Clostridium cadaveris
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3342 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I2L082|A0A1I2L082_9CLOT GntR family transcriptional regulator OS=Clostridium cadaveris OX=1529 GN=DBY38_14805 PE=4 SV=1
MM1 pKa = 6.53 VAAVATCVLLVGGSTVAYY19 pKa = 9.84 AADD22 pKa = 3.53 LGGIQRR28 pKa = 11.84 IVQLWIHH35 pKa = 5.95 GDD37 pKa = 3.3 KK38 pKa = 10.93 TEE40 pKa = 4.03 VSIDD44 pKa = 3.68 FNSDD48 pKa = 2.22 GSYY51 pKa = 11.63 DD52 pKa = 3.44 MEE54 pKa = 5.22 YY55 pKa = 10.94 YY56 pKa = 10.67 DD57 pKa = 4.63 NKK59 pKa = 11.0 GNVIQEE65 pKa = 4.28 SGGGVASNLNGSEE78 pKa = 4.04 RR79 pKa = 11.84 PITEE83 pKa = 4.57 DD84 pKa = 3.25 EE85 pKa = 4.85 LMDD88 pKa = 4.66 HH89 pKa = 7.15 LDD91 pKa = 4.0 DD92 pKa = 5.09 PEE94 pKa = 3.94 VRR96 pKa = 11.84 YY97 pKa = 8.9 EE98 pKa = 3.83
Molecular weight: 10.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.773
IPC2_protein 3.961
IPC_protein 3.897
Toseland 3.706
ProMoST 4.024
Dawson 3.884
Bjellqvist 4.101
Wikipedia 3.821
Rodwell 3.732
Grimsley 3.617
Solomon 3.872
Lehninger 3.834
Nozaki 4.012
DTASelect 4.215
Thurlkill 3.757
EMBOSS 3.834
Sillero 4.024
Patrickios 1.914
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.931
Protein with the highest isoelectric point:
>tr|A0A1I2JIU3|A0A1I2JIU3_9CLOT P-type Ca(2+) transporter OS=Clostridium cadaveris OX=1529 GN=DBY38_13050 PE=4 SV=1
MM1 pKa = 7.33 SRR3 pKa = 11.84 KK4 pKa = 9.54 CEE6 pKa = 3.93 YY7 pKa = 9.77 CDD9 pKa = 3.38 KK10 pKa = 11.16 GVISGVQYY18 pKa = 10.79 SHH20 pKa = 6.38 SHH22 pKa = 5.36 RR23 pKa = 11.84 QSKK26 pKa = 10.2 RR27 pKa = 11.84 KK28 pKa = 7.58 WAPNIKK34 pKa = 9.44 RR35 pKa = 11.84 VKK37 pKa = 10.27 AIVNGTPKK45 pKa = 9.69 TVHH48 pKa = 4.9 VCTRR52 pKa = 11.84 CLRR55 pKa = 11.84 SGKK58 pKa = 7.37 VQRR61 pKa = 11.84 AII63 pKa = 3.7
Molecular weight: 7.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.324
IPC2_protein 9.78
IPC_protein 10.233
Toseland 10.833
ProMoST 10.409
Dawson 10.906
Bjellqvist 10.57
Wikipedia 11.067
Rodwell 11.33
Grimsley 10.935
Solomon 10.994
Lehninger 10.979
Nozaki 10.833
DTASelect 10.555
Thurlkill 10.818
EMBOSS 11.228
Sillero 10.833
Patrickios 11.096
IPC_peptide 11.008
IPC2_peptide 9.706
IPC2.peptide.svr19 8.528
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3342
0
3342
1031823
26
2909
308.7
34.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.821 ± 0.046
1.243 ± 0.019
5.66 ± 0.035
7.491 ± 0.053
4.363 ± 0.034
6.602 ± 0.039
1.381 ± 0.017
9.711 ± 0.047
8.939 ± 0.043
8.963 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.829 ± 0.02
5.997 ± 0.041
2.834 ± 0.021
2.195 ± 0.02
3.404 ± 0.028
6.362 ± 0.035
4.937 ± 0.033
6.512 ± 0.036
0.667 ± 0.013
4.091 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here