: 0.084 ± 0.031
0.548TrpTyr: 0.548 ± 0.094
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.613TyrAla: 2.613 ± 0.175
0.495TyrCys: 0.495 ± 0.073
2.192TyrAsp: 2.192 ± 0.148
2.065TyrGlu: 2.065 ± 0.18
1.433TyrPhe: 1.433 ± 0.116
2.107TyrGly: 2.107 ± 0.149
0.885TyrHis: 0.885 ± 0.089
2.192TyrIle: 2.192 ± 0.138
1.686TyrLys: 1.686 ± 0.139
3.151TyrLeu: 3.151 ± 0.174
1.054TyrMet: 1.054 ± 0.115
2.034TyrAsn: 2.034 ± 0.148
1.876TyrPro: 1.876 ± 0.145
1.57TyrGln: 1.57 ± 0.143
2.276TyrArg: 2.276 ± 0.186
1.981TyrSer: 1.981 ± 0.16
2.518TyrThr: 2.518 ± 0.174
2.687TyrVal: 2.687 ± 0.205
0.601TyrTrp: 0.601 ± 0.073
1.57TyrTyr: 1.57 ± 0.139
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 420 proteins (94906 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski