Amino acid dipepetide frequency for Bifidobacterium phage Bbif-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.072AlaAla: 15.072 ± 2.156
1.101AlaCys: 1.101 ± 0.314
9.399AlaAsp: 9.399 ± 1.019
6.689AlaGlu: 6.689 ± 0.967
1.948AlaPhe: 1.948 ± 0.332
9.822AlaGly: 9.822 ± 1.404
1.355AlaHis: 1.355 ± 0.339
5.843AlaIle: 5.843 ± 0.589
4.911AlaLys: 4.911 ± 0.744
9.399AlaLeu: 9.399 ± 1.174
3.218AlaMet: 3.218 ± 0.587
3.472AlaAsn: 3.472 ± 0.465
2.964AlaPro: 2.964 ± 0.544
4.318AlaGln: 4.318 ± 0.832
7.197AlaArg: 7.197 ± 1.002
7.113AlaSer: 7.113 ± 0.878
8.467AlaThr: 8.467 ± 1.423
5.927AlaVal: 5.927 ± 0.758
2.286AlaTrp: 2.286 ± 0.597
3.048AlaTyr: 3.048 ± 0.521
0.0AlaXaa: 0.0 ± 0.0
Cys
1.355CysAla: 1.355 ± 0.382
0.169CysCys: 0.169 ± 0.115
0.762CysAsp: 0.762 ± 0.226
0.677CysGlu: 0.677 ± 0.229
0.169CysPhe: 0.169 ± 0.101
1.355CysGly: 1.355 ± 0.345
0.508CysHis: 0.508 ± 0.214
0.423CysIle: 0.423 ± 0.226
0.423CysLys: 0.423 ± 0.187
0.423CysLeu: 0.423 ± 0.192
0.0CysMet: 0.0 ± 0.0
0.169CysAsn: 0.169 ± 0.104
0.593CysPro: 0.593 ± 0.224
0.423CysGln: 0.423 ± 0.18
1.27CysArg: 1.27 ± 0.351
0.677CysSer: 0.677 ± 0.228
0.931CysThr: 0.931 ± 0.345
0.593CysVal: 0.593 ± 0.196
0.169CysTrp: 0.169 ± 0.116
0.085CysTyr: 0.085 ± 0.085
0.0CysXaa: 0.0 ± 0.0
Asp
10.076AspAla: 10.076 ± 1.304
1.185AspCys: 1.185 ± 0.468
4.742AspAsp: 4.742 ± 0.806
3.218AspGlu: 3.218 ± 0.607
1.693AspPhe: 1.693 ± 0.378
7.705AspGly: 7.705 ± 0.948
1.101AspHis: 1.101 ± 0.391
4.572AspIle: 4.572 ± 0.551
2.202AspLys: 2.202 ± 0.399
5.504AspLeu: 5.504 ± 0.654
2.371AspMet: 2.371 ± 0.516
2.202AspAsn: 2.202 ± 0.364
3.726AspPro: 3.726 ± 0.508
2.117AspGln: 2.117 ± 0.346
5.165AspArg: 5.165 ± 0.632
2.964AspSer: 2.964 ± 0.438
3.641AspThr: 3.641 ± 0.572
3.556AspVal: 3.556 ± 0.56
2.456AspTrp: 2.456 ± 0.48
1.609AspTyr: 1.609 ± 0.332
0.0AspXaa: 0.0 ± 0.0
Glu
6.435GluAla: 6.435 ± 0.668
0.677GluCys: 0.677 ± 0.277
3.133GluAsp: 3.133 ± 0.625
3.048GluGlu: 3.048 ± 0.637
1.185GluPhe: 1.185 ± 0.256
3.556GluGly: 3.556 ± 0.663
2.371GluHis: 2.371 ± 0.559
2.032GluIle: 2.032 ± 0.423
2.794GluLys: 2.794 ± 0.442
5.588GluLeu: 5.588 ± 0.601
1.439GluMet: 1.439 ± 0.345
1.693GluAsn: 1.693 ± 0.405
2.625GluPro: 2.625 ± 0.54
2.879GluGln: 2.879 ± 0.471
4.403GluArg: 4.403 ± 0.986
3.387GluSer: 3.387 ± 0.51
4.318GluThr: 4.318 ± 0.616
3.048GluVal: 3.048 ± 0.476
0.931GluTrp: 0.931 ± 0.31
1.439GluTyr: 1.439 ± 0.302
0.0GluXaa: 0.0 ± 0.0
Phe
2.794PheAla: 2.794 ± 0.581
0.423PheCys: 0.423 ± 0.172
2.456PheAsp: 2.456 ± 0.417
0.847PheGlu: 0.847 ± 0.309
0.169PhePhe: 0.169 ± 0.104
2.202PheGly: 2.202 ± 0.436
0.423PheHis: 0.423 ± 0.244
0.931PheIle: 0.931 ± 0.25
0.423PheLys: 0.423 ± 0.212
1.355PheLeu: 1.355 ± 0.317
0.508PheMet: 0.508 ± 0.215
0.593PheAsn: 0.593 ± 0.187
0.677PhePro: 0.677 ± 0.218
0.593PheGln: 0.593 ± 0.258
1.27PheArg: 1.27 ± 0.379
1.609PheSer: 1.609 ± 0.329
1.439PheThr: 1.439 ± 0.316
1.016PheVal: 1.016 ± 0.284
0.593PheTrp: 0.593 ± 0.258
0.677PheTyr: 0.677 ± 0.234
0.0PheXaa: 0.0 ± 0.0
Gly
5.843GlyAla: 5.843 ± 0.789
0.762GlyCys: 0.762 ± 0.239
5.588GlyAsp: 5.588 ± 0.868
3.81GlyGlu: 3.81 ± 0.477
1.778GlyPhe: 1.778 ± 0.417
7.028GlyGly: 7.028 ± 0.848
1.439GlyHis: 1.439 ± 0.366
4.572GlyIle: 4.572 ± 0.769
3.641GlyLys: 3.641 ± 0.606
7.113GlyLeu: 7.113 ± 0.72
3.218GlyMet: 3.218 ± 0.703
2.286GlyAsn: 2.286 ± 0.396
1.948GlyPro: 1.948 ± 0.301
3.302GlyGln: 3.302 ± 0.568
7.282GlyArg: 7.282 ± 0.901
4.826GlySer: 4.826 ± 0.781
6.266GlyThr: 6.266 ± 0.959
6.266GlyVal: 6.266 ± 1.037
1.863GlyTrp: 1.863 ± 0.482
2.964GlyTyr: 2.964 ± 0.574
0.0GlyXaa: 0.0 ± 0.0
His
2.286HisAla: 2.286 ± 0.412
0.508HisCys: 0.508 ± 0.22
1.524HisAsp: 1.524 ± 0.369
1.439HisGlu: 1.439 ± 0.385
0.254HisPhe: 0.254 ± 0.143
1.693HisGly: 1.693 ± 0.482
0.677HisHis: 0.677 ± 0.278
1.27HisIle: 1.27 ± 0.37
0.593HisLys: 0.593 ± 0.266
1.27HisLeu: 1.27 ± 0.289
0.508HisMet: 0.508 ± 0.191
0.254HisAsn: 0.254 ± 0.144
0.847HisPro: 0.847 ± 0.28
0.762HisGln: 0.762 ± 0.241
1.778HisArg: 1.778 ± 0.376
0.931HisSer: 0.931 ± 0.279
1.355HisThr: 1.355 ± 0.34
0.847HisVal: 0.847 ± 0.264
0.508HisTrp: 0.508 ± 0.22
0.508HisTyr: 0.508 ± 0.222
0.0HisXaa: 0.0 ± 0.0
Ile
6.351IleAla: 6.351 ± 0.738
0.593IleCys: 0.593 ± 0.23
3.726IleAsp: 3.726 ± 0.61
3.81IleGlu: 3.81 ± 0.472
0.339IlePhe: 0.339 ± 0.144
3.81IleGly: 3.81 ± 0.532
0.931IleHis: 0.931 ± 0.243
2.286IleIle: 2.286 ± 0.461
2.117IleLys: 2.117 ± 0.477
3.048IleLeu: 3.048 ± 0.461
1.185IleMet: 1.185 ± 0.284
1.948IleAsn: 1.948 ± 0.463
2.879IlePro: 2.879 ± 0.501
1.693IleGln: 1.693 ± 0.391
3.98IleArg: 3.98 ± 0.551
3.048IleSer: 3.048 ± 0.55
4.149IleThr: 4.149 ± 0.786
2.625IleVal: 2.625 ± 0.444
0.677IleTrp: 0.677 ± 0.218
0.762IleTyr: 0.762 ± 0.326
0.0IleXaa: 0.0 ± 0.0
Lys
5.843LysAla: 5.843 ± 0.761
0.085LysCys: 0.085 ± 0.083
2.71LysAsp: 2.71 ± 0.416
2.964LysGlu: 2.964 ± 0.712
1.185LysPhe: 1.185 ± 0.336
3.472LysGly: 3.472 ± 0.427
0.677LysHis: 0.677 ± 0.227
1.185LysIle: 1.185 ± 0.317
1.609LysLys: 1.609 ± 0.407
3.302LysLeu: 3.302 ± 0.468
1.185LysMet: 1.185 ± 0.322
1.355LysAsn: 1.355 ± 0.381
3.387LysPro: 3.387 ± 0.56
1.439LysGln: 1.439 ± 0.309
3.048LysArg: 3.048 ± 0.575
1.439LysSer: 1.439 ± 0.29
4.911LysThr: 4.911 ± 0.713
2.032LysVal: 2.032 ± 0.312
0.762LysTrp: 0.762 ± 0.306
0.677LysTyr: 0.677 ± 0.224
0.0LysXaa: 0.0 ± 0.0
Leu
9.992LeuAla: 9.992 ± 1.046
0.847LeuCys: 0.847 ± 0.312
7.282LeuAsp: 7.282 ± 0.961
4.318LeuGlu: 4.318 ± 0.647
1.27LeuPhe: 1.27 ± 0.339
6.351LeuGly: 6.351 ± 0.756
1.693LeuHis: 1.693 ± 0.379
3.98LeuIle: 3.98 ± 0.529
3.218LeuLys: 3.218 ± 0.479
5.08LeuLeu: 5.08 ± 0.706
1.778LeuMet: 1.778 ± 0.366
3.726LeuAsn: 3.726 ± 0.545
4.318LeuPro: 4.318 ± 0.571
2.286LeuGln: 2.286 ± 0.481
6.181LeuArg: 6.181 ± 0.79
4.318LeuSer: 4.318 ± 0.69
6.859LeuThr: 6.859 ± 0.607
3.556LeuVal: 3.556 ± 0.542
1.693LeuTrp: 1.693 ± 0.459
1.778LeuTyr: 1.778 ± 0.363
0.0LeuXaa: 0.0 ± 0.0
Met
3.133MetAla: 3.133 ± 0.539
0.339MetCys: 0.339 ± 0.15
1.778MetAsp: 1.778 ± 0.395
1.863MetGlu: 1.863 ± 0.535
0.931MetPhe: 0.931 ± 0.277
1.355MetGly: 1.355 ± 0.391
0.169MetHis: 0.169 ± 0.13
1.185MetIle: 1.185 ± 0.292
0.931MetLys: 0.931 ± 0.261
2.371MetLeu: 2.371 ± 0.358
0.593MetMet: 0.593 ± 0.259
0.847MetAsn: 0.847 ± 0.219
1.524MetPro: 1.524 ± 0.387
0.593MetGln: 0.593 ± 0.242
2.456MetArg: 2.456 ± 0.444
3.048MetSer: 3.048 ± 0.59
2.286MetThr: 2.286 ± 0.427
1.185MetVal: 1.185 ± 0.297
0.0MetTrp: 0.0 ± 0.0
0.339MetTyr: 0.339 ± 0.2
0.0MetXaa: 0.0 ± 0.0
Asn
4.403AsnAla: 4.403 ± 0.629
0.169AsnCys: 0.169 ± 0.149
2.286AsnAsp: 2.286 ± 0.523
1.778AsnGlu: 1.778 ± 0.41
0.508AsnPhe: 0.508 ± 0.173
3.133AsnGly: 3.133 ± 0.49
0.677AsnHis: 0.677 ± 0.231
0.931AsnIle: 0.931 ± 0.261
0.677AsnLys: 0.677 ± 0.215
3.895AsnLeu: 3.895 ± 0.489
0.847AsnMet: 0.847 ± 0.262
1.016AsnAsn: 1.016 ± 0.313
2.202AsnPro: 2.202 ± 0.376
1.439AsnGln: 1.439 ± 0.298
1.693AsnArg: 1.693 ± 0.336
1.524AsnSer: 1.524 ± 0.421
2.456AsnThr: 2.456 ± 0.49
1.948AsnVal: 1.948 ± 0.414
0.423AsnTrp: 0.423 ± 0.168
0.593AsnTyr: 0.593 ± 0.228
0.0AsnXaa: 0.0 ± 0.0
Pro
4.657ProAla: 4.657 ± 0.691
