Cyanophage P-TIM40

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Libanvirus; Prochlorococcus virus PTIM40

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 235 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5ADW5|A0A0C5ADW5_9CAUD Uncharacterized protein OS=Cyanophage P-TIM40 OX=1589733 GN=PTIM40_75 PE=4 SV=1
MM1 pKa = 7.84NDD3 pKa = 3.16NQINWLSDD11 pKa = 2.91IYY13 pKa = 11.15VQYY16 pKa = 9.92CTDD19 pKa = 3.67HH20 pKa = 6.41NLPMLSADD28 pKa = 3.95EE29 pKa = 4.12QDD31 pKa = 3.85LEE33 pKa = 4.6SMSVANRR40 pKa = 11.84DD41 pKa = 3.32WITTFISIWEE51 pKa = 4.2GLSS54 pKa = 3.23

Molecular weight: 6.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5AB17|A0A0C5AB17_9CAUD Uncharacterized protein OS=Cyanophage P-TIM40 OX=1589733 GN=PTIM40_149 PE=4 SV=1
MM1 pKa = 7.04TKK3 pKa = 9.98KK4 pKa = 10.5DD5 pKa = 3.44RR6 pKa = 11.84GNVPSAYY13 pKa = 10.07AYY15 pKa = 7.41TCKK18 pKa = 10.23EE19 pKa = 3.78CTIRR23 pKa = 11.84RR24 pKa = 11.84VNKK27 pKa = 8.82SRR29 pKa = 11.84KK30 pKa = 9.27RR31 pKa = 11.84RR32 pKa = 11.84VKK34 pKa = 10.05WSDD37 pKa = 3.35YY38 pKa = 10.72PDD40 pKa = 2.96WW41 pKa = 5.89

Molecular weight: 4.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

235

0

235

61416

23

7351

261.3

29.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.78 ± 0.207

0.972 ± 0.099

6.896 ± 0.188

5.849 ± 0.219

4.29 ± 0.129

7.851 ± 0.313

1.802 ± 0.145

6.379 ± 0.255

6.158 ± 0.467

6.957 ± 0.208

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.151 ± 0.221

5.761 ± 0.224

4.173 ± 0.17

3.649 ± 0.093

4.132 ± 0.142

6.801 ± 0.171

7.278 ± 0.447

6.49 ± 0.198

1.247 ± 0.091

4.382 ± 0.173
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski