Anaerostipes sp. CAG:276

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Anaerostipes; environmental samples

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3141 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6QC27|R6QC27_9FIRM Oxidoreductase OS=Anaerostipes sp. CAG:276 OX=1262699 GN=BN583_02625 PE=4 SV=1
MM1 pKa = 7.33GHH3 pKa = 7.09RR4 pKa = 11.84SDD6 pKa = 4.75LLDD9 pKa = 3.74YY10 pKa = 11.39LEE12 pKa = 5.64LGLAVQVDD20 pKa = 5.54GIPVDD25 pKa = 3.65NSNQEE30 pKa = 3.83QMDD33 pKa = 4.01LMLEE37 pKa = 4.13EE38 pKa = 5.85DD39 pKa = 4.04DD40 pKa = 6.19SYY42 pKa = 11.72MKK44 pKa = 10.84DD45 pKa = 3.83FIDD48 pKa = 4.33DD49 pKa = 3.81EE50 pKa = 5.09KK51 pKa = 11.91GEE53 pKa = 4.13ICAVYY58 pKa = 8.34YY59 pKa = 10.34QKK61 pKa = 9.54VTNHH65 pKa = 5.7

Molecular weight: 7.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6QK20|R6QK20_9FIRM DUF305 domain-containing protein OS=Anaerostipes sp. CAG:276 OX=1262699 GN=BN583_01217 PE=4 SV=1
MM1 pKa = 7.74ILPPFWVYY9 pKa = 7.63FTAFVIKK16 pKa = 10.55LISTCLILPASPIKK30 pKa = 10.68SVWTTSTVLNKK41 pKa = 10.41SSWPFCSASGRR52 pKa = 11.84VIEE55 pKa = 5.11LISVISSLRR64 pKa = 11.84QNGSSVSSIFPFSIFDD80 pKa = 3.36ISRR83 pKa = 11.84ISLIRR88 pKa = 11.84SSRR91 pKa = 11.84WLLDD95 pKa = 2.82MSIFVRR101 pKa = 11.84HH102 pKa = 6.06SLTFSGSLILDD113 pKa = 3.72AAMEE117 pKa = 4.46VMPMIAFIGVLISWDD132 pKa = 2.39IWARR136 pKa = 11.84KK137 pKa = 9.31LLFASLAFLAASYY150 pKa = 9.92AASSAFLFLSSCSFSSSTLWAARR173 pKa = 11.84MTFTGIPFRR182 pKa = 11.84LSANMIWMRR191 pKa = 11.84IHH193 pKa = 6.84PP194 pKa = 4.45

Molecular weight: 21.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3141

0

3141

938375

29

5626

298.8

33.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.2 ± 0.046

1.462 ± 0.019

5.541 ± 0.041

7.645 ± 0.046

4.214 ± 0.029

7.467 ± 0.042

1.785 ± 0.019

7.411 ± 0.041

7.691 ± 0.042

8.936 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.153 ± 0.027

3.97 ± 0.033

3.29 ± 0.025

3.167 ± 0.025

4.415 ± 0.034

5.888 ± 0.037

5.232 ± 0.035

6.908 ± 0.038

0.769 ± 0.013

3.853 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski