Verrucomicrobia bacterium S94

Taxonomy: cellular organisms; Bacteria; PVC group; Verrucomicrobia; unclassified Verrucomicrobia

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3458 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P6PGC5|A0A4P6PGC5_9BACT SPFH/Band 7/PHB domain protein OS=Verrucomicrobia bacterium S94 OX=2488809 GN=EGM51_09315 PE=3 SV=1
MM1 pKa = 6.92MRR3 pKa = 11.84VLKK6 pKa = 10.52ILGLSVICSGVVLAQYY22 pKa = 10.48TNVIEE27 pKa = 4.89DD28 pKa = 3.69GQTIQVVSDD37 pKa = 3.67WDD39 pKa = 3.51NGGADD44 pKa = 4.96LIVGGTSGGNTLYY57 pKa = 10.5INTGYY62 pKa = 10.5VVSNSSEE69 pKa = 4.08VVIGRR74 pKa = 11.84DD75 pKa = 2.96AGADD79 pKa = 3.64EE80 pKa = 4.82NRR82 pKa = 11.84VDD84 pKa = 4.95LDD86 pKa = 3.76GSGLLVNGDD95 pKa = 3.96MIVGGEE101 pKa = 4.07GDD103 pKa = 4.24GNLLLIDD110 pKa = 3.7NDD112 pKa = 4.27SYY114 pKa = 12.12VEE116 pKa = 3.89NSNAYY121 pKa = 10.07VGVNSEE127 pKa = 4.37DD128 pKa = 3.46NAVAVTDD135 pKa = 4.13DD136 pKa = 4.61SVWNNNGTLYY146 pKa = 10.79LDD148 pKa = 4.0SGSGNAVSVSSGGQVLADD166 pKa = 3.68SLQLSGDD173 pKa = 3.39NVFNLNNSGVLLLRR187 pKa = 11.84SDD189 pKa = 4.63FNVSTSGFNWNTGGHH204 pKa = 7.07LYY206 pKa = 8.74LTNGTLTGLTDD217 pKa = 3.76TNGATYY223 pKa = 10.67LHH225 pKa = 7.29DD226 pKa = 4.63EE227 pKa = 4.8RR228 pKa = 11.84DD229 pKa = 3.6LTLNDD234 pKa = 3.56ADD236 pKa = 4.22MDD238 pKa = 4.12VSGTDD243 pKa = 3.3LTVGDD248 pKa = 4.93FGSNSDD254 pKa = 3.86LQILNGGTVLADD266 pKa = 3.21NGVIGSGSDD275 pKa = 3.4AEE277 pKa = 4.58DD278 pKa = 3.18NSIIVSGEE286 pKa = 3.75GSSLSFSEE294 pKa = 4.65GGLQIGVNAGTGNALGVFDD313 pKa = 4.92GAWTFVGDD321 pKa = 4.3AGTNDD326 pKa = 3.17VAGTTNGGLIVAHH339 pKa = 6.42SSGATLEE346 pKa = 4.49LDD348 pKa = 3.13NSAVVSVDD356 pKa = 2.51GGLFVGTAGGGTGAVTIANGSSVGVGTVEE385 pKa = 5.0IEE387 pKa = 4.35DD388 pKa = 3.91GSQIEE393 pKa = 4.1LQEE396 pKa = 3.88YY397 pKa = 10.04GAFLVEE403 pKa = 4.27GDD405 pKa = 4.78FIYY408 pKa = 10.98GNDD411 pKa = 3.06SSKK414 pKa = 10.61FVWEE418 pKa = 4.99DD419 pKa = 3.36YY420 pKa = 7.71TTLGVGGTLSKK431 pKa = 10.23TNGLDD436 pKa = 3.34GTQRR440 pKa = 11.84TLNLNGGDD448 pKa = 3.35WALADD453 pKa = 3.63STVISGISNTVQVLDD468 pKa = 4.3GGTLTTTNGLLSGTNNAVVISGTDD492 pKa = 3.4SHH494 pKa = 6.6WEE496 pKa = 3.98NAGSLYY502 pKa = 10.98AEE504 pKa = 4.37GTNNSVSVASGGKK517 pKa = 9.48VSATGVDD524 pKa = 4.44FEE526 pKa = 6.07DD527 pKa = 4.51GNAFNINSGGTLALTTNLFDD547 pKa = 3.46WSAVEE552 pKa = 4.07NINWNSGGNLALIDD566 pKa = 4.03GEE568 pKa = 4.47ITGLDD573 pKa = 3.38STNLVFNGSTNSAVSVGGGRR593 pKa = 11.84SLTLEE598 pKa = 4.01NGTWDD603 pKa = 4.68DD604 pKa = 3.54NSADD608 pKa = 3.73HH609 pKa = 6.85LVVGLNQSGEE619 pKa = 4.29TVAISNKK626 pKa = 6.13TTMVHH631 pKa = 5.56NDD633 pKa = 3.23AYY635 pKa = 9.89IGWGSGSEE643 pKa = 4.25GNSVVVSDD651 pKa = 6.07ADD653 pKa = 4.03TLWEE657 pKa = 4.14VQGGDD662 pKa = 4.25LYY664 pKa = 10.78IGAYY668 pKa = 8.36WVDD671 pKa = 3.73GTNLTATGGNDD682 pKa = 2.66NYY684 pKa = 11.27LYY686 pKa = 10.93VRR688 pKa = 11.84DD689 pKa = 3.9GAQVFVGDD697 pKa = 3.87TTTNEE702 pKa = 3.85AGVLVASSDD711 pKa = 3.72GATMTLGDD719 pKa = 4.2GRR721 pKa = 11.84VEE723 pKa = 3.86IDD725 pKa = 3.18DD726 pKa = 4.19TLFIGQGVNTGIVTIMDD743 pKa = 4.13GGDD746 pKa = 3.41MRR748 pKa = 11.84VGDD751 pKa = 4.45LKK753 pKa = 10.73IAAGSEE759 pKa = 3.85LDD761 pKa = 3.66LQEE764 pKa = 4.59NGIFRR769 pKa = 11.84ISTNLNMDD777 pKa = 3.91VLAGNGFDD785 pKa = 3.38WYY787 pKa = 9.59STNSTVQVDD796 pKa = 4.05GTLTRR801 pKa = 11.84SDD803 pKa = 3.66YY804 pKa = 11.22FLDD807 pKa = 3.9KK808 pKa = 10.54EE809 pKa = 4.25QNLILGTNGLWDD821 pKa = 3.38TGGADD826 pKa = 5.08LIVGVDD832 pKa = 3.94GNSKK836 pKa = 8.89LTVRR840 pKa = 11.84DD841 pKa = 3.57TGEE844 pKa = 4.02LNAGNITIGGSVSNGGSSAVIVDD867 pKa = 3.68GGVLNGTDD875 pKa = 3.75VIIGADD881 pKa = 3.15TDD883 pKa = 3.75EE884 pKa = 5.58NYY886 pKa = 9.64MEE888 pKa = 5.44ISDD891 pKa = 3.79QGSVNLLGSVYY902 pKa = 10.69LGGTNSTGNYY912 pKa = 9.41VLLDD916 pKa = 3.8GSNTTMNIGGDD927 pKa = 3.85LWVGAADD934 pKa = 3.99TNSSGNIFYY943 pKa = 10.43LQNQAEE949 pKa = 4.48LTVDD953 pKa = 2.94GDD955 pKa = 4.02VFVFGGNRR963 pKa = 11.84MVLSDD968 pKa = 3.29GTNFVGGDD976 pKa = 3.26FTVYY980 pKa = 10.85SNAALTGTGEE990 pKa = 5.02IEE992 pKa = 4.1LTDD995 pKa = 4.03ANALLTLEE1003 pKa = 4.75NGNMAIDD1010 pKa = 4.01EE1011 pKa = 4.97DD1012 pKa = 4.56GSLGYY1017 pKa = 8.44TVSSGVSFAGTGSNQVKK1034 pKa = 10.09VDD1036 pKa = 3.32GGIFGLLGSSTADD1049 pKa = 3.07QYY1051 pKa = 12.0SGFDD1055 pKa = 3.38SLTLSGTEE1063 pKa = 4.03FYY1065 pKa = 10.83GYY1067 pKa = 8.39GTNDD1071 pKa = 3.14FNSFSMTNGTIRR1083 pKa = 11.84PSSNLGTTALSGGEE1097 pKa = 3.81YY1098 pKa = 10.46RR1099 pKa = 11.84LIDD1102 pKa = 3.51TAGTLVMNGSFSASNTTYY1120 pKa = 10.7KK1121 pKa = 10.87ADD1123 pKa = 3.15ILGVYY1128 pKa = 9.89GHH1130 pKa = 7.56DD1131 pKa = 3.9LLHH1134 pKa = 6.67FAQNVTLDD1142 pKa = 3.81GLIADD1147 pKa = 3.9ITVASVTNSEE1157 pKa = 4.13MVILSSDD1164 pKa = 3.32VGLSGDD1170 pKa = 3.59FAEE1173 pKa = 4.79TNLTNHH1179 pKa = 6.4MLLFDD1184 pKa = 4.5ASLVKK1189 pKa = 10.65VGNDD1193 pKa = 2.74VVVQMTNTADD1203 pKa = 3.67SFSSSLDD1210 pKa = 3.52YY1211 pKa = 11.21AGSEE1215 pKa = 4.42SIRR1218 pKa = 11.84AGFNGMKK1225 pKa = 10.15NAVFTRR1231 pKa = 11.84TKK1233 pKa = 9.18QLRR1236 pKa = 11.84RR1237 pKa = 11.84NLVSTAHH1244 pKa = 7.54AIPHH1248 pKa = 6.69DD1249 pKa = 4.41AFLMTNTNAPAGPQGPGADD1268 pKa = 3.16NTIFDD1273 pKa = 3.77MHH1275 pKa = 6.68VWAQYY1280 pKa = 11.19FNGQGDD1286 pKa = 4.03YY1287 pKa = 10.62DD1288 pKa = 3.73AHH1290 pKa = 6.31GLSDD1294 pKa = 5.18GYY1296 pKa = 9.22TLNQSGTTLGADD1308 pKa = 3.2RR1309 pKa = 11.84LVGEE1313 pKa = 4.86DD1314 pKa = 3.29LTVGFNYY1321 pKa = 9.99TYY1323 pKa = 11.37ARR1325 pKa = 11.84GDD1327 pKa = 3.67AEE1329 pKa = 4.57TTNTDD1334 pKa = 3.52YY1335 pKa = 11.67LDD1337 pKa = 3.85TEE1339 pKa = 4.95TYY1341 pKa = 9.24WFGAYY1346 pKa = 10.02GEE1348 pKa = 4.4WVGKK1352 pKa = 10.21SGLYY1356 pKa = 10.26VDD1358 pKa = 4.26TLLAYY1363 pKa = 9.93GRR1365 pKa = 11.84SNYY1368 pKa = 10.55DD1369 pKa = 3.19SIRR1372 pKa = 11.84VEE1374 pKa = 3.9NDD1376 pKa = 2.75DD1377 pKa = 4.08DD1378 pKa = 4.72RR1379 pKa = 11.84DD1380 pKa = 3.82YY1381 pKa = 11.58RR1382 pKa = 11.84GTASYY1387 pKa = 10.64RR1388 pKa = 11.84GDD1390 pKa = 3.44NFGGYY1395 pKa = 9.71VDD1397 pKa = 3.83VGQYY1401 pKa = 9.19MYY1403 pKa = 11.16YY1404 pKa = 10.46KK1405 pKa = 10.06NLAIAPYY1412 pKa = 9.42IGLHH1416 pKa = 5.97ALFMDD1421 pKa = 4.25TDD1423 pKa = 3.81DD1424 pKa = 3.77HH1425 pKa = 6.61TEE1427 pKa = 3.7TSTVDD1432 pKa = 3.03NNEE1435 pKa = 3.81QLKK1438 pKa = 10.11VDD1440 pKa = 3.84GMSRR1444 pKa = 11.84TLVEE1448 pKa = 4.05SALGLKK1454 pKa = 9.64ARR1456 pKa = 11.84HH1457 pKa = 5.99RR1458 pKa = 11.84FDD1460 pKa = 3.14TSIGRR1465 pKa = 11.84FQTTGYY1471 pKa = 11.08AEE1473 pKa = 4.03WTHH1476 pKa = 7.04DD1477 pKa = 3.78FVQDD1481 pKa = 4.27EE1482 pKa = 5.0IATEE1486 pKa = 4.0MQQVNDD1492 pKa = 3.6VLGLNTAPVTQAAIKK1507 pKa = 10.36PEE1509 pKa = 3.91EE1510 pKa = 4.01DD1511 pKa = 3.64LFNIGLGLSWRR1522 pKa = 11.84STEE1525 pKa = 3.8YY1526 pKa = 10.04MEE1528 pKa = 5.92IGIGYY1533 pKa = 9.38NGRR1536 pKa = 11.84FSDD1539 pKa = 5.1DD1540 pKa = 3.79YY1541 pKa = 9.93EE1542 pKa = 4.18EE1543 pKa = 5.47HH1544 pKa = 6.51MGSLMLDD1551 pKa = 3.3VMFF1554 pKa = 5.92

Molecular weight: 162.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P6PKW6|A0A4P6PKW6_9BACT Uncharacterized protein OS=Verrucomicrobia bacterium S94 OX=2488809 GN=EGM51_14435 PE=4 SV=1
MM1 pKa = 7.61LSCKK5 pKa = 9.48EE6 pKa = 3.85VSRR9 pKa = 11.84LISEE13 pKa = 4.21SLDD16 pKa = 3.12RR17 pKa = 11.84KK18 pKa = 10.48LPFRR22 pKa = 11.84QRR24 pKa = 11.84IGVRR28 pKa = 11.84MHH30 pKa = 7.93LMMCSLCRR38 pKa = 11.84RR39 pKa = 11.84YY40 pKa = 9.66RR41 pKa = 11.84AQAVYY46 pKa = 10.45LRR48 pKa = 11.84YY49 pKa = 10.06LFTHH53 pKa = 6.87IAGDD57 pKa = 3.75DD58 pKa = 3.56RR59 pKa = 11.84KK60 pKa = 9.98GTFPEE65 pKa = 4.16EE66 pKa = 4.16RR67 pKa = 11.84LSEE70 pKa = 4.09PARR73 pKa = 11.84EE74 pKa = 3.99RR75 pKa = 11.84IKK77 pKa = 10.9QILRR81 pKa = 11.84NNRR84 pKa = 11.84NN85 pKa = 2.99

Molecular weight: 10.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3458

0

3458

1203030

29

2696

347.9

38.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.639 ± 0.044

1.204 ± 0.014

5.789 ± 0.032

7.076 ± 0.038

4.192 ± 0.027

7.72 ± 0.037

2.137 ± 0.019

6.054 ± 0.033

5.215 ± 0.037

9.277 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.673 ± 0.018

3.918 ± 0.027

4.532 ± 0.031

3.264 ± 0.021

5.587 ± 0.042

5.894 ± 0.036

5.369 ± 0.032

6.815 ± 0.031

1.456 ± 0.022

3.188 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski