Consotaella salsifontis
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4266 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1T4P999|A0A1T4P999_9RHIZ Uncharacterized protein OS=Consotaella salsifontis OX=1365950 GN=SAMN05428963_103391 PE=4 SV=1
MM1 pKa = 7.47 PVITGDD7 pKa = 3.25 SGNNEE12 pKa = 4.21 LVGTEE17 pKa = 3.97 ADD19 pKa = 4.06 DD20 pKa = 4.79 VISGLDD26 pKa = 3.54 GDD28 pKa = 5.51 DD29 pKa = 4.53 DD30 pKa = 5.21 LSGLGGSDD38 pKa = 3.33 TLDD41 pKa = 3.57 GGDD44 pKa = 4.12 GYY46 pKa = 11.5 DD47 pKa = 3.48 SIRR50 pKa = 11.84 YY51 pKa = 8.48 VGNSQGVVVTFTGQGSGTVEE71 pKa = 3.91 EE72 pKa = 4.51 NAIDD76 pKa = 3.72 TDD78 pKa = 4.02 SFTGIEE84 pKa = 4.32 SVVGSQYY91 pKa = 11.6 GDD93 pKa = 3.19 VFTNSSSGYY102 pKa = 9.98 ARR104 pKa = 11.84 FRR106 pKa = 11.84 GLDD109 pKa = 3.95 GIDD112 pKa = 3.48 TYY114 pKa = 11.59 NGSEE118 pKa = 4.04 TGFDD122 pKa = 3.52 FLDD125 pKa = 4.13 FLRR128 pKa = 11.84 DD129 pKa = 3.47 ANFGGTGGVTVDD141 pKa = 4.31 LRR143 pKa = 11.84 SGTAIDD149 pKa = 3.88 GFGNLEE155 pKa = 4.01 SLSYY159 pKa = 10.62 IDD161 pKa = 3.81 GARR164 pKa = 11.84 GTEE167 pKa = 3.71 QDD169 pKa = 4.35 DD170 pKa = 4.16 FFIGDD175 pKa = 3.93 DD176 pKa = 3.84 GDD178 pKa = 3.61 NQFRR182 pKa = 11.84 GEE184 pKa = 4.23 GGNDD188 pKa = 3.3 DD189 pKa = 3.62 MRR191 pKa = 11.84 ATGGSDD197 pKa = 3.33 SFDD200 pKa = 3.31 GGAGSDD206 pKa = 3.58 SIQYY210 pKa = 10.79 NDD212 pKa = 3.04 ATQAIVAHH220 pKa = 5.53 FTGDD224 pKa = 3.01 LSGYY228 pKa = 7.81 VLEE231 pKa = 5.12 GSEE234 pKa = 4.24 FTDD237 pKa = 3.07 TFVDD241 pKa = 3.93 VEE243 pKa = 4.61 AVFGTAFGDD252 pKa = 3.76 TFVNEE257 pKa = 4.26 SSSFARR263 pKa = 11.84 FRR265 pKa = 11.84 GFDD268 pKa = 3.44 GVDD271 pKa = 3.24 SYY273 pKa = 11.55 TGSASGFDD281 pKa = 3.78 MIDD284 pKa = 3.93 FYY286 pKa = 11.65 RR287 pKa = 11.84 DD288 pKa = 2.8 ARR290 pKa = 11.84 YY291 pKa = 10.24 GGTSGVTVDD300 pKa = 4.43 LAAGTGTDD308 pKa = 2.98 GFGNVEE314 pKa = 4.28 SFSNIDD320 pKa = 3.38 GVRR323 pKa = 11.84 GTDD326 pKa = 3.75 FNDD329 pKa = 3.09 VLTGSAGANTLDD341 pKa = 3.88 GAGGADD347 pKa = 4.57 LMRR350 pKa = 11.84 GLAGDD355 pKa = 4.04 DD356 pKa = 3.54 VLVGSGADD364 pKa = 3.94 GDD366 pKa = 4.21 TADD369 pKa = 3.71 YY370 pKa = 11.12 SLDD373 pKa = 3.47 VLYY376 pKa = 10.76 GATHH380 pKa = 7.52 GIRR383 pKa = 11.84 VNQLGNGNQGGLPPDD398 pKa = 3.71 TVIDD402 pKa = 3.78 SFGYY406 pKa = 8.75 TDD408 pKa = 4.07 TIVDD412 pKa = 3.57 VRR414 pKa = 11.84 NIIATEE420 pKa = 3.86 FDD422 pKa = 3.67 DD423 pKa = 4.11 EE424 pKa = 5.43 VYY426 pKa = 10.96 GGTHH430 pKa = 6.8 GNTLVLGDD438 pKa = 3.94 GDD440 pKa = 5.44 DD441 pKa = 3.93 YY442 pKa = 12.15 AFGNAGNDD450 pKa = 3.55 TLYY453 pKa = 11.21 GGAGSDD459 pKa = 4.22 RR460 pKa = 11.84 MDD462 pKa = 3.94 GGADD466 pKa = 3.29 TDD468 pKa = 4.27 TVVLGGLNGGGHH480 pKa = 7.62 DD481 pKa = 4.06 LLQFGGVVYY490 pKa = 10.78 ALDD493 pKa = 4.14 LRR495 pKa = 11.84 DD496 pKa = 4.03 HH497 pKa = 6.68 SFDD500 pKa = 3.68 TITNVEE506 pKa = 4.0 QFQGVGQTIGLGAVEE521 pKa = 5.08 GFDD524 pKa = 3.66 PLVYY528 pKa = 10.17 AASYY532 pKa = 10.98 NDD534 pKa = 3.6 LAQVFRR540 pKa = 11.84 FDD542 pKa = 3.83 YY543 pKa = 11.04 NSALSHH549 pKa = 5.27 YY550 pKa = 10.09 VGTGYY555 pKa = 10.91 FEE557 pKa = 4.46 GRR559 pKa = 11.84 EE560 pKa = 4.19 ATFNAAQYY568 pKa = 9.23 LANYY572 pKa = 9.6 QDD574 pKa = 3.6 LQAAFGTNYY583 pKa = 9.47 EE584 pKa = 3.86 AARR587 pKa = 11.84 NHH589 pKa = 6.03 FLASGVDD596 pKa = 3.54 EE597 pKa = 4.87 HH598 pKa = 7.52 RR599 pKa = 11.84 LAEE602 pKa = 4.74 DD603 pKa = 3.57 PLDD606 pKa = 4.29 YY607 pKa = 10.48 IASYY611 pKa = 11.17 GDD613 pKa = 4.52 LIQAFGGQSQSDD625 pKa = 3.77 LVAAGLNHH633 pKa = 5.41 YY634 pKa = 9.42 RR635 pKa = 11.84 AAGFDD640 pKa = 3.09 EE641 pKa = 4.45 GRR643 pKa = 11.84 RR644 pKa = 11.84 GGIDD648 pKa = 3.6 FDD650 pKa = 3.74 VGQYY654 pKa = 10.5 LEE656 pKa = 4.89 NYY658 pKa = 10.03 GDD660 pKa = 3.75 LQSAFGGDD668 pKa = 3.34 GDD670 pKa = 4.61 AATAHH675 pKa = 6.48 YY676 pKa = 10.34 INAGYY681 pKa = 10.45 GEE683 pKa = 4.51 HH684 pKa = 7.23 RR685 pKa = 11.84 LAEE688 pKa = 4.51 DD689 pKa = 3.66 PLDD692 pKa = 4.29 YY693 pKa = 10.48 IASYY697 pKa = 11.12 GDD699 pKa = 3.96 LIEE702 pKa = 5.37 AFGSGNEE709 pKa = 4.0 AQIIQAGLVHH719 pKa = 5.91 YY720 pKa = 10.4 QSGGWSEE727 pKa = 4.19 GRR729 pKa = 11.84 EE730 pKa = 3.67 ADD732 pKa = 4.0 FDD734 pKa = 4.21 VDD736 pKa = 4.8 SYY738 pKa = 11.36 LANYY742 pKa = 9.69 DD743 pKa = 3.64 DD744 pKa = 5.23 LRR746 pKa = 11.84 AAFADD751 pKa = 4.24 GAGGYY756 pKa = 9.85 DD757 pKa = 3.44 EE758 pKa = 5.38 EE759 pKa = 5.3 AALLHH764 pKa = 6.67 FIHH767 pKa = 7.09 AGYY770 pKa = 11.11 AEE772 pKa = 4.34 GRR774 pKa = 11.84 TDD776 pKa = 5.22 DD777 pKa = 5.36 LMAAA781 pKa = 4.43
Molecular weight: 81.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.727
IPC2_protein 3.592
IPC_protein 3.656
Toseland 3.414
ProMoST 3.834
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.617
Rodwell 3.478
Grimsley 3.312
Solomon 3.668
Lehninger 3.617
Nozaki 3.77
DTASelect 4.075
Thurlkill 3.478
EMBOSS 3.63
Sillero 3.783
Patrickios 0.642
IPC_peptide 3.656
IPC2_peptide 3.757
IPC2.peptide.svr19 3.707
Protein with the highest isoelectric point:
>tr|A0A1T4Q481|A0A1T4Q481_9RHIZ Methyltransferase domain-containing protein OS=Consotaella salsifontis OX=1365950 GN=SAMN05428963_104340 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.73 LVRR12 pKa = 11.84 KK13 pKa = 9.15 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATTGGRR28 pKa = 11.84 KK29 pKa = 9.29 VIAARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.03 RR41 pKa = 11.84 LSAA44 pKa = 4.03
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4266
0
4266
1357347
24
2696
318.2
34.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.711 ± 0.049
0.826 ± 0.012
5.699 ± 0.033
6.199 ± 0.036
3.771 ± 0.024
8.504 ± 0.035
1.959 ± 0.019
5.2 ± 0.027
3.208 ± 0.028
10.083 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.464 ± 0.018
2.382 ± 0.021
5.098 ± 0.03
2.894 ± 0.024
7.325 ± 0.047
5.736 ± 0.027
5.122 ± 0.026
7.51 ± 0.031
1.227 ± 0.016
2.081 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here