0.423ProCys: 0.423 ± 0.216
3.81ProAsp: 3.81 ± 0.606
3.302ProGlu: 3.302 ± 0.627
0.931ProPhe: 0.931 ± 0.325
4.318ProGly: 4.318 ± 0.565
0.847ProHis: 0.847 ± 0.227
2.71ProIle: 2.71 ± 0.534
1.609ProLys: 1.609 ± 0.414
3.133ProLeu: 3.133 ± 0.578
1.016ProMet: 1.016 ± 0.269
1.185ProAsn: 1.185 ± 0.278
1.101ProPro: 1.101 ± 0.321
1.439ProGln: 1.439 ± 0.312
2.456ProArg: 2.456 ± 0.466
2.456ProSer: 2.456 ± 0.455
2.964ProThr: 2.964 ± 0.572
2.964ProVal: 2.964 ± 0.514
0.847ProTrp: 0.847 ± 0.265
1.439ProTyr: 1.439 ± 0.345
0.0ProXaa: 0.0 ± 0.0
Gln
4.572GlnAla: 4.572 ± 0.772
0.339GlnCys: 0.339 ± 0.159
1.863GlnAsp: 1.863 ± 0.402
1.439GlnGlu: 1.439 ± 0.289
0.508GlnPhe: 0.508 ± 0.199
2.371GlnGly: 2.371 ± 0.398
0.762GlnHis: 0.762 ± 0.27
2.032GlnIle: 2.032 ± 0.375
1.101GlnLys: 1.101 ± 0.331
3.895GlnLeu: 3.895 ± 0.567
0.593GlnMet: 0.593 ± 0.234
1.355GlnAsn: 1.355 ± 0.335
2.371GlnPro: 2.371 ± 0.369
1.863GlnGln: 1.863 ± 0.655
3.048GlnArg: 3.048 ± 0.535
2.54GlnSer: 2.54 ± 0.576
2.879GlnThr: 2.879 ± 0.504
2.032GlnVal: 2.032 ± 0.393
1.016GlnTrp: 1.016 ± 0.304
0.677GlnTyr: 0.677 ± 0.217
0.0GlnXaa: 0.0 ± 0.0
Arg
6.689ArgAla: 6.689 ± 0.823
0.762ArgCys: 0.762 ± 0.302
4.488ArgAsp: 4.488 ± 0.563
5.25ArgGlu: 5.25 ± 0.924
2.456ArgPhe: 2.456 ± 0.503
4.403ArgGly: 4.403 ± 0.736
1.439ArgHis: 1.439 ± 0.384
4.742ArgIle: 4.742 ± 0.638
4.996ArgLys: 4.996 ± 0.737
7.197ArgLeu: 7.197 ± 0.862
2.54ArgMet: 2.54 ± 0.658
2.202ArgAsn: 2.202 ± 0.362
2.964ArgPro: 2.964 ± 0.662
3.048ArgGln: 3.048 ± 0.448
8.467ArgArg: 8.467 ± 1.213
3.556ArgSer: 3.556 ± 0.661
4.742ArgThr: 4.742 ± 0.614
3.556ArgVal: 3.556 ± 0.541
1.948ArgTrp: 1.948 ± 0.404
2.117ArgTyr: 2.117 ± 0.416
0.0ArgXaa: 0.0 ± 0.0
Ser
4.826SerAla: 4.826 ± 0.876
0.762SerCys: 0.762 ± 0.296
4.064SerAsp: 4.064 ± 0.556
3.556SerGlu: 3.556 ± 0.657
1.778SerPhe: 1.778 ± 0.413
4.826SerGly: 4.826 ± 0.917
0.931SerHis: 0.931 ± 0.258
2.032SerIle: 2.032 ± 0.533
3.218SerLys: 3.218 ± 0.468
4.572SerLeu: 4.572 ± 0.631
1.778SerMet: 1.778 ± 0.548
2.371SerAsn: 2.371 ± 0.403
1.778SerPro: 1.778 ± 0.449
3.133SerGln: 3.133 ± 0.453
4.657SerArg: 4.657 ± 0.587
3.98SerSer: 3.98 ± 0.805
3.641SerThr: 3.641 ± 0.621
3.218SerVal: 3.218 ± 0.488
0.931SerTrp: 0.931 ± 0.293
1.524SerTyr: 1.524 ± 0.327
0.0SerXaa: 0.0 ± 0.0
Thr
8.806ThrAla: 8.806 ± 1.204
0.762ThrCys: 0.762 ± 0.247
5.08ThrAsp: 5.08 ± 0.632
3.641ThrGlu: 3.641 ± 0.495
1.101ThrPhe: 1.101 ± 0.317
6.859ThrGly: 6.859 ± 0.819
1.524ThrHis: 1.524 ± 0.419
5.165ThrIle: 5.165 ± 0.605
3.133ThrLys: 3.133 ± 0.575
5.927ThrLeu: 5.927 ± 1.034
1.524ThrMet: 1.524 ± 0.448
1.609ThrAsn: 1.609 ± 0.326
4.064ThrPro: 4.064 ± 0.639
2.54ThrGln: 2.54 ± 0.406
5.25ThrArg: 5.25 ± 0.769
4.318ThrSer: 4.318 ± 0.721
5.25ThrThr: 5.25 ± 1.04
5.25ThrVal: 5.25 ± 0.718
1.693ThrTrp: 1.693 ± 0.359
1.27ThrTyr: 1.27 ± 0.396
0.0ThrXaa: 0.0 ± 0.0
Val
6.774ValAla: 6.774 ± 0.877
0.847ValCys: 0.847 ± 0.279
3.895ValAsp: 3.895 ± 0.532
2.71ValGlu: 2.71 ± 0.466
1.439ValPhe: 1.439 ± 0.372
3.302ValGly: 3.302 ± 0.465
0.677ValHis: 0.677 ± 0.214
2.371ValIle: 2.371 ± 0.423
3.726ValLys: 3.726 ± 0.644
3.556ValLeu: 3.556 ± 0.537
1.27ValMet: 1.27 ± 0.302
1.609ValAsn: 1.609 ± 0.303
2.286ValPro: 2.286 ± 0.49
2.202ValGln: 2.202 ± 0.634
3.81ValArg: 3.81 ± 0.544
4.234ValSer: 4.234 ± 0.507
5.08ValThr: 5.08 ± 0.705
2.964ValVal: 2.964 ± 0.6
0.593ValTrp: 0.593 ± 0.216
1.355ValTyr: 1.355 ± 0.319
0.0ValXaa: 0.0 ± 0.0
Trp
1.355TrpAla: 1.355 ± 0.257
0.254TrpCys: 0.254 ± 0.146
1.778TrpAsp: 1.778 ± 0.417
1.101TrpGlu: 1.101 ± 0.288
0.593TrpPhe: 0.593 ± 0.229
1.185TrpGly: 1.185 ± 0.362
1.185TrpHis: 1.185 ± 0.319
0.508TrpIle: 0.508 ± 0.179
1.016TrpLys: 1.016 ± 0.319
2.202TrpLeu: 2.202 ± 0.441
0.762TrpMet: 0.762 ± 0.295
1.609TrpAsn: 1.609 ± 0.368
0.593TrpPro: 0.593 ± 0.278
0.508TrpGln: 0.508 ± 0.186
2.286TrpArg: 2.286 ± 0.537
0.593TrpSer: 0.593 ± 0.306
1.101TrpThr: 1.101 ± 0.284
0.931TrpVal: 0.931 ± 0.249
0.677TrpTrp: 0.677 ± 0.253
0.677TrpTyr: 0.677 ± 0.24
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.286TyrAla: 2.286 ± 0.411
0.169TyrCys: 0.169 ± 0.106
1.439TyrAsp: 1.439 ± 0.354
1.524TyrGlu: 1.524 ± 0.273
0.762TyrPhe: 0.762 ± 0.282
3.048TyrGly: 3.048 ± 0.469
0.423TyrHis: 0.423 ± 0.195
1.185TyrIle: 1.185 ± 0.485
0.931TyrLys: 0.931 ± 0.305
1.693TyrLeu: 1.693 ± 0.432
0.508TyrMet: 0.508 ± 0.213
1.101TyrAsn: 1.101 ± 0.332
0.762TyrPro: 0.762 ± 0.259
0.762TyrGln: 0.762 ± 0.281
1.863TyrArg: 1.863 ± 0.398
1.016TyrSer: 1.016 ± 0.306
2.032TyrThr: 2.032 ± 0.344
1.185TyrVal: 1.185 ± 0.304
0.847TyrTrp: 0.847 ± 0.293
0.677TyrTyr: 0.677 ± 0.332
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 61 proteins (11811 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski