Almpiwar virus
Average proteome isoelectric point is 7.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A024A105|A0A024A105_9RHAB Uncharacterized protein OS=Almpiwar virus OX=318843 GN=U1 PE=4 SV=1
MM1 pKa = 7.45 SFNDD5 pKa = 3.9 FSLTFSPIALKK16 pKa = 10.24 AAKK19 pKa = 10.09 DD20 pKa = 3.39 HH21 pKa = 7.51 DD22 pKa = 3.95 EE23 pKa = 5.09 AEE25 pKa = 4.39 INMGTKK31 pKa = 9.9 FDD33 pKa = 4.2 LEE35 pKa = 4.2 NRR37 pKa = 11.84 SNQSPTQAFSSSTSEE52 pKa = 3.87 EE53 pKa = 3.95 VSVEE57 pKa = 4.02 KK58 pKa = 9.49 TQSSQSVHH66 pKa = 5.24 GVEE69 pKa = 4.69 FKK71 pKa = 10.5 IPRR74 pKa = 11.84 VGIPATQQEE83 pKa = 4.6 LDD85 pKa = 4.49 DD86 pKa = 5.32 IIGSVIDD93 pKa = 5.59 LVLAQNKK100 pKa = 9.23 IEE102 pKa = 4.06 HH103 pKa = 6.9 HH104 pKa = 7.16 DD105 pKa = 3.63 IEE107 pKa = 4.68 ITDD110 pKa = 3.44 KK111 pKa = 10.97 AYY113 pKa = 9.71 TYY115 pKa = 8.36 FIPTSKK121 pKa = 10.13 RR122 pKa = 11.84 AKK124 pKa = 10.4 GYY126 pKa = 7.69 DD127 pKa = 3.25 TSEE130 pKa = 3.86 EE131 pKa = 4.34 TFDD134 pKa = 4.31 SKK136 pKa = 10.23 TPANSEE142 pKa = 3.8 EE143 pKa = 4.49 DD144 pKa = 3.73 EE145 pKa = 4.52 EE146 pKa = 6.27 ADD148 pKa = 4.9 EE149 pKa = 6.07 DD150 pKa = 4.15 DD151 pKa = 5.05 QEE153 pKa = 5.05 SDD155 pKa = 3.65 SPGSSEE161 pKa = 4.99 EE162 pKa = 3.98 IDD164 pKa = 3.57 SEE166 pKa = 4.79 GPSDD170 pKa = 3.53 EE171 pKa = 5.87 DD172 pKa = 4.02 EE173 pKa = 5.16 IEE175 pKa = 4.17 EE176 pKa = 4.94 QEE178 pKa = 4.28 GEE180 pKa = 4.22 PIKK183 pKa = 11.34 GNLEE187 pKa = 3.82 KK188 pKa = 10.75 LQKK191 pKa = 10.65 EE192 pKa = 4.02 KK193 pKa = 10.93 FGNKK197 pKa = 8.49 KK198 pKa = 8.35 QAEE201 pKa = 4.28 RR202 pKa = 11.84 LWRR205 pKa = 11.84 SLNEE209 pKa = 4.62 GIEE212 pKa = 4.31 VPLITGDD219 pKa = 3.34 IALVNQDD226 pKa = 3.39 NLGITKK232 pKa = 10.19 QMLKK236 pKa = 10.43 PLDD239 pKa = 4.99 FEE241 pKa = 6.31 DD242 pKa = 4.35 DD243 pKa = 3.47 WTPVYY248 pKa = 10.17 CLKK251 pKa = 10.82 YY252 pKa = 9.25 VAFRR256 pKa = 11.84 YY257 pKa = 10.17 SEE259 pKa = 4.16 VCAAGCLIDD268 pKa = 4.08 WSDD271 pKa = 3.07 ITKK274 pKa = 10.57 SS275 pKa = 3.26
Molecular weight: 30.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.386
IPC2_protein 4.215
IPC_protein 4.177
Toseland 3.999
ProMoST 4.291
Dawson 4.126
Bjellqvist 4.279
Wikipedia 3.999
Rodwell 4.012
Grimsley 3.91
Solomon 4.126
Lehninger 4.075
Nozaki 4.24
DTASelect 4.406
Thurlkill 4.012
EMBOSS 4.024
Sillero 4.291
Patrickios 3.821
IPC_peptide 4.126
IPC2_peptide 4.279
IPC2.peptide.svr19 4.209
Protein with the highest isoelectric point:
>tr|A0A024A267|A0A024A267_9RHAB Isoform of A0A024A0M0 Protein C OS=Almpiwar virus OX=318843 GN=P PE=4 SV=1
MM1 pKa = 7.23 TSLVQSLTWFWLRR14 pKa = 11.84 IRR16 pKa = 11.84 LNIMILRR23 pKa = 11.84 SRR25 pKa = 11.84 IKK27 pKa = 9.34 HH28 pKa = 5.41 TPTSSQHH35 pKa = 6.28 LKK37 pKa = 8.13 EE38 pKa = 4.31 QKK40 pKa = 10.98 DD41 pKa = 3.47 MTQVRR46 pKa = 11.84 RR47 pKa = 11.84 LLTQRR52 pKa = 11.84 HH53 pKa = 4.71 QLTLKK58 pKa = 10.53 RR59 pKa = 11.84 MKK61 pKa = 10.28 KK62 pKa = 7.23 QMKK65 pKa = 8.69 MIRR68 pKa = 11.84 RR69 pKa = 11.84 VILLDD74 pKa = 3.69 PLKK77 pKa = 10.87 KK78 pKa = 10.65 LILKK82 pKa = 9.05 VRR84 pKa = 11.84 LMKK87 pKa = 10.27 TRR89 pKa = 11.84 LKK91 pKa = 10.47 NKK93 pKa = 9.4 RR94 pKa = 11.84 VNPSRR99 pKa = 11.84 AILRR103 pKa = 11.84 NCRR106 pKa = 11.84 RR107 pKa = 11.84 KK108 pKa = 10.19 NSEE111 pKa = 3.51 TRR113 pKa = 11.84 SRR115 pKa = 11.84 LKK117 pKa = 10.92 DD118 pKa = 3.4 CGEE121 pKa = 3.86 VLMRR125 pKa = 11.84 EE126 pKa = 4.33
Molecular weight: 15.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.187
IPC2_protein 10.321
IPC_protein 11.842
Toseland 12.032
ProMoST 12.501
Dawson 12.032
Bjellqvist 12.018
Wikipedia 12.501
Rodwell 11.901
Grimsley 12.076
Solomon 12.515
Lehninger 12.413
Nozaki 12.032
DTASelect 12.018
Thurlkill 12.032
EMBOSS 12.53
Sillero 12.032
Patrickios 11.608
IPC_peptide 12.515
IPC2_peptide 11.491
IPC2.peptide.svr19 9.258
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
2
8
3823
79
2095
477.9
54.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.107 ± 0.806
2.066 ± 0.241
5.336 ± 0.522
6.016 ± 0.77
4.813 ± 0.342
5.938 ± 0.447
2.433 ± 0.154
7.743 ± 1.026
7.167 ± 0.363
9.547 ± 0.892
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.668 ± 0.252
5.493 ± 0.538
4.028 ± 0.283
3.139 ± 0.293
4.63 ± 0.622
8.057 ± 0.324
6.121 ± 0.371
4.839 ± 0.334
1.962 ± 0.175
3.897 ± 0.402
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here