Fulvivirga imtechensis AK7
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5952 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|L8JI88|L8JI88_9BACT Uncharacterized protein OS=Fulvivirga imtechensis AK7 OX=1237149 GN=C900_00225 PE=4 SV=1
MM1 pKa = 7.51 GNHH4 pKa = 5.84 LQSLYY9 pKa = 10.81 RR10 pKa = 11.84 HH11 pKa = 6.04 TLTKK15 pKa = 9.29 YY16 pKa = 8.68 KK17 pKa = 10.23 KK18 pKa = 8.44 YY19 pKa = 9.56 RR20 pKa = 11.84 SRR22 pKa = 11.84 YY23 pKa = 7.97 RR24 pKa = 11.84 KK25 pKa = 9.96 SIQTGLYY32 pKa = 8.85 FQYY35 pKa = 10.41 SQKK38 pKa = 10.17 KK39 pKa = 7.71 QRR41 pKa = 11.84 LILEE45 pKa = 4.7 RR46 pKa = 11.84 IEE48 pKa = 4.26 KK49 pKa = 10.13 LKK51 pKa = 10.73 QRR53 pKa = 11.84 LASLKK58 pKa = 9.07 WQIKK62 pKa = 7.43 MAIASGALVMALHH75 pKa = 7.21 AAPAQGQEE83 pKa = 3.93 LGPFVEE89 pKa = 5.89 APAKK93 pKa = 10.8 NPFPPPPVSRR103 pKa = 11.84 LTRR106 pKa = 11.84 EE107 pKa = 3.64 HH108 pKa = 6.77 PAIVDD113 pKa = 3.21 IDD115 pKa = 3.86 NDD117 pKa = 3.41 GDD119 pKa = 4.24 LDD121 pKa = 4.03 VFIGLTSGTIIFLKK135 pKa = 10.87 NEE137 pKa = 3.69 GSAEE141 pKa = 3.83 KK142 pKa = 10.01 PYY144 pKa = 10.51 FVQQFSTDD152 pKa = 3.25 NPANGIDD159 pKa = 3.37 VGYY162 pKa = 10.41 NATPTFADD170 pKa = 3.67 LDD172 pKa = 4.01 GDD174 pKa = 3.89 GDD176 pKa = 4.0 YY177 pKa = 11.62 DD178 pKa = 5.61 LIIGEE183 pKa = 4.24 YY184 pKa = 9.82 DD185 pKa = 3.16 QYY187 pKa = 11.54 PYY189 pKa = 10.61 TPYY192 pKa = 11.61 ANINYY197 pKa = 9.55 YY198 pKa = 10.52 EE199 pKa = 4.15 NTGTASAPVFTLPSGEE215 pKa = 4.36 HH216 pKa = 6.07 PMDD219 pKa = 3.58 NVFSDD224 pKa = 3.52 SFYY227 pKa = 10.57 GHH229 pKa = 7.4 PAFVDD234 pKa = 3.03 IDD236 pKa = 3.82 NDD238 pKa = 3.4 GDD240 pKa = 4.22 LDD242 pKa = 4.13 VFVGGARR249 pKa = 11.84 NTIYY253 pKa = 9.83 ATYY256 pKa = 10.66 RR257 pKa = 11.84 EE258 pKa = 4.53 TIKK261 pKa = 10.47 FWRR264 pKa = 11.84 NDD266 pKa = 2.57 GSLPVAQFTAEE277 pKa = 3.96 EE278 pKa = 4.57 GASNLISIVNPEE290 pKa = 3.94 FGGTLDD296 pKa = 3.27 HH297 pKa = 6.99 AAPVLVDD304 pKa = 3.56 IDD306 pKa = 4.07 GDD308 pKa = 3.76 GDD310 pKa = 3.44 KK311 pKa = 11.62 DD312 pKa = 3.77 MFVGDD317 pKa = 3.7 INGDD321 pKa = 2.76 IRR323 pKa = 11.84 FFRR326 pKa = 11.84 NDD328 pKa = 2.79 GTAEE332 pKa = 4.18 SPSFPAEE339 pKa = 3.6 ITGTGNPFDD348 pKa = 4.9 GLTAPSFASPEE359 pKa = 4.03 FGDD362 pKa = 4.22 FDD364 pKa = 4.81 NDD366 pKa = 3.21 GDD368 pKa = 3.72 FDD370 pKa = 6.23 AIVGGGSSDD379 pKa = 3.66 HH380 pKa = 5.98 IQYY383 pKa = 10.49 FEE385 pKa = 4.74 NIGTASAPQFVEE397 pKa = 4.12 RR398 pKa = 11.84 FGPLNPFQGVDD409 pKa = 3.33 VGYY412 pKa = 10.09 HH413 pKa = 4.63 STPAFVDD420 pKa = 2.98 IDD422 pKa = 3.79 NDD424 pKa = 3.59 GDD426 pKa = 3.73 LDD428 pKa = 3.89 AFIGGKK434 pKa = 9.88 YY435 pKa = 9.86 SGYY438 pKa = 8.06 NTGFVRR444 pKa = 11.84 FFEE447 pKa = 4.37 NTNGTFQEE455 pKa = 4.49 KK456 pKa = 10.61 NSTEE460 pKa = 4.03 NPVEE464 pKa = 4.06 GLSNVNFQNPSFVDD478 pKa = 3.03 IDD480 pKa = 3.95 GDD482 pKa = 3.94 DD483 pKa = 4.04 DD484 pKa = 3.75 QDD486 pKa = 3.74 YY487 pKa = 10.74 FLDD490 pKa = 3.6 IDD492 pKa = 4.17 YY493 pKa = 10.89 DD494 pKa = 3.52 ILFYY498 pKa = 11.25 EE499 pKa = 4.3 NTGNASSMEE508 pKa = 4.18 LGSANSSLLTPDD520 pKa = 4.3 LEE522 pKa = 4.93 GIVYY526 pKa = 10.26 NYY528 pKa = 7.55 KK529 pKa = 8.33 TTFADD534 pKa = 3.11 VDD536 pKa = 3.74 RR537 pKa = 11.84 DD538 pKa = 3.52 GDD540 pKa = 3.72 LDD542 pKa = 5.11 AIGAHH547 pKa = 5.64 TGYY550 pKa = 10.01 PGGNLKK556 pKa = 10.52 YY557 pKa = 10.11 YY558 pKa = 10.8 KK559 pKa = 9.5 NTGTVNSPVFTLDD572 pKa = 3.57 DD573 pKa = 4.46 LNNPFSTLTEE583 pKa = 4.07 TDD585 pKa = 3.4 LSYY588 pKa = 11.4 SPTPEE593 pKa = 4.38 FVDD596 pKa = 3.34 IDD598 pKa = 4.39 HH599 pKa = 7.04 NGHH602 pKa = 6.8 ADD604 pKa = 3.62 LFVGRR609 pKa = 11.84 NTGTILFFPNTGAEE623 pKa = 4.14 SFDD626 pKa = 3.78 ISMTSANNPLAAVNVSSDD644 pKa = 3.45 AAPAFADD651 pKa = 3.27 IDD653 pKa = 4.08 GDD655 pKa = 3.93 GDD657 pKa = 3.82 LDD659 pKa = 3.88 AFIGSFSGRR668 pKa = 11.84 ITYY671 pKa = 9.59 FEE673 pKa = 4.41 NQNQPPEE680 pKa = 3.85 IVTNVGSALSFTEE693 pKa = 3.7 NDD695 pKa = 3.28 APLILDD701 pKa = 3.79 AGFAISDD708 pKa = 4.64 DD709 pKa = 3.95 GDD711 pKa = 4.11 DD712 pKa = 5.07 DD713 pKa = 4.84 IISAEE718 pKa = 4.09 VAITANYY725 pKa = 9.59 INGEE729 pKa = 4.22 DD730 pKa = 3.73 VLTFTPQGGVTGSFNASTGVLSLSGYY756 pKa = 10.75 AKK758 pKa = 10.15 LAVYY762 pKa = 9.79 EE763 pKa = 4.72 LVLQSITYY771 pKa = 9.74 EE772 pKa = 4.09 NTSEE776 pKa = 4.2 NPSPAPRR783 pKa = 11.84 TIEE786 pKa = 3.64 LTAIDD791 pKa = 5.04 FDD793 pKa = 4.1 ATNPTSATSMTVNVIPVNDD812 pKa = 4.15 APVLTATGSNVTYY825 pKa = 10.57 TEE827 pKa = 4.58 DD828 pKa = 4.46 DD829 pKa = 3.89 DD830 pKa = 5.18 PGVVIDD836 pKa = 4.53 PGVIISDD843 pKa = 3.57 VDD845 pKa = 3.34 NTTLSGATIAITANFVDD862 pKa = 4.39 TEE864 pKa = 4.31 DD865 pKa = 3.88 VLDD868 pKa = 3.99 FTDD871 pKa = 3.69 QNGITGNYY879 pKa = 8.69 DD880 pKa = 2.94 AATGVLTLSGSATLAQYY897 pKa = 9.1 EE898 pKa = 4.03 AALRR902 pKa = 11.84 SIAYY906 pKa = 9.87 RR907 pKa = 11.84 NTSQNPDD914 pKa = 3.32 PATRR918 pKa = 11.84 TVTFEE923 pKa = 4.31 VNDD926 pKa = 3.88 GTDD929 pKa = 3.35 PSNTGTATVAVVPVNDD945 pKa = 4.05 APVISSNNGTTATDD959 pKa = 4.17 YY960 pKa = 10.96 YY961 pKa = 11.11 VEE963 pKa = 4.32 EE964 pKa = 4.57 SPVIIDD970 pKa = 3.61 NLITISDD977 pKa = 3.71 VDD979 pKa = 3.98 DD980 pKa = 4.03 TDD982 pKa = 4.93 LEE984 pKa = 4.84 AGEE987 pKa = 4.27 VRR989 pKa = 11.84 ITDD992 pKa = 4.16 FVAGDD997 pKa = 3.58 VLHH1000 pKa = 6.58 FTDD1003 pKa = 3.82 QNGITGSYY1011 pKa = 8.04 DD1012 pKa = 3.3 TQTGILALSGTATIEE1027 pKa = 4.0 NYY1029 pKa = 7.34 TTALQSITFEE1039 pKa = 4.41 NNNAASGQRR1048 pKa = 11.84 QIEE1051 pKa = 4.44 FIINDD1056 pKa = 3.73 GNADD1060 pKa = 3.52 SSPYY1064 pKa = 8.7 IRR1066 pKa = 11.84 NMNVIANEE1074 pKa = 4.06 PPVVDD1079 pKa = 3.93 TTPQSTQVGNIVTIDD1094 pKa = 3.72 LCDD1097 pKa = 5.38 LISDD1101 pKa = 4.33 PDD1103 pKa = 3.54 NTYY1106 pKa = 10.92 SEE1108 pKa = 4.27 LTITVVSITSGAATDD1123 pKa = 3.65 ISDD1126 pKa = 5.13 CILTINYY1133 pKa = 8.94 GSTGFSGQDD1142 pKa = 3.13 QLVLQACDD1150 pKa = 3.43 PSGACDD1156 pKa = 3.19 QNTINIQVDD1165 pKa = 4.38 EE1166 pKa = 4.47 PSGPLTIYY1174 pKa = 10.77 NAVSPNGDD1182 pKa = 3.61 GLNDD1186 pKa = 2.57 WWEE1189 pKa = 4.29 IVNLTSPNKK1198 pKa = 8.95 IALYY1202 pKa = 10.11 NRR1204 pKa = 11.84 WGDD1207 pKa = 3.73 LVKK1210 pKa = 10.1 TLTDD1214 pKa = 3.97 YY1215 pKa = 11.42 AGHH1218 pKa = 6.42 TPNTLLNDD1226 pKa = 4.36 LPAGTYY1232 pKa = 9.11 YY1233 pKa = 11.12 YY1234 pKa = 10.36 KK1235 pKa = 10.42 IKK1237 pKa = 10.63 SPQGDD1242 pKa = 3.81 YY1243 pKa = 11.14 EE1244 pKa = 4.8 GYY1246 pKa = 10.82 LVIKK1250 pKa = 10.16 KK1251 pKa = 10.14
Molecular weight: 134.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.673
IPC2_protein 3.732
IPC_protein 3.795
Toseland 3.554
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.732
Rodwell 3.617
Grimsley 3.465
Solomon 3.795
Lehninger 3.745
Nozaki 3.897
DTASelect 4.19
Thurlkill 3.605
EMBOSS 3.745
Sillero 3.923
Patrickios 1.214
IPC_peptide 3.783
IPC2_peptide 3.897
IPC2.peptide.svr19 3.806
Protein with the highest isoelectric point:
>tr|L8JSF3|L8JSF3_9BACT Uncharacterized protein OS=Fulvivirga imtechensis AK7 OX=1237149 GN=C900_02145 PE=4 SV=1
MM1 pKa = 7.08 QVDD4 pKa = 3.5 IFDD7 pKa = 5.93 AILSDD12 pKa = 3.91 SVYY15 pKa = 10.94 LEE17 pKa = 4.06 KK18 pKa = 10.55 FLNQMHH24 pKa = 6.47 SKK26 pKa = 8.91 YY27 pKa = 10.39 PPMHH31 pKa = 6.81 AMRR34 pKa = 11.84 ARR36 pKa = 11.84 MMRR39 pKa = 11.84 RR40 pKa = 11.84 MYY42 pKa = 10.42 RR43 pKa = 11.84 FSEE46 pKa = 4.04 VDD48 pKa = 3.34 SLVIADD54 pKa = 3.63 TLIRR58 pKa = 11.84 RR59 pKa = 11.84 NMLKK63 pKa = 10.79 LMLNQADD70 pKa = 4.12 RR71 pKa = 11.84 DD72 pKa = 4.46 SVFCRR77 pKa = 11.84 QMSQSIMKK85 pKa = 9.65 HH86 pKa = 4.68 RR87 pKa = 11.84 PLRR90 pKa = 11.84 KK91 pKa = 9.16 RR92 pKa = 11.84 LRR94 pKa = 11.84 QHH96 pKa = 5.85 MGVPEE101 pKa = 3.87
Molecular weight: 12.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.332
IPC2_protein 9.604
IPC_protein 10.409
Toseland 10.57
ProMoST 10.277
Dawson 10.687
Bjellqvist 10.394
Wikipedia 10.891
Rodwell 10.877
Grimsley 10.745
Solomon 10.789
Lehninger 10.76
Nozaki 10.555
DTASelect 10.394
Thurlkill 10.584
EMBOSS 10.979
Sillero 10.613
Patrickios 10.628
IPC_peptide 10.804
IPC2_peptide 9.341
IPC2.peptide.svr19 8.635
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5952
0
5952
1920940
37
7274
322.7
36.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.857 ± 0.029
0.762 ± 0.011
5.665 ± 0.023
6.783 ± 0.03
4.835 ± 0.029
6.855 ± 0.034
2.0 ± 0.016
7.336 ± 0.031
6.728 ± 0.045
9.409 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.282 ± 0.016
5.3 ± 0.029
3.625 ± 0.024
3.543 ± 0.02
4.211 ± 0.022
6.496 ± 0.031
5.45 ± 0.042
6.536 ± 0.027
1.134 ± 0.011
4.192 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here