Hydrangea ringspot virus
Average proteome isoelectric point is 7.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q5DKR0|Q5DKR0_9VIRU Movement protein TGBp3 OS=Hydrangea ringspot virus OX=112228 GN=TGBp3 PE=3 SV=1
MM1 pKa = 6.92 TTTTTTNPIPPQAGTSTPSAAFAPLSQEE29 pKa = 3.98 VLEE32 pKa = 4.37 TLSFPVTSNLVPSPTEE48 pKa = 3.41 LAAIADD54 pKa = 3.76 AWTTLGVPAAEE65 pKa = 4.39 TTKK68 pKa = 10.7 HH69 pKa = 6.18 ALALVNFCFDD79 pKa = 3.81 SGSSATTRR87 pKa = 11.84 FTGASPTPTIPLSALAGAVLEE108 pKa = 4.43 LVPIRR113 pKa = 11.84 KK114 pKa = 8.36 FCRR117 pKa = 11.84 YY118 pKa = 7.69 FAQYY122 pKa = 9.06 IWNARR127 pKa = 11.84 LTANAPPASWEE138 pKa = 3.69 AWNFPEE144 pKa = 4.32 NEE146 pKa = 4.07 KK147 pKa = 10.72 FAGFDD152 pKa = 3.4 FFDD155 pKa = 4.24 GVLNTASLKK164 pKa = 10.39 PPQGLVRR171 pKa = 11.84 KK172 pKa = 7.73 PTEE175 pKa = 3.85 AEE177 pKa = 3.72 RR178 pKa = 11.84 VANATAKK185 pKa = 10.4 SLHH188 pKa = 6.23 LFEE191 pKa = 6.24 AATQRR196 pKa = 11.84 SNLASTATQFTRR208 pKa = 11.84 GRR210 pKa = 11.84 LTDD213 pKa = 3.76 TSPTIQFLPAPEE225 pKa = 3.76
Molecular weight: 23.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.545
IPC2_protein 5.639
IPC_protein 5.55
Toseland 5.69
ProMoST 5.448
Dawson 5.639
Bjellqvist 5.741
Wikipedia 5.55
Rodwell 5.601
Grimsley 5.715
Solomon 5.639
Lehninger 5.614
Nozaki 5.817
DTASelect 5.957
Thurlkill 5.842
EMBOSS 5.779
Sillero 5.919
Patrickios 4.495
IPC_peptide 5.652
IPC2_peptide 5.919
IPC2.peptide.svr19 6.053
Protein with the highest isoelectric point:
>tr|Q5DKQ9|Q5DKQ9_9VIRU Coat protein OS=Hydrangea ringspot virus OX=112228 PE=3 SV=1
MM1 pKa = 7.98 PGPPSEE7 pKa = 4.25 SRR9 pKa = 11.84 RR10 pKa = 11.84 QKK12 pKa = 10.68 RR13 pKa = 11.84 PSTPSLWSTSVLTRR27 pKa = 11.84 ALPQPPASLEE37 pKa = 4.07 PPPPPPSPCPPWPEE51 pKa = 4.63 PFWSWYY57 pKa = 8.93 PSEE60 pKa = 4.93 SSVGTSPSTSGMLASPPTPPRR81 pKa = 11.84 LHH83 pKa = 6.47 GKK85 pKa = 10.04 LGISQRR91 pKa = 11.84 TRR93 pKa = 11.84 SLPASISSMGSSTRR107 pKa = 11.84 PPSSHH112 pKa = 6.7 PRR114 pKa = 11.84 GSSGSPRR121 pKa = 11.84 KK122 pKa = 9.87 QNGSPTQPPSPSTSLRR138 pKa = 11.84 QPPSAPTSRR147 pKa = 11.84 PPPPSLPVGDD157 pKa = 4.15
Molecular weight: 16.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.152
IPC2_protein 10.131
IPC_protein 11.564
Toseland 11.725
ProMoST 12.208
Dawson 11.725
Bjellqvist 11.711
Wikipedia 12.193
Rodwell 11.447
Grimsley 11.769
Solomon 12.208
Lehninger 12.106
Nozaki 11.725
DTASelect 11.711
Thurlkill 11.725
EMBOSS 12.223
Sillero 11.725
Patrickios 11.199
IPC_peptide 12.208
IPC2_peptide 11.199
IPC2.peptide.svr19 9.479
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2187
73
1386
364.5
40.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.871 ± 1.467
1.463 ± 0.277
3.429 ± 0.558
5.761 ± 1.447
4.024 ± 0.737
5.03 ± 0.322
3.292 ± 0.633
4.115 ± 0.805
5.121 ± 1.472
11.248 ± 0.966
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.738 ± 0.506
2.743 ± 0.505
9.968 ± 3.142
4.024 ± 0.617
5.213 ± 0.634
7.819 ± 2.655
7.727 ± 1.186
4.435 ± 0.451
1.189 ± 0.323
2.789 ± 0.785
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here