Anaerostipes sp. CAG:276
Average proteome isoelectric point is 6.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3141 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R6QC27|R6QC27_9FIRM Oxidoreductase OS=Anaerostipes sp. CAG:276 OX=1262699 GN=BN583_02625 PE=4 SV=1
MM1 pKa = 7.33 GHH3 pKa = 7.09 RR4 pKa = 11.84 SDD6 pKa = 4.75 LLDD9 pKa = 3.74 YY10 pKa = 11.39 LEE12 pKa = 5.64 LGLAVQVDD20 pKa = 5.54 GIPVDD25 pKa = 3.65 NSNQEE30 pKa = 3.83 QMDD33 pKa = 4.01 LMLEE37 pKa = 4.13 EE38 pKa = 5.85 DD39 pKa = 4.04 DD40 pKa = 6.19 SYY42 pKa = 11.72 MKK44 pKa = 10.84 DD45 pKa = 3.83 FIDD48 pKa = 4.33 DD49 pKa = 3.81 EE50 pKa = 5.09 KK51 pKa = 11.91 GEE53 pKa = 4.13 ICAVYY58 pKa = 8.34 YY59 pKa = 10.34 QKK61 pKa = 9.54 VTNHH65 pKa = 5.7
Molecular weight: 7.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.75
IPC2_protein 3.897
IPC_protein 3.834
Toseland 3.63
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.999
Wikipedia 3.783
Rodwell 3.668
Grimsley 3.541
Solomon 3.821
Lehninger 3.77
Nozaki 3.961
DTASelect 4.19
Thurlkill 3.694
EMBOSS 3.795
Sillero 3.961
Patrickios 1.901
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.866
Protein with the highest isoelectric point:
>tr|R6QK20|R6QK20_9FIRM DUF305 domain-containing protein OS=Anaerostipes sp. CAG:276 OX=1262699 GN=BN583_01217 PE=4 SV=1
MM1 pKa = 7.74 ILPPFWVYY9 pKa = 7.63 FTAFVIKK16 pKa = 10.55 LISTCLILPASPIKK30 pKa = 10.68 SVWTTSTVLNKK41 pKa = 10.41 SSWPFCSASGRR52 pKa = 11.84 VIEE55 pKa = 5.11 LISVISSLRR64 pKa = 11.84 QNGSSVSSIFPFSIFDD80 pKa = 3.36 ISRR83 pKa = 11.84 ISLIRR88 pKa = 11.84 SSRR91 pKa = 11.84 WLLDD95 pKa = 2.82 MSIFVRR101 pKa = 11.84 HH102 pKa = 6.06 SLTFSGSLILDD113 pKa = 3.72 AAMEE117 pKa = 4.46 VMPMIAFIGVLISWDD132 pKa = 2.39 IWARR136 pKa = 11.84 KK137 pKa = 9.31 LLFASLAFLAASYY150 pKa = 9.92 AASSAFLFLSSCSFSSSTLWAARR173 pKa = 11.84 MTFTGIPFRR182 pKa = 11.84 LSANMIWMRR191 pKa = 11.84 IHH193 pKa = 6.84 PP194 pKa = 4.45
Molecular weight: 21.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.341
IPC2_protein 9.487
IPC_protein 10.072
Toseland 10.452
ProMoST 10.116
Dawson 10.57
Bjellqvist 10.292
Wikipedia 10.76
Rodwell 10.804
Grimsley 10.628
Solomon 10.672
Lehninger 10.643
Nozaki 10.496
DTASelect 10.262
Thurlkill 10.467
EMBOSS 10.847
Sillero 10.511
Patrickios 10.599
IPC_peptide 10.672
IPC2_peptide 9.545
IPC2.peptide.svr19 8.456
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3141
0
3141
938375
29
5626
298.8
33.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.2 ± 0.046
1.462 ± 0.019
5.541 ± 0.041
7.645 ± 0.046
4.214 ± 0.029
7.467 ± 0.042
1.785 ± 0.019
7.411 ± 0.041
7.691 ± 0.042
8.936 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.153 ± 0.027
3.97 ± 0.033
3.29 ± 0.025
3.167 ± 0.025
4.415 ± 0.034
5.888 ± 0.037
5.232 ± 0.035
6.908 ± 0.038
0.769 ± 0.013
3.853 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